FastQCFastQC Report
Sat 31 Dec 2022
SRR5845208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7470
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGC500.6693440428380187No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCAAGCGATCTCGTATGCCGTCT400.535475234270415RNA PCR Primer, Index 42 (95% over 21bp)
CATTGTGGGGATGTTTGTACACCCATGTTTATAATGTGATTATTCACAATAG200.2677376171352075No Hit
CGAACTGGGACTTTCCATCTAGCTCATGTTTTATCAAGAAACTGTTTGAGGA160.214190093708166No Hit
TGCGTGGGGTACACATTCTTCTTAGTGTAAATATGCCTTGAATTTGCACAAG150.2008032128514056No Hit
AAGACCCCTGTCAGTTTTATTGCTCTGACCTTGACGGTGAGGGTTTCCTGCA150.2008032128514056No Hit
TTTTGTGGGGTCCTATGAATTTGGGAACTTCTCTCTTTGGTCATTGTCTTTT150.2008032128514056No Hit
ACTCGGGGAATATCCAAAATATATGAAGCACCTCTGCAAATTAATAAAAAAG150.2008032128514056No Hit
TCGCTGGGGGAACTTTAATCATATAAATTGTGGTTAATATAATGATCTATCA140.18741633199464525No Hit
ATAATAGGGGAAGCAAATACAGTTTGGACTGAGGCATCACAATCTGCAGAAA140.18741633199464525No Hit
GGAAGTGGGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.18741633199464525No Hit
AGGGCTTGTCCTAGTCTTTAGATACTGAGCTCAAGTGTCCCTAAACTAAATT140.18741633199464525No Hit
TGGTTAGGGATAGAACCAACGGAAATATGGGCACTATTTTGAATTCTAGAGA140.18741633199464525No Hit
GCGCAACGGGGAGAGAGCTTCAAAGAGGAGGAGAGGGCTATGATTGAGGTGT130.17402945113788487No Hit
TGCTATGGACCAATGCATCTCTTTAAAGGCAAATATTATCCAGCAAGCAGTC130.17402945113788487No Hit
TAGTACGGGTCCCACTGCTGGTTCTGCATCTCAATGCCTATTCTAGGAGAGG130.17402945113788487No Hit
CTCAGGGGGATGAGCGGAGTCTGTCCTCTGCTCTTCTGCAGTCAGGTCACTG130.17402945113788487No Hit
ATACTAGGGATTACAAAAAACAAACAAACAAGCAAAAACACTTCAGTTCCAC120.1606425702811245No Hit
AGAAATGGGACAATCGGAGTTGCAGCCAAAAATCAGCAAATCACTCATGCAA120.1606425702811245No Hit
TGTCCGGGCAAACTTTAGGGCAGGCTGTTTGACCAGGAGCCTCAGTTCCTTG120.1606425702811245No Hit
ACGCATGGGGTGGCTTTATTTCTGAGTTTTCTATTCTTTTCCATTGGTCTAT120.1606425702811245No Hit
GGCGCTGGGCTTGGCAAGTTGCAGAGATGGTGGAGCTAGAAAAAAAAAAAAA120.1606425702811245No Hit
ACTTGCGGGGCTCTCAGAATCGCTGCCGCCATGGCTAGTCAGTCTCAGGGGA120.1606425702811245No Hit
ACTTGAGGGTAAGTGATGTTGATTGATAAGGTGAATATTTACTATTGTTTGG120.1606425702811245No Hit
CAACATGGGTAAGGTGTTTAACTACAGGTTCAATTACATATATATAAATATT120.1606425702811245No Hit
CGCTTTGGGCCTCATGCAACTTTCTGCAAAACTGAAGGTTGGATTTTAAATA120.1606425702811245No Hit
ATGACTGGGGGCACAAGATGGGAACTTAAACATCGTTTAGTCAGATGGTTGC120.1606425702811245No Hit
CTGTGTTTACATTCCCTGGCCTGCCCTTTCATGGGCCGTTCACGGGCCTGGC120.1606425702811245No Hit
GTCATAGGGCAAATTGAAAGGATAGCCAGGATGACTAAGACATACTCTTACA110.14725568942436412No Hit
