FastQCFastQC Report
Sat 31 Dec 2022
SRR5845123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154192
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGCCGTCT6690.4338746497872782No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGC6130.3975562934523192No Hit
AATGCCGGGGATGTACACAGCTGTGGGCTCTGAATGGCGTCCCTTTGGCTAT2570.16667531389436546No Hit
AAACAAGGGAGACAGTTTAGTGCTATATTATTTATTCAAGTACTTTTTATCA2130.13813946248832626No Hit
AATGCTGGGGTGCATCTGATGTCCCTAATTTCAAAGTAAAATCACTGACTTA2010.13035695755940643No Hit
CAGCCTGGGGCCAAGAGCGAGAGCCTGTCTCAACAATACCAACACCACCACC1990.1290598734045865No Hit
TAAGCTGGGCTTCTGATGGGACTGCACTTACGCTATCCAGTTGCAGGTAATT1970.12776278924976653No Hit
GCACACGGGCCAGGTGCTGTTTATGTTGTAGGCACCTAAGTACTTATACAAG1940.1258171630175366No Hit
CAGCCTGGGCGACAGAGCAAGACTCTGTCTCAAAAAAACAAATTACTATTTC1920.12452007886271661No Hit
ATCAAAGGGGACAGAGCCGCGGCTTGTTTTCAGGACACGGGTCGCTGGCGGG1910.12387153678530664No Hit
TGTAACGGGAGTTGACAATCTTCATGTGAATAATCCACTGAGTGGACTGATT1880.12192591055307667No Hit
AAAACGGGGAAGCCAACATATGAATATATGTTTTGTCTCGCTATACTGCACT1810.11738611601120681No Hit
TTTAATGGGAATAAGAAACATTTTACAACCTGCTCTCTCTGAAGTCTGCTAT1810.11738611601120681No Hit
ACACATGGGGTGAACAATGGACCCCAAGTCATGTCAGTGTGGCCCCTGCTAC1800.11673757393379683No Hit
TGTTTGGGGCTGTCAACAACGTCAGTGTGGCATATTAACAAGACTAACTGCT1800.11673757393379683No Hit
TATGTTGGGGATGATTTCTAGTGACAACGACAGCATTTCTGAAGGGGTTATT1770.11479194770156688No Hit
ACTGCAGGGGCCCAACAAACTCCTGCGGATCACAGAGGATGGCACCTTGCTG1770.11479194770156688No Hit
GAACCCAGGGAGAGAGAGAGAGATAGAGGAGAAAGGAGAGAAGAGAATGAGA1750.11349486354674693No Hit
AATTTTGGGAATTATACAGATGACATGTGCTTTTTTTTCTTTTTTCAACCTC1720.11154923731451698No Hit
TACAGAGAAAGACTATGAAATACTTTTCAAATCTATTAATGGAATCCTTTTC1710.11090069523710698No Hit
GTTGTGGGGTAGCCTGGTGGGGTAGAAGGCAGAGAAGACTACCCAGCCAGTG1700.110252153159697No Hit
TTCATGGGGCAGATGTAATTCACAAGCACATGGTGACAAATTGCCAGCTTTT1670.10830652692746705No Hit
CACCATGGGCTGAACTCTCGCTTTCTTTTTAATCCCCTGCATCGGATCACCG1670.10830652692746705No Hit
ATAGCAGGGAATCCCAATTATAAAATTCCACCTAAAGGAGTTTCATTTTGTA1660.10765798485005706No Hit
TGCTATGTTACTGGTGGAACTAATTTAAACTAAGCCCTTAGCAAGATACCTA1650.1070094427726471No Hit
TTCGTAGGGGGAAGTGGTGAGAGAAGCTGGACTAGGCTTTGCTGTCAGTGTG1650.1070094427726471No Hit
TAGTGTGGGGGAGGAACAGTACCTTGGCTTGGCTCTTGACGTGGACAGAACT1640.1063609006952371No Hit
TAAAAAGGGAGATCGAAGGAAGCTATTTTTATCATATGAAAGCAAGTTTCAC1630.10571235861782712No Hit
TTAACAGGGATTGCTCAGGTAACGAGAAAATAATAAAAGATGGAGAGAACAG1620.10506381654041715No Hit
AAGGCAGGAAGAGAAGTGTCAGGGAGTCAAAGATAGAGCTAAGGAAAGGCAA1620.10506381654041715No Hit
GGTACGGGCTAATAAAATAGTGACAAGTAGAAATCTGGAGAATAATTCATAA1610.10441527446300716No Hit
ATTGTTGGGAAGAACTGTCAGGATTAGTTGGATGATAATTGACTAGTGTCTG1610.10441527446300716No Hit
ACAAATGGGGATAGTTCTGACGGAGTCTGGCAGGTCTGCTCACCAGGTGGCC1610.10441527446300716No Hit
TTTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1610.10441527446300716No Hit
CTGAGGGATAGTTGATTCCGTTGATGGAATTTTGCTTAGGAATTTTTTTAAT1600.10376673238559717No Hit
GTTGGCGGGGTGGGCCCAGTTTTCAGGCATGTAGAATAAGCATGGAGTCATA1580.10246964823077721No Hit
AGAAGAGGGAGCGCTGTATTCATTTGAAGGACAGCAGCCTGGGGATTTGAAT1580.10246964823077721No Hit
GCTAAGCGGGCCCATGTCTAACTCCATTCCAAAAGAAAAATGAGGTCAGTAG1570.10182110615336723No Hit
CAACCGGGGATGTTACACTCAGTGACAATTGTATATATGATGGTGGTCCCAA1570.10182110615336723No Hit
GCTACTGGGGGAAGACAGGGGCGATACAGCCCCTATGCCATTAATTGTAATA1570.10182110615336723No Hit
GGTCTAGGGAAAGCTAGAGCCTCGCCCCACTCAAAAGAAGTACCATAAAGAG1570.10182110615336723No Hit
CCTGAGGGGGGAGAATAGCTTGGGCCTGGGAGGTGGAGGTTGCAGTGAGCCG1570.10182110615336723No Hit
CGTACTGGGGCACTTGGCCCATAGTGAGCACATAGCACACCACCTAATAAGA1560.10117256407595726No Hit
AAGTATGGGGGGGTTCATTAAACAGCAGAGAGGGAGTGAGTTCATGATCAGA1560.10117256407595726No Hit
AGATAAGGGGAAAAATAACTATTGGGTACTAGGCTTAGTACCTGAATGATGA1550.10052402199854726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)