FastQCFastQC Report
Sat 31 Dec 2022
SRR5845184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198518
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT10670.5374827471564292No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC9340.47048630350900167No Hit
CAAACAGGGGACACAAGTTTATATAACAAACCTGCACATGTACCCCTGAACC3180.160186985563022No Hit
CTTACTGGGCATTAATAAATAATTACAGTATATTAAATTTCCATTCCATTCC2830.1425563424979095No Hit
ACTTAGGGGGATTGGAATAAAATAAAGAAAACAAAATGCATCTCACATGCCA2640.13298542197684846No Hit
ATTAAAGGGGGAGGAGAAAGATGGCGGCTACCTTACTAGCTGCTCGGGGAGC2580.12996302602282916No Hit
TGTTTAGGGCAGACTCCTAAAGCATTCCCACTCCACCACTCCAAAAGGTCAC2570.12945929336382597No Hit
GACTCAGGAAGACCAGAGCAGAGACAGGACACAAAGTCAAAAGAACTCAGTC2570.12945929336382597No Hit
TTCAGTGGGAAGCAAGTCCTGAGTGACCTACAAAGAGACTTAGACTCCCACA2550.12845182804581953No Hit
TAAAATGGGCATGCAGGAGTTACAGACACTGGTTTCTTGGAAAATGGAGAGA2540.1279480953868163No Hit
GAAGAGGGGAGTCAGGTGGGTGCCAGGCCCTGGCCGTGGCGAAAGAGCCGGC2520.12694063006880987No Hit
CTTGACGGGGATCTAATCATGGGGAAAAATCCAAGATTGAGACAGAAATCAA2520.12694063006880987No Hit
GGCCGCGGGGGTATTGAAAAGCTGTCAAGTTTATAGTGGCTGATACAAGTTT2510.12643689740980665No Hit
GGGCCGGGGAGGAAGAGTAGTGTGCATTCAGACATTCGGAATTTTTGCTAGT2500.12593316475080346No Hit
CCTAGGGGGAAATCCGTCCTTGCAGAAACAGAAGGAATTTTACAGAAGCTAC2500.12593316475080346No Hit
GCACCCAGGGTTAAAAAGTTATTGAAAAATGAGAAGTTACTCTCTTTTGGTC2490.12542943209180024No Hit
GCATCTGGGAACTCTTGGGCAAAAAAACAAAATTAAGACAGTAATTAAAAAT2470.1244219667737938No Hit
ATAATGGGGGTTTTGTCCTTCTGGAAGAGGATGTTTGCTAGATATTTTAGAA2470.1244219667737938No Hit
TATGATGGGGTATTTTGTGACCTTATGATTAGTTTTATCTTTCAACTGATTT2450.12341450145578739No Hit
TGCGCAGGGAAGAAAAACAAGACCAATAAGGTAATCGAATGATGTGAGATCA2450.12341450145578739No Hit
ATTAGAGGGCAAGTACATGAAATATGAGAAGAGCTACCGCTTCCACTTGGGT2430.12240703613778095No Hit
TATTAGGGAAGATTCTCAGGTGATTTCTAAGAATCAGGGAAACAAAGAATAC2430.12240703613778095No Hit
TGTCTGGGCAATTGTGTTTACATTAATAGTGGTGTTTTTACCTATGAAATTA2420.12190330347877774No Hit
CCATAAGGGAGTTCTTCTAACTAAGATGTTAAGCATTTGGCTTAAAGTGTAT2420.12190330347877774No Hit
ATCGTTGGGCTCAGTGTAATGTCCATTCAAGGAGTATTAAATGAGGATTTCC2410.12139957081977452No Hit
GAACAGGGACTTCTGAAAAGAATTTTGTTGTTTCTCAGCTGTTTTCCAAAAG2380.11988837284276489No Hit
CTGTGCTCGGGCCCTAAATAAATCTAAAGTCTAAAGTTTTATTGATGTGAAA2360.11888090752475845No Hit
AAGCCTGGGCATCAAAGCAATCTTTGTGATATTACTTCGCTATTAAATAAAG2350.11837717486575525No Hit