TCTCGTGGGGCAACTCGTTAGCAGAGGAAAACAGCATGTGGGTATTTGTCGA110.14725568942436412No Hit
GCCTTGGGTATGTTGCTTTAAGGTATCTTTAAACAAGTAACTATTTTCTCTT110.14725568942436412No Hit
TTACAAGGGCACTTGGAAAAATACCTTAAAGCTCAATCATTGGATTGTATAA110.14725568942436412No Hit
CAAAACGGGGGTTTCTCTCTTAAAGACACTCACTGTACAACTGAAAGCAGCT110.14725568942436412No Hit
GCTAATGGGATTTCAATAAAATTTGCAGATTCACACCTGAGGATTTACTCTT110.14725568942436412No Hit
GCCTGAGGTTAAAAACAAAAAGTACGTGACCAGTCTGGTAAGAAGTATTAAT110.14725568942436412No Hit
TCAAACGGGGATCTAAACTCGGGATTCTATGAGAATACAGTTCAGACTTTGC110.14725568942436412No Hit
CCCACTGGGAAAGTGAGGAGCAGGAAAAACAGAGAAGGAGCCTGGCGCTCTG110.14725568942436412No Hit
GCAACGGGATTGTTTCTGCCCCACATCTTGATTTAACAGATTTTTAAGATGG110.14725568942436412No Hit
GATTCAGGGGATCTATTCACGGGTCGATCTATTGCCGGGCTGCTTCACATGT110.14725568942436412No Hit
CCTTCAGGTTACGTGAGCAACAGGTATAAGCTAAGCTTTAGAGTAATATACA110.14725568942436412No Hit
TATAGCGGGTGGTTGAAGTATTGTGTATTAAATTAAATTTGGTGGTTGAGCT110.14725568942436412No Hit
CCTCCTGGGAGCTCTGTCAAGACTGGAACCTAGACTGTCAATTATAATGAAA110.14725568942436412No Hit
ACGGTGGGATTCACGCAGCCGTCCTTATCCTGTGTGCTTTAGGCCTTGAATT110.14725568942436412No Hit
TGTTGTGGGAGAATGAGTTATGAAGTGTTCATTTCTTTATTTTCCTTCTTAC110.14725568942436412No Hit
ATATAAGGGGTCTCTAGTGTGATATATAAAGGTGTAATTAAGACAGAGTTCT110.14725568942436412No Hit
TGCTTTGGGAGCTCAGAAAACTATTTTTGTCTCCATCTTTTTTACACACAGT110.14725568942436412No Hit
ATTTTAGGGAGTTTTTGCTCTCACTAAACTCCTTTTTCCTTACAACTCTGAT100.13386880856760375No Hit
TATTCGTGGGGTCTATTTATGATAACAGTACACGTGTTCTGCTTGAATTTAC100.13386880856760375No Hit
GCCCATCTCTGCCTCCAAAACTGCTAGGATTATAGGCATGAGCCACCGCACC100.13386880856760375No Hit
GGCGTGGGAGAAGAAGTCCGTGGACAAGGCTTACTTGGAACAGGAGCTCTGG100.13386880856760375No Hit
TCTGTCGGGACTATGGGTCAGATGTACCCTGCTGAATAATAACTATCTATTA100.13386880856760375No Hit
ACTATAGGGTGTGTGAGCCAGAGGGAGCTGTGGTAAGGGTTGGGCCCCCAGC100.13386880856760375No Hit
AGCGGTGGGACAAGTAATACATTTTTTCTTCAAAGAGTAGTTTCTTATAAAA100.13386880856760375No Hit
GCCAATGGGGACTAGCTAAATATGATATTCTTAAAAAACAAGATATGATTTT100.13386880856760375No Hit
AAGCGAGGGCTGTTACAAAGCTCTCCCTCTCATGCAAAGTAGGGAACACCTT100.13386880856760375No Hit
GCACAGGGAAGCTTTTACAGATTTTACCTGGAAAAACAAAGTGGTTTATATG100.13386880856760375No Hit
TAGCCTGGGTGACAAGAACGAAACTCCATCTCAAAAAATAAAATAAAATATA100.13386880856760375No Hit
TGCAGTGAGCCAAGATTGGGCCAGTGCACTTCAGCCTGGGTGACAGAGAGAG100.13386880856760375No Hit
ATCGCTGGGCAGTGTCAAATGGCGGTCTTATAGTTTCAATTCTGAAAATAGC100.13386880856760375No Hit
