FastQCFastQC Report
Sat 31 Dec 2022
SRR5845178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151718
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT7480.4930199448977709No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC7210.47522377041616687No Hit
TTAGCCAGGGGTAAGAAACTGGTAATCATTTTTTCAAAGTACTTGTGCCACT3640.23991879671495803No Hit
ATCGTTGGGAGGCCCAGGGTGGCTGCTGCTTCCTGCTAAGTGGGGGAGGTGA2760.18191645025639672No Hit
ATCTTGGGGTAATTTAAATTTTAAAAACCTCCAAAAAACCAATTTCCAAGTA2550.16807498121514916No Hit
CTCTTCGGGGAAGAATCTTTAAGATAAATTGAAAGTGCAATTTCTGGTTACG2520.16609762849497095No Hit
TGTCACAGAAGAGGGGTAAACCATACACACAAATGAGAAAGAGGAAGGGCTC2220.14632410129318868No Hit
CTAAATGGGTTATTAATCCCACTACTAGATATCTACCCAGAGGAAAAGAAAT2210.14566498371979594No Hit
CACCAAGGGATTACAACTTGCTCTTGAGATAACTACCAACTCTGATGATAGG2210.14566498371979594No Hit
TTCACTGGGCAAATGAATTCCTTCGAAATGTATTTGAACTTGGACCCCCAGT2100.13841469041247578No Hit
ACGCTAGGGACAGCCACCCCCAGAGCAAGAGAATGTGCAGTATGCTGGCCTT2060.1357782201189048No Hit
GAGGCTGGGCCAGAGTCAAGCCCCAGCAACTACAGTGCTTTACAGGAAGTGT2050.13511910254551207No Hit
GCTGTGGGGGAGTTGGGGTTACAGCTGTTCAGAACAGGCTCAGGGCACATCC2040.13445998497211933No Hit
CAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2040.13445998497211933No Hit
GTTGTTGGGGCTCACCTAATGAAAAATAATGACATAATTTTCAGAACGCTTT2040.13445998497211933No Hit
GATCATGGGTACTCCAGCCATGCAGGGCGCCTCGAGCAGCGTGCTGGCTGAG2030.1338008673987266No Hit
TTTGCAGGGGACTGAGAAATTGGAACATCGATCAGTTAAAAAGGCTGGCATT2020.13314174982533383No Hit
CACTGCGGGTTACTGAAGATGCCAGAGTTTTGAAAACTGAATGTGATTCTTG1970.12984616195837012No Hit
GGTACAGGGAGTTTGTTTAGTTTTTTCTTACTTTTAAATTCCAACTTAAAAT1960.12918704438497738No Hit
GCTTGGGAAGACATGTGTGGACGAGATGCAGAAGATTACGCTATTTCTTGCC1930.12720969166479915No Hit
TTCTGTGGGTGTGGCCAGAAACTCCTGCCTTGGGATTTGCCGAATACTAACT1920.1265505740914064No Hit
AGCAACGGGCACGATCAACACCAACCTGGGTGACCTGCAGTTCCTGGCCATC1900.12523233894462096No Hit
TTAGCCGGGATCTAGAGGAGGGATCCTCATCGACATGGTCCTGAACCGCATG1880.12391410379783546No Hit
GAGTATGGGTGAAATTAACTCAGTAGAAGGGCTTAAGAACAGATTTGGCCTA1880.12391410379783546No Hit
TCGTTGGGGCCGATCTCGAAACGGGTACAGAACAACGGTTTTTCTTCTGCCA1860.12259586865104997No Hit
AAAGCAGGGTAAATATGGAAAAATATTAAATCGATTGAAAGCATGTTTTCAG1850.12193675107765724No Hit
CTTGCCGGGAACAGATAAATACATTACACCTTTCAAAGAATAAATGAAAAAT1850.12193675107765724No Hit
AAATCTGGGTTATAGGTGCTTAGTAAATTTTTGTTGAATGAAAGATGACAAA1840.12127763350426449No Hit
AGTCTGGGGGTATCCTGTCAGACACGTCGGACGAGGAGGACGCGAGTGTCAC1830.12061851593087175No Hit
AAAGATGGGGGGATATAATGATTATATCCTACACTTCTGCATTACTCCACAG1830.12061851593087175No Hit
GGTTCCGGGTGAAAGGAGATGACCTTCAGGCCATTAAGAAGGAGCTGACCCA1820.11995939835747901No Hit
