FastQCFastQC Report
Sat 31 Dec 2022
SRR5845210.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845210.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7588
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGCCGTCT460.6062203479177649RNA PCR Primer, Index 42 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGC410.540326831839747No Hit
TCAATGGGGGTTCCAGTTGGGATGAAGCATTTCATCTCACTTCATAACACCT210.27675276752767525No Hit
TGCTATGGACCAATGCATCTCTTTAAAGGCAAATATTATCCAGCAAGCAGTC170.22403795466526094No Hit
AATAAAGGGTAATACTAAAGCTTATTGTTAAAGGAAGGCAGAAAAATTAAGT170.22403795466526094No Hit
CAGCAAGGGAGAAACAATCATATATAAAGAGAAACTCTGTAAGTGGAATCAT160.21085925144965736No Hit
TGTTAGGGGACTCTTAAGAAGGGACGGGGCCCCACGCTGCGCACCCGCGGGT160.21085925144965736No Hit
AGCACTGGGCAGAAAAAATCACAATTCTAAATATATATGCACCTAAAACTGG150.19768054823405376No Hit
ACTTGCGGGGCTCTCAGAATCGCTGCCGCCATGGCTAGTCAGTCTCAGGGGA150.19768054823405376No Hit
GTCATAGGGCAAATTGAAAGGATAGCCAGGATGACTAAGACATACTCTTACA140.18450184501845018No Hit
AATCTTGGGAGCCTTTCCTTAAAATTAGACATATTTAGTTCTTCACTCTTCA140.18450184501845018No Hit
TGAAAGGAAGTATGCTGTTCTCTATCAGCCTCTATTTGATAAGCGATTTGAA140.18450184501845018No Hit
TTTCCAGGGCTTCAAGGAAGAGCAAGGCACAGTAAGGCAAATAAATCCTTCC140.18450184501845018No Hit
CAGCTGGGGATGTGATGAATTACGTTTATTGATTTGCATATGTTGAACCAAC140.18450184501845018No Hit
GCAACTGGGATATTACAATCAAATATTGTAATCACTGACACAAGAATCTTAA140.18450184501845018No Hit
TCAGCAAGGGCTGATTCATACAGTTCCAACCTTTTTCTAGTTTCACTTGCTG140.18450184501845018No Hit
AGTTGCGGGACTTGATGATGCTTGGGAAAGAATCAACAGTTAAAACTTCATG140.18450184501845018No Hit
TGCACAGGGTGAAAGAATTATCTAAACGTATATTTGTCAGATCAATACCGTC140.18450184501845018No Hit
AAGGCAGGGGGAGTCTGGCAGCAGACTTCTCAGTGGAAACTCTACAGGCCAG130.1713231418028466No Hit
GCACTCCAGCCTGGACAACATGGCGAAACGCCATCTTTAAAAATAAAATCCA130.1713231418028466No Hit
GAGGCACGAGAATTGCTTGAACCCGCGGCGCGGAGATTGCAGTGGGCCGGGA130.1713231418028466No Hit
ACACTTGGGATGTTCATACCAGTTAAACTGCACTTTCCCAAGGGGACAGCTG130.1713231418028466No Hit
GCACTAGGGTATTGTGTTCCTATATGTTGAATTTTACTGTGTTTTAGTGAAG130.1713231418028466No Hit
TTTTGTGGGGTCCTATGAATTTGGGAACTTCTCTCTTTGGTCATTGTCTTTT130.1713231418028466No Hit
CTCAGGGGGATGAGCGGAGTCTGTCCTCTGCTCTTCTGCAGTCAGGTCACTG130.1713231418028466No Hit
TCCAAGGGGACTACTTTGAAACATAAGTTAAAGCCATTAGCTGTGTTTTTGT120.158144438587243No Hit
