FastQCFastQC Report
Sat 31 Dec 2022
SRR5845159.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845159.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359042
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC11850.3300449529581498No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT9930.2765693150105002No Hit
TTAACTGGGCTATCGGCGGGGTCAGAGTGCTCCCAACTTGCTGTAAATATTT4540.12644760223037974No Hit
GTAATGGGGAGGGAACCTGGAAAAACCTTTGAAAAGTAAATTATCAGTTTAG4480.12477648854451569No Hit
GCAAAAGGGCAGCTTCCTAGTGGGAAGTAAAACTCATAGCAATTCTCTCAGT4390.12226981801571962No Hit
AGGCGGGGCTTCTCAAAAGAAGACATACAAGTGGCCGACAAACCCATGAAAA4350.12115574222514357No Hit
GCACTGGCCCCGGGAGGAGTCTGACCAAAAATGGAGCCCGCTCTGTGGGAAG4230.11781351485341547No Hit
CAGTCCGGGCACCACAGGATGCCTGCCATTCATGAACAAGAAGGAGAGGACG4210.11725647695812746No Hit
TGCTCGGGAAGACTACTTCCAAAAGCCCTGCTTTGTTCTGCAAGTGCCCAGC4150.11558536327226342No Hit
AAGAATGGGGAGGGGCTGGGTGGCGGTTGAGACAGCGGCGGTACTGGGAGGC4140.1153068443246194No Hit
AAGTGAGGGACTGTGAGCTTGATTTCTGTAGTCCCTGTCTCTAAAATTGGGG4140.1153068443246194No Hit
AATTCGGGGGCTTATCAGATGATTTTCTCTCAGTTTCTGTTGGCTAGGATTG4050.11280017379582334No Hit
TCAATTGGGATATTGAAACCTCTGACATTCTTTTACCAGCAGTTGTACTGCC3990.1111290601099593No Hit
GATTATGGGCTTCATGATTCTCTTCTGAATCCATAAATCCTTTTCCCTAAGG3850.10722979484294316No Hit
GCTACCGGGACTTAGAGATTTGAGGAAATTTTTCCTTAAAGATTTTCAAGTT3850.10722979484294316No Hit
CAGAACGGGGGGGAGTGTTTGTGTCTGACCACAGACTAATTTGAGTGAAAAC3790.10555868115707912No Hit
TTCATTGGGAGGTCACACGTAGACTTGAAGGATGAATGTGGGAGTTTTATTG3780.1052801622094351No Hit
TGGTGGGCTCTTGTGGGGACCTCTGGTCTGGGCTGCCTTTGTCAGGAGCCTG3780.1052801622094351No Hit
GGTGTGGGGGGTCAGTGATGTCATTGAGTACAGAACCGATTTCAATCCCTCA3710.10333052957592706No Hit
GCCAAAGGGAACCAAAGTTCGCTTTGAAGAATTGCTTAAGACCCACAGTGAT3710.10333052957592706No Hit
GGCGCTGGAGGGATGGTTGTAAAGTAGATAGATCATTGGTTCAACCATTTTA3700.10305201062828305No Hit
GAACTTGGGGGATAGAATGGAGAGGTTCTTAGGAAATAAAATCGCCAGAACT3680.10249497273299502No Hit
ACCTCAGGGATTTTCATTGCCTGAAAAATTAGCCAACGCTTGGGGGGAAATG3630.10110237799477498No Hit
TGCATGGGGAAGCAATGGAAATGCCCATTTCCATTGTTGTCTCCAGTTGCTC3630.10110237799477498No Hit
TCTCAGGGGCGATCTTCAAATGGGATGATGGTTAAGACAAAAACTGAGTTAT3620.10082385904713097No Hit
CCGATGCGGGATGTGTCAAAAAAACTCTAAAAGGTTAGCTTCATCGCGCAGG3610.10054534009948696No Hit
TAAGCTGGGATTACAGGTGTGAACCACTGTGCTCAGTCTAATAAATGCCTAT3610.10054534009948696No Hit
CTGTAACAGGGATTGTTACACTATTACTCAATGAGAATATTGCACCTGTCAG3610.10054534009948696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)