FastQCFastQC Report
Sat 31 Dec 2022
SRR5845158.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845158.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358707
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC11650.32477760400549754No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT10000.2787790592321867No Hit
GCAAAAGGGCAGCTTCCTAGTGGGAAGTAAAACTCATAGCAATTCTCTCAGT4660.12991104160219902No Hit
TTAACTGGGCTATCGGCGGGGTCAGAGTGCTCCCAACTTGCTGTAAATATTT4530.12628691383218058No Hit
AAGAATGGGGAGGGGCTGGGTGGCGGTTGAGACAGCGGCGGTACTGGGAGGC4470.12461423947678746No Hit
GGCGCTGGAGGGATGGTTGTAAAGTAGATAGATCATTGGTTCAACCATTTTA4450.1240566813583231No Hit
TCAATTGGGATATTGAAACCTCTGACATTCTTTTACCAGCAGTTGTACTGCC4420.12322034418062652No Hit
GCACTGGCCCCGGGAGGAGTCTGACCAAAAATGGAGCCCGCTCTGTGGGAAG4260.11875987923291155No Hit
CAGTCCGGGCACCACAGGATGCCTGCCATTCATGAACAAGAAGGAGAGGACG4250.11848110017367934No Hit
TTGAAAGGGTGGCCACGGGGGGACCCAGAGTTAGCAGTGGGGATGGAGCAGT4180.11652964675905404No Hit
GCTACCGGGACTTAGAGATTTGAGGAAATTTTTCCTTAAAGATTTTCAAGTT4120.11485697240366093No Hit
TGGTGGGCTCTTGTGGGGACCTCTGGTCTGGGCTGCCTTTGTCAGGAGCCTG4120.11485697240366093No Hit
TTCATTGGGAGGTCACACGTAGACTTGAAGGATGAATGTGGGAGTTTTATTG4110.11457819334442873No Hit
GTAATGGGGAGGGAACCTGGAAAAACCTTTGAAAAGTAAATTATCAGTTTAG4070.11346307710749998No Hit
AAGTGAGGGACTGTGAGCTTGATTTCTGTAGTCCCTGTCTCTAAAATTGGGG4070.11346307710749998No Hit
TGCTATGGGAAGTGAAATGACTAGTGATTTGGGCCCAAACCAGAATCTTTTT4020.11206918181133907No Hit
TGCATGGGGAAGCAATGGAAATGCCCATTTCCATTGTTGTCTCCAGTTGCTC3950.11011772839671376No Hit
GATTATGGGCTTCATGATTCTCTTCTGAATCCATAAATCCTTTTCCCTAAGG3940.10983894933748156No Hit
GTACCAGGGGAAATTTCTGTGGGAAAATTCACCTATTTTTAAAATAAAATAC3930.10956017027824938No Hit
TGCTCGGGAAGACTACTTCCAAAAGCCCTGCTTTGTTCTGCAAGTGCCCAGC3930.10956017027824938No Hit
AGGCGGGGCTTCTCAAAAGAAGACATACAAGTGGCCGACAAACCCATGAAAA3900.10872383310055282No Hit
GTTGCGGGGGTGCCTCCATTCCCTGTTAATAAAAGACTGATCTGTTCTTGAG3900.10872383310055282No Hit
TAGCTCGGGAGGAGTAGAAAGTGAAAGATAACTTGCCAATTGATTTGAAAAC3880.10816627498208843No Hit
TGCTTGGGGAAGACACTTTATATAATTTCCATTTTCTTAAATTCATTGAGAC3840.1070511587451597No Hit
GGTGTGGGGGGTCAGTGATGTCATTGAGTACAGAACCGATTTCAATCCCTCA3800.10593604250823095No Hit
GTAACGGGGCTTGCATAGGTAAGATAACTGCTGTAAAACAAATTTTAAACAA3790.10565726344899877No Hit
TCTCAGGGGCGATCTTCAAATGGGATGATGGTTAAGACAAAAACTGAGTTAT3790.10565726344899877No Hit
AGCTCAGGGTGATGTAATTTCTGTTTCTGTTTCCATCTAAACTTCTTTATAA3780.10537848438976659No Hit
CCGATGCGGGATGTGTCAAAAAAACTCTAAAAGGTTAGCTTCATCGCGCAGG3750.10454214721207002No Hit
GAACTTGGGGGATAGAATGGAGAGGTTCTTAGGAAATAAAATCGCCAGAACT3740.10426336815283782No Hit
CTGTAACAGGGATTGTTACACTATTACTCAATGAGAATATTGCACCTGTCAG3730.10398458909360564No Hit
TAAGCTGGGATTACAGGTGTGAACCACTGTGCTCAGTCTAATAAATGCCTAT3710.10342703097514128No Hit
CATGAGGGATTGACAGAAAACATCCATTTCCTCACATTTTCAAACACCAAGC3700.1031482519159091No Hit
GCAAAAGGGACAGAGGTGTCCACAAAAAACTGAAGAAAACAACCAGCTGTGT3700.1031482519159091No Hit
CAGAACGGGGGGGAGTGTTTGTGTCTGACCACAGACTAATTTGAGTGAAAAC3660.10203313567898034No Hit
ACTCAAGGGAAGCATCTGAAGTTTTAGAGCCCAATCTTTCATTTCCTTTCCC3650.10175435661974816No Hit
ACCTCAGGGATTTTCATTGCCTGAAAAATTAGCCAACGCTTGGGGGGAAATG3610.1006392403828194No Hit
GTGATGGGAGAGTGCTAATGGGTGGCTGGGGATACGGTAAATTATCTGTGCA3610.1006392403828194No Hit
AATTCGGGGGCTTATCAGATGATTTTCTCTCAGTTTCTGTTGGCTAGGATTG3600.10036046132358722No Hit
GCCAAAGGGAACCAAAGTTCGCTTTGAAGAATTGCTTAAGACCCACAGTGAT3590.10008168226435504No Hit
TACACAGGGGATTGGTCATTCAGAATCATGTTATTTAATTTCCATTTATTTG3590.10008168226435504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)