CCTAGAGGGCAGGTATGCCACTTATTTTATTCATTTTTGTGTAAGGGAAATG100.13386880856760375No Hit
GTAGACGGGTGTATTTAATTAGGTTCCTTATTCAGTTATTCAAGTATCCAGT100.13386880856760375No Hit
TGTGGTGGGGTATCAGTGATGCCATGAGTTGGCTGAATAGTAAGATGAATGC100.13386880856760375No Hit
GCACTGGGGTTTTGAGATGGAATCTCGCTCTCTCGCCAGACTGGAGTGCTCC100.13386880856760375No Hit
GCATGCAGAAACTCTGAGCTGGAAAAATTTGAGTCAGACTCTAATTCCTTGA100.13386880856760375No Hit
TTGTTGGGGGATTGGGATGAGTGATTTTATAAGATAATATGGTGATAATTTT100.13386880856760375No Hit
GATACTGGGTACTTTGAAAGATGAAAAGTTAGTGCTGTTTTTGTCTACGTTA100.13386880856760375No Hit
CAAATTGGGTAACTTATTTGAGAATATATTGTCTATGTTTTATCCTGTTTTA90.12048192771084339No Hit
GTAGGGGGGTTTGGGAAATCTTTTTGTAGCTTTTAAAGAGATGTGCGGGGAA90.12048192771084339No Hit
CCAGCTGGGGTCCTCTGAATAGTCCTATCACTGTTTTCTGAGTTCAGACAAG90.12048192771084339No Hit
CAGCCTGGGAAACAGAGCGAGACTCCATCTCAAAAACAAACAAACAAAAAAA90.12048192771084339No Hit
TATTGTGGGATGTAATAGAATGTTAGGTAGGACCTAACATTATATTATGCTT90.12048192771084339No Hit
CTACGGACGGGAATGAAATGAAGTGAAATGAAATGAAATAATGAAATTGAAA90.12048192771084339No Hit
AAGGCAGGGGGAGTCTGGCAGCAGACTTCTCAGTGGAAACTCTACAGGCCAG90.12048192771084339No Hit
GACTAGCGGGGTACTTTTTATCTCTGACGTATCCCTTTTTACTTAAAAAATG90.12048192771084339No Hit
ATAGGCGGACACCAATAATTTTCAAATCAAGAACAAAATCAAGAACCCAATT90.12048192771084339No Hit
TTGCTTGGGATAAATCATTCTTTTTTTCCAATATACCTTAGAAAATGAAAAA90.12048192771084339No Hit
GCTTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC90.12048192771084339No Hit
CAGATTGGGGAACTGAGATCTACCTGTATTCTCATCTTGACCACGGTCATCT90.12048192771084339No Hit
GCATAGCGGGAAATGACTTTTGACATACAAACTCTTCTTGAGAATGTTTGTT90.12048192771084339No Hit
AAATAAGGGACACACACAAACCCCACTGGGATTCCAGTTAGCACGCACACAC90.12048192771084339No Hit
TTGGCAGGGGAATTTCATGAGCAGTGGGCAGCTGAATGGTGAAAGACCCAAA90.12048192771084339No Hit
ACGCTAGGGATATCAATACGTTAAAGCACTTTAGAATCAATACACTGATACA90.12048192771084339No Hit
GCAACTGGGATATTACAATCAAATATTGTAATCACTGACACAAGAATCTTAA90.12048192771084339No Hit
TAGCCTGGGTGACAGAGTGAGACCCCATCTCTTAAGAAAAGAAAATTGATTT90.12048192771084339No Hit
CAACATGGGGAAGGTGAGGGAATGGCACCATGAGTCAAGGAATGCAGGTGTG90.12048192771084339No Hit
TTACTTGGGAGACATACCAAACTGAACTTTTCATTGGCACTTTTTAATGAAA90.12048192771084339No Hit
GCAAAAAAGTTTGTACTTCTAAGTCTGATCTGAGATCTAATGACTTCAGCAT90.12048192771084339No Hit
ACAAATGGGATGTGATGTCTGGAGCATAGGATGCATTCTTATTGAATACTAT90.12048192771084339No Hit
GGCAGTGGGCTTCCGATCTTCTTGCGGGCAAGCGCCAGAAGTATGAACGGAT90.12048192771084339No Hit
TGTGCAAGGGTCTACAGCTGGGCAAATGACAGATAGACATTATCAACAGCTT90.12048192771084339No Hit
