FastQCFastQC Report
Sat 31 Dec 2022
SRR5845209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7442
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGC380.5106154259607633No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGCCGTCT330.44342918570276807RNA PCR Primer, Index 42 (95% over 21bp)
GTAGGGGGGTTTGGGAAATCTTTTTGTAGCTTTTAAAGAGATGTGCGGGGAA170.22843321687718354No Hit
GGCAGCGGGCATGATACCCTGCCAGCTGTGAGGGGACCCCGTTTTTTAAGAT160.2149959688255845No Hit
TCAAACGGGGATCTAAACTCGGGATTCTATGAGAATACAGTTCAGACTTTGC160.2149959688255845No Hit
CATGCTGGGACCTGGTGTGGTCAGTATTTTTAATTTTAATTTTAGTCATTAT160.2149959688255845No Hit
TACATGTGGGGGAGCAGAGAGCAAGGATTTATTTTCAACTGAGAACCAAAAA150.20155872077398548No Hit
GCGCTTGGGATCTTGACATGGGACTACAACTTTTAGATCTTCATGATGCTAA150.20155872077398548No Hit
CTCACACCGGGATGTTCAGGAATCTTGGCTTTTTAAACCGGCTCTACCCCTA150.20155872077398548No Hit
GTCATAGGGCAAATTGAAAGGATAGCCAGGATGACTAAGACATACTCTTACA140.18812147272238647No Hit
TGAAAGGAAGTATGCTGTTCTCTATCAGCCTCTATTTGATAAGCGATTTGAA140.18812147272238647No Hit
TTACGAGGGCACGAGTGAGAAACAGGCCAGTCAGGAGGCATTTCCAGGCATT140.18812147272238647No Hit
CAGCTGGGGATGTGATGAATTACGTTTATTGATTTGCATATGTTGAACCAAC140.18812147272238647No Hit
TCAGCAAGGGCTGATTCATACAGTTCCAACCTTTTTCTAGTTTCACTTGCTG140.18812147272238647No Hit
ATTTAAGGGCCTAGGTGCAATTAGGTTGTTTCTTTGTTTTTAGTTTCAATTC130.17468422467078742No Hit
TTCATGGGGACTCTCAGAAAGCAAAGCCCAGTAAAATATTAAACAAATAAAA130.17468422467078742No Hit
AAGGCAGGGGGAGTCTGGCAGCAGACTTCTCAGTGGAAACTCTACAGGCCAG130.17468422467078742No Hit
CTATGCGGGTCTCTAGAAGGGCATTGGGGTAATGGCAAAATAAAGTTGTATG130.17468422467078742No Hit
AAAACAGGGGTACCACTTGTATTGCCAAACCTAAGTTTTCTGCTTTTAAATA130.17468422467078742No Hit
TTACTTGGGAGACATACCAAACTGAACTTTTCATTGGCACTTTTTAATGAAA130.17468422467078742No Hit
TATGTCGGGGTCCATCAACAGATGAATGCATAAAGAAGATGTACATATACGC130.17468422467078742No Hit
TGGTCAGGGACTAGTGGGAGACTTTAACATCCACCTGCCAATATTCAACTGA130.17468422467078742No Hit
ATATAAGGGGTCTCTAGTGTGATATATAAAGGTGTAATTAAGACAGAGTTCT130.17468422467078742No Hit
TACAATGGGTCATGTTGAATTGTAATACCCAGTGCTGGAGGTGGAGCCTGGT130.17468422467078742No Hit
ACAGAGGGCTGTGACTCACACTGATATACCACTGTGTTCTAGCTTGGGCAAC120.16124697661918838No Hit
GTTACTGGGAGATTATAGATGTTTTTGAAGAATACATTTTTTTGCATTAATA120.16124697661918838No Hit
TGCGTGGGGTACACATTCTTCTTAGTGTAAATATGCCTTGAATTTGCACAAG120.16124697661918838No Hit
GAGGCACGAGAATTGCTTGAACCCGCGGCGCGGAGATTGCAGTGGGCCGGGA120.16124697661918838No Hit
