FastQCFastQC Report
Sat 31 Dec 2022
SRR5845155.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845155.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139581
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT16861.2079007887893052No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC15421.1047348851204677No Hit
GTAAGCGGGGTTTGGCTCTGTGTCCCCACTAAAATCTCATCTCAAATTGTAA3180.22782470393534937No Hit
AAGAAGGGATTACACCAACATTTATTTAAACAGAATCTATAAAAATCATATG2670.19128677971930277No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATT2270.16262958425573681No Hit
CAGCCTGGGTGACAGAGTAAGACCCTGTCTTAAACAAACAAAAAATTAAAAA2200.15761457504961277No Hit
AGTTTTGGGGCTTGATGGGGATGGCATTGAATCTGTAAATTACCTTGGGCAG2160.15474885550325618No Hit
CTGGTTGGGTACATATTTCATTCATTTTGCCAGCATACCATATAGACAAGTT2150.15403242561666702No Hit
AATAGTGGGCAGCCTGGTTTTAATGATGTTGATATGTTTGACAGGTCTCATT2140.15331599573007787No Hit
TTTGTAGGGGCCTGAATTTGATACGCATGCAATGAGTACTTTTACAAGAAAA2120.15188313595689956No Hit
TCACGTGGGGGAAATGTACATCTCTAGCCATCAACTAGTAACAAGAATGGCG2110.15116670607031044No Hit
CTCTTGGGGGAGGAAAATTATAAGGTAAAATTTTCCTGTTTTTCCCCCTTTG2020.1447188370910081No Hit
ACTAGAGGGTACCACTGCCTCATACTTTCTTCTACATCAGAGAATTCATAAA2010.14400240720441895No Hit
CTCTCAGGGACCCACAAATCAGGAGTGAGATGAATGTCGTGATTATTTTTTG2010.14400240720441895No Hit
TATATTGGGCAGTCCCAGAATTGGCTTAGCACAGAGACTCTAAATGATAGTA1980.1418531175446515No Hit
TGCTGGGGGAGGGAAAATAATACAGAGTTTAACATTTTTAGATTGAAAGACA1970.14113668765806234No Hit
TTAACGGGGTAGGCAAGGGACTCTTGGTCTCCTACTTATCCCAGGGAGCCCT1960.14042025777147318No Hit
ACTTATGGGAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGA1960.14042025777147318No Hit
TTTTGCGGGCATTTATAATTCCAGTATTTGTTGTTATCTAGAAAATGGCTGA1940.1389873979982949No Hit
AGCATGGGGGATGATACAAATATTTCCATAATCTTGAGACTACAAAGCTATA1930.13827096811170575No Hit
TAAGCTGGGAGTATCAATTATTTCTATATTTACCAAAACACCTACTACTTTA1920.1375545382251166No Hit
GATTCAGGGATATGTCTGTAGAATTTTCATCTGTGTTAGCTGAATGAGCTAC1900.1361216784519383No Hit
CTTGCAGGGGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTT1900.1361216784519383No Hit
ACGCTGGGGGATAGTATCTCACATCTCAATATTAATGTTGAATGTAAATGAC1890.13540524856534916No Hit
TGTTTGGGAACTGTAAAATAAATACATTTGAAAAAGGCACCCTCTCAACCTT1880.13468881867876No Hit
GATCCGGGGTAGCCAAGGTTAAGAACCAGTACCGTACGTACAAAAAAGTTGA1870.13397238879217085No Hit
TATGCAGGGACCACCAAAGTTACTATGAAGCCTGCTGGGTTTGGAAACCTAG1840.1318230991324034No Hit
TACGCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1820.1303902393592251No Hit
CTTACGGGTTAGGGTGGAATGAAAAAACCAAACAACAAGCAAGGGGATTTAT1810.12967380947263596No Hit
CTTGTGGGGGGCATCAGCTAAATCTCTGTCATATTGTTTATATTTTTTGCAA1800.1289573795860468No Hit
GCTTCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1800.1289573795860468No Hit
AGCCGGGGGAAGTAAAAAAGATTTTTTAAAATTTTGATGAAGCCCAATTATG1790.12824094969945765No Hit
CCAACCGGGCCTCCCGGGTTCGGGAGATTCCTCTGCCTCGGCCTCCTGAGTG1790.12824094969945765No Hit
AAAACCGGGCCTGGCATACATTATATATTAGTGAATATTCTTATTTGAAAGC1780.1275245198128685No Hit
AGGCTGGGTGACTTGAATCCAGGAAGCCCATTTTCACACTAGCCCTCAGGCC1770.12680808992627934No Hit
TGTTCTGGGACCTCGTGATCTGCCCACCTTGGCTTCCCAAAGTGCTGGGATT1760.12609166003969022No Hit
GCTTGTGGGCGATCTTGCAAGGGGATCTAAAGAAGGGCTTGGTGGAAGGAAG1760.12609166003969022No Hit
TCCGTGGGGGGTGTGACTTTGTTCCAGTAAACTTTCTTTATAGAATGGGAGA1760.12609166003969022No Hit
AGTCAAGGGTGTGGCTTGAATGCCTTCCCCTCATCTTGCCATGCTTGGCTGT1760.12609166003969022No Hit
ATGTAGGGGAGGGAAGTGTCTTACCTATTGCTTTTTTGCAGAGGATATTTTA1750.12537523015310106No Hit
GAACTAGGGTGAAGGTAGAGGTGACATAAAAGTAGGCTGTATATTCAAATCC1750.12537523015310106No Hit
GAGCAAGGGCAGCTCACCACAACTTCCGTCTCCCAGGTTCAAGTGATTCTCC1740.12465880026651191No Hit
ATGCTAGGGTCCCTCAGCTTAAAAAAATGGGACTCCTAGGGCTATTTGCCTT1740.12465880026651191No Hit
CATCCAGGGGATCCCGCGGTCCCAGGACCCCCGGCAGCAGCTGCCACGCTCA1740.12465880026651191No Hit
ATTTCAGGGCTGGGAAGAACTTAGCAAGGCCTGTTTGCTCAGATTTTTCTCT1740.12465880026651191No Hit
GACAGCGGGGACTGCTGTAAGGACTGAATGGGCACAGAGGCTGTGACTGCTA1730.12394237037992278No Hit
GGCGATGGGCCTGGAACTTGAGTTATTAAAATCATAGGAATAAAATTATGGG1730.12394237037992278No Hit
AAGTACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1730.12394237037992278No Hit
GGACTGGGGATAGCAAACGCTTTATAATTGGCATAGACATAAAGGATAAAAG1730.12394237037992278No Hit
TGCCTTGGGGGAAACTTTCACAATGTCCGGAGCCCTTGATGTCCTGCAAATG1720.12322594049333363No Hit
TTAAAAGGGCTCCTCACTCACCTCCAAGCTGATCCGTTCGCACCCAACCACT1700.12179308072015534No Hit
GCCACCGGGTGCGAAACTCGGCGGCCACGGCCGAGAAGTGGCGCTTCCGCCT1700.12179308072015534No Hit
TAGCGTGGGATCTGCTACTGCCCTTTCTATAATAACTAAAGTTAGCTGCCCT1700.12179308072015534No Hit
CTTCAAGGGCAAATCCATCACACATTGGCTTATTTGAAACTTTTGGTTTTCC1700.12179308072015534No Hit
ATCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1690.12107665083356618No Hit
