FastQCFastQC Report
Sat 31 Dec 2022
SRR5845146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219740
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT26541.2077910257577138No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC24191.100846454901247No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATT3720.16929098024938563No Hit
TCGTCAGGGGTGGAGCAGGGTTCTGGGTGACCATGACAATGATTTACTTACT3710.16883589696914536No Hit
TAGCAAGGGAATCCACATCTCTCCAGAGCTCTTTGTTCTCATGGGTGGCACC3550.1615545644853008No Hit
TGTTAGGGGCAGAGCAGAAGCAGCTGCTGGAGGTGGAACTCTACGCAGACTA3550.1615545644853008No Hit
TGAACGGGGGTCTCGCTATGTTGCCTAGGCTGGTCTCAAACTCCTGGATTCA3280.14926731591881315No Hit
CACCATGGGGAGAAACAAAAGATAAAGAAAAAATGTTCAGCTCTATAAAAGA3120.1419859834349686No Hit
TATTGTGGGGTGTGTGACGCGGATATGTTCTTTCTTTTCAGGTTATCACCAA3120.1419859834349686No Hit
TGGTTTGGGACAGCTTAAAAAATAAAAATAAAAATAAAAATAAAAACCAGCC3030.13789023391280605No Hit
CATTCTGGGGCCTCCACTTTTAGTGATTCTATAAGTTATCCTCTAAAAAACA2980.13561481751160462No Hit
TGCAGGGGGGCTGCAGCACGAGACGCAGGAGCTGGCCTCCAAGCGGGTGGAC2940.1337944843906435No Hit
TTCAGGGGTGAGGAATGTGAGGCACATTATTGGGGAAATTGAGGAGACAGCC2920.1328843178301629No Hit
TTGCTGGGATGGTCACCTTGCATGAGGATGTCGTAAAGTCAGATGGGGCAAC2890.13151906798944207No Hit
ATTAGAGGGGGATCAGGAGACAGTGAAGATGAGAGGAGTGACAGAGGATCTG2810.1278784017475198No Hit
ATTCGCGGGGGGCTCCGAAGCGGGGAAGTGGGACAAGATGGTTTACATCTCG2580.11741148630199326No Hit
TGTAAAGGGGGACGCGGTGACTCGCAGAGCACTGACGCACTCTGCGCCCGGA2560.11650131974151269No Hit
TCTCCAGGGGAGAATGTGTGGAAAGAAAAGTGGCTCCAGTGGCAAAAGTAAA2530.11513606990079185No Hit
TGCCTGGGGGGAGAAGTCACCTCAAGTTTGGTGACAGATCTTCTCATGTGAT2480.11286065349959043No Hit
TGGCATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2460.11195048693910985No Hit
AACGATGGGACATCAGAGAAATAGAGGATTTGGCAAAAGAACACAACAAACC2420.11013015381814872No Hit
TTTCCAGGGTGGGGCTTTCCCTAAGAAGAATCCATCCCAGCACATATTTTGA2410.10967507053790844No Hit
AAAGGTGGGGTACAAACATCGCTTGAAGTCTTCACATCTACCACTAGACACC2390.10876490397742787No Hit
TGATATGGGAGATGTTCAAAAGGAAGTGTCCAGTGAGCAGCTGGATGTGGGG2390.10876490397742787No Hit
TCGCCCAGGGGCCCGGCCACATTTATTTCTTTTTGAGACAGCCTCGCTCTGT2330.10603440429598618No Hit
GCACCTGGGTCTCCCAAGAGGGGTCCCCCAACTCACCGTTCCCGGGACAGGC2320.1055793210157459No Hit
GAGGCAGGAGAATGGCGTGAACCCGGGAGGCAGAGGTTGCAGTGAGCCGAGA2320.1055793210157459No Hit
AGGTAAGGGCAGGAGCGAGATCCCTCCAAAATCAAGTGGGGCGATGCTGGCG2320.1055793210157459No Hit
AACTCAGGAGAGGTAAGCAGTGGCAGAACACTCTGAGAGCTATGGCTTTAAC2280.10375898789478474No Hit
GCATTGGGGACCAGGGAAGACACTTAGGAGACACTTTTGGCGAAGACTGGCA2280.10375898789478474No Hit
CACGTGGGGGTATGATACAAGGACAGAGAATAATGGGGACATGGAGGGTTTT2250.10239373805406389No Hit
TTTCATGGGCTGATCAGTCTAAGATGGGTGGGGACGTCCTGCCACCTGGGGC2250.10239373805406389No Hit
TGACGCAGGGCGGCAGCTCCATCCTGTCCATCCTGCTGGAGGAGGTCAGCCC2240.1019386547738236No Hit
GTGGACGGGACAGCAGCCTCTGTGGTCTGCCTGGGGTGGGGATCAGCCAGGG2230.10148357149358334No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)