TTGTACGGGACAGTTTTGCCTTAAAGGACCTTCCCAAGTCTCCCTTTCCCTG90.12048192771084339No Hit
TTTACAGGGGGTAGAGCTGGTCTCTGAATTTCCAAGCATTGGCATATAAAAT90.12048192771084339No Hit
GCCAATGGGCATAAGTTGAAATTATATTTTAACTGAAAAGATATCATTAAAG90.12048192771084339No Hit
TCGTCTGGGCACATGGTTCACTTTTGAAGTCTGCCAAACATTTAAAAGAAAT90.12048192771084339No Hit
GCTGTGGGGAACTGGGCATTGTAGAGTATCCTAAATATGTTATCAAGTATTT90.12048192771084339No Hit
TCATTAGGGACCACCTCCTGGGTCCATCCTGTGACACATGGAATTCTGGGAG90.12048192771084339No Hit
TCCACAGGGCATGTTGACTTCCCTAGAGCAGGTGTCAGTTTTCATCAAGAGT90.12048192771084339No Hit
GTTCATGGGGTTCAGCTGAGTAACAGCGACCTTGCCTTTAGGTTATGTTTTC90.12048192771084339No Hit
CTACGCGGGACCTCATCTTTGAATTTAGAATAAAAGTGGCACATTACATATC90.12048192771084339No Hit
CTAGCCGGGGCAAATGAGTGCAAATTTAAATGCAATGTTTTACTCACAATTT90.12048192771084339No Hit
CAAAACGGGATGGAACAGGGTGGGACACATATAGTAGAGGCAGGCATCCAGT80.107095046854083No Hit
ATGCCAGGGAGGTTTTTTCCTGTGTTATCTTCTATGAGTTTTATAGTTTTGT80.107095046854083No Hit
TTTAAGGGATGCTATCCTGTATTAAAGATGTTAGATTTTTGAAAGGGGTGTT80.107095046854083No Hit
GCTTCTGGGCTGTTGCCTCTCAAAATCCGTTATGCTAAACTCACCAAATACG80.107095046854083No Hit
CGGGTAAAGAGACTTTTTAAAAAGATATTAATATATACGGACAAAAAGAAGG80.107095046854083No Hit
TTTGCAGGGATTCAGCAGGGCTGCCAAGCCCTCGACTAGTACCGAGGGTTGT80.107095046854083No Hit
GTTACTGGGAGATTATAGATGTTTTTGAAGAATACATTTTTTTGCATTAATA80.107095046854083No Hit
TAGCTCGGGACCTTTAAATGGGGGTAAGTGTGTTTACCTTGATGGGTTAATG80.107095046854083No Hit
CACTTGGGGAGGCTAAGAAAAAAAGAAGGAAGACACAAATATTAGTATCAAA80.107095046854083No Hit
TTTCCAGGGCTTCAAGGAAGAGCAAGGCACAGTAAGGCAAATAAATCCTTCC80.107095046854083No Hit
TTACGAGGGCACGAGTGAGAAACAGGCCAGTCAGGAGGCATTTCCAGGCATT80.107095046854083No Hit
CTCTCGCTGGGAGTCTATATAAGTGATGTATGAGTTGAGTTATGTAAATTTT80.107095046854083No Hit
GTCCATGGGAAGATAGCTTATCATATAGACTTGTTTGCCACCATTCCTGGGC80.107095046854083No Hit
ATGATTGGGAGCTCAGGGTTGTTTTGGAGCCTGTTGACTTTGTATCTCTGCC80.107095046854083No Hit
CATATGGGGAATCACATGGCTTTTGATACCCAGACTCATTATGTCAACCATC80.107095046854083No Hit
GACTATGGGTGACAGCTGGTCTTTAAACAAATTGCTTGGACTGAAGTGATAC80.107095046854083No Hit
ACACTTGGGATGTTCATACCAGTTAAACTGCACTTTCCCAAGGGGACAGCTG80.107095046854083No Hit
CCACCTGGGAGTATATTTTTCATAAGACAAGAACATGGATCTAGTCCTGTGT80.107095046854083No Hit
GTACCCTGGGAAAGTGGTTTTGTAGTGTTGGGTGCTATGAGTAGGTATGGAT80.107095046854083No Hit
GCAGGGGGGCCAAAAATTTTGTGCACAGTGATATGACTGTTTTAGGATCTTA80.107095046854083No Hit
TCAGCAAGGGCTGATTCATACAGTTCCAACCTTTTTCTAGTTTCACTTGCTG80.107095046854083No Hit