ACATGCGGGATTGTCAACTATGATTACAAAGTAGTCCATTCTCAAGGTAAAC1690.12107665083356618No Hit
CACTGTGGGAACACCACTCATGCAACATTCCAGCCAGGCATGGATAACCCAA1690.12107665083356618No Hit
GATACAGGGATTTGAGGACATGGTGTTTTGTAACCTGTTGTTTTTTCTTAAT1680.12036022094697703No Hit
ACAAAAGGGCCACCTCACCCTCTCCGCACACCTCTGGCTGTCTTTTTGTACT1680.12036022094697703No Hit
TTCATAGGGGGGATCGTGACAAAACATACAGGAGCTGATAACCAAAATAGCC1680.12036022094697703No Hit
GCCGCAGGGGAATTCGAGGAAGATGATGTGAATCTTCCAATTTTATCTATTT1670.11964379106038789No Hit
GTACTAGGGGTGGCCACAGTCAGTTTCTTCTGGGAGGAGAGCTTGACCCAGC1670.11964379106038789No Hit
ACCAAGGGGCTGGTAGGGCCTGCAAACCTGCCCTACCAAACCTGAAAGAGGG1670.11964379106038789No Hit
CACCTAGGGATGTATAGGATTCATAAGAACTACAATGATTACATCAACACCC1660.11892736117379873No Hit
AATGCTGGGATAATAGAATAGCCAAATGCATACTCTTCTTCTCACTAGAAGG1660.11892736117379873No Hit
GGTGCAGGGATCATATATCATATATGAATGTACATTAGTCAAATGGTGTGAG1650.11821093128720958No Hit
TTGCAGAGGCATAGCCTGTAAGTACATTCTGGGTGACCTTGTCTTGTAGGAC1640.11749450140062043No Hit
TTCTATGGGGGGGCCCCTGATGCCAAGAGCTCTGGAGGTTCTTGAAAGAGGG1630.11677807151403129No Hit
CAGCCTTCCCAAAGTGCTGGCATTGTAGGTGTGAGCTACTGCACCTGGCCAA1630.11677807151403129No Hit
TAGCAAGGGGGATCCCCAGGATCTCCAGGCTCTCCTGGATCCGTACCTAAAA1620.11606164162744213No Hit
AGCCTGGGTAACAGAGTGAGACTCCGTCTCAAATGAATGAATGAATAAATAA1620.11606164162744213No Hit
GGCAGAGGGGCCATACTACTGTTGTGAATTTACTCAACAGATTATTTTCAAA1620.11606164162744213No Hit
ACCCTGGGGTGAGCATTTCTTGTAGGGCAGGTCTAGTGGTAATGAACTTTCC1610.11534521174085298No Hit
TAGAGCGGGGATCTTAACACGGGCATAGCCAAGGAGGTGGGACTCCCTGGAG1610.11534521174085298No Hit
CGATAGGGGCAAGCAATTCTTCTGCCTCTGTCTCCCAAGTAGCTGGGATTAT1610.11534521174085298No Hit
ACACTTGGGCATTCCTAGCTTGGGAAGCTTCCGCTTAGAGGTCCTGGCGCCT1600.11462878185426384No Hit
AGGCTTGGGGATGAAAACCATCGCCCCTTGAGTGCTGCATCCAACAGTGATC1600.11462878185426384No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCCTGTCTCTTATAC1600.11462878185426384No Hit
AACAATGGGGCAGCCTTAAGAGCCGTAACACTCACTGTGAAGGTCTGCAGCT1600.11462878185426384No Hit
AAGCGGGTGTCTCAGGAGGAGGTCTGCGATTTGCTCCATGCCGCACCCTTCC1590.11391235196767469No Hit
AATGCTGGGCAGATGAACATATGGCAGAGACTAGTTACAAGACTCCTACTGG1580.11319592208108553No Hit
TTTAGGGGGATTACATTAAATCTCTAGATTACATTCAGCAGCATGGACATCT1580.11319592208108553No Hit
CTCAATGGGTGTTCTCTGTGGTCTGCCTGGGCTGGGGGTTAGGCAAGGCAAG1580.11319592208108553No Hit
AGCAGAGGGTATTTATATTTTTAGTAGAGACAGGGTTTCACCATATTGGCCA1580.11319592208108553No Hit