TTGGCAGGGGAATTTCATGAGCAGTGGGCAGCTGAATGGTGAAAGACCCAAA120.16124697661918838No Hit
GCAACTGGGATATTACAATCAAATATTGTAATCACTGACACAAGAATCTTAA120.16124697661918838No Hit
TGCTATGGACCAATGCATCTCTTTAAAGGCAAATATTATCCAGCAAGCAGTC120.16124697661918838No Hit
CCTCCTGGGAGCTCTGTCAAGACTGGAACCTAGACTGTCAATTATAATGAAA120.16124697661918838No Hit
TAAGCCCGGGATCTAGATCTGCTTTCTTTTATTACAAAAGAAAAAAGAGGGG120.16124697661918838No Hit
TGCTTTGGGAGCTCAGAAAACTATTTTTGTCTCCATCTTTTTTACACACAGT120.16124697661918838No Hit
ACGCATGGGGTTCTAGGGAAATAAAAGTCTAGCAGTTCAAAGTTTAAGTTTT120.16124697661918838No Hit
ATGCCAGGGAGGTTTTTTCCTGTGTTATCTTCTATGAGTTTTATAGTTTTGT110.14780972856758937No Hit
GCTTCTGGGCTGTTGCCTCTCAAAATCCGTTATGCTAAACTCACCAAATACG110.14780972856758937No Hit
CTGATGGGGAATCTTCTCAGGGTTTAAATCCACCCCTCTCTCCCCACAGAAA110.14780972856758937No Hit
GGCAGGGACAGATGAAGTGCAGGTCTACCACAGCAACAGGTCTGAATGGGAA110.14780972856758937No Hit
CCGTATGGGAGGGAAAGCCAAAGATTGGACACTCCTGATAAGCGCTCAGTCA110.14780972856758937No Hit
TACCAGGGGATGTCCACACATGCTCATAGCAGTGTTACTCACAATAGCCAAA110.14780972856758937No Hit
TACACAGGGACGGTAGGAGAAATTCAAAGCTTTGTTTTATTTGTGTTAAGTG110.14780972856758937No Hit
TGTTAGGGGACTCTTAAGAAGGGACGGGGCCCCACGCTGCGCACCCGCGGGT110.14780972856758937No Hit
GCATAAGGGGGCAGTATGGCCATTTTCATGATATTGATTCTTTCTATCCATG110.14780972856758937No Hit
GATACTGGGTACTTTGAAAGATGAAAAGTTAGTGCTGTTTTTGTCTACGTTA110.14780972856758937No Hit
GGCGTGGGAGAAGAAGTCCGTGGACAAGGCTTACTTGGAACAGGAGCTCTGG100.13437248051599032No Hit
ACATATGGGATATATATGATAGGCAAAACAAAAATCATTTTGTGACTAAAAC100.13437248051599032No Hit
GGCCCTAATCTATTTTTGTACTCCATTGCATAGATCATCTTCCAACATTATA100.13437248051599032No Hit
GGACACGGGGGCCGGAAATGATTTTGGAAAAGGACTTAATTATCTTTAACAA100.13437248051599032No Hit
AGTACTGGGTAAGAATTCCTTGTTTCAATGTGTTATTTACTAACATGAAGCT100.13437248051599032No Hit
ATGCAGGGGCACTCCAACGCTGGGTGACATTGAGCTCACCAGCGCCACCGTC100.13437248051599032No Hit
TTCAACGGGGGAAAATGCCTACATCATAAACCTAAAGACTCTGGGACACAAA100.13437248051599032No Hit
AACAAAGGGTAATTGGCATAGACATAAAGGATAAAAGGAAAATAACCGTCTG100.13437248051599032No Hit
ATGATTGGGAGCTCAGGGTTGTTTTGGAGCCTGTTGACTTTGTATCTCTGCC100.13437248051599032No Hit
GCACCAGGGACCAATAATCAGGTGAAGAAACCATGAACTAGAGGTAGCCAAA100.13437248051599032No Hit
GTGTGCGGGCTCCTTTCCTTGGAGGAGTCAGGGAACACTCTGCTCCACCAGC100.13437248051599032No Hit
CTTAATGGGACAAATATGTTTAATTACTGTATATCTCTAGAAACATTTAAAA100.13437248051599032No Hit
