FastQCFastQC Report
Sat 31 Dec 2022
SRR5845180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151658
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC7640.5037650503105672No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT7150.4714555117435282No Hit
TTAGCCAGGGGTAAGAAACTGGTAATCATTTTTTCAAAGTACTTGTGCCACT4000.26375133524113464No Hit
ATCGTTGGGAGGCCCAGGGTGGCTGCTGCTTCCTGCTAAGTGGGGGAGGTGA2590.1707789895686347No Hit
AGCAACGGGCACGATCAACACCAACCTGGGTGACCTGCAGTTCCTGGCCATC2360.15561328779226943No Hit
GATCATGGGTACTCCAGCCATGCAGGGCGCCTCGAGCAGCGTGCTGGCTGAG2350.15495390945416662No Hit
CTCTTCGGGGAAGAATCTTTAAGATAAATTGAAAGTGCAATTTCTGGTTACG2250.14836012607313825No Hit
TTTGCAGGGGACTGAGAAATTGGAACATCGATCAGTTAAAAAGGCTGGCATT2130.1404475860159042No Hit
TTCTGTGGGTGTGGCCAGAAACTCCTGCCTTGGGATTTGCCGAATACTAACT2080.13715069432539004No Hit
TGTCACAGAAGAGGGGTAAACCATACACACAAATGAGAAAGAGGAAGGGCTC2070.13649131598728717No Hit
TTAGCCGGGATCTAGAGGAGGGATCCTCATCGACATGGTCCTGAACCGCATG2060.13583193764918436No Hit
TACAGTGGGAGCGCAGAAGCCTCTGCTGTCCTTGGAGGAGACTCTGAATGTG2060.13583193764918436No Hit
GTATAAGGGTTGTGTAGTAACACAAAGCTGCAGGTTTTAATTTTGCTTTGTC2040.13451318097297868No Hit
CACCAAGGGATTACAACTTGCTCTTGAGATAACTACCAACTCTGATGATAGG2020.133194424296773No Hit
TTTGCTGGGATCTTACTCTAGGTTTGTAAAGTTCCCTATTATTATTTCTTTG2010.13253504595867016No Hit
ATCTTGGGGTAATTTAAATTTTAAAAACCTCCAAAAAACCAATTTCCAAGTA2000.13187566762056732No Hit
TTCACTGGGCAAATGAATTCCTTCGAAATGTATTTGAACTTGGACCCCCAGT1960.129238154268156No Hit
AGTGCCGGGAGAATTGTTCCAAAAGAAAATAGGTCAGCTTTTAAAACAAAGC1950.12857877593005315No Hit
GCTTGGGAAGACATGTGTGGACGAGATGCAGAAGATTACGCTATTTCTTGCC1930.12726001925384747No Hit
TTAAAAGGGGTTTAAACAAAATAGATACAAGCTCTGGCTTCTAGACACCTGC1920.12660064091574463No Hit
CACTGCGGGTTACTGAAGATGCCAGAGTTTTGAAAACTGAATGTGATTCTTG1920.12660064091574463No Hit
GAGGCTGGGCCAGAGTCAAGCCCCAGCAACTACAGTGCTTTACAGGAAGTGT1910.1259412625776418No Hit
GAGTATGGGTGAAATTAACTCAGTAGAAGGGCTTAAGAACAGATTTGGCCTA1890.12462250590143611No Hit
ACGCTAGGGACAGCCACCCCCAGAGCAAGAGAATGTGCAGTATGCTGGCCTT1860.12264437088712761No Hit
ATTTTAGGGCTATTTTTTCTGGGAGAGGGGGGGATTGATTAGAAAAAAATGT1860.12264437088712761No Hit
CTTGCCGGGAACAGATAAATACATTACACCTTTCAAAGAATAAATGAAAAAT1860.12264437088712761No Hit
GAACTGGGGCATTGTTCACTTTATTATTGAACTTTCTTTATACTTTCATGTG1850.12198499254902478No Hit
TATTTTGGGCAGGCAGATGAGTCCCTAGCTTAAACAAGAAAATGAACTATGC1830.1206662358728191No Hit
ACCGTTGGGTGACATTAACATATGAAAAAACAGCTACTTGTGCCTGACTATG1810.11934747919661343No Hit
GGTACAGGGAGTTTGTTTAGTTTTTTCTTACTTTTAAATTCCAACTTAAAAT1810.11934747919661343No Hit
GCGGTTGGGAACAGAAAATTTCCTGCAAGCTCCCCTAAGCTTGAAGAGACAA1810.11934747919661343No Hit
GCATTTGATTTTTTATTATTTTATGTTCCCCCGTTAGATTGTTTTAAGTGTT1810.11934747919661343No Hit
CAAACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1800.1186881008585106No Hit
AAAGATGGGGGGATATAATGATTATATCCTACACTTCTGCATTACTCCACAG1800.1186881008585106No Hit
TGTAACGGGTGAGTGCCCAGTGGATGGGTGCTATGGCAGCAGAGATTTTACA1790.11802872252040775No Hit
CTCTTGGGAGACATTGAAAATAAGGGACAACACCAGAAAAAACTGGTTAGCG1790.11802872252040775No Hit
TGATAAGGGGGAAAAAGAGTTGAGCAGGATTCTCTTAGGCTATGAGAGAGTC1790.11802872252040775No Hit
TTAAGCGGGAAGAAAGACTTTAGTAGTAAGGAGAATTCCACCAGGGTACAAA1780.11736934418230492No Hit
GATTAAGGGATTCAGCTCCAGTGTGTGCCCCATACTGAAACTTTCCTTATTG1770.1167099658442021No Hit
AGACTAGGGAATTATCTTTTAGCCTTCCTCCTAATTTTCTAATTGAAACTTC1770.1167099658442021No Hit
TGTGCAGGGTGAAAATGCAATTCAGACATGATGATCCAGTTCCCCATCACAC1760.11605058750609924No Hit
AGTCTGGGGGTATCCTGTCAGACACGTCGGACGAGGAGGACGCGAGTGTCAC1760.11605058750609924No Hit
GGTTCCGGGTGAAAGGAGATGACCTTCAGGCCATTAAGAAGGAGCTGACCCA1760.11605058750609924No Hit
GCTGTGGGGGAGTTGGGGTTACAGCTGTTCAGAACAGGCTCAGGGCACATCC1760.11605058750609924No Hit
CAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1760.11605058750609924No Hit
CTAAATGGGTTATTAATCCCACTACTAGATATCTACCCAGAGGAAAAGAAAT1750.11539120916799642No Hit
GCACGGGTTCCCCAGGCATATTTCAAAATGGTTACTTTTCTCCTACCCCTGC1750.11539120916799642No Hit
GGCATGGGGAGTCTCAATTGGGCATATCTAAGTTACTGGCCTTAAAGAGGAG1750.11539120916799642No Hit
GCGACCCCAGCAGTGCCAGTCAGTTCTGAGCGAGCGGCCCCTGGACAGTTGC1740.11473183082989356No Hit
TTGCAAGGGATCCAAGATCAATTCCCTCTCTCAGCACAGCCTGGGGAGGGGG1730.11407245249179074No Hit
AACTCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1730.11407245249179074No Hit
AAGCACGGGGATCTTTGAGCCTAGGATGCTAAGGTTGCAGTGAGCCAAGATG1730.11407245249179074No Hit
GCATTAGGGACGGAACCAAAAACAAACTCTCCAAGTATATTCACACATTCAA1720.11341307415368791No Hit
CTAACAGGGACAGATATGCAAAATTCCAGTACGAATGTGGGAATTACTCAGG1700.11209431747748223No Hit
GACAATGGGCAATCTGGGTACTGGTGAAGGTTGTTTGATAATAAAGACACAG1690.11143493913937938No Hit
AAAGCAGGGTAAATATGGAAAAATATTAAATCGATTGAAAGCATGTTTTCAG1690.11143493913937938No Hit
AAACATGGGGGGTCTGTTGCTCTTATTGGCTTCCAAATGTGCATGGCAAAGA1690.11143493913937938No Hit
TCTTCGGGGCCCTATTTTCAGCTACTATTTGAATATTCTTGAACACCACCAC1690.11143493913937938No Hit
GAAATAGGGCTCCTGAGCTCAGGCAGTCTGCCTGCCTTGACCTCCCAAAGTG1690.11143493913937938No Hit
TTAGAAGGGTGGATATAAATGCCAGCACCTAGACCAAAGTTTTGTTTTGTTT1680.11077556080127655No Hit
ACCAACGGGAATCCCAGCTACTTTGGTGGCTGAGGCATGAGAATCACTTGAA1680.11077556080127655No Hit
AGATTAGGGCAGCCTGGGCACAATCTAGCCTGGGCAACAAAGGCGAAACTCC1680.11077556080127655No Hit
TTAACTGGGCAGGATCAGAGGATGTACCCCATTCCCGCTACCCTTAGGTCTA1680.11077556080127655No Hit
AGTCGGGGGCCCTTGTTTAGGGAGAACAAAACAACACATCCAAAAAAATGAC1680.11077556080127655No Hit
AGGCTCGGGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAA1670.11011618246317373No Hit
TCGCAGGGGGGCTGACTGGCGGCAGGCTCGCCGCGGCGCGGAGTCCCGGCTG1660.10945680412507088No Hit
TGAATAGGGATTGTCGCCAAAGTTTGAGAAGCACTCAGGCTTTATGCTCCTT1660.10945680412507088No Hit
ACACATCCCCAAGGAGATCGGCCACGAGAAGCACCGGGACTGGGTCAGCTCT1660.10945680412507088No Hit
GTCACGGGGGGGATTGCAGGTCATCATCAGATTTGAAATATTTAAAGTGGAT1660.10945680412507088No Hit
AAAAGCGGGAAAGACGACTATATGACACTTGAATATTGATATTCAACCTTAA1660.10945680412507088No Hit
CTTCAGGGTGTTTGCTGTATGCCAAATGCCCTTTAATTCTCTGGCATGCTTA1650.10879742578696805No Hit
CACGTTGGGGAGTTCATCTGCGCTCTGTCCATCACCTCCAGGGGCAGCTTTG1650.10879742578696805No Hit
GACCTGGGGTGATGGGATCACATGGCAGCATGTACCTGATGCAAGGTACTGC1650.10879742578696805No Hit
CATGTCGGGTGCCCTTTGTTGAAATGTCTTTTGCCCATTTTCCAATTGGATT1650.10879742578696805No Hit
GCAATAGGGGGAAATAGAACCCTTGGCTTTTGTGTGGCAAGAGCAATGGAAA1640.10813804744886522No Hit
AACTAAGGGAGGCTGCATTTACTCTTGCTAAATAAAAGTGGGAGTGGGGCGT1640.10813804744886522No Hit
TTTGGGGGGGAGGGTCATCTTTTCAGAGAAATTTAAGGATTAAGACAGCATC1640.10813804744886522No Hit
TAAACAGGGATATATATCACGTTACCAAAAAAGAAGGTGCAGAAAAATTGAT1630.10747866911076237No Hit
CATCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1630.10747866911076237No Hit
GCTACAGGGACCCATTTTTGTGGGTGAGGAACTGGAAAGGGATGGATTTCCT1630.10747866911076237No Hit
AGCACAGGGGGGCACCAGCCCTCTTGGTAGCTGGTGTGGAGGGCCGGTGTCC1630.10747866911076237No Hit
TAAAGGGGTGTTACTTAGGCTGTTAGATCCAAGTTGATTTTTGTGTCTACAG1620.10681929077265954No Hit
AAGTAAGGGAAGACCAGCCTGTCCAACATGGTGAAACCCTGTCTCTACTAAA1620.10681929077265954No Hit
ATGTGGGGTTCAGCATGACCAAACACTGGGGGGTTGGGATAGGTGAGTTCTA1620.10681929077265954No Hit
GCCTCCTCATCCTTATTATCAGCACTCAGGCGCCCACTGTCTGCTCGAACTG1620.10681929077265954No Hit
TCCGCGGGGATGTGATAAAAGCAGGATTCAGCTGGACAGGCACAGATATTAT1620.10681929077265954No Hit
ATGTTGGGGGAAATGATTACTCTGTAAAGCAATGACATAATAAAATACAAAT1610.10615991243455669No Hit
TTGAACGGGACATCATCATCTACGCCTGGTCCATCGGCGGCTTCACTGCCAC1610.10615991243455669No Hit
TGCACCGGGGATGGGCCCTGTCCTTCATGTAAGCCTGGGTGGGGTATGCTTC1610.10615991243455669No Hit
CTAATAGGGAGACAATCTAAATATATCATCAGTTTTTAAAAGTGCCTATGTG1600.10550053409645387No Hit
GCCTCTGTGGCGGCGATCTATCACAGGGCTGGCAGTGTGCAGGAGTGGGGTT1600.10550053409645387No Hit
CGGATTGGGGTAACTGGTGGCTATCCATTTGAAGCTTACAAAAATTGTTTTC1600.10550053409645387No Hit
ATCTAGGGGACTTGCGCACGCTGATCAACGAGCTAGTGAAAGGGATCTTGCC1600.10550053409645387No Hit
AAAACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1600.10550053409645387No Hit
ATGCCTGGGGGACTATTCCGCTTACCGTTCTTCCCTTCTCTTTTGGGCCCTC1600.10550053409645387No Hit
TGCATCGGGTATTTGTGTCATGTCTGCTTTCTTTCAAGAGTATTTTGTAGTT1600.10550053409645387No Hit
CACTTTGGGGGGGCTTCCTTCAGTGAAGCCCAACACACAAAACGTCCCTTTC1590.10484115575835104No Hit
