FastQCFastQC Report
Sat 31 Dec 2022
SRR5845154.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845154.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138270
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT15911.1506472842988356No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC14871.0754321255514574No Hit
AAGAAGGGATTACACCAACATTTATTTAAACAGAATCTATAAAAATCATATG2760.19960945975265784No Hit
GTAAGCGGGGTTTGGCTCTGTGTCCCCACTAAAATCTCATCTCAAATTGTAA2660.19237723295002532No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATT2450.177189556664497No Hit
ACCAAGGGGCTGGTAGGGCCTGCAAACCTGCCCTACCAAACCTGAAAGAGGG2310.16706443914081145No Hit
TATATTGGGCAGTCCCAGAATTGGCTTAGCACAGAGACTCTAAATGATAGTA2260.1634483257394952No Hit
GCTTCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2250.16272510305923193No Hit
CTTGCAGGGGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTT2200.15910898965791567No Hit
AGTTTTGGGGCTTGATGGGGATGGCATTGAATCTGTAAATTACCTTGGGCAG2160.15621609893686267No Hit
AATAGTGGGCAGCCTGGTTTTAATGATGTTGATATGTTTGACAGGTCTCATT2060.14898387213423012No Hit
CAGCCTGGGTGACAGAGTAAGACCCTGTCTTAAACAAACAAAAAATTAAAAA2060.14898387213423012No Hit
ACTTATGGGAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGA1990.14392131337238734No Hit
CTGGTTGGGTACATATTTCATTCATTTTGCCAGCATACCATATAGACAAGTT1960.14175164533159762No Hit
AAGTACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1940.1403051999710711No Hit
TCCGTGGGGGGTGTGACTTTGTTCCAGTAAACTTTCTTTATAGAATGGGAGA1930.13958197729080785No Hit
TTAACGGGGTAGGCAAGGGACTCTTGGTCTCCTACTTATCCCAGGGAGCCCT1870.13524264120922833No Hit
TACGCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1860.13451941852896507No Hit
AACAATGGGGCAGCCTTAAGAGCCGTAACACTCACTGTGAAGGTCTGCAGCT1850.1337961958487018No Hit
CTCTCAGGGACCCACAAATCAGGAGTGAGATGAATGTCGTGATTATTTTTTG1840.13307297316843858No Hit
ACATGCGGGATTGTCAACTATGATTACAAAGTAGTCCATTCTCAAGGTAAAC1840.13307297316843858No Hit
ACAAAAGGGCCACCTCACCCTCTCCGCACACCTCTGGCTGTCTTTTTGTACT1840.13307297316843858No Hit
GATTCAGGGATATGTCTGTAGAATTTTCATCTGTGTTAGCTGAATGAGCTAC1830.1323497504881753No Hit
ATTTCAGGGCTGGGAAGAACTTAGCAAGGCCTGTTTGCTCAGATTTTTCTCT1830.1323497504881753No Hit
ACGCTGGGGGATAGTATCTCACATCTCAATATTAATGTTGAATGTAAATGAC1810.1309033051276488No Hit
GTACTAGGGGTGGCCACAGTCAGTTTCTTCTGGGAGGAGAGCTTGACCCAGC1800.13018008244738555No Hit
ATGTAGGGGAGGGAAGTGTCTTACCTATTGCTTTTTTGCAGAGGATATTTTA1800.13018008244738555No Hit
CCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1800.13018008244738555No Hit
GCCGCAGGGGAATTCGAGGAAGATGATGTGAATCTTCCAATTTTATCTATTT1790.1294568597671223No Hit
TCACGTGGGGGAAATGTACATCTCTAGCCATCAACTAGTAACAAGAATGGCG1770.1280104144065958No Hit
TGTTTGGGAACTGTAAAATAAATACATTTGAAAAAGGCACCCTCTCAACCTT1770.1280104144065958No Hit