CACGACGGGCGCACTGGCCTTTGATGATTTCAATATAATCTGGAAAATGAAG100.13437248051599032No Hit
TGCACTGGGATACTTGCTATTGGCCATATGGAGCTCTTCAGCCAACTTTCCT100.13437248051599032No Hit
TTTTGTGGGGTCCTATGAATTTGGGAACTTCTCTCTTTGGTCATTGTCTTTT100.13437248051599032No Hit
CAAGCAGGTTGGTACTACTTCTGATTAGGCAGAAAACACCAGGATTCCTTAA100.13437248051599032No Hit
GTAAATGGGTCAATATACTATTTTATAATTATGAAGTGGAATGAATTTTAGA100.13437248051599032No Hit
CCGGGTGGGCTCTGTCCCCTCATGACTTTTTAGACACAGGGACTAGGACATC100.13437248051599032No Hit
CATGCCGGGAGCACAATTTGTCACTTTTATAAGCCCAGAAAATACCAAACTG100.13437248051599032No Hit
AATGAAGGAGAAAGCTCAGCTTGAAAAAACAATAGAAACACTGAGAGAAAAT100.13437248051599032No Hit
CGTTGCGGGCATAAGTCTCAATTTCCCTGTCTGTAATTAGATCCCACTTTAT100.13437248051599032No Hit
GTAGACGGGTGTATTTAATTAGGTTCCTTATTCAGTTATTCAAGTATCCAGT100.13437248051599032No Hit
TATCATGGGCTTCTCCTGTATTCCAGGGCAACTCCAACAACATTGCCACCGT100.13437248051599032No Hit
TGCACAGGGTGAAAGAATTATCTAAACGTATATTTGTCAGATCAATACCGTC100.13437248051599032No Hit
TCTCGTGGGGCAACTCGTTAGCAGAGGAAAACAGCATGTGGGTATTTGTCGA90.1209352324643913No Hit
TATTGTGGGATGTAATAGAATGTTAGGTAGGACCTAACATTATATTATGCTT90.1209352324643913No Hit
AACCGGGGGATTAACAGCACAAGTGGTAGGTTAACATTAGAATAGGAATTGG90.1209352324643913No Hit
AGAAATGGGACAATCGGAGTTGCAGCCAAAAATCAGCAAATCACTCATGCAA90.1209352324643913No Hit
GTAAGAGGGGCAGTATCATAAGAACTGGAAAAACTCAAATCCGCCAAAAATC90.1209352324643913No Hit
TGTCCGGGCAAACTTTAGGGCAGGCTGTTTGACCAGGAGCCTCAGTTCCTTG90.1209352324643913No Hit
ACGCATGGGGTGGCTTTATTTCTGAGTTTTCTATTCTTTTCCATTGGTCTAT90.1209352324643913No Hit
CGAAATGGGACCAACTACAAATGAGCACATCATTTTTTCAGTGTGGTTGACC90.1209352324643913No Hit
ATGGCCGGGAGCAGACATGTATTTCTTGCACAGCTAATAGAAGACAAAGTTG90.1209352324643913No Hit
GCGCAAGGGAGATCACCCCGTTCCTGATATTTATTATTGCAAACATTCCACT90.1209352324643913No Hit
GCCTGAGGTTAAAAACAAAAAGTACGTGACCAGTCTGGTAAGAAGTATTAAT90.1209352324643913No Hit
GCGACTGGCTTGGCTACTAACTCTAATTTATGCATTGGATATTTTACTACTT90.1209352324643913No Hit
ATAATAGGGGAAGCAAATACAGTTTGGACTGAGGCATCACAATCTGCAGAAA90.1209352324643913No Hit
ATAGTCGGGAACCTTCTCCCTTAAAGTTATCCCATGTCTCCTCGACGACGTC90.1209352324643913No Hit
TACTATGGGACTTTAATAATCCAGATGCAATCATTGGAAGTATTTAATATGC90.1209352324643913No Hit
ACTTGCGGGGCTCTCAGAATCGCTGCCGCCATGGCTAGTCAGTCTCAGGGGA90.1209352324643913No Hit
CTCACCGGGATTGCTCACCTTTTTTGCTTACCATATTCCAAGAATGTGTCTA90.1209352324643913No Hit
ACGCTAGGGATATCAATACGTTAAAGCACTTTAGAATCAATACACTGATACA90.1209352324643913No Hit
