FastQCFastQC Report
Sat 31 Dec 2022
SRR5845147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217794
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT28001.2856185202530832No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC23761.0909391443290448No Hit
TGAACGGGGGTCTCGCTATGTTGCCTAGGCTGGTCTCAAACTCCTGGATTCA3750.17218105181960935No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATT3690.1694261549904956No Hit
TGTTAGGGGCAGAGCAGAAGCAGCTGCTGGAGGTGGAACTCTACGCAGACTA3400.15611082031644583No Hit
CATTCTGGGGCCTCCACTTTTAGTGATTCTATAAGTTATCCTCTAAAAAACA3070.14095888775632018No Hit
TATTGTGGGGTGTGTGACGCGGATATGTTCTTTCTTTTCAGGTTATCACCAA3070.14095888775632018No Hit
TGCAGGGGGGCTGCAGCACGAGACGCAGGAGCTGGCCTCCAAGCGGGTGGAC3070.14095888775632018No Hit
TAGCAAGGGAATCCACATCTCTCCAGAGCTCTTTGTTCTCATGGGTGGCACC3040.1395814393417633No Hit
TGGTTTGGGACAGCTTAAAAAATAAAAATAAAAATAAAAATAAAAACCAGCC2950.1354490940980927No Hit
TCGTCAGGGGTGGAGCAGGGTTCTGGGTGACCATGACAATGATTTACTTACT2910.13361249621201687No Hit
TTGCTGGGATGGTCACCTTGCATGAGGATGTCGTAAAGTCAGATGGGGCAAC2670.12259290889556186No Hit
CACCATGGGGAGAAACAAAAGATAAAGAAAAAATGTTCAGCTCTATAAAAGA2660.1221337594240429No Hit
TGTAAAGGGGGACGCGGTGACTCGCAGAGCACTGACGCACTCTGCGCCCGGA2660.1221337594240429No Hit
ATTAGAGGGGGATCAGGAGACAGTGAAGATGAGAGGAGTGACAGAGGATCTG2620.12029716153796707No Hit
GCATTGGGGACCAGGGAAGACACTTAGGAGACACTTTTGGCGAAGACTGGCA2580.11846056365189123No Hit
AACTAGGGGCAATCCAGGGACTTGGGTATACTGCAAGCTGAGATTTTATCTT2580.11846056365189123No Hit
AACTCAGGAGAGGTAAGCAGTGGCAGAACACTCTGAGAGCTATGGCTTTAAC2560.11754226470885332No Hit
TGGCATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2430.11157332157910686No Hit
TTTCCAGGGTGGGGCTTTCCCTAAGAAGAATCCATCCCAGCACATATTTTGA2410.11065502263606895No Hit
TCTCCAGGGGAGAATGTGTGGAAAGAAAAGTGGCTCCAGTGGCAAAAGTAAA2400.11019587316455No Hit
GGCCCAGGGCACCCTATGAAAATGGAGTACAAGGCATGCTGAATACTTCAAA2370.10881842474999312No Hit
TGATATGGGAGATGTTCAAAAGGAAGTGTCCAGTGAGCAGCTGGATGTGGGG2360.10835927527847417No Hit
CGCACAGGGTACTGTTTGTTTAAAGCATGCATAAAAATTTATGTATTGAAAT2340.10744097633543623No Hit
TCGCCGGGCTAAAAAACTTCTGTAGGACCAACGAAACAATTAACAGAATGAA2340.10744097633543623No Hit
TGCCTGGGGGGAGAAGTCACCTCAAGTTTGGTGACAGATCTTCTCATGTGAT2340.10744097633543623No Hit
CATTATGGGGGAGGACAGAACATTGTTGGGAGGAGTCCAGGCATAAAGTGAC2300.1056043784493604No Hit
TTAATAGGGAGGGAAGGAAGAAAGGAAGGCAGGACTGGGAAAAGAAGGGGGC2290.10514522897784144No Hit
GAGGCAGGAGAATGGCGTGAACCCGGGAGGCAGAGGTTGCAGTGAGCCGAGA2290.10514522897784144No Hit
TTCAGGGGTGAGGAATGTGAGGCACATTATTGGGGAAATTGAGGAGACAGCC2280.10468607950632249No Hit
GTATCTGGGATCGTCTTCATTTTGTGTGTACAGTGCTGTGTGTAAGCTTATC2270.10422693003480353No Hit
TCTGTTGGGCGTGTGTTCATCATGGACAGCTGTGATGAGTTGATACCAGAGT2260.10376778056328456No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTA2180.10009458479113291No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)