FastQCFastQC Report
Sat 31 Dec 2022
SRR5845142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253877
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGCCGTCT16880.6648889028939211No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCATGATTATCTCGTATGC14540.5727182848387211No Hit
ACAAAAGGGACCTGGGAGGCGGAGGTGGCAGTGAGCTGAGATCGTGCCATTG2960.11659189292452644No Hit
GCGTCAGGGAGACATGGCGCTTGCCAGCGTGTTGGAGAGACCGCTACCGGTG2920.11501632680392473No Hit
TAGTTGCGGGGAGATGCTTTAAAAATATTCCCTTTTGTATAGTGAAACAACT2880.11344076068332302No Hit
TAACATGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT2830.1114713030325709No Hit
AATACAGGGATGCAAACAGTGTTGTTACCAGCTTAAAAATGAGTATAAGGTA2790.10989573691196919No Hit
TGAATCGGGAGAGCAGAGGAATGAGTGAATGTGCCCTGGAATCTGATGCAGA2770.10910795385166833No Hit
TTGTATGGGAGGGCTGCAGAAGGAGGCTGTGCAGAAAGAGGAGCCCATCCCA2690.10595682161046491No Hit
CCGAATAGGGGCAACTGTGTTAATATCTACATGTTTCCTTCCAGAAGTAAAG2650.10438125548986321No Hit
CTACATGGGATCCCTTCCTTTTCCTTCTGGAATCATTAAGGAGAGATTTATC2650.10438125548986321No Hit
TTGTTAGGGTGTGCGTGGGCACTGGGAGCTGTCCTTTCAGCACCACAAAATT2640.10398736395971278No Hit
AGCGTTGGGGGAAAAAGACACAATAAAAAACCAAAACCTGGAAAAGAAATAC2610.1028056893692615No Hit
ATCAAAGGGCACTTTTGAATAGAACAAACAGCTTTTCCATTTTGCTCTGCTG2590.10201790630896064No Hit
ATGCATGGTGGTTTCTCATTGGTGATGAAGTGGAGCTTTCAAAATGAATCCA2580.10162401477881021No Hit
TCAAACGGGAAGGGACATCTGTATTTAACTCCTTTGTAGACATGAATTTCTA2580.10162401477881021No Hit
GAAGCAGGGGGCAGAGCTTGAACTTTATGACAAATGATAAACTTTGCACAAA2580.10162401477881021No Hit
TTTCCAGGGGGGAGCTGAGGCAGGAGAATCACTTGATCCCAGGATGTCGAGG2580.10162401477881021No Hit
AAATTCGGGGCTGAATGAGTACGTGGAGGTAGTGTGGCGTGCACAAATCTGC2580.10162401477881021No Hit
TGTCCAGGGAAGTGAGAACATGTGGTATCTAGTTTTCTGTTCCTTCATTAGT2570.10123012324865979No Hit
GCCATAGGGTATGTCAACAAAATTCAAATGGTGAAAATCAATCCTAGTGATG2550.10044234018835893No Hit
CACCTTGGGGCTGTATTTTTGAAATGCTAATAATTAAGAGTTAACTGTGTTT2540.1000484486582085No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)