GCGCGCGGGAAGACACTGGCATAAAAAGTAAAAGTTTTATCTTAAGCTATTT1590.10484115575835104No Hit
AATCAAGGGAGTATGCAGAGACTTTCCTCATTAAAAAAATTATAAGTTTTTA1590.10484115575835104No Hit
AACATGGCCTGCTCTAAGATAAGGCTTTCTGTTTACTAACCTGGAGCTGCTC1590.10484115575835104No Hit
AGCCTGGGCAACGGAGTGAAACTCGTCTCAAAAACCAAAACCAAAACAAAAC1590.10484115575835104No Hit
CAACGGGGCCGATCTCTATTAGGGGACAGGCCCTGGGAGGCTGCTGTGCTCT1590.10484115575835104No Hit
ACAAACGGGCTATAAGTTCTAGAAATCTAATAAATAATGCCAAATTTTTAGC1590.10484115575835104No Hit
ATACTAGGGTCTAGGAATGGGGCAAAAGAATGTATGGCTGGAACTTCAGGGT1580.10418177742024819No Hit
ATCTACGGGACAGCCAGGGTATCCAGGATGCCAACCGTTGGGTCCCTACCCT1570.10352239908214536No Hit
ACACTAGGGTTCACGCAGTGGCCAATAATCAAGACAAACTGGGATTTGAGGA1570.10352239908214536No Hit
CATATAGGGTTGGGAGGCGGAGGTGGGAGGATAGCTTGAGGCCAGGAATTCC1570.10352239908214536No Hit
AATCCAGGGAGATAAGGCTAACCTATGCTCTTATGATCTGGGTGAGATTGCA1560.10286302074404251No Hit
TTACACGGGTATTCATTAAAGAATGCTACTGGCCTTGGTTTAATTGTGACTT1560.10286302074404251No Hit
TGCATCGGGGTTTCATACCAACTGAGGTGTCTGTTCTGGTCCGTGCTATTTC1560.10286302074404251No Hit
GCACAAGGGATAAAATATTGAGTGTGAGATGATATGTAGTCACTTTACTTTT1550.10220364240593968No Hit
CTAGAGGGGACCAGAGAGTGGTGGGAAAGGCCAGTGGCCAGGAGGAGCATTC1540.10154426406783686No Hit
CCCACAGGGATCGAAGAGATAGCTGCACTTCTATTACTATTGCAGCACTATT1540.10154426406783686No Hit
AAATGCGGCTACATAAGTTTTCATTTATTTGTGTTTTATTTATTACAGGGCT1540.10154426406783686No Hit
ACCAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1540.10154426406783686No Hit
ATACGTGGGATGAGAAAATGGAATGTTTCAAAGTACAGCCAGTCATTGGGGA1540.10154426406783686No Hit
GTTGTTGGGGCTCACCTAATGAAAAATAATGACATAATTTTCAGAACGCTTT1540.10154426406783686No Hit
ATAGCTGGGGACATAAGTGTTAACCAATCACTCTCTCTCTTTACAGATACTA1540.10154426406783686No Hit
GATGGTGGGATCACCGGCCCCATCGCCAAACTCATCCATGAGCAGGAGCAGC1540.10154426406783686No Hit
TTAGTCGGGTATATAAGCCTGGTTAAATTGGTCATCTAAAGCTGTCAAATAA1530.100884885729734No Hit
CACTAGGGATGCACCCAACACTGGAGCACCAAATATATGAAGCAAATATTAT1530.100884885729734No Hit
TCGTTGGGGCCGATCTCGAAACGGGTACAGAACAACGGTTTTTCTTCTGCCA1530.100884885729734No Hit
GCTCACGGGTCTAGAGAGTAAGGGTAACAGATTTACATAGAATAACAGCCTC1530.100884885729734No Hit
TCAGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1520.10022550739163118No Hit
TCGGATGGGGGTTCACATTAGATAGTCAAGAAATGTAAATAAGAAGCTGAAC1520.10022550739163118No Hit
AAATCTGGGTTATAGGTGCTTAGTAAATTTTTGTTGAATGAAAGATGACAAA1520.10022550739163118No Hit
CAATTAGGGATTGACAAGCTTTCCAGAAGCAATTTTATAACAGATTTCATTT1520.10022550739163118No Hit
TCAGCTGGGAAGACACAGCAACTGTTCGATGATATTGAAGTATTCAAAATAT1520.10022550739163118No Hit
TCGACAGGGCTCTCGCCAGGCGTCCTCGTGGAAGTGACATCGTCTTTAAACC1520.10022550739163118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)