GGACAGGGGAGACCTAGCACTGCCGAGCGTTGGCAAGATGGACTTCAGCTTG2340.11787344220675203No Hit
ACCGAAGGGAGGAGTACAAAGAAGTTATACCATGGAGAGTACTTTATGTGGC2340.11787344220675203No Hit
GTTCTTGGGGGCGTACCCGGGGCATCCTGGCGCCGGCGGCGGGTACTACCCA2330.11736970954774881No Hit
ATTAGGGGGATCTGTGCCCGGGCTATGGAAGTAATATACAAATACCGGAAAT2330.11736970954774881No Hit
GGCTACGAGTGAAAAGGCACCCTAATTTGGGATAGTGTGAAGAAACATAGTT2330.11736970954774881No Hit
AAGATGGGGTGGGGTTCGAACCTGTGCGGGATACGCTGTTGTCGGTGCCAGG2320.11686597688874562No Hit
GTTAATGGGAGAGCTATGAAGCAGAATTCACAAACTTTAAACCAAGAACATC2310.1163622442297424No Hit
TCTGCAGGGCCTCGATTTCCTGGGCTCAAGCAATCCTCCCACCTCAGCCCCC2310.1163622442297424No Hit
GCAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2300.11585851157073918No Hit
TAGCTTGGGATCACTGGGAAAACACAAAAAATTACACAACCCAGCAACAACA2300.11585851157073918No Hit
GGTTTTGGGATTACTTTTCTTCCTACATTTTCCTTTTGCTGATTCATTTTTC2290.11535477891173596No Hit
GCTCAAGGGGAAAAAAAGACTGATGTATGTATTCATAAGAACAGTTTAAGAT2290.11535477891173596No Hit
TGCTCCGGGTGATTAATCTGTATATAAGCCACATTGAAACATATTCAGGAAT2290.11535477891173596No Hit
CAGCTGGGGATTTGTTTGGGTTATAATATCATGTGGTTTATATATATTTACC2280.11485104625273276No Hit
GCCTCTGGGTAAGAGCCCTTACCATGTTGCTCATAATCTTATTTAATGCCAT2270.11434731359372954No Hit
CTTCTAGGGTACTAATTGTGAAACACTGGAAATTAATGATGCAGACAACTTG2270.11434731359372954No Hit
GATACTGGGAAGGCACATGTGTGATGTCCATTTGCCATAATGCGTCAGGACA2270.11434731359372954No Hit
GCAGGTGGGATACATGGTGACCTTTTCCACTTCCTTCTTATTTTTAGCATCC2270.11434731359372954No Hit
ATGTGTGGGAGACAGTGACCCGGAAGTAGAAGTGGCCCTTGCAGGCAAGAGT2260.11384358093472632No Hit
CTTGTCGGGGGATTAGGAGTCATGACATTTCCCCATGAGAGTTCTAACAATT2250.1133398482757231No Hit
CATTTAGGGTCATTAAAACTGAGAATAAGCTTCAACTCCAGCTGTCTGGCAC2250.1133398482757231No Hit
CTAGTTGGGATTCCATTACACAAATGTGGCTCCTTAATAAACTGCTCTAATA2240.1128361156167199No Hit
ATTTCAGGGAGAAGGCACAGGTGGCGAGATGAGCCGGGTACCAGCGTTCCTG2230.11233238295771668No Hit
AATGTGGGTGTAAAACGTTTGGGAATCGTACACTTTCTAAGAAGGAAGATTT2230.11233238295771668No Hit
ATCTATGGGCTGTGTCTGGGGTTCAGCTTCATCCCCAGTCACCAACTGCAGG2220.11182865029871346No Hit
ACAGCACGGGATCTGTTACTTTCTATTAAAGCATTATCTGTTTACCACATGT2210.11132491763971027No Hit
TAATGGGCAGTGTTTACAAGTAATACCAGAAAGTTGCCAAACCCTTCTCTAT2200.11082118498070705No Hit
ACACCAGGTGTGTGGGAAGCTGCAGTGAGAAAACCAAATAATCCTGGTTTGA2200.11082118498070705No Hit