TTAGCCGGGTCACCTCAGCCATTTTGGCCCAGGGAAAATAATTTTGCTGCTA120.158144438587243No Hit
TTCAACGGGGGAAAATGCCTACATCATAAACCTAAAGACTCTGGGACACAAA120.158144438587243No Hit
CATCAAGGGTGGCAAAGCCCACTGGGCAACTATCTCTAAATGCCAGGGGGCA120.158144438587243No Hit
ATCGCTGGGCAGTGTCAAATGGCGGTCTTATAGTTTCAATTCTGAAAATAGC120.158144438587243No Hit
CATGCCGGGAGCACAATTTGTCACTTTTATAAGCCCAGAAAATACCAAACTG120.158144438587243No Hit
CTAGCCGGGGCAAATGAGTGCAAATTTAAATGCAATGTTTTACTCACAATTT120.158144438587243No Hit
TTGCAAGGGATGTGGCATTCCTTTCTTTAAACTATAGGTAATTAAGGCAGCT120.158144438587243No Hit
TATGCAGGGCATCTGCAAGCCAAGGCAAGAAGCCTCAGAGGAAACCAGCCCT110.14496573537163943No Hit
GCGCCAGGGGTACAGGCTGCTGCCAATTTTAGAATTCAGTGGAAACTTTCTT110.14496573537163943No Hit
ATGCAGGGGCACTCCAACGCTGGGTGACATTGAGCTCACCAGCGCCACCGTC110.14496573537163943No Hit
TACGAGAGGGTATTTGTAAACTCTGTAATTAGGACCTTTTGCTATTTGAGTT110.14496573537163943No Hit
GCCTGAGGTTAAAAACAAAAAGTACGTGACCAGTCTGGTAAGAAGTATTAAT110.14496573537163943No Hit
GTCATGGGGGCATCGTGAGCTGCAGCAGTTGCACTCTTTTTCAGTGACCTAA110.14496573537163943No Hit
TAGTCTGGGATTCCAGCTTCTAATTAGTGTCAGTTCCTCTCAGATTCTGGCA110.14496573537163943No Hit
ATGACTGGGGGCACAAGATGGGAACTTAAACATCGTTTAGTCAGATGGTTGC110.14496573537163943No Hit
GAAGTAGGGTACTTTAGTTCCGATTCACCACTTTATCCTCCCTATCACCTTC110.14496573537163943No Hit
CTTCTAGGGAGAGTTCTTTTATTAAGATGTATCTGCTGGATTAAGGGTACAG110.14496573537163943No Hit
CAAGCAGGTTGGTACTACTTCTGATTAGGCAGAAAACACCAGGATTCCTTAA110.14496573537163943No Hit
TGTGCAAGGGTCTACAGCTGGGCAAATGACAGATAGACATTATCAACAGCTT110.14496573537163943No Hit
CTAGTGGGAACACACATAAAGTGGAAACAGAAACATAGTGTTAGGAGTATTT110.14496573537163943No Hit
GCAACTGGGATGATTAAAAACAGACTCTTCATCAAGAATAATGACTAATTCT110.14496573537163943No Hit
ATGCCAGGGAGGTTTTTTCCTGTGTTATCTTCTATGAGTTTTATAGTTTTGT100.13178703215603585No Hit
CCACCCGGGCCAGTGGTTGCCGTTCAGCACATGGGGATGCCGTCCTACCCTG100.13178703215603585No Hit
TCGCTGGGGGAACTTTAATCATATAAATTGTGGTTAATATAATGATCTATCA100.13178703215603585No Hit
CCAGCTGGGGTCCTCTGAATAGTCCTATCACTGTTTTCTGAGTTCAGACAAG100.13178703215603585No Hit
GAACAGGGACTAGCACCCAGGTCAATAAACAGATCATTACCAGAACCCCAGA100.13178703215603585No Hit
ACAGAGGGCTGTGACTCACACTGATATACCACTGTGTTCTAGCTTGGGCAAC100.13178703215603585No Hit
AGTCCTGGGTCTGTCAGGCCTGGGAGTGGAAGGAAGCAGAGCCTGAGGCCCT100.13178703215603585No Hit
CTGATGGGGAATCTTCTCAGGGTTTAAATCCACCCCTCTCTCCCCACAGAAA100.13178703215603585No Hit
