FastQCFastQC Report
Sat 31 Dec 2022
SRR5845179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150378
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT7560.502733112556358No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC7120.47347351341286625No Hit
TTAGCCAGGGGTAAGAAACTGGTAATCATTTTTTCAAAGTACTTGTGCCACT3400.22609690247243613No Hit
ATCGTTGGGAGGCCCAGGGTGGCTGCTGCTTCCTGCTAAGTGGGGGAGGTGA3120.20747715756294138No Hit
ATCTTGGGGTAATTTAAATTTTAAAAACCTCCAAAAAACCAATTTCCAAGTA2620.1742276130817008No Hit
AGCAACGGGCACGATCAACACCAACCTGGGTGACCTGCAGTTCCTGGCCATC2450.16292276795807897No Hit
TTCACTGGGCAAATGAATTCCTTCGAAATGTATTTGAACTTGGACCCCCAGT2210.14696298660708348No Hit
TTAGCCGGGATCTAGAGGAGGGATCCTCATCGACATGGTCCTGAACCGCATG2130.141643059490085No Hit
GTATAAGGGTTGTGTAGTAACACAAAGCTGCAGGTTTTAATTTTGCTTTGTC2070.13765311415233614No Hit
TACAGTGGGAGCGCAGAAGCCTCTGCTGTCCTTGGAGGAGACTCTGAATGTG2060.1369881232627113No Hit
CTCTTCGGGGAAGAATCTTTAAGATAAATTGAAAGTGCAATTTCTGGTTACG2060.1369881232627113No Hit
ACACTAGGGTTCACGCAGTGGCCAATAATCAAGACAAACTGGGATTTGAGGA2040.13565814148346167No Hit
ACGCTAGGGACAGCCACCCCCAGAGCAAGAGAATGTGCAGTATGCTGGCCTT2030.13499315059383687No Hit
TTTGCAGGGGACTGAGAAATTGGAACATCGATCAGTTAAAAAGGCTGGCATT2010.13366316881458723No Hit
GATTAAGGGATTCAGCTCCAGTGTGTGCCCCATACTGAAACTTTCCTTATTG1990.1323331870353376No Hit
AAATCTGGGTTATAGGTGCTTAGTAAATTTTTGTTGAATGAAAGATGACAAA1980.1316681961457128No Hit
GAGGCTGGGCCAGAGTCAAGCCCCAGCAACTACAGTGCTTTACAGGAAGTGT1980.1316681961457128No Hit
CACTGCGGGTTACTGAAGATGCCAGAGTTTTGAAAACTGAATGTGATTCTTG1970.131003205256088No Hit
CACCAAGGGATTACAACTTGCTCTTGAGATAACTACCAACTCTGATGATAGG1960.13033821436646317No Hit
TTCTGTGGGTGTGGCCAGAAACTCCTGCCTTGGGATTTGCCGAATACTAACT1960.13033821436646317No Hit
AGATTAGGGCAGCCTGGGCACAATCTAGCCTGGGCAACAAAGGCGAAACTCC1950.12967322347683835No Hit
GATCATGGGTACTCCAGCCATGCAGGGCGCCTCGAGCAGCGTGCTGGCTGAG1940.12900823258721356No Hit
CTAAATGGGTTATTAATCCCACTACTAGATATCTACCCAGAGGAAAAGAAAT1940.12900823258721356No Hit
GCGGTTGGGAACAGAAAATTTCCTGCAAGCTCCCCTAAGCTTGAAGAGACAA1930.12834324169758873No Hit
ACAAACGGGCTATAAGTTCTAGAAATCTAATAAATAATGCCAAATTTTTAGC1930.12834324169758873No Hit
ATACTAGGGTCTAGGAATGGGGCAAAAGAATGTATGGCTGGAACTTCAGGGT1900.1263482690287143No Hit
TTAAAAGGGGTTTAAACAAAATAGATACAAGCTCTGGCTTCTAGACACCTGC1890.1256832781390895No Hit
TTGAACGGGACATCATCATCTACGCCTGGTCCATCGGCGGCTTCACTGCCAC1890.1256832781390895No Hit
GGTACAGGGAGTTTGTTTAGTTTTTTCTTACTTTTAAATTCCAACTTAAAAT1890.1256832781390895No Hit
TAAAGGGGTGTTACTTAGGCTGTTAGATCCAAGTTGATTTTTGTGTCTACAG1880.1250182872494647No Hit
AACTCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1870.12435329635983987No Hit