CATTGCGGGATGGAGTGGAAATTTGGAGTGACATCTGGGAGCAGCGAATTGG2200.11082118498070705No Hit
ATATGCGGAGTGGACAAAGAACTTGTGGTCTTTTAAAACTGGGACTGATACT2190.11031745232170383No Hit
AATGTAGGGGAAAAAAAGTAGTGTTTTTTTGGGGAACAGTATAATGTCTCAG2190.11031745232170383No Hit
TACCGGGTGAGGAAACTCATACAGTCTCTATTTGATTTGCAACACTGCCAAA2190.11031745232170383No Hit
TAATGTGGGTGGCAACGGGAGAGCAGTGTGCAGAATGTAATGCTCTTTTGTA2180.1098137196627006No Hit
AAGTATGGGTATCTAGTTCTCAGAGCATTTGGAAAGGTTATCTTAAATGGCT2180.1098137196627006No Hit
AACCCTGGGAGTCCTAGTTTTAGACCTCATCTCCAAGATAGCATCCCATCTC2180.1098137196627006No Hit
TCTTAAGGGCTCAGTGAAAATTCTTAGCAATAAACTAAAATCAGGAATTCTG2180.1098137196627006No Hit
GGCACTGGGGAAGTATCTAAAGACAGTATATTTGTGAAGATCTTACAGAAAC2170.1093099870036974No Hit
TCTCAGGGGTGAAAACTAAATTTTCTACTGTATTCCCAGCACGGGTGACTTC2160.10880625434469418No Hit
GAGCAAGGGCATAAAGTCTGGAGCAACACAGACCTTGCATTCCTTTGGGCAA2160.10880625434469418No Hit
TCTCGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2150.10830252168569096No Hit
CGTTATGGGGGGAGTTCACAAACTTCATCATTCATTACCCCTTTTAATTATT2140.10779878902668777No Hit
AGCTGAGGGCTTAAAAAATAAAGTAACTACATTCATCTCTATATATGTTCAC2140.10779878902668777No Hit
TCTACTGGGATCCCTGCATAGTCTGATGCAGGGAATCAAGGAACCACACTTT2140.10779878902668777No Hit
AATTAGGGGCGATCTTCGTTGGGGTATTAATCAAATGACTTAAGGGATAGGG2130.10729505636768455No Hit
TGTTGCGGGAAATCTAAGAGTTATTGACCTTAAAGAGGAGATAGAGAAAAAG2130.10729505636768455No Hit
CAATATGGGATATTGTTCAGGGCTATATATCTGTACAAACTTTAAGCAATTG2120.10679132370868133No Hit
GTCATAGGGTACAGCCCTGCTGCTGGGGTTGATTTTGAACTGGAGTCATTTT2120.10679132370868133No Hit
TAATGCGGGTAGCTGTCCCTAATCACAGATCCTAAGGTAATATAATATAATT2100.1057838583906749No Hit
TCACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2090.10528012573167168No Hit
CACCGTGGGTATGAAATGGGTACTTGGAGGATTTAAGTAACTTAGTCAAGGG2090.10528012573167168No Hit
TAAGGCGGGAACAGAGCCAGACTCTGTCTCAATAAAGGAAAAAAAACAGTTC2090.10528012573167168No Hit
CGAAAAGGGGTGTGGTTGATTTGTACCCTAGGTGATCTTTGGCTTCCTCAAG2090.10528012573167168No Hit
CTAACTCGGGTGCAAAATCCAGTTCTTCCAAAAGCCACTTTATTTAGGGTTT2090.10528012573167168No Hit
CTTTGAGGGAAAGGTAACTGATGTTATTCGTCTTGTATGGCTTTTAGTATGT2090.10528012573167168No Hit
GGTATTGGGAGAATTCTCCACCCCGTATCACCAGAATAAACATTCTTCTCAG2090.10528012573167168No Hit
TAACATGGGGTAGATATGTGATTTTTTGAGACTGTATTTTTAGAGCTGAATT2080.10477639307266846No Hit
TAAGCAGGGAGCTTTTGGGAGCGTCCTTGAGCAAAGCGCCACGCCCCGCGAG2080.10477639307266846No Hit
