FastQCFastQC Report
Sat 31 Dec 2022
SRR5845185.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845185.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200082
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT10320.5157885267040513No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC9850.4922981577553203No Hit
CCTAGGGGGAAATCCGTCCTTGCAGAAACAGAAGGAATTTTACAGAAGCTAC3100.15493647604482164No Hit
GCACCCAGGGTTAAAAAGTTATTGAAAAATGAGAAGTTACTCTCTTTTGGTC2960.14793934486860388No Hit
GTTAATGGGAGAGCTATGAAGCAGAATTCACAAACTTTAAACCAAGAACATC2880.14394098419647944No Hit
AAGATGGGGTGGGGTTCGAACCTGTGCGGGATACGCTGTTGTCGGTGCCAGG2850.14244159894443278No Hit
ATAATGGGGGTTTTGTCCTTCTGGAAGAGGATGTTTGCTAGATATTTTAGAA2770.13844323827230837No Hit
CTTGACGGGGATCTAATCATGGGGAAAAATCCAAGATTGAGACAGAAATCAA2770.13844323827230837No Hit
ATTAGAGGGCAAGTACATGAAATATGAGAAGAGCTACCGCTTCCACTTGGGT2730.13644405793624614No Hit
ATGTGTGGGAGACAGTGACCCGGAAGTAGAAGTGGCCCTTGCAGGCAAGAGT2720.13594426285223057No Hit
ATTAAAGGGGGAGGAGAAAGATGGCGGCTACCTTACTAGCTGCTCGGGGAGC2700.1349446726841995No Hit
CAAACAGGGGACACAAGTTTATATAACAAACCTGCACATGTACCCCTGAACC2700.1349446726841995No Hit
GACTCAGGAAGACCAGAGCAGAGACAGGACACAAAGTCAAAAGAACTCAGTC2650.1324456972641217No Hit
TAATGGGCAGTGTTTACAAGTAATACCAGAAAGTTGCCAAACCCTTCTCTAT2640.13194590218010616No Hit
AATGTGGGTGTAAAACGTTTGGGAATCGTACACTTTCTAAGAAGGAAGATTT2630.13144610709609061No Hit
AAGCCTGGGCATCAAAGCAATCTTTGTGATATTACTTCGCTATTAAATAAAG2620.13094631201207504No Hit
GCATCTGGGAACTCTTGGGCAAAAAAACAAAATTAAGACAGTAATTAAAAAT2620.13094631201207504No Hit
CCATAAGGGAGTTCTTCTAACTAAGATGTTAAGCATTTGGCTTAAAGTGTAT2610.1304465169280595No Hit
AATGTAGGGGAAAAAAAGTAGTGTTTTTTTGGGGAACAGTATAATGTCTCAG2600.12994672184404393No Hit
GAAGAGGGGAGTCAGGTGGGTGCCAGGCCCTGGCCGTGGCGAAAGAGCCGGC2600.12994672184404393No Hit
TCTGCAGGGCCTCGATTTCCTGGGCTCAAGCAATCCTCCCACCTCAGCCCCC2540.12694795133995063No Hit
CTTGCTGGGCATCGAGTCTCCGCGCTGGAAGAACAGCAGTTCCTGATCATTC2520.12594836117191952No Hit
GGACAGGGGAGACCTAGCACTGCCGAGCGTTGGCAAGATGGACTTCAGCTTG2480.12394918083585729No Hit
GATCAAGGGCTTGGAATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGA2470.12344938575184175No Hit
TTCAGTGGGAAGCAAGTCCTGAGTGACCTACAAAGAGACTTAGACTCCCACA2470.12344938575184175No Hit
GCAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2470.12344938575184175No Hit
TAGCAGGGTGTACAAACTAGTTTTTATAGCTATCAACATTAGGAGTAACTTT2450.12244979558381064No Hit
AACCCTGGGAGTCCTAGTTTTAGACCTCATCTCCAAGATAGCATCCCATCTC2430.12145020541577953No Hit
ATCTATGGGCTGTGTCTGGGGTTCAGCTTCATCCCCAGTCACCAACTGCAGG2430.12145020541577953No Hit
TTCCAGGGGCATGCCTACAAACTGCGTGTGGCTAGATGGGCTTTCTTCGAAT2420.12095041033176397No Hit