TAAAGTGGGTAATGACAGAGGAGCAGCACTATTTCAAAAGAACATGAATTAG80.107095046854083No Hit
TCCACAGGGACTGTGTGTTTGTTTGCTACATTTATATATAAAAAACATCCTC80.107095046854083No Hit
GCACTAGGGTATTGTGTTCCTATATGTTGAATTTTACTGTGTTTTAGTGAAG80.107095046854083No Hit
AGACTTGGGATCAAAATCCACTTAAAATGAAATTCCATTGAATATCTTAAGC80.107095046854083No Hit
GGAGCCTTGTGATACTGAATATCCAGCATTCGTCTCTGAGCGCACCATCAAG80.107095046854083No Hit
ACTAGTGGGCCTGGTCATTAATTATAACAAATGTGCCATACTAATCTAAGAT80.107095046854083No Hit
TATGTCGGGGTCCATCAACAGATGAATGCATAAAGAAGATGTACATATACGC80.107095046854083No Hit
CTTCTAGGGAGAGTTCTTTTATTAAGATGTATCTGCTGGATTAAGGGTACAG80.107095046854083No Hit
CTTCATGGGATACTCTGGGGAGTTCAGAGAAATTGGAACAGTGATTGATCAG80.107095046854083No Hit
ATGCATGGGAGTGCTGAAATCTGCAGCATGGTACTAAGGAAGTTAAAGTTTG80.107095046854083No Hit
GGCCATGGGATAATGTGAAATAATTTCAGCTAGTCTGAATATGGTTTTGTCC80.107095046854083No Hit
GTGTCCGGGCAAATAACTCACCTACAGATTTTTAGCACAAAAATAGCCATTG80.107095046854083No Hit
TAGCGCAGGGCAGCATTGAGAAGCCAATTCACCAGGGACCAGATGCTGCTGT80.107095046854083No Hit
CTAGTGGGAACACACATAAAGTGGAAACAGAAACATAGTGTTAGGAGTATTT80.107095046854083No Hit
GCTGGCGGGAGGCGTGAGCAACCGCGCCCAGCCGAGATTGCCCCGTTTTTTT80.107095046854083No Hit
ACACCCAGGCCATATTTCTGATATCTCTAAGGACTGTAGTAATTTGTATTTT80.107095046854083No Hit
CTTTCTGGGGTTTACAACATACACAAATGTGATTTAATGCTATTTAAATAAT80.107095046854083No Hit
TGTCCTGGGATGAGAGGTTATTGTAGATGTGTCCTGTGATCTGTAAGGCCGT80.107095046854083No Hit
GGTCATGGGCATAAAAACAGACACATAAATCAATGGAACAGATTAGAGAACC80.107095046854083No Hit
GTCAAAGGGAATCCTTCATAGCAAGAAATTAGGGAAGAGGGAATAAAATTCG80.107095046854083No Hit
CATGCCGGGAGCACAATTTGTCACTTTTATAAGCCCAGAAAATACCAAACTG80.107095046854083No Hit
TGCAAGGGGAAGCCTTTTGCAGCTCTGTGGCTTGGAATTTCATTTGAGCAAT80.107095046854083No Hit
ACACATGGGGCTGTTTTAAAACATTAGAATTTTGATCTTCAACCATAGTTTG80.107095046854083No Hit
TTTGTTGGGGTGTACAGACTTTTTTCTTGTCCTTATTCCTTACACAATATAG80.107095046854083No Hit
ACACCTGGGGGACTCCCAAAGTGCTAGGATTACAGACATGAGCCACTGCACC80.107095046854083No Hit
TTCACGGGTATCTGTCTAACTGTGACAGCTCTCTCCTATCTCCTTCTAGAAA80.107095046854083No Hit
CTAATGGGTGCACCCTGATATACAGCACGGAGCTGAAGGCTTTGCAGGCCAC80.107095046854083No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCAAGCG80.107095046854083No Hit
CTCATGGGGAGCTCCGACTGCGGCGGCATCCGGGACGGCGGGCGGGCTGGCC80.107095046854083No Hit
CTGTCCGGGAGGAACAGAGGCACTTTAACAAGTTTAGAACAAAGCTTTTCAA80.107095046854083No Hit
AGCGCTGGGACCACAATATTACAACAGCCTCCTTGGAGTGAGGCCCACCACA80.107095046854083No Hit

[OK]Adapter Content

Adapter graph