FastQCFastQC Report
Sat 31 Dec 2022
SRR5845145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213354
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT24431.1450453237342633No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC22661.0620846105533526No Hit
TGAACGGGGGTCTCGCTATGTTGCCTAGGCTGGTCTCAAACTCCTGGATTCA3940.1846696101315185No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATT3730.17482681365242742No Hit
CATTCTGGGGCCTCCACTTTTAGTGATTCTATAAGTTATCCTCTAAAAAACA3560.16685883555030606No Hit
TAGCAAGGGAATCCACATCTCTCCAGAGCTCTTTGTTCTCATGGGTGGCACC3330.1560786298827301No Hit
TATTGTGGGGTGTGTGACGCGGATATGTTCTTTCTTTTCAGGTTATCACCAA3000.14061137827272982No Hit
TGCAGGGGGGCTGCAGCACGAGACGCAGGAGCTGGCCTCCAAGCGGGTGGAC3000.14061137827272982No Hit
TCGTCAGGGGTGGAGCAGGGTTCTGGGTGACCATGACAATGATTTACTTACT3000.14061137827272982No Hit
TGGTTTGGGACAGCTTAAAAAATAAAAATAAAAATAAAAATAAAAACCAGCC2890.13545562773606307No Hit
TGTTAGGGGCAGAGCAGAAGCAGCTGCTGGAGGTGGAACTCTACGCAGACTA2730.12795635422818413No Hit
CACCATGGGGAGAAACAAAAGATAAAGAAAAAATGTTCAGCTCTATAAAAGA2710.1270189450396993No Hit
ATTAGAGGGGGATCAGGAGACAGTGAAGATGAGAGGAGTGACAGAGGATCTG2620.12280060369151738No Hit
TTGCTGGGATGGTCACCTTGCATGAGGATGTCGTAAAGTCAGATGGGGCAAC2590.12139448990879009No Hit
TTCAGGGGTGAGGAATGTGAGGCACATTATTGGGGAAATTGAGGAGACAGCC2580.12092578531454766No Hit
TCTCCAGGGGAGAATGTGTGGAAAGAAAAGTGGCTCCAGTGGCAAAAGTAAA2410.11295780721242629No Hit
TCGAACGGGGTGGTTTTCTGCCATACTTTTCTCCATTGGCCCAGGTAGGCTA2400.11248910261818386No Hit
GAGGCAGGAGAATGGCGTGAACCCGGGAGGCAGAGGTTGCAGTGAGCCGAGA2390.11202039802394144No Hit
AAAGGTGGGGTACAAACATCGCTTGAAGTCTTCACATCTACCACTAGACACC2280.10686464748727467No Hit
AACTAGGGGCAATCCAGGGACTTGGGTATACTGCAAGCTGAGATTTTATCTT2250.10545853370454739No Hit
GTGGACGGGACAGCAGCCTCTGTGGTCTGCCTGGGGTGGGGATCAGCCAGGG2250.10545853370454739No Hit
ACGCGCGGGTGATCTTGCTGCAGCCACAGTGCAGCTCCACATTAACTCTACA2230.10452112451606249No Hit
GCATTGGGGACCAGGGAAGACACTTAGGAGACACTTTTGGCGAAGACTGGCA2210.10358371532757764No Hit
GTATCTGGGATCGTCTTCATTTTGTGTGTACAGTGCTGTGTGTAAGCTTATC2210.10358371532757764No Hit
TCGCCCAGGGGCCCGGCCACATTTATTTCTTTTTGAGACAGCCTCGCTCTGT2200.1031150107333352No Hit
TCTGTTGGGCGTGTGTTCATCATGGACAGCTGTGATGAGTTGATACCAGAGT2190.10264630613909277No Hit
AACTCAGGAGAGGTAAGCAGTGGCAGAACACTCTGAGAGCTATGGCTTTAAC2170.10170889695060792No Hit
CGAAGTGGGGAGGTCAGAGTTTCCTGAAAAATTAGGTGCAAGAAAAGTAAGG2170.10170889695060792No Hit
GCACCTGGGTCTCCCAAGAGGGGTCCCCCAACTCACCGTTCCCGGGACAGGC2160.10124019235636549No Hit
GGTAGTGGGCTCATTTTAAATATCTTTTTCTAACATCATATGTTGGAGCCCT2160.10124019235636549No Hit
TGATATGGGAGATGTTCAAAAGGAAGTGTCCAGTGAGCAGCTGGATGTGGGG2150.10077148776212305No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTA2140.10030278316788062No Hit
TGGCATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2140.10030278316788062No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)