TACAGTGGGAGCGCAGAAGCCTCTGCTGTCCTTGGAGGAGACTCTGAATGTG1810.11930028078408626No Hit
TGTGCAGGGTGAAAATGCAATTCAGACATGATGATCCAGTTCCCCATCACAC1800.11864116321069353No Hit
GGCATGGGGAGTCTCAATTGGGCATATCTAAGTTACTGGCCTTAAAGAGGAG1800.11864116321069353No Hit
TGATTCGGGAGTTCAAGAAAATGAGGAGAAAAGGCTACAGCTTCAGAATGCG1790.11798204563730078No Hit
AGGCTCGGGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAA1790.11798204563730078No Hit
GCTACAGGGACCCATTTTTGTGGGTGAGGAACTGGAAAGGGATGGATTTCCT1770.11666381049051529No Hit
GCGACCCCAGCAGTGCCAGTCAGTTCTGAGCGAGCGGCCCCTGGACAGTTGC1770.11666381049051529No Hit
AACTCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1760.11600469291712255No Hit
ATGTGGGGTTCAGCATGACCAAACACTGGGGGGTTGGGATAGGTGAGTTCTA1760.11600469291712255No Hit
AAGCACGGGGATCTTTGAGCCTAGGATGCTAAGGTTGCAGTGAGCCAAGATG1760.11600469291712255No Hit
TTGAACGGGACATCATCATCTACGCCTGGTCCATCGGCGGCTTCACTGCCAC1750.1153455753437298No Hit
GATGGTGGGATCACCGGCCCCATCGCCAAACTCATCCATGAGCAGGAGCAGC1750.1153455753437298No Hit
TTAAGCGGGAAGAAAGACTTTAGTAGTAAGGAGAATTCCACCAGGGTACAAA1740.11468645777033706No Hit
GTCACGGGGGGGATTGCAGGTCATCATCAGATTTGAAATATTTAAAGTGGAT1740.11468645777033706No Hit
CTGCCTTAGTTGCATATCATCATGCTTATTGTTAAAAGTTAAAATGCATACA1740.11468645777033706No Hit
ATACGTGGGATGAGAAAATGGAATGTTTCAAAGTACAGCCAGTCATTGGGGA1740.11468645777033706No Hit
GCATTAGGGACGGAACCAAAAACAAACTCTCCAAGTATATTCACACATTCAA1740.11468645777033706No Hit
GTATAAGGGTTGTGTAGTAACACAAAGCTGCAGGTTTTAATTTTGCTTTGTC1730.11402734019694433No Hit
GCGGTTGGGAACAGAAAATTTCCTGCAAGCTCCCCTAAGCTTGAAGAGACAA1730.11402734019694433No Hit
TTAAAAGGGGTTTAAACAAAATAGATACAAGCTCTGGCTTCTAGACACCTGC1720.11336822262355158No Hit
ACCGTTGGGTGACATTAACATATGAAAAAACAGCTACTTGTGCCTGACTATG1720.11336822262355158No Hit
GCCTCCTCATCCTTATTATCAGCACTCAGGCGCCCACTGTCTGCTCGAACTG1720.11336822262355158No Hit
TGAATAGGGATTGTCGCCAAAGTTTGAGAAGCACTCAGGCTTTATGCTCCTT1710.11270910505015885No Hit
GCACGGGTTCCCCAGGCATATTTCAAAATGGTTACTTTTCTCCTACCCCTGC1690.11139086990337337No Hit
GAAATAGGGCTCCTGAGCTCAGGCAGTCTGCCTGCCTTGACCTCCCAAAGTG1690.11139086990337337No Hit
TATTTTGGGCAGGCAGATGAGTCCCTAGCTTAAACAAGAAAATGAACTATGC1690.11139086990337337No Hit
TAAACAGGGATATATATCACGTTACCAAAAAAGAAGGTGCAGAAAAATTGAT1680.11073175232998063No Hit
TTTGCTGGGATCTTACTCTAGGTTTGTAAAGTTCCCTATTATTATTTCTTTG1680.11073175232998063No Hit
TAAAGGGGTGTTACTTAGGCTGTTAGATCCAAGTTGATTTTTGTGTCTACAG1670.11007263475658789No Hit
CATCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1670.11007263475658789No Hit
TGACAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1670.11007263475658789No Hit