GTCATGGGGGCATCGTGAGCTGCAGCAGTTGCACTCTTTTTCAGTGACCTAA90.1209352324643913No Hit
AAACCAGGGAGAGGCAGGCTTATACCAGATCATGAGACTCTTTAAAGATTAT90.1209352324643913No Hit
TAGTCTGGGATTCCAGCTTCTAATTAGTGTCAGTTCCTCTCAGATTCTGGCA90.1209352324643913No Hit
GCACATGGGGAACCTCTGAAAAGAATATATTCTTCTTTGTAGTCCTTCTTCC90.1209352324643913No Hit
TTTACAGGGGGTAGAGCTGGTCTCTGAATTTCCAAGCATTGGCATATAAAAT90.1209352324643913No Hit
TGCAGTGAGCCAAGATTGGGCCAGTGCACTTCAGCCTGGGTGACAGAGAGAG90.1209352324643913No Hit
CTTCTTGGGGCTGTGGGAACAGACCAAGAACGTTAATTACTCAGGCAGGGTC90.1209352324643913No Hit
ACTCGGGGAATATCCAAAATATATGAAGCACCTCTGCAAATTAATAAAAAAG90.1209352324643913No Hit
GCTTTGGGGAATGTATTTGAAAAAATTCTCACTTCTGCAAGGCCAGTTTTCA90.1209352324643913No Hit
TCTAATGGGGCAGAACTTTAAAAGTCGTTAAAAAATAAGACCATTGTGACAC90.1209352324643913No Hit
CTCAGGGGGATGAGCGGAGTCTGTCCTCTGCTCTTCTGCAGTCAGGTCACTG90.1209352324643913No Hit
CATTGTGGGGATGTTTGTACACCCATGTTTATAATGTGATTATTCACAATAG90.1209352324643913No Hit
AAAAGCGGGGCAGTGGGAGAAGGCTAACCCCAAACATATGCCAAGCCAGGAG90.1209352324643913No Hit
CTCATGGGGAGCTCCGACTGCGGCGGCATCCGGGACGGCGGGCGGGCTGGCC90.1209352324643913No Hit
TCGTTTGGGGAAGTTGGAGACCACCTGACTGAGGCTAGTGGGAAGCTTCAGC90.1209352324643913No Hit
CTGTCCGGGAGGAACAGAGGCACTTTAACAAGTTTAGAACAAAGCTTTTCAA90.1209352324643913No Hit
AGCGCTGGGACCACAATATTACAACAGCCTCCTTGGAGTGAGGCCCACCACA90.1209352324643913No Hit
CAGCAAGGGAGAAACAATCATATATAAAGAGAAACTCTGTAAGTGGAATCAT80.10749798441279225No Hit
CCACCCGGGCCAGTGGTTGCCGTTCAGCACATGGGGATGCCGTCCTACCCTG80.10749798441279225No Hit
TCACTTGGGGGAACACACACAAAAAAATCTGTCAGCTTACAAAAGCACAACA80.10749798441279225No Hit
GAACAGGGACTAGCACCCAGGTCAATAAACAGATCATTACCAGAACCCCAGA80.10749798441279225No Hit
AGCTCTGGGATGGATCTTTTCACTGGCTGACTCTCCCATATCTGCAAGAGAT80.10749798441279225No Hit
TCCAAGGGGACTACTTTGAAACATAAGTTAAAGCCATTAGCTGTGTTTTTGT80.10749798441279225No Hit
ATAGGCGGACACCAATAATTTTCAAATCAAGAACAAAATCAAGAACCCAATT80.10749798441279225No Hit
TGCACTAGGGATGTAGTAATTGAAAATATTATTTTTATTTCCTACATTTTTT80.10749798441279225No Hit
TAGTAGGGGATTGTCCCAAAGTCTAGAGTCTTTACAGGTAGGCAAAATTTGT80.10749798441279225No Hit
CCTTAAGGGAGGCAGAAACAATCTATAACAGAAACTTGGATCCTCAAAAAGA80.10749798441279225No Hit
GCTTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC80.10749798441279225No Hit
TAAGCGGGGAGGCTCAGATTTACCTGGCTTCTGTTTACCTGGCTTCTGCAGA80.10749798441279225No Hit
CCCACTGGGAAAGTGAGGAGCAGGAAAAACAGAGAAGGAGCCTGGCGCTCTG80.10749798441279225No Hit