CGGATTGGGGTAACTGGTGGCTATCCATTTGAAGCTTACAAAAATTGTTTTC1870.12435329635983987No Hit
GCCTCTGTGGCGGCGATCTATCACAGGGCTGGCAGTGTGCAGGAGTGGGGTT1850.12302331458059024No Hit
GCGACCCCAGCAGTGCCAGTCAGTTCTGAGCGAGCGGCCCCTGGACAGTTGC1850.12302331458059024No Hit
CAAACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1840.12235832369096543No Hit
CAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1840.12235832369096543No Hit
AAACATGGGGGGTCTGTTGCTCTTATTGGCTTCCAAATGTGCATGGCAAAGA1840.12235832369096543No Hit
TCCGCGGGGATGTGATAAAAGCAGGATTCAGCTGGACAGGCACAGATATTAT1840.12235832369096543No Hit
TGTCACAGAAGAGGGGTAAACCATACACACAAATGAGAAAGAGGAAGGGCTC1830.12169333280134062No Hit
GCTGTGGGGGAGTTGGGGTTACAGCTGTTCAGAACAGGCTCAGGGCACATCC1830.12169333280134062No Hit
GCTTGGGAAGACATGTGTGGACGAGATGCAGAAGATTACGCTATTTCTTGCC1830.12169333280134062No Hit
TTGCAAGGGATCCAAGATCAATTCCCTCTCTCAGCACAGCCTGGGGAGGGGG1820.1210283419117158No Hit
CTAACAGGGACAGATATGCAAAATTCCAGTACGAATGTGGGAATTACTCAGG1820.1210283419117158No Hit
GAAATAGGGCTCCTGAGCTCAGGCAGTCTGCCTGCCTTGACCTCCCAAAGTG1820.1210283419117158No Hit
CTTGCCGGGAACAGATAAATACATTACACCTTTCAAAGAATAAATGAAAAAT1820.1210283419117158No Hit
GACCTGGGGTGATGGGATCACATGGCAGCATGTACCTGATGCAAGGTACTGC1810.120363351022091No Hit
GCACGGGTTCCCCAGGCATATTTCAAAATGGTTACTTTTCTCCTACCCCTGC1800.1196983601324662No Hit
ATCGCTGGGTACATCAGACAAACTTTAAAGCAACAGCAGTGAAAAATAACAA1790.11903336924284137No Hit
GCCTCCTCATCCTTATTATCAGCACTCAGGCGCCCACTGTCTGCTCGAACTG1790.11903336924284137No Hit
GAGTATGGGTGAAATTAACTCAGTAGAAGGGCTTAAGAACAGATTTGGCCTA1790.11903336924284137No Hit
AAAGATGGGGGGATATAATGATTATATCCTACACTTCTGCATTACTCCACAG1780.11836837835321656No Hit
GACAATGGGCAATCTGGGTACTGGTGAAGGTTGTTTGATAATAAAGACACAG1770.11770338746359175No Hit
AAAGCAGGGTAAATATGGAAAAATATTAAATCGATTGAAAGCATGTTTTCAG1760.11703839657396693No Hit
GGTGTCCTGCTGCCCTTCTTTGACCCTGACACCAACATCGTCTACCTCTGTG1760.11703839657396693No Hit
TTAAGCGGGGTGCAACATACAGGTAGGTCTTCAGCATAAGCTGAAATATATG1750.11637340568434212No Hit
ATAGCTGGGGACATAAGTGTTAACCAATCACTCTCTCTCTTTACAGATACTA1740.1157084147947173No Hit
AAGTAAGGGAAGACCAGCCTGTCCAACATGGTGAAACCCTGTCTCTACTAAA1730.11504342390509249No Hit
TGATTCGGGAGTTCAAGAAAATGAGGAGAAAAGGCTACAGCTTCAGAATGCG1730.11504342390509249No Hit
GTTATAGGGTCCTGTCCTTTGCATCAACATAGATATAGCTAGAGGCCATTAT1730.11504342390509249No Hit
GGCATGGGGAGTCTCAATTGGGCATATCTAAGTTACTGGCCTTAAAGAGGAG1730.11504342390509249No Hit
TGTGCAGGGTGAAAATGCAATTCAGACATGATGATCCAGTTCCCCATCACAC1720.1143784330154677No Hit
ATGTTGGGGGAAATGATTACTCTGTAAAGCAATGACATAATAAAATACAAAT1720.1143784330154677No Hit
GAACTGGGGCATTGTTCACTTTATTATTGAACTTTCTTTATACTTTCATGTG1720.1143784330154677No Hit
TATTTTGGGCAGGCAGATGAGTCCCTAGCTTAAACAAGAAAATGAACTATGC1720.1143784330154677No Hit