ATGCCTGGGGGACTATTCCGCTTACCGTTCTTCCCTTCTCTTTTGGGCCCTC1670.11007263475658789No Hit
ATACTAGGGTCTAGGAATGGGGCAAAAGAATGTATGGCTGGAACTTCAGGGT1670.11007263475658789No Hit
GCATTTGATTTTTTATTATTTTATGTTCCCCCGTTAGATTGTTTTAAGTGTT1670.11007263475658789No Hit
TTGCAAGGGATCCAAGATCAATTCCCTCTCTCAGCACAGCCTGGGGAGGGGG1650.1087543996098024No Hit
CGGATTGGGGTAACTGGTGGCTATCCATTTGAAGCTTACAAAAATTGTTTTC1650.1087543996098024No Hit
TGCGATGGGATTTTATCATAATTGCAAATCCTTTTCAATTATATATGCAGTT1650.1087543996098024No Hit
TTTGGGGGGGAGGGTCATCTTTTCAGAGAAATTTAAGGATTAAGACAGCATC1650.1087543996098024No Hit
GCAATAGGGGGAAATAGAACCCTTGGCTTTTGTGTGGCAAGAGCAATGGAAA1640.10809528203640965No Hit
GACCGCGGGGTTCTTATCTTTGAAATAAGAAGCTTAGATTGCATGAACCTGA1640.10809528203640965No Hit
CACGTTGGGGAGTTCATCTGCGCTCTGTCCATCACCTCCAGGGGCAGCTTTG1640.10809528203640965No Hit
CTCCAGGGAAGGAAGGGGGACGATGGGAAATCATGGACTTGTAAGTTGTATT1640.10809528203640965No Hit
GACCTGGGGTGATGGGATCACATGGCAGCATGTACCTGATGCAAGGTACTGC1640.10809528203640965No Hit
ATAGTAGGGGTGGCACAGGTTATAAACTTAAGTGGAGTTTACCGGCAGCATC1640.10809528203640965No Hit
ACAAACGGGCTATAAGTTCTAGAAATCTAATAAATAATGCCAAATTTTTAGC1640.10809528203640965No Hit
AATCCAGGGAGATAAGGCTAACCTATGCTCTTATGATCTGGGTGAGATTGCA1620.10677704688962417No Hit
GCATTCGGGGTTGACTTTGTATCTCTGCCTGTGATTTTCTTTTCTAAATGAA1620.10677704688962417No Hit
GATTAAGGGATTCAGCTCCAGTGTGTGCCCCATACTGAAACTTTCCTTATTG1620.10677704688962417No Hit
TCAGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1610.10611792931623143No Hit
TCTTCGGGGCCCTATTTTCAGCTACTATTTGAATATTCTTGAACACCACCAC1610.10611792931623143No Hit
ATGCATGGATCTACTTTTTCTTAACCCAAGTGATAATAAACAATATTCACAA1610.10611792931623143No Hit
GCAGTAGGGCCTAAAAAATAGGAAACTATCGTCTCCATTTTACATATGACAA1600.10545881174283869No Hit
AGATTTGGGGAAAAGCCAGGTCATATTAGAAGATTACACGAGTCTTGGCTTG1600.10545881174283869No Hit
TCAACTGGGGGAACCGCAGCGCGCCGCAGGACCGGGCCGCTGAGCCTGCAGC1600.10545881174283869No Hit
GTAAATGGGGTTTGTCATAATTTTGAAATCTATCTTTTTTCTCAAGTCGCGT1600.10545881174283869No Hit
AGATTAGGGCAGCCTGGGCACAATCTAGCCTGGGCAACAAAGGCGAAACTCC1600.10545881174283869No Hit
ATTTTAGGGCTATTTTTTCTGGGAGAGGGGGGGATTGATTAGAAAAAAATGT1600.10545881174283869No Hit
AGCCTGGGCAACGGAGTGAAACTCGTCTCAAAAACCAAAACCAAAACAAAAC1600.10545881174283869No Hit
ATATCTGGGGCTTATGTTATGTTTTGTGAATTGTGCTGTGTTGTAGACATTT1600.10545881174283869No Hit
AGGAGGGTTGGAAACGCCAAGCCAGCTGCGTCCTAATCCAAAAGCCATGAAC1590.10479969416944594No Hit
TGACTAGGGTGTGATACTCAGTTTTGTAGACTGCAGACCATAACCAGCAATT1590.10479969416944594No Hit
ATCTAGGGGACTTGCGCACGCTGATCAACGAGCTAGTGAAAGGGATCTTGCC1590.10479969416944594No Hit
AAACATGGGGGGTCTGTTGCTCTTATTGGCTTCCAAATGTGCATGGCAAAGA1590.10479969416944594No Hit