TTTGTAGGGGCCTGAATTTGATACGCATGCAATGAGTACTTTTACAAGAAAA1770.1280104144065958No Hit
TTTAGGGGGATTACATTAAATCTCTAGATTACATTCAGCAGCATGGACATCT1760.12728719172633254No Hit
TATGCAGGGACCACCAAAGTTACTATGAAGCCTGCTGGGTTTGGAAACCTAG1760.12728719172633254No Hit
AATGCTGGGATAATAGAATAGCCAAATGCATACTCTTCTTCTCACTAGAAGG1760.12728719172633254No Hit
TGTTCTGGGACCTCGTGATCTGCCCACCTTGGCTTCCCAAAGTGCTGGGATT1750.12656396904606929No Hit
ATCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1750.12656396904606929No Hit
TAGCAAGGGGGATCCCCAGGATCTCCAGGCTCTCCTGGATCCGTACCTAAAA1750.12656396904606929No Hit
TAAGCTGGGAGTATCAATTATTTCTATATTTACCAAAACACCTACTACTTTA1750.12656396904606929No Hit
AGCAAAGGGAGATAGGAGAAGAGAGAGATCGCTGTCTCGGGAGAGAAATCAC1740.12584074636580603No Hit
GACAGCGGGGACTGCTGTAAGGACTGAATGGGCACAGAGGCTGTGACTGCTA1720.12439430100527954No Hit
TTTTGCGGGCATTTATAATTCCAGTATTTGTTGTTATCTAGAAAATGGCTGA1710.12367107832501627No Hit
TGCTGGGGGAGGGAAAATAATACAGAGTTTAACATTTTTAGATTGAAAGACA1690.12222463296448977No Hit
GATACAGGGATTTGAGGACATGGTGTTTTGTAACCTGTTGTTTTTTCTTAAT1680.12150141028422652No Hit
GTGGCTGGGTAATTATTACTAGACCTAAGAAATGAGATAGATGGCAACACAA1680.12150141028422652No Hit
GAGCAAGGGCAGCTCACCACAACTTCCGTCTCCCAGGTTCAAGTGATTCTCC1670.12077818760396325No Hit
AGCTTAGGGAACCACACATTATAAGGGGCCAAGAATAGTGCTTGCTGCTGCT1670.12077818760396325No Hit
CACTGTGGGAACACCACTCATGCAACATTCCAGCCAGGCATGGATAACCCAA1670.12077818760396325No Hit
ATTCGAGGGGACATGTAAAAATGGTGGTTCTCTACTCCAGGTTTTTATTTCT1660.1200549649237No Hit
GTCATAGGGAGCTTGAAGGGCTGTTGAATTTTGTCAAAAGCCTTTTCTGCAT1660.1200549649237No Hit
AATGCTGGGCAGATGAACATATGGCAGAGACTAGTTACAAGACTCCTACTGG1640.11860851956317349No Hit
AGGCTTGGGGATGAAAACCATCGCCCCTTGAGTGCTGCATCCAACAGTGATC1640.11860851956317349No Hit
CTCTTGGGGGAGGAAAATTATAAGGTAAAATTTTCCTGTTTTTCCCCCTTTG1640.11860851956317349No Hit
AGATATGGGCTCTGACAGACACGGTAACTAAATGTGAAAAACCAATAAGCTG1620.117162074202647No Hit
ACTAGAGGGTACCACTGCCTCATACTTTCTTCTACATCAGAGAATTCATAAA1610.11643885152238374No Hit
ATAAATGGGGATTCTCAGAGCTACATATTATTTTACCGAAAAGCCATAACTA1610.11643885152238374No Hit
CAGCCTTCCCAAAGTGCTGGCATTGTAGGTGTGAGCTACTGCACCTGGCCAA1610.11643885152238374No Hit
GGTGTCGGGCAAATTTCTACATTGGGCCAGGAGAGCCTATATGTTACTATTG1610.11643885152238374No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTA1600.11571562884212047No Hit
ATGCTAGGGTCCCTCAGCTTAAAAAAATGGGACTCCTAGGGCTATTTGCCTT1580.11426918348159398No Hit
GTATATGGGACACTACGCTCTTCCGATCTAGTCTGGGCAGTGAAAATGTGGT1580.11426918348159398No Hit
TTCATAGGGGGGATCGTGACAAAACATACAGGAGCTGATAACCAAAATAGCC1570.11354596080133074No Hit
GAACTAGGGTGAAGGTAGAGGTGACATAAAAGTAGGCTGTATATTCAAATCC1570.11354596080133074No Hit
GGCACTGGGATTAAATATAAGTGGACAAAGACTCCAGTTAAAAGATTAAAAT1560.11282273812106747No Hit