TGAATAGGGATTGTCGCCAAAGTTTGAGAAGCACTCAGGCTTTATGCTCCTT1710.11371344212584286No Hit
TGCACCGGGGATGGGCCCTGTCCTTCATGTAAGCCTGGGTGGGGTATGCTTC1710.11371344212584286No Hit
TTTGCTGGGATCTTACTCTAGGTTTGTAAAGTTCCCTATTATTATTTCTTTG1700.11304845123621807No Hit
TGTTGCGGGATTTGGGAATTATTAAAAAGGCAAACAATGAATGTTATTAGGA1700.11304845123621807No Hit
AACTCCGGGTGCATGCGGGATGTGGTGAGTGGATCTCTTGATACATCAATCC1700.11304845123621807No Hit
TAAACAGGGATATATATCACGTTACCAAAAAAGAAGGTGCAGAAAAATTGAT1690.11238346034659326No Hit
TCCGAGGGTGGTGGTTGGCTCCACCATCAATGATGTTTTAGCAGTAAACACA1690.11238346034659326No Hit
AGGCTCGGGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAA1690.11238346034659326No Hit
CATGTCGGGTGCCCTTTGTTGAAATGTCTTTTGCCCATTTTCCAATTGGATT1690.11238346034659326No Hit
GCTACAGGGACCCATTTTTGTGGGTGAGGAACTGGAAAGGGATGGATTTCCT1690.11238346034659326No Hit
GCAGTAGGGCCTAAAAAATAGGAAACTATCGTCTCCATTTTACATATGACAA1680.11171846945696844No Hit
AGTCTGGGGGTATCCTGTCAGACACGTCGGACGAGGAGGACGCGAGTGTCAC1680.11171846945696844No Hit
TGATAAGGGGGAAAAAGAGTTGAGCAGGATTCTCTTAGGCTATGAGAGAGTC1680.11171846945696844No Hit
AGCACAGGGGGGCACCAGCCCTCTTGGTAGCTGGTGTGGAGGGCCGGTGTCC1680.11171846945696844No Hit
CATATAGGGTTGGGAGGCGGAGGTGGGAGGATAGCTTGAGGCCAGGAATTCC1670.11105347856734363No Hit
TTAAGCGGGAAGAAAGACTTTAGTAGTAAGGAGAATTCCACCAGGGTACAAA1660.11038848767771882No Hit
GCATTAGGGACGGAACCAAAAACAAACTCTCCAAGTATATTCACACATTCAA1660.11038848767771882No Hit
TCGCAGGGGGGCTGACTGGCGGCAGGCTCGCCGCGGCGCGGAGTCCCGGCTG1650.109723496788094No Hit
ATGTGGGGTTCAGCATGACCAAACACTGGGGGGTTGGGATAGGTGAGTTCTA1650.109723496788094No Hit
TCGTTGGGGCCGATCTCGAAACGGGTACAGAACAACGGTTTTTCTTCTGCCA1650.109723496788094No Hit
CTCTTGGGAGACATTGAAAATAAGGGACAACACCAGAAAAAACTGGTTAGCG1650.109723496788094No Hit
GCTCACGGGTCTAGAGAGTAAGGGTAACAGATTTACATAGAATAACAGCCTC1650.109723496788094No Hit
TGCATCGGGTATTTGTGTCATGTCTGCTTTCTTTCAAGAGTATTTTGTAGTT1650.109723496788094No Hit
ATCTACGGGACAGCCAGGGTATCCAGGATGCCAACCGTTGGGTCCCTACCCT1640.10905850589846919No Hit
AAAACGGGGTGATCCGCCCTCCTTGGCCTCCCAAAGTGCTGATATTACAGGT1640.10905850589846919No Hit
ATACGTGGGATGAGAAAATGGAATGTTTCAAAGTACAGCCAGTCATTGGGGA1640.10905850589846919No Hit
CACTTTGGGGGGGCTTCCTTCAGTGAAGCCCAACACACAAAACGTCCCTTTC1630.10839351500884437No Hit
TGCTTAGGGATTTCAAGTCTTCTATATGAACTTCTTGGAGCTGATTTTAGTT1630.10839351500884437No Hit
GGTTCCGGGTGAAAGGAGATGACCTTCAGGCCATTAAGAAGGAGCTGACCCA1620.10772852411921957No Hit
TTGTTGGGGGTTCTTCCTGCTGAAGTATTTATTGCAGAATAATAACAATAGA1620.10772852411921957No Hit
TTAGAAGGGTGGATATAAATGCCAGCACCTAGACCAAAGTTTTGTTTTGTTT1610.10706353322959476No Hit
GTAGCGGGGGGTTCCGGCGCGGCTGCTGGCCGGCGGCTGAGCCGGGAGAAAG1610.10706353322959476No Hit