AAGTTGGGGAAGAATAACAAATAGAAGGGAAGAGATAGGATGGGAAAGCTAA100.13178703215603585No Hit
GTCCATGGGAAGATAGCTTATCATATAGACTTGTTTGCCACCATTCCTGGGC100.13178703215603585No Hit
GGAAGTGGGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.13178703215603585No Hit
CTATGCGGGTCTCTAGAAGGGCATTGGGGTAATGGCAAAATAAAGTTGTATG100.13178703215603585No Hit
GCAGGCGGGATCACATAGTATTTCACATAATATCTATTGTTTACTTTCAGCA100.13178703215603585No Hit
CTGTTAGGGAGACTCTGTCTCAAAAATAGATAAATAAATAAATAAAAGTTTC100.13178703215603585No Hit
GAGATTGAAGAGCTAAACAGAGAGCTGGAAGAAATGAGGGTTACCTATGGGA100.13178703215603585No Hit
CGAACTGGGACTTTCCATCTAGCTCATGTTTTATCAAGAAACTGTTTGAGGA100.13178703215603585No Hit
TATCATGGGCTTCTCCTGTATTCCAGGGCAACTCCAACAACATTGCCACCGT100.13178703215603585No Hit
ATCGGCGGGAGCCTGTTCAGGTTTGATTGGAGGCCAATCTTTTACCAAGCAA100.13178703215603585No Hit
AAAAGCGGGGCAGTGGGAGAAGGCTAACCCCAAACATATGCCAAGCCAGGAG100.13178703215603585No Hit
CTCATGGGGAGCTCCGACTGCGGCGGCATCCGGGACGGCGGGCGGGCTGGCC100.13178703215603585No Hit
ACTTAGGGTGTCTAACACATGGTAAGGGTGCAGTAAATGTCAGTTGTAATAA100.13178703215603585No Hit
GGGCTGGGGGGTCACTTGACAAGAAAAAACCATACTTACCTGGGCCAAGGCT90.11860832894043227No Hit
TATTCGTGGGGTCTATTTATGATAACAGTACACGTGTTCTGCTTGAATTTAC90.11860832894043227No Hit
ATTTAAGGGCCTAGGTGCAATTAGGTTGTTTCTTTGTTTTTAGTTTCAATTC90.11860832894043227No Hit
TGAGATGGGGGCTGTGCATTTTAGAATGTATGATATCTGCCAAATCTGAGTG90.11860832894043227No Hit
CTACGGACGGGAATGAAATGAAGTGAAATGAAATGAAATAATGAAATTGAAA90.11860832894043227No Hit
TTCATGGGGACTCTCAGAAAGCAAAGCCCAGTAAAATATTAAACAAATAAAA90.11860832894043227No Hit
GGCGTGGGAGAAGAAGTCCGTGGACAAGGCTTACTTGGAACAGGAGCTCTGG90.11860832894043227No Hit
TGCACTAGGGATGTAGTAATTGAAAATATTATTTTTATTTCCTACATTTTTT90.11860832894043227No Hit
CAAAACGGGGGTTTCTCTCTTAAAGACACTCACTGTACAACTGAAAGCAGCT90.11860832894043227No Hit
TCAAACGGGGATCTAAACTCGGGATTCTATGAGAATACAGTTCAGACTTTGC90.11860832894043227No Hit
TGCGTGGGGTACACATTCTTCTTAGTGTAAATATGCCTTGAATTTGCACAAG90.11860832894043227No Hit
CTTCTGTAGGGTGGGTGAACTAAGAAGTCATCTTCAAACAGTATTTATAATT90.11860832894043227No Hit
ATAGTCGGGAACCTTCTCCCTTAAAGTTATCCCATGTCTCCTCGACGACGTC90.11860832894043227No Hit
ACTCACGGGAGGAATGGTCTTTATCATTTTTTTCCCCCATGAATGGGTCATA90.11860832894043227No Hit
TTCCTTGGGCTGGAGCAGGGCAATTGAAAAACTAAAATGTCTCATATGTTTG90.11860832894043227No Hit
TTGCAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC90.11860832894043227No Hit
TCAAAAGGGAAATATGAAGTGGATTGAAGCTAAAATGAAAGAGCTGGGAGTG90.11860832894043227No Hit
GCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCC90.11860832894043227No Hit
CTTAATGGGACAAATATGTTTAATTACTGTATATCTCTAGAAACATTTAAAA90.11860832894043227No Hit
AAAACAGGGGTACCACTTGTATTGCCAAACCTAAGTTTTCTGCTTTTAAATA90.11860832894043227No Hit
AGGGCTTGTCCTAGTCTTTAGATACTGAGCTCAAGTGTCCCTAAACTAAATT90.11860832894043227No Hit
TACGATGGGTGCTGGTGGTTTTATATTTAGATTCCAACTAGATTGTTATTCT90.11860832894043227No Hit
GCGCGGGGGGAACTCTTAAGGATTTCACCAGACCTGTTGGCTGCTGCTGTGA90.11860832894043227No Hit
TTTGCTGGGACAAGGTCATGGCCTTTGTCATAGCAGTTGAATGTGCATTGAC90.11860832894043227No Hit
TCGTCTGGGCACATGGTTCACTTTTGAAGTCTGCCAAACATTTAAAAGAAAT90.11860832894043227No Hit
TGGAGTGGGGGGGGCTGTTTAGAATACTTTTTTCTGAATTTCTAAACATCTG90.11860832894043227No Hit
GCTGTGGGGAACTGGGCATTGTAGAGTATCCTAAATATGTTATCAAGTATTT90.11860832894043227No Hit
TCCACAGGGCATGTTGACTTCCCTAGAGCAGGTGTCAGTTTTCATCAAGAGT90.11860832894043227No Hit
AAAGGCGGCTTCTCATTTGTTCTGATTTAACTCACTACAAATTCTACTTGCT90.11860832894043227No Hit
GCATGCAGAAACTCTGAGCTGGAAAAATTTGAGTCAGACTCTAATTCCTTGA90.11860832894043227No Hit
CAAATTGGGTAACTTATTTGAGAATATATTGTCTATGTTTTATCCTGTTTTA80.10542962572482868No Hit
CGTAAGGGTGGAATACTTCCTCAAGTCCTTTCCTGGTAATTATGTGATTCAC80.10542962572482868No Hit
ACATGTGGGGCAGGAGAAGCGGTGCCCACCAGCTTCCAGGTCTCCCTGACCT80.10542962572482868No Hit
CCGCATGGGGGGATTTTGGAGGAAAGGTTTAGAAGTTGGAAAAGATTTAAAA80.10542962572482868No Hit
ATACTAGGGATTACAAAAAACAAACAAACAAGCAAAAACACTTCAGTTCCAC80.10542962572482868No Hit
CGAAATGGGACCAACTACAAATGAGCACATCATTTTTTCAGTGTGGTTGACC80.10542962572482868No Hit
TTTGCAGGGATTCAGCAGGGCTGCCAAGCCCTCGACTAGTACCGAGGGTTGT80.10542962572482868No Hit
ACTGTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC80.10542962572482868No Hit
GACATAGGGATGTTTTGTGTACGTATGACAAACTGTCAAAGTGACACAGAAT80.10542962572482868No Hit
GGCATTGGGACTGGGGGAGTGGGAAAGTGATTCTGTAACCAACATTAGAATT80.10542962572482868No Hit
TAGGCGGGCTCCCTCAGGATATCTGAGAAGACATCGGACTGGCAAACGGTAA80.10542962572482868No Hit
GGTGGTGGGATAACACTGTAATTGTGGTGTATAAACTACTCAAGTACAAAGA80.10542962572482868No Hit
GCGACTGGCTTGGCTACTAACTCTAATTTATGCATTGGATATTTTACTACTT80.10542962572482868No Hit
GATACCGGGCTACCTTGAAAAACCACTGCGTCACCAAAGAACCGTCTCAGGT80.10542962572482868No Hit