GCCCATCTCGGCCTCCCAAAATGCTGAGATTGCAGGCGTGGGCCACCATTCC1580.11319592208108553No Hit
GAACATGGGTAGCCGGGCAAGCAGGAGCAGGTCTCTCTGCATCTGTTCTCTG1570.11247949219449639No Hit
GTCATAGGGAGCTTGAAGGGCTGTTGAATTTTGTCAAAAGCCTTTTCTGCAT1570.11247949219449639No Hit
GTATTAGGGAAGATCTGTAATACTTGGAGATAGGAACTATGTCACATAGTGC1560.11176306230790724No Hit
TTGCGAGGGGGGGTTGTGAAAGGTGTGATGGATGTCAGATGTTTCCTATCAA1560.11176306230790724No Hit
GCCACGGGGAGCCCAGCCGGGCTTCAGTTCCATATTTGCCATGTGTCTGTCC1560.11176306230790724No Hit
CTGACATCGGGGTGGGTGATTATGTATCCCTTTTGCTTTTCAGTGTCTTCTC1560.11176306230790724No Hit
GTATCGGGGGTAGCTACCTTGTGAGGACTAAGTCAGGCAGCCCCTAGGAGCT1560.11176306230790724No Hit
CGTAAAGGGAATAATTTAATGACTTTAGATGATATGCTAAAGAAATGATGGT1550.11104663242131808No Hit
CAATAAGGGCATTAAAGTGGAATGAAATATGAAGATGCAGTAAAGTTCGTAA1550.11104663242131808No Hit
TCAGGCGGGTATACTGTGTGTTTAAAACATGTTTTAATTTTAGCAGCATCAT1550.11104663242131808No Hit
GCTTGTGGGATATGCTCTCCCAATGAACAAGACATGCAGTGAAGAAAGAAAC1550.11104663242131808No Hit
GCTACAGGGGCGCCGAGACCTCAAGGTGTAGTCCTCTTTCCCGAGGGGACTC1540.11033020253472894No Hit
AGATATGGGCTCTGACAGACACGGTAACTAAATGTGAAAAACCAATAAGCTG1540.11033020253472894No Hit
ATCGCTGGGGTATGTTATCATGACCTTTGCAGGGGTTTAGAATTATGGGGTC1530.10961377264813979No Hit
ATTTGCGGGGAGATGATGTGCTAGGAAGAATGTAGTCTCTTGGAAAAACAAA1530.10961377264813979No Hit
AATCTAGGGGATGGGAGTTTGGTGGGTATTACCTCCAGTTAAGAGCCATTGA1520.10889734276155064No Hit
GGCACTGGGATTAAATATAAGTGGACAAAGACTCCAGTTAAAAGATTAAAAT1520.10889734276155064No Hit
CGTTACGGGTGAAGAAGGTGGAGGAGAAGAAGACCAGGACTATGACTTGAGC1520.10889734276155064No Hit
ATAAATGGGGATTCTCAGAGCTACATATTATTTTACCGAAAAGCCATAACTA1520.10889734276155064No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTA1520.10889734276155064No Hit
GTGAATGGGAAGTATTCACCAGTTAATAGCACCAGGTGGGATAAAACTTTTG1510.1081809128749615No Hit
TCAATAGGGGTCACTGTCACTGTCCTCTTTGCCTTAGTGCTTTCAGGAGCCC1510.1081809128749615No Hit
ATGCTGGGGTGAGCTGCGTTTGGCAATTGAGGTGGTGTTTAAAGGTGAACTT1510.1081809128749615No Hit
TTGTAAGGGAGAGATTCTAAGCCCATATAAGGAGATGAAAAATAAGCTTACA1500.10746448298837234No Hit
CCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1500.10746448298837234No Hit
AATCTAGGGTCAATTCCAAGTTAAGCAAGATTATATTGAATTCAGCTTTACT1500.10746448298837234No Hit
GCGTATGGGGTCTTGGTTTGCAGTGGGATGTTACTATCTCATGGTCGGTCAT1490.10674805310178319No Hit
TTCTCTGGGGAGTGTCACAGGGCAGGCCTGAAGAACTCTGCTTTCAGCTTCG1490.10674805310178319No Hit
