FastQCFastQC Report
Sat 31 Dec 2022
SRR5845157.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845157.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358081
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC10970.3063552659873045No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT9060.2530153792019124No Hit
GCAAAAGGGCAGCTTCCTAGTGGGAAGTAAAACTCATAGCAATTCTCTCAGT4570.12762475529279688No Hit
AGGCGGGGCTTCTCAAAAGAAGACATACAAGTGGCCGACAAACCCATGAAAA4370.12203942683359352No Hit
TTAACTGGGCTATCGGCGGGGTCAGAGTGCTCCCAACTTGCTGTAAATATTT4330.12092236114175285No Hit
TGCTCGGGAAGACTACTTCCAAAAGCCCTGCTTTGTTCTGCAAGTGCCCAGC4120.11505776625958931No Hit
AATTCGGGGGCTTATCAGATGATTTTCTCTCAGTTTCTGTTGGCTAGGATTG4070.11366143414478846No Hit
TCTCAGGGGCGATCTTCAAATGGGATGATGGTTAAGACAAAAACTGAGTTAT3970.11086876991518678No Hit
GCACTGGCCCCGGGAGGAGTCTGACCAAAAATGGAGCCCGCTCTGTGGGAAG3960.11058950349222663No Hit
CAGTCCGGGCACCACAGGATGCCTGCCATTCATGAACAAGAAGGAGAGGACG3920.10947243780038594No Hit
TAGCTCGGGAGGAGTAGAAAGTGAAAGATAACTTGCCAATTGATTTGAAAAC3900.10891390495446562No Hit
TCAATTGGGATATTGAAACCTCTGACATTCTTTTACCAGCAGTTGTACTGCC3880.10835537210854528No Hit
GGCGCTGGAGGGATGGTTGTAAAGTAGATAGATCATTGGTTCAACCATTTTA3850.10751757283966477No Hit
CCGATGCGGGATGTGTCAAAAAAACTCTAAAAGGTTAGCTTCATCGCGCAGG3830.10695903999374443No Hit
ACCTCAGGGATTTTCATTGCCTGAAAAATTAGCCAACGCTTGGGGGGAAATG3830.10695903999374443No Hit
GTAATGGGGAGGGAACCTGGAAAAACCTTTGAAAAGTAAATTATCAGTTTAG3830.10695903999374443No Hit
TACACAGGGGATTGGTCATTCAGAATCATGTTATTTAATTTCCATTTATTTG3820.10667977357078427No Hit
GCTACCGGGACTTAGAGATTTGAGGAAATTTTTCCTTAAAGATTTTCAAGTT3800.10612124072486392No Hit
TTGTGCGGGCCTCCTTTGAATTCCTAATTGGTCAGCATACATTTATTAGCAA3740.10444564218710292No Hit
GTGCAAGGGACTGACCTTTGATCTAGTTTGACCTTTGAAATTTGCATGTGAC3650.10193224438046139No Hit
TGCTATGGGAAGTGAAATGACTAGTGATTTGGGCCCAAACCAGAATCTTTTT3640.10165297795750124No Hit
GAACTTGGGGGATAGAATGGAGAGGTTCTTAGGAAATAAAATCGCCAGAACT3630.10137371153454107No Hit
GCACGAGGGAATGACAATGAGATGGGAATATCCCCCGTCTTTATAAGTGAAT3600.10053591226566057No Hit
CTCACAGGCAAGATTAAAAAGAAATTCTGCTCCTTTTAGTAATAAACAATGT3600.10053591226566057No Hit
GATTATGGGCTTCATGATTCTCTTCTGAATCCATAAATCCTTTTCCCTAAGG3600.10053591226566057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)