AGCCAGGGCAGTATTTTTATATTTCTTACATTATCCTAATGATTGAAAACTC2420.12095041033176397No Hit
CATTGCGGGATGGAGTGGAAATTTGGAGTGACATCTGGGAGCAGCGAATTGG2420.12095041033176397No Hit
GTTACTGGGGAAGGAAAGTTTAATTACATTGCTCTTTTATTTTTTCCATTAA2410.12045061524774842No Hit
TCTCAGGGGTGAAAACTAAATTTTCTACTGTATTCCCAGCACGGGTGACTTC2390.11945102507971732No Hit
GCTCAAGGGGAAAAAAAGACTGATGTATGTATTCATAAGAACAGTTTAAGAT2370.11845143491168622No Hit
CATTATGGGGTAGAAGTTTTCCAGGTAAAGAGTCAGAAGGATGATATTTAGC2370.11845143491168622No Hit
ACACCAGGTGTGTGGGAAGCTGCAGTGAGAAAACCAAATAATCCTGGTTTGA2370.11845143491168622No Hit
CAGCTGGGGATTTGTTTGGGTTATAATATCATGTGGTTTATATATATTTACC2360.11795163982767065No Hit
AATTTAGGGGCCCGCTCGTCTGCCTGGCTGCGGGGTGACACGGGGCTTCGCC2360.11795163982767065No Hit
TTGCAAGGGGCGGCGCGAGGCGGGCGCTGCGGCGCCAGCAGCCCTGTGGTGG2360.11795163982767065No Hit
TAAGCAGGGAGCTTTTGGGAGCGTCCTTGAGCAAAGCGCCACGCCCCGCGAG2360.11795163982767065No Hit
CTTGTCGGGGGATTAGGAGTCATGACATTTCCCCATGAGAGTTCTAACAATT2350.1174518447436551No Hit
TAAGGCGGGAACAGAGCCAGACTCTGTCTCAATAAAGGAAAAAAAACAGTTC2350.1174518447436551No Hit
TCAAAAGGGTTCACATTAACAGGGAAAATTAGCTGAAAATAATGTCCAATTA2350.1174518447436551No Hit
CTAGTTGGGATTCCATTACACAAATGTGGCTCCTTAATAAACTGCTCTAATA2350.1174518447436551No Hit
TATTAGGGAAGATTCTCAGGTGATTTCTAAGAATCAGGGAAACAAAGAATAC2340.11695204965963955No Hit
TGTCTGGGCAATTGTGTTTACATTAATAGTGGTGTTTTTACCTATGAAATTA2320.11595245949160844No Hit
AACACTGGGGCCTTTCCGCTCGGCTGTTTTCCTGCGCAGGAGCCGCAGGGCC2310.11545266440759287No Hit
CTTACTGGGCATTAATAAATAATTACAGTATATTAAATTTCCATTCCATTCC2310.11545266440759287No Hit
GGTTTTGGGATTACTTTTCTTCCTACATTTTCCTTTTGCTGATTCATTTTTC2310.11545266440759287No Hit
TCAGAAGGGAGGTGCTGGCTGCTGTCTACAAGGCTCTGAGTGACCACCACAT2290.11445307423956178No Hit
ACTTAGGGGGATTGGAATAAAATAAAGAAAACAAAATGCATCTCACATGCCA2290.11445307423956178No Hit
GGCTACGAGTGAAAAGGCACCCTAATTTGGGATAGTGTGAAGAAACATAGTT2280.11395327915554622No Hit
CACCGTGGGTATGAAATGGGTACTTGGAGGATTTAAGTAACTTAGTCAAGGG2280.11395327915554622No Hit
GAAACTGGGCTCCAAAAAGAAGCTGCAACATGTGTGGACAATGGGCTTTTCA2270.11345348407153068No Hit
GTTCTTGGGGGCGTACCCGGGGCATCCTGGCGCCGGCGGCGGGTACTACCCA2270.11345348407153068No Hit
CTGTGCTCGGGCCCTAAATAAATCTAAAGTCTAAAGTTTTATTGATGTGAAA2270.11345348407153068No Hit
ATTTCAGGGAGAAGGCACAGGTGGCGAGATGAGCCGGGTACCAGCGTTCCTG2260.11295368898751512No Hit
TTAGGCGGGATCCTCCCCCAGAGCTGCCCCGTTCAGACCATGGATCCTGAGG2260.11295368898751512No Hit
ACTTGCGGGTCTCGCTCAGGGATGATTAGACAATGTGGAATTACATAACAGG2250.11245389390349957No Hit
TCTTAAGGGCTCAGTGAAAATTCTTAGCAATAAACTAAAATCAGGAATTCTG2240.111954098819484No Hit
AAATGAGGGGGGCGCAGGCGCAAGCCGGCAAGATGGCGGCGGCTGGGGCTGG2230.11145430373546847No Hit