ACAGTTGGGGGAGGAACTGATATAGTTTGCTGGTGAAGTTAATTAACTTTTA1590.10479969416944594No Hit
GGAATGGGAACAGACAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCAC1590.10479969416944594No Hit
GCTATAGGGATAGAAGCAAACCCAGACAGGTTGAGTGACAGACATGTCCATC1580.1041405765960532No Hit
TGTAACGGGTGAGTGCCCAGTGGATGGGTGCTATGGCAGCAGAGATTTTACA1580.1041405765960532No Hit
ATAGCTGGGGACATAAGTGTTAACCAATCACTCTCTCTCTTTACAGATACTA1580.1041405765960532No Hit
TGCATCGGGTATTTGTGTCATGTCTGCTTTCTTTCAAGAGTATTTTGTAGTT1580.1041405765960532No Hit
GCACAAGGGATAAAATATTGAGTGTGAGATGATATGTAGTCACTTTACTTTT1580.1041405765960532No Hit
GGTCACGGGCATTGCAGCAAGGATGTAGAGAGAAATAGGACTTAATTCCACT1570.10348145902266045No Hit
CATGTCGGGTGCCCTTTGTTGAAATGTCTTTTGCCCATTTTCCAATTGGATT1570.10348145902266045No Hit
CTCTTGGGAGACATTGAAAATAAGGGACAACACCAGAAAAAACTGGTTAGCG1570.10348145902266045No Hit
AGTCGGGGGCCCTTGTTTAGGGAGAACAAAACAACACATCCAAAAAAATGAC1570.10348145902266045No Hit
TCAACAGGGGTTTACTTGTAACTTTCTGGTTATATACTGCTTATATCTGTGG1560.10282234144926772No Hit
GCCTCTGTGGCGGCGATCTATCACAGGGCTGGCAGTGTGCAGGAGTGGGGTT1560.10282234144926772No Hit
TGTTGCGGGATTTGGGAATTATTAAAAAGGCAAACAATGAATGTTATTAGGA1560.10282234144926772No Hit
GCTTGAGGGTTATTCAGTTATTCAAGTATCCAGTTATTTAGTTTCAAGGTAG1560.10282234144926772No Hit
AATCAAGGGAGTATGCAGAGACTTTCCTCATTAAAAAAATTATAAGTTTTTA1560.10282234144926772No Hit
AGACTAGGGAATTATCTTTTAGCCTTCCTCCTAATTTTCTAATTGAAACTTC1560.10282234144926772No Hit
TGCGTTGGGTACCTGGGAGTGGAATTGCTAATGTCTTATGGTAAGTGTGCAT1560.10282234144926772No Hit
TCTACGGGGTAGGATACGATACTTAATTTTACGCTTCCTTATTTCTTAGGTG1550.10216322387587497No Hit
TGCTTAGGGATTTCAAGTCTTCTATATGAACTTCTTGGAGCTGATTTTAGTT1550.10216322387587497No Hit
GCGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1540.10150410630248223No Hit
ACACTAGGGTTCACGCAGTGGCCAATAATCAAGACAAACTGGGATTTGAGGA1540.10150410630248223No Hit
GACAATGGGCAATCTGGGTACTGGTGAAGGTTGTTTGATAATAAAGACACAG1540.10150410630248223No Hit
TTAACTGGGCAGGATCAGAGGATGTACCCCATTCCCGCTACCCTTAGGTCTA1540.10150410630248223No Hit
TCCGCGGGGATGTGATAAAAGCAGGATTCAGCTGGACAGGCACAGATATTAT1540.10150410630248223No Hit
GCTTCTGGGGGGAACAGGGCATGCTAGAGGTGGGTCATGAAGCTTTCAAGGT1540.10150410630248223No Hit
CACGCCGGGTTAGCATAACTTTGGGTGACTTTAACATTTCTCAGACTGTTAT1540.10150410630248223No Hit
ATCGCTGGGTACATCAGACAAACTTTAAAGCAACAGCAGTGAAAAATAACAA1530.10084498872908948No Hit
GAACTGGGGCATTGTTCACTTTATTATTGAACTTTCTTTATACTTTCATGTG1530.10084498872908948No Hit
CTAATTGGGGTGAAAAAGATAAATATGTTTAGAATCTCTGATACCTGTGCCT1520.10018587115569674No Hit
CAACGGGGCCGATCTCTATTAGGGGACAGGCCCTGGGAGGCTGCTGTGCTCT1520.10018587115569674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)