TTTGAGTGGGAAGGAAAATAATATTTACTTCCAAGAATGAAATTCAAGTTTT2080.10477639307266846No Hit
GATCAAGGGCTTGGAATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGA2080.10477639307266846No Hit
TTCAAGGGGAGCAAGACCCTGTCTCAAAACAAACAAACAAACAGAAATGTAT2080.10477639307266846No Hit
GAGTTTGGGTCCAGGAGTATTGTCATTGAAAATGGAATCCTGCACCTAAAAG2070.10427266041366524No Hit
CTCGTTGGGATAATGGTAATTTTAAGCAGTATATCTATGAAATGTGTTGTTT2070.10427266041366524No Hit
AACACTGGGGCCTTTCCGCTCGGCTGTTTTCCTGCGCAGGAGCCGCAGGGCC2050.10326519509565883No Hit
ACAAAAGGGGCCCCTGCTTCCCAAATACAGTCAAAAAGCAAACAGGAGAGTC2050.10326519509565883No Hit
TCATGCGGGATAGAATAGTTGAAATGGGACAAGACATAGAAAACGTATGTAG2040.10276146243665561No Hit
TTGCAAGGGGCGGCGCGAGGCGGGCGCTGCGGCGCCAGCAGCCCTGTGGTGG2040.10276146243665561No Hit
ACTTGCGGGTCTCGCTCAGGGATGATTAGACAATGTGGAATTACATAACAGG2040.10276146243665561No Hit
TTGTTGGGGATATGATCTTTGGGGAGAAAAAGCCTTTAGTGAATTTTCTTGA2040.10276146243665561No Hit
TAGCAGGGTGTACAAACTAGTTTTTATAGCTATCAACATTAGGAGTAACTTT2040.10276146243665561No Hit
ACAGAAGGGAGATTTGGAGATGAAGTCATGGAATCTGCATTTAATAAATGTA2030.1022577297776524No Hit
GAGCCGGGGAGGTTGGTGGGGTCGGTTTGAGAAGATATAGAAATTCTATTTT2030.1022577297776524No Hit
AGCCAGGGCAGTATTTTTATATTTCTTACATTATCCTAATGATTGAAAACTC2030.1022577297776524No Hit
TAGACCGGGTAAGCTTTCTGGGAAAGAGCAGAGTTTTACTTCTTTGGAAGTT2020.10175399711864919No Hit
AATGTCGGGGGTAGACACGTTTTAACTGAAATCAATCAAGATAACTTTATTC2020.10175399711864919No Hit
TCATGAGGGATTTGAAAAGGCTTCCATTAATATAATAGCTTTGCTTGCAGCC2020.10175399711864919No Hit
AAATGGGGGGTCTAAGAGAATATACGCTTATGGAGCTGCTTGTGATGCACAT2020.10175399711864919No Hit
ATACATGGGCATGAGGATTTATTTGCTGACTGCACGTAAGAGATAAAAGAGA2010.10125026445964597No Hit
TTCAGTGGGATGTGAAAACAATGTAAAGCTACACAATTAAAATAGTGTGGCA2010.10125026445964597No Hit
TCAAACGGGTCTATGGGCGGGGGGGGATAATGGAAAAGAAGCAACGTAAGGC2010.10125026445964597No Hit
CGCATAGGGAGAGTTAAAATTTTGATAAATGCATTGAAAACCTTTCAAGAAA2010.10125026445964597No Hit
GGACAAGGGGCAGCTGAGTGGGGGTTACGGAGGCGGCTACGGTGGCCAGAGC2000.10074653180064275No Hit
GTAAACGGGATGCGCGTTGCGCGCCGGACGCGGAACGTCTGCCGGTGTCCCC2000.10074653180064275No Hit
ATTCGAGGGCATAGCAATCTCAACATATGTATGTTACATGCTTGTGTAACAG2000.10074653180064275No Hit
ACTTCTGGGAACTTTTGACTAGCATATGATTATTTGTGAGCTAGATCAACCT1990.10024279914163955No Hit
AATTAAGGGCTGCTGGTGGGAAGGAAATCCAGACATTCTTGGAACTGAAGGT1990.10024279914163955No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)