TTCAGTGGGATGTGAAAACAATGTAAAGCTACACAATTAAAATAGTGTGGCA2220.1109545086514529No Hit
ATCGTTGGGCTCAGTGTAATGTCCATTCAAGGAGTATTAAATGAGGATTTCC2210.11045471356743734No Hit
AGTAACGGGTATCGTCTGATTTCCATTCTGGGTGATGCCAGGAAAAAGCAAT2210.11045471356743734No Hit
GGACAAGGGGCAGCTGAGTGGGGGTTACGGAGGCGGCTACGGTGGCCAGAGC2210.11045471356743734No Hit
ACAATTGGGAGTGATTTTTATAGCATGCACTATAAAGGCAAAGTGAAGAAGC2210.11045471356743734No Hit
TAAAATGGGCATGCAGGAGTTACAGACACTGGTTTCTTGGAAAATGGAGAGA2210.11045471356743734No Hit
AATGTCGGGGGTAGACACGTTTTAACTGAAATCAATCAAGATAACTTTATTC2200.1099549184834218No Hit
CTTTTCGGGACAAATTGTGTGGTTTCTTGAGGATTGGACTCTTGATGGATTT2180.10895532831539069No Hit
GATACTGGGAAGGCACATGTGTGATGTCCATTTGCCATAATGCGTCAGGACA2180.10895532831539069No Hit
GGCTCAGGGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGACATCTG2180.10895532831539069No Hit
TGTTGCGGGAAATCTAAGAGTTATTGACCTTAAAGAGGAGATAGAGAAAAAG2180.10895532831539069No Hit
TTACTGGGGATTTCCTGAAGTAAAGTCTTAAAATATATAACCAAATTAATTT2170.10845553323137515No Hit
ATTAGGGGGATCTGTGCCCGGGCTATGGAAGTAATATACAAATACCGGAAAT2170.10845553323137515No Hit
GCAGCCGGGGGGCGACGACCGAACGCCCCCGGGAACACCGGGCCCCGAGCTC2170.10845553323137515No Hit
AGCACAGGGAGGGGCAGCAGAACCAAGGCAAATATTTCAGCTGGGCTATACC2170.10845553323137515No Hit
CGCATAGGGAGAGTTAAAATTTTGATAAATGCATTGAAAACCTTTCAAGAAA2170.10845553323137515No Hit
AAATGGGGGGTCTAAGAGAATATACGCTTATGGAGCTGCTTGTGATGCACAT2160.10795573814735959No Hit
GAAATGGGCGGCATCATGATTTGTGTTTGCATTCTATATGTCCCTTCCTTCT2150.10745594306334402No Hit
TCAAACGGGTCTATGGGCGGGGGGGGATAATGGAAAAGAAGCAACGTAAGGC2150.10745594306334402No Hit
ATACACGGGAGAACTGAAGACAAGGTTAATTCAGAGAAAGGAATGCAGAATG2140.10695614797932847No Hit
GCCAATGGGACCGGCATTGCGGCCATGTCTGTCATGCGGCCGGAGCAGATCA2140.10695614797932847No Hit
TGCGCAGGGAAGAAAAACAAGACCAATAAGGTAATCGAATGATGTGAGATCA2140.10695614797932847No Hit
GCAGTGGGGTGTACTAACATGGTAGAGACCATCATATTTTTTAAGTAACGAT2140.10695614797932847No Hit
GGCCGCGGGGGTATTGAAAAGCTGTCAAGTTTATAGTGGCTGATACAAGTTT2130.10645635289531293No Hit
TCACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2130.10645635289531293No Hit
CAATATGGGATATTGTTCAGGGCTATATATCTGTACAAACTTTAAGCAATTG2130.10645635289531293No Hit
TTTGAGTGGGAAGGAAAATAATATTTACTTCCAAGAATGAAATTCAAGTTTT2130.10645635289531293No Hit
TTCAAGGGGAGCAAGACCCTGTCTCAAAACAAACAAACAAACAGAAATGTAT2130.10645635289531293No Hit
ACAGAAGGGAGATTTGGAGATGAAGTCATGGAATCTGCATTTAATAAATGTA2120.10595655781129737No Hit
GTTAAAGGGGAGCTAAGCCAGACCCGGGTGGCGGTGGCAGCTGCGAAACCCA2120.10595655781129737No Hit
AGCTTAGGGTATAATGAAGAAGTTTGTAGCAGAAACCTGAGAATATAGGTAT2120.10595655781129737No Hit
TGTTTAGGGCAGACTCCTAAAGCATTCCCACTCCACCACTCCAAAAGGTCAC2110.1054567627272818No Hit