CCAACAGGGTAGTGACATAGGAAAATGCTTAAGACTTAAAAAGGAAAGATTT1610.10706353322959476No Hit
ATATCTGGGGCTTATGTTATGTTTTGTGAATTGTGCTGTGTTGTAGACATTT1610.10706353322959476No Hit
TGTAACGGGTGAGTGCCCAGTGGATGGGTGCTATGGCAGCAGAGATTTTACA1600.10639854233996994No Hit
GCTATAGGGATAGAAGCAAACCCAGACAGGTTGAGTGACAGACATGTCCATC1590.10573355145034513No Hit
TACGCTGGGTCCCCATGGGGGCGGTAGCTGAAGTTGGCGAGCGCAGCGGTGG1590.10573355145034513No Hit
GTCACGGGGGGGATTGCAGGTCATCATCAGATTTGAAATATTTAAAGTGGAT1590.10573355145034513No Hit
GATTGCGGGCATCATCATCATCATCATCACCATATTTAGTATACATTCACTG1590.10573355145034513No Hit
ATTTTAGGGCTATTTTTTCTGGGAGAGGGGGGGATTGATTAGAAAAAAATGT1590.10573355145034513No Hit
TGACAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1580.10506856056072032No Hit
TCAGCTGGGAAGACACAGCAACTGTTCGATGATATTGAAGTATTCAAAATAT1580.10506856056072032No Hit
GACCCTCCCTCTATTAAGAGGGATTGGCCTGTTTTTGGAAGCTGGGCTGAGC1580.10506856056072032No Hit
AGTGCCGGGAGAATTGTTCCAAAAGAAAATAGGTCAGCTTTTAAAACAAAGC1570.1044035696710955No Hit
TCGGATGGGGGTTCACATTAGATAGTCAAGAAATGTAAATAAGAAGCTGAAC1570.1044035696710955No Hit
CATCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1570.1044035696710955No Hit
TTCAATGGGGGAAGGGGAAGGTGAGACATCTGAGTGAGCAGAGTGTGGCATC1570.1044035696710955No Hit
GATGGTGGGATCACCGGCCCCATCGCCAAACTCATCCATGAGCAGGAGCAGC1570.1044035696710955No Hit
GACCGCGGGGTTCTTATCTTTGAAATAAGAAGCTTAGATTGCATGAACCTGA1560.10373857878147069No Hit
ACCAACGGGAATCCCAGCTACTTTGGTGGCTGAGGCATGAGAATCACTTGAA1550.1030735878918459No Hit
TCAACTGGGGGAACCGCAGCGCGCCGCAGGACCGGGCCGCTGAGCCTGCAGC1540.10240859700222106No Hit
AGCGCGGGGGCTTCCGGCGGGAGCGGAGCACGGGGTGATCTGGGCACTAACG1540.10240859700222106No Hit
ACACATCCCCAAGGAGATCGGCCACGAGAAGCACCGGGACTGGGTCAGCTCT1540.10240859700222106No Hit
TCGACAGGGCTCTCGCCAGGCGTCCTCGTGGAAGTGACATCGTCTTTAAACC1540.10240859700222106No Hit
AGTTTGGGGCAGGTCTGCCTTTTTTTCTGGAGAATAAATGCAGTAATCCTCT1540.10240859700222106No Hit
GATTCTGGGCGAATTCCAGACCTCAGGTGATCCACCCGCCTCGGCCTCCCAA1540.10240859700222106No Hit
GCATTTGATTTTTTATTATTTTATGTTCCCCCGTTAGATTGTTTTAAGTGTT1540.10240859700222106No Hit
CAACGGGGCCGATCTCTATTAGGGGACAGGCCCTGGGAGGCTGCTGTGCTCT1530.10174360611259627No Hit
GGGCCGGGGACTTTTGGGTTTATTTCTTTTCTGTGTCAACAGGTCTTTTATG1520.10107861522297143No Hit
AGGAGGGTTGGAAACGCCAAGCCAGCTGCGTCCTAATCCAAAAGCCATGAAC1520.10107861522297143No Hit
AATCAAGGGAGTATGCAGAGACTTTCCTCATTAAAAAAATTATAAGTTTTTA1520.10107861522297143No Hit
ATGCCTGGGGGACTATTCCGCTTACCGTTCTTCCCTTCTCTTTTGGGCCCTC1520.10107861522297143No Hit
CAAGATGGGGGGCTTCAGTTCCATATTTGCCATGTGTCTGTCCAGATGTGGG1510.10041362433334663No Hit
TACGGTTGGGAGCAAATAAAATGCAGAATGCCAATTAAATTTGAATTTCAAA1510.10041362433334663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)