FastQCFastQC Report
Sat 31 Dec 2022
SRR5845143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254622
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT17120.6723692375364265No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC15160.5953923855754806No Hit
GCGTCAGGGAGACATGGCGCTTGCCAGCGTGTTGGAGAGACCGCTACCGGTG3220.12646197107869706No Hit
TTGACAGGCATAATACTTTGTTATTGCTTTTGCTTTAAGTGATCAATGATTG3050.11978540738820684No Hit
TTGTATGGGAGGGCTGCAGAAGGAGGCTGTGCAGAAAGAGGAGCCCATCCCA2960.1162507560226532No Hit
TTGTTAGGGTGTGCGTGGGCACTGGGAGCTGTCCTTTCAGCACCACAAAATT2960.1162507560226532No Hit
AATACAGGGATGCAAACAGTGTTGTTACCAGCTTAAAAATGAGTATAAGGTA2890.11350158273833369No Hit
TTTCCAGGGGGGAGCTGAGGCAGGAGAATCACTTGATCCCAGGATGTCGAGG2890.11350158273833369No Hit
AAATTCGGGGCTGAATGAGTACGTGGAGGTAGTGTGGCGTGCACAAATCTGC2870.11271610465709954No Hit
ACAAAAGGGACCTGGGAGGCGGAGGTGGCAGTGAGCTGAGATCGTGCCATTG2820.1107524094540142No Hit
TGTCCAGGGAAGTGAGAACATGTGGTATCTAGTTTTCTGTTCCTTCATTAGT2810.1103596704133971No Hit
CACCTTGGGGCTGTATTTTTGAAATGCTAATAATTAAGAGTTAACTGTGTTT2800.10996693137278005No Hit
TAACATGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT2720.10682501904784346No Hit
CCTCTTGGGATTACAGCTTCATTTTCTACTAGCACCTATCATAATGGTCTTA2710.1064322800072264No Hit
TAACTTGGGATGGGAGAAGATACTGTGGCAACCAGGAAGGAATGGAAAAAAA2710.1064322800072264No Hit
GTCGGCAGGGAAGAAGAAGAGGGGGAGGAGAAAGAGATAGAAGAGTAAGAGG2700.10603954096660934No Hit
AGTTATGGCCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.1048613238447581No Hit
TGGCCTGGGTGGCACAGGCCATGCCTCTACAGGACAAATGATTGGTGCTGTA2660.10446858480414103No Hit
GAATAGGGCAGTGGCCTCTGAGTATTCAAGTGAATGGAAAAGTCACATCCCT2660.10446858480414103No Hit
TGCAAGGGATGTATAACAAAGATAAAACTGGAGTTAGTTTGGTTAAGTTGGA2630.10329036768228983No Hit
TATTTAGGGTTAGACAGAAATCTACTCTTCAGGATTTTAATCTATTTAGCCT2620.10289762864167276No Hit
TAAGATGGGACCCACCTTCAAGTGATTGAGGAGAGGGTGAATCAGAGCCTGG2620.10289762864167276No Hit
CTGCTAAAGGGACCGAACGAAAAATTCTAGGCTATATACAACTACGCAAAGG2620.10289762864167276No Hit
CTAACTGGGATAGATCAAACTGTCAAAACTTCTCAGGTCACATGATGGCCCT2610.10250488960105568No Hit
TCAAACGGGAAGGGACATCTGTATTTAACTCCTTTGTAGACATGAATTTCTA2610.10250488960105568No Hit
AATTTGGGATTACTTATGCCTGCCCATTTCATATTCTTTCAATTCTGTAGGT2610.10250488960105568No Hit
GCAACTGGGCACCTCTTCATGACCACTGCCTGCCAGGAGGCTAACTACGGTG2610.10250488960105568No Hit
AACAATGGGGATGCTTATCTAATAAAATATCACTGAGCAATAAGGAGAAATA2590.10171941151982154No Hit
TAGTTGCGGGGAGATGCTTTAAAAATATTCCCTTTTGTATAGTGAAACAACT2590.10171941151982154No Hit
CTACATGGGATCCCTTCCTTTTCCTTCTGGAATCATTAAGGAGAGATTTATC2570.10093393343858739No Hit
TCAGTGGGGGGCTTTCCGCTCGGCTGTTTTCCTGCGCAGGAGCCGCAGGGCC2560.10054119439797034No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)