TAGACCGGGTAAGCTTTCTGGGAAAGAGCAGAGTTTTACTTCTTTGGAAGTT2110.1054567627272818No Hit
TAATGCGGGTAGCTGTCCCTAATCACAGATCCTAAGGTAATATAATATAATT2110.1054567627272818No Hit
ACTTCTGGGAACTTTTGACTAGCATATGATTATTTGTGAGCTAGATCAACCT2100.10495696764326627No Hit
CTTCTAGGGTACTAATTGTGAAACACTGGAAATTAATGATGCAGACAACTTG2100.10495696764326627No Hit
ACGACAGGGGGGGGAGCAGAGGGAGATACAGAAACCGACAGGGGCCAGGCGC2100.10495696764326627No Hit
GCTGGTGGGGGACCTTTCCGAGTTGGCTGCAGATTTGTGGTGCGTTCTGAGC2090.1044571725592507No Hit
ACTAAGGGGGACAGTCCTAGATTTCTGTGAAATAAGAGTATTCTTCAACTTA2090.1044571725592507No Hit
GCAGGTGGGATACATGGTGACCTTTTCCACTTCCTTCTTATTTTTAGCATCC2090.1044571725592507No Hit
AGCATGGGGTCCGATCTTTAACTGGGATATCAATAATACCTCAACAAAGCTG2090.1044571725592507No Hit
TTGTTGGGGATATGATCTTTGGGGAGAAAAAGCCTTTAGTGAATTTTCTTGA2090.1044571725592507No Hit
AATTAGGGGCGATCTTCGTTGGGGTATTAATCAAATGACTTAAGGGATAGGG2080.10395737747523515No Hit
TAACATGGGGTAGATATGTGATTTTTTGAGACTGTATTTTTAGAGCTGAATT2080.10395737747523515No Hit
TTCAGCGGGCAGCTCCCATCCCTGGGTAATGGATGGTAGGATGATGGAGATT2080.10395737747523515No Hit
TATGATGGGGTATTTTGTGACCTTATGATTAGTTTTATCTTTCAACTGATTT2080.10395737747523515No Hit
GAGTTTGGGTCCAGGAGTATTGTCATTGAAAATGGAATCCTGCACCTAAAAG2070.1034575823912196No Hit
TTAGTTGGGGGCTGCAGTGAGCCATGATCATGCTAGTGCACTCCAGTGTGGG2070.1034575823912196No Hit
GCAGTGGGGGTAGATGAATCACTGTGATTATTGTTTTCAGTCGAAATACTTC2070.1034575823912196No Hit
TAATGTGGGTGGCAACGGGAGAGCAGTGTGCAGAATGTAATGCTCTTTTGTA2060.10295778730720405No Hit
TCAAGCAGGGGAATGCTCTGCTCCTGGAGAACAGCCCAAGTTCCTGGGCATC2060.10295778730720405No Hit
AGAGTAGGGGAAAGAAGCCAAGGGTCAAGAATGCAGAAGCAAAGGAAGGACT2060.10295778730720405No Hit
AAGTATGGGTATCTAGTTCTCAGAGCATTTGGAAAGGTTATCTTAAATGGCT2060.10295778730720405No Hit
GTTATGGGGAGCATGTGAGTAGTAGATAATCATGTCGATGACCTTTTTTTCT2050.1024579922231885No Hit
GAGCAAGGGCATAAAGTCTGGAGCAACACAGACCTTGCATTCCTTTGGGCAA2050.1024579922231885No Hit
TACCGGGTGAGGAAACTCATACAGTCTCTATTTGATTTGCAACACTGCCAAA2050.1024579922231885No Hit
CCTCTAGGGAAGAAAGGGTATCTGAACCAAGGGCCAGTGATATTCCTCTGTT2050.1024579922231885No Hit
ATTCGAGGGCATAGCAATCTCAACATATGTATGTTACATGCTTGTGTAACAG2050.1024579922231885No Hit
TTTATAGGGCAGCACTGCCCCTCAGCAGGGATGACTCAGCAGGGATGACTCA2040.10195819713917292No Hit
TCATGAGGGATTTGAAAAGGCTTCCATTAATATAATAGCTTTGCTTGCAGCC2040.10195819713917292No Hit
TAGCTTGGGATCACTGGGAAAACACAAAAAATTACACAACCCAGCAACAACA2030.1014584020551574No Hit
GGGCCGGGGAGGAAGAGTAGTGTGCATTCAGACATTCGGAATTTTTGCTAGT2030.1014584020551574No Hit
ACAACAGGGTCGGTGGGGGTGTGTTTGTATAAATCTTCAAGCGTAGTGTTAA2020.10095860697114183No Hit
AAGCGGGGGGCTCTGTCTACTATTGACTAACTTTCTGACCTTAGACAATCCA2010.10045881188712627No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)