TTCTATGGGGGGGCCCCTGATGCCAAGAGCTCTGGAGGTTCTTGAAAGAGGG1560.11282273812106747No Hit
AATCTAGGGGATGGGAGTTTGGTGGGTATTACCTCCAGTTAAGAGCCATTGA1550.11209951544080422No Hit
GGCGATGGGCCTGGAACTTGAGTTATTAAAATCATAGGAATAAAATTATGGG1550.11209951544080422No Hit
TAGCCTGGGGTTGCAGTGAGCCGAGATGGTGCCATTGGCTGTGCCTGGGTGA1550.11209951544080422No Hit
GATCCGGGGTAGCCAAGGTTAAGAACCAGTACCGTACGTACAAAAAAGTTGA1550.11209951544080422No Hit
AGCCGGGGGAAGTAAAAAAGATTTTTTAAAATTTTGATGAAGCCCAATTATG1550.11209951544080422No Hit
CAATAAGGGCATTAAAGTGGAATGAAATATGAAGATGCAGTAAAGTTCGTAA1550.11209951544080422No Hit
TCAAGTGGGAATCATTGTTGAGGAATTACAGAGACAGTGAGATATGTCAGAA1550.11209951544080422No Hit
CATCCAGGGGATCCCGCGGTCCCAGGACCCCCGGCAGCAGCTGCCACGCTCA1550.11209951544080422No Hit
CACTATGGGAGGAGAGGGTGGAGGGAGTTTGATTTTAGAAAAAGAGAAAGGG1550.11209951544080422No Hit
CTTGTGGGGGGCATCAGCTAAATCTCTGTCATATTGTTTATATTTTTTGCAA1540.11137629276054097No Hit
CTTACGGGTTAGGGTGGAATGAAAAAACCAAACAACAAGCAAGGGGATTTAT1540.11137629276054097No Hit
GGCAGAGGGGCCATACTACTGTTGTGAATTTACTCAACAGATTATTTTCAAA1540.11137629276054097No Hit
CGTGACGGGTACTTGGGTATATATAATCAGGACCATTAAAAAGAACACGATG1540.11137629276054097No Hit
TGTCAAGGGGTCTCATCTCCCTTGTATTTTTAAATTTTGTTTTATAGATCAT1530.11065307008027772No Hit
ACACTCGGGCTGGGCTCAGGCTCTCCTCCTGCCTTAGCCCTCCAAGTAGCTG1530.11065307008027772No Hit
GATAAGGGGCCAGGCAGTGAGGAATCCTGAAACACCAGGAGAGGCGTCAGAA1530.11065307008027772No Hit
ATTCAGGGGTACTCTATAGCCTTGGTACCTTGAAAGTGTTTAAAGCACTCAG1530.11065307008027772No Hit
TATCACGGGGCACAATCACAGCTCGCTGCAGCCTTGACCTCCCGGGCTTAGG1520.10992984740001445No Hit
GTCTCTGGGTCTAACGGCGGGTGATTGCTTTAAATTAAGCACAAGAGGCCAT1520.10992984740001445No Hit
AAGCGGGTGTCTCAGGAGGAGGTCTGCGATTTGCTCCATGCCGCACCCTTCC1520.10992984740001445No Hit
TAGCGTGGGATCTGCTACTGCCCTTTCTATAATAACTAAAGTTAGCTGCCCT1520.10992984740001445No Hit
GCCACCGGGTGCGAAACTCGGCGGCCACGGCCGAGAAGTGGCGCTTCCGCCT1510.1092066247197512No Hit
GGTGCAGGGATCATATATCATATATGAATGTACATTAGTCAAATGGTGTGAG1510.1092066247197512No Hit
CTCTCTGGGATCCTTCTTCATTTGCAGCTTCTTCTTGTTTATGCTTTGGGAA1510.1092066247197512No Hit
TGCCTTGGGGGAAACTTTCACAATGTCCGGAGCCCTTGATGTCCTGCAAATG1500.10848340203948796No Hit
ATAGTGGGGATGGAGAAACCTGTAATCGCTTATTAGAGACCGAAGTATTTGT1500.10848340203948796No Hit
TCTGAAGGGATTACAGCATTAAATCATTTGGGGGTGGGGAAGCAGTGGAGTC1500.10848340203948796No Hit
TTGCAAGGGTACTATGTCTATGTAGGGAAAAAAACAAAACAAAAAATTGCAC1500.10848340203948796No Hit
CGATAGGGGCAAGCAATTCTTCTGCCTCTGTCTCCCAAGTAGCTGGGATTAT1490.10776017935922472No Hit
GCGTATGGGGTCTTGGTTTGCAGTGGGATGTTACTATCTCATGGTCGGTCAT1480.10703695667896145No Hit
ACCGAGGGGATTATCATTTGTCCCAAAAAGTACGAGGGATGTGCTGACGAGA1480.10703695667896145No Hit
ATAAGCGGGCTGTAAGTATCTACTGAGTCTCTCCATGTGCCAGATATTAGAT1470.1063137339986982No Hit
TCACGTGGGAAGGAAATGGGAAGGCTGTGTCTCTGACCTCACAAATAAATAT1470.1063137339986982No Hit
CGCTAGGGGGAGAAGAATTAGAATCCTACAAGGGACACTTTGGTCCTATTCA1470.1063137339986982No Hit
GCATCTGGGATTGTCTTAAGTATTATCTACTATGTATCTTTAACACTTTTGA1470.1063137339986982No Hit
TTGTTTGGGCAAAGCAAAAACCTATAGTAGATACACAAATGATTAAAAAAAA1470.1063137339986982No Hit
AGCCTGGGTAACAGAGTGAGACTCCGTCTCAAATGAATGAATGAATAAATAA1460.10559051131843494No Hit
GCTTGTGGGCGATCTTGCAAGGGGATCTAAAGAAGGGCTTGGTGGAAGGAAG1460.10559051131843494No Hit
TGCGGAGGGGGAGGGTAATCCTGCTCTTAATCGTGTGAGATGGACCCATTCT1460.10559051131843494No Hit
CATAAAGGGCAGGCACTTACTTTATGTCAAAAGAAAAATTAGGCATATCCAG1450.1048672886381717No Hit
CTCAATGGGTGTTCTCTGTGGTCTGCCTGGGCTGGGGGTTAGGCAAGGCAAG1450.1048672886381717No Hit
TAGAGCGGGGATCTTAACACGGGCATAGCCAAGGAGGTGGGACTCCCTGGAG1450.1048672886381717No Hit
TACATCGGGCACCAACTTTAGTATTCTAAAGCACAGACTTTGTCATGTTATC1450.1048672886381717No Hit
GCTTGTGGGATATGCTCTCCCAATGAACAAGACATGCAGTGAAGAAAGAAAC1450.1048672886381717No Hit
AGGCTGGGTGACTTGAATCCAGGAAGCCCATTTTCACACTAGCCCTCAGGCC1450.1048672886381717No Hit
GTGAATGGGAAGTATTCACCAGTTAATAGCACCAGGTGGGATAAAACTTTTG1440.10414406595790843No Hit
TTAAAAGGGCTCCTCACTCACCTCCAAGCTGATCCGTTCGCACCCAACCACT1440.10414406595790843No Hit
GCCCATCTCGGCCTCCCAAAATGCTGAGATTGCAGGCGTGGGCCACCATTCC1440.10414406595790843No Hit
TAACGAGGGCTTACATTTTACAGTAGTTTTACCTGGAAAAAGTGGTACCTAC1440.10414406595790843No Hit
CTGACATCGGGGTGGGTGATTATGTATCCCTTTTGCTTTTCAGTGTCTTCTC1430.10342084327764518No Hit
CAATGAGGGACCGTCATTATTTCGTATGCCTGTTTCATTGCAAGACCTTAGA1420.10269762059738194No Hit
CGCTACGGGACATTTCCTGGGATATGTTTTTGGAGGTTGCTCAGATCACGGC1410.10197439791711868No Hit
GCTAGGGGGCAATCTAAATTAGTACTTTTATACATTCTATTTGGGGAGTACT1410.10197439791711868No Hit
TCAGGCGGGTATACTGTGTGTTTAAAACATGTTTTAATTTTAGCAGCATCAT1410.10197439791711868No Hit
GAACATGGGTAGCCGGGCAAGCAGGAGCAGGTCTCTCTGCATCTGTTCTCTG1400.10125117523685542No Hit
GTAAAAGGGTAGGTCTGCATTACTGATTTGTAAGCCCCTAAAACAGTTTGCT1400.10125117523685542No Hit
TCGAAAGGGGTCACTCAGGAAAAGCACCTTTCTACACCTGCCTGCATTTAAC1400.10125117523685542No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCCTGTCTCTTATAC1400.10125117523685542No Hit
ATTTGCGGGGAGATGATGTGCTAGGAAGAATGTAGTCTCTTGGAAAAACAAA1400.10125117523685542No Hit
AATCTAGGGTTCTTCATGGTGGTCAGGCGTGTCTCTAACTCCCAACCTCAGG1400.10125117523685542No Hit
TGTGCTGGGAGTCCCAGCTACTTGGGATGCTGAGGCAGGGGGATCACATGAG1390.10052795255659217No Hit
TTTCTTGGGCTGAACCTAAACTTCAGTGCTATGCAGTTTTATGTGTCATCCT1390.10052795255659217No Hit
GAATATGGGCAACCTGGGCACCGGGCTGCGGGCAGGTGTGCATATCAAGCTG1390.10052795255659217No Hit
AAGCTTGGGCAAGCTGTGTGCTGGGAGAACCACTACTCTCTTCAAAGCTGTC1390.10052795255659217No Hit
AGTCAAGGGTGTGGCTTGAATGCCTTCCCCTCATCTTGCCATGCTTGGCTGT1390.10052795255659217No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)