GCACCAGGGACCAATAATCAGGTGAAGAAACCATGAACTAGAGGTAGCCAAA80.10542962572482868No Hit
ACGCTAGGGATATCAATACGTTAAAGCACTTTAGAATCAATACACTGATACA80.10542962572482868No Hit
CCACCTGGGAGTATATTTTTCATAAGACAAGAACATGGATCTAGTCCTGTGT80.10542962572482868No Hit
TGTTGGGGGATATTGAAGAATGTGAAAAAGAACCTGAAGTTAAGAAATTAAG80.10542962572482868No Hit
GGCAGGGACAGATGAAGTGCAGGTCTACCACAGCAACAGGTCTGAATGGGAA80.10542962572482868No Hit
CAACATGGGTAAGGTGTTTAACTACAGGTTCAATTACATATATATAAATATT80.10542962572482868No Hit
CGCTTTGGGCCTCATGCAACTTTCTGCAAAACTGAAGGTTGGATTTTAAATA80.10542962572482868No Hit
GAATAAGGGGGAGCAGAAAAAAATAAAGAATGGTAATTAAAGCAGCCACAGT80.10542962572482868No Hit
GCAAAAAAGTTTGTACTTCTAAGTCTGATCTGAGATCTAATGACTTCAGCAT80.10542962572482868No Hit
TATGTCGGGGTCCATCAACAGATGAATGCATAAAGAAGATGTACATATACGC80.10542962572482868No Hit
TACCAGGGGATGTCCACACATGCTCATAGCAGTGTTACTCACAATAGCCAAA80.10542962572482868No Hit
TTAGCAGGGAGTTAAGTAATTATTTAAATTATCAGTATATGGACAATATCCA80.10542962572482868No Hit
TGGTTAGGGATAGAACCAACGGAAATATGGGCACTATTTTGAATTCTAGAGA80.10542962572482868No Hit
CCTCCTGGGAGCTCTGTCAAGACTGGAACCTAGACTGTCAATTATAATGAAA80.10542962572482868No Hit
GCTGGCGGGAGGCGTGAGCAACCGCGCCCAGCCGAGATTGCCCCGTTTTTTT80.10542962572482868No Hit
TCCCATGGAAAAGGAAACAGAATAGTCAATGGAAAAGGAGAAGGACCTCCTG80.10542962572482868No Hit
CTTTCTGGGGTTTACAACATACACAAATGTGATTTAATGCTATTTAAATAAT80.10542962572482868No Hit
ATGCGAGGGGTTTCTTTTTGAAAAGAAAAGCTGAAATATTATTAAATGTTAG80.10542962572482868No Hit
CTTAGATGGGGATGAAAAGCAAAACAATCTAGTAAATACATTGTGTACCACT80.10542962572482868No Hit
ACTCGGGGAATATCCAAAATATATGAAGCACCTCTGCAAATTAATAAAAAAG80.10542962572482868No Hit
ATTGGTGGGCTAACAAGATTAAATATCCAGCCAGTACAGTATTTTTTAAGGC80.10542962572482868No Hit
TTTGTTGGGGTGTACAGACTTTTTTCTTGTCCTTATTCCTTACACAATATAG80.10542962572482868No Hit
TGTGGTGGGGTATCAGTGATGCCATGAGTTGGCTGAATAGTAAGATGAATGC80.10542962572482868No Hit
ACACCTGGGGGACTCCCAAAGTGCTAGGATTACAGACATGAGCCACTGCACC80.10542962572482868No Hit
TAGTGGGGGGGGTGGAGATTCCAATTGGCAGGAATTAGTCATGCTCACCTGC80.10542962572482868No Hit
TCTAATGGGGCAGAACTTTAAAAGTCGTTAAAAAATAAGACCATTGTGACAC80.10542962572482868No Hit
GGCTTTGGGCAACATGTTTTTGATATTTGATAATGAAAAAAATCTTTGCTTG80.10542962572482868No Hit
CTACGCGGGACCTCATCTTTGAATTTAGAATAAAAGTGGCACATTACATATC80.10542962572482868No Hit

[WARN]Adapter Content

Adapter graph