CCGTACGGGGGAAGAAAGGAAAGAAAGAAAGAAAGACAGACAAAGAAAGAAA1490.10674805310178319No Hit
AGCTTAGGGAACCACACATTATAAGGGGCCAAGAATAGTGCTTGCTGCTGCT1490.10674805310178319No Hit
TAATAAGGGCTAAACATAAATTGTAAAGATTTCATGGACACTTATCACTTCC1490.10674805310178319No Hit
TCAAGTGGGAATCATTGTTGAGGAATTACAGAGACAGTGAGATATGTCAGAA1480.10603162321519405No Hit
AGCAAAGGGAGATAGGAGAAGAGAGAGATCGCTGTCTCGGGAGAGAAATCAC1480.10603162321519405No Hit
CCAGTAGGGAGGTTTCAGAGCGCAGCCATCGGTGCGCTGCAGGAGGCTAGCG1470.1053151933286049No Hit
TTGCAAGGGTACTATGTCTATGTAGGGAAAAAAACAAAACAAAAAATTGCAC1470.1053151933286049No Hit
ATAGTGGGGATGGAGAAACCTGTAATCGCTTATTAGAGACCGAAGTATTTGT1460.10459876344201574No Hit
ACTGCCGGGAATTGAGTGCAATGTGGTATTAACTTAATTGAAAGTGATATGT1460.10459876344201574No Hit
CGGCACGGGGTGATGGCGCTCGCCCGTGGTCCCGGCTACTCTGGAGGCTGAG1450.10388233355542659No Hit
CGCTACGGGACATTTCCTGGGATATGTTTTTGGAGGTTGCTCAGATCACGGC1450.10388233355542659No Hit
ATGACTGGGTACAGTTCCCTGCTCCACTCTTTCATTCTCTTAACAAATAATT1450.10388233355542659No Hit
CTACAGCGGGATCTCTGAACATTTGCTTTTGGCATCTTCTTCAGTAGCCCAA1450.10388233355542659No Hit
TGTGCTGGGAGTCCCAGCTACTTGGGATGCTGAGGCAGGGGGATCACATGAG1440.10316590366883745No Hit
CACTATGGGAGGAGAGGGTGGAGGGAGTTTGATTTTAGAAAAAGAGAAAGGG1440.10316590366883745No Hit
CACCCAGCAGATGTTTGATGCCAAGAACATGATGGCTGCCTGCGACCCCCGC1430.1024494737822483No Hit
CTATGCGGGCGATCTGTTGCTGGGGCCTTTTCCTCAAGGGAGCAACGTTAGC1430.1024494737822483No Hit
CTAGCCGGGAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTTCTCGGC1430.1024494737822483No Hit
GATAAGGGGCCAGGCAGTGAGGAATCCTGAAACACCAGGAGAGGCGTCAGAA1430.1024494737822483No Hit
TAACGAGGGCTTACATTTTACAGTAGTTTTACCTGGAAAAAGTGGTACCTAC1430.1024494737822483No Hit
ATTCAGGGGTACTCTATAGCCTTGGTACCTTGAAAGTGTTTAAAGCACTCAG1430.1024494737822483No Hit
TGCCGCAGGGATTCTTTAACTCCAATCAATGGTGATAAATATATTTTATATC1420.10173304389565914No Hit
AGTAGTGGGCCTTTGATATGGTTTGGCTATGACCCCACCCAAATCTCATCTT1420.10173304389565914No Hit
ATACAAGGGCACTAATCTTTATTTCTGTTATTTCCTATTTGACATTTATCTG1410.10101661400907No Hit
TATCACGGGGCACAATCACAGCTCGCTGCAGCCTTGACCTCCCGGGCTTAGG1410.10101661400907No Hit
CTGGCTGGGGGACGCTGCGGGGCGGGCGGACAGACTGCGGGACGGACGGTGG1410.10101661400907No Hit
GATACAGGGACTGATTACTTGGTAAGGCAATTTTTTGTTCCAGGACCATTCT1400.10030018412248085No Hit
TTCAAAGGGGATCCCTGAAAACTAATGTTATTTCCTAAAATTACTGTCCCTC1400.10030018412248085No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)