FastQCFastQC Report
Sat 31 Dec 2022
SRR5845122.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845122.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153733
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGCCGTCT7100.46183968308691037No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGC6080.39549088354484724No Hit
AATGCCGGGGATGTACACAGCTGTGGGCTCTGAATGGCGTCCCTTTGGCTAT2430.15806625773256228No Hit
CAGCCTGGGGCCAAGAGCGAGAGCCTGTCTCAACAATACCAACACCACCACC2110.1372509480723072No Hit
TAAGCTGGGCTTCTGATGGGACTGCACTTACGCTATCCAGTTGCAGGTAATT2090.13594999121854123No Hit
AATGCTGGGGTGCATCTGATGTCCCTAATTTