FastQCFastQC Report
Sat 31 Dec 2022
SRR5845144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253981
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT16990.668947677188451No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC15240.6000448852473217No Hit
GCGTCAGGGAGACATGGCGCTTGCCAGCGTGTTGGAGAGACCGCTACCGGTG3240.1275685976510054No Hit
TAACATGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT2950.11615042070076108No Hit
TTGACAGGCATAATACTTTGTTATTGCTTTTGCTTTAAGTGATCAATGATTG2930.11536296022143389No Hit
AAATTCGGGGCTGAATGAGTACGTGGAGGTAGTGTGGCGTGCACAAATCTGC2880.1133943090231159No Hit
AATACAGGGATGCAAACAGTGTTGTTACCAGCTTAAAAATGAGTATAAGGTA2800.11024446710580713No Hit
ATGCATGGTGGTTTCTCATTGGTGATGAAGTGGAGCTTTCAAAATGAATCCA2710.10670089494883475No Hit
ACAAAAGGGACCTGGGAGGCGGAGGTGGCAGTGAGCTGAGATCGTGCCATTG2710.10670089494883475No Hit
TGAATCGGGAGAGCAGAGGAATGAGTGAATGTGCCCTGGAATCTGATGCAGA2690.10591343446950756No Hit
GCCCATGGGGGAGGCCGGTCCTGCAGGCGGCAGCCAGAGCTGCGCGCCGCGG2680.10551970422984396No Hit
TTGTATGGGAGGGCTGCAGAAGGAGGCTGTGCAGAAAGAGGAGCCCATCCCA2660.10473224375051678No Hit
TAGTTGCGGGGAGATGCTTTAAAAATATTCCCTTTTGTATAGTGAAACAACT2650.10433851351085317No Hit
AGCGTTGGGGGAAAAAGACACAATAAAAAACCAAAACCTGGAAAAGAAATAC2630.10355105303152597No Hit
AACAATGGGGATGCTTATCTAATAAAATATCACTGAGCAATAAGGAGAAATA2610.10276359255219879No Hit
CACCTTGGGGCTGTATTTTTGAAATGCTAATAATTAAGAGTTAACTGTGTTT2600.10236986231253518No Hit
TAACTTGGGATGGGAGAAGATACTGTGGCAACCAGGAAGGAATGGAAAAAAA2580.101582401833208No Hit
TCAGTGGGGGGCTTTCCGCTCGGCTGTTTTCCTGCGCAGGAGCCGCAGGGCC2540.10000748087455362No Hit
GAAGCAGGGGGCAGAGCTTGAACTTTATGACAAATGATAAACTTTGCACAAA2540.10000748087455362No Hit
CGCAACGGGTCATCCAGCAGTGTTGCTCAGCTCCTACCTCTGTGCCAGGGCA2540.10000748087455362No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)