GATGCAGGGATTTCAGAAATTTGAATGAAAAGTAGTATTCTTCCCATATATT80.10749798441279225No Hit
CACTGTGGGGACCTTTCCGAGTTGGCTGCAGATTTGTGGTGCGTTCTGAGCC80.10749798441279225No Hit
CAGCCTGGGCAACAGAGCGAGATTCTGTCTCGAAACAAACAAACAAAAAACG80.10749798441279225No Hit
TCAAAAGGGAAATATGAAGTGGATTGAAGCTAAAATGAAAGAGCTGGGAGTG80.10749798441279225No Hit
ACACTTGGGATGTTCATACCAGTTAAACTGCACTTTCCCAAGGGGACAGCTG80.10749798441279225No Hit
GCAGGCGGGATCACATAGTATTTCACATAATATCTATTGTTTACTTTCAGCA80.10749798441279225No Hit
CTATTAGCGGGCTGTAAAAGCTATATAATTGATATGATTAACTATTTAGATA80.10749798441279225No Hit
CCATAGGGACCAGAGAGGGTGGGGACCAGTTTTATCCATCCCTCAAAAGCTC80.10749798441279225No Hit
GCTACAGGGAATCTGTTACTAGTACCATCTTGACAGAGGATACATGCTCCCA80.10749798441279225No Hit
TAGCCTGGGAAGGACAGCCACTTTGAGTTCCAAAACTGAAGAACTTGGAGTC80.10749798441279225No Hit
GACGATGGGGCTTTGTTTGCATGTCCACTAATTTTTTTATTTTGATATTAGT80.10749798441279225No Hit
ACAAATGGGATGTGATGTCTGGAGCATAGGATGCATTCTTATTGAATACTAT80.10749798441279225No Hit
AGGGCTTGTCCTAGTCTTTAGATACTGAGCTCAAGTGTCCCTAAACTAAATT80.10749798441279225No Hit
TCAATGGGGGTTCCAGTTGGGATGAAGCATTTCATCTCACTTCATAACACCT80.10749798441279225No Hit
TGTGCAAGGGTCTACAGCTGGGCAAATGACAGATAGACATTATCAACAGCTT80.10749798441279225No Hit
GAACCCGGGCACTCTTATAAGGGTATAAAAATTCAACTACATTTCATGCTCA80.10749798441279225No Hit
AGCCTGGGCATTTATGGAAAGTTGCTGTTATGGGAATAATAGTTCTGAACAG80.10749798441279225No Hit
ACGGTGGGATTCACGCAGCCGTCCTTATCCTGTGTGCTTTAGGCCTTGAATT80.10749798441279225No Hit
TCAATCGGGCCGATCTCTACTAGGGAAATAAAGTAAATGCTTGTTTCATTTT80.10749798441279225No Hit
GCCAATGGGCATAAGTTGAAATTATATTTTAACTGAAAAGATATCATTAAAG80.10749798441279225No Hit
ATGCGAGGGGTTTCTTTTTGAAAAGAAAAGCTGAAATATTATTAAATGTTAG80.10749798441279225No Hit
GGCTGGGGGGGTTCAAGTGCTGCAATCTGTATGTACTAAATAGCTTTACTTT80.10749798441279225No Hit
TCGGCTGGGGAGAGGAGAGGCATCTATACTTACAGCATACAGAAAAGATTTT80.10749798441279225No Hit
GCACTGGGGTTTTGAGATGGAATCTCGCTCTCTCGCCAGACTGGAGTGCTCC80.10749798441279225No Hit
GGCTATGGGTCCTTTCAGCATCTATATTGGAAAGCGACTGAGCACACCCTCT80.10749798441279225No Hit
AAACCTGGGAGCCCAGGTCCAGAGCCAAACCCGTCACTGACCCCCCAGCCCA80.10749798441279225No Hit
CTACGCGGGACCTCATCTTTGAATTTAGAATAAAAGTGGCACATTACATATC80.10749798441279225No Hit
CTAGCCGGGGCAAATGAGTGCAAATTTAAATGCAATGTTTTACTCACAATTT80.10749798441279225No Hit
ACCTTGGGGTAAGTATAACATTGCGATTTAAAACAATAAACCAGATTGAGAT80.10749798441279225No Hit

[WARN]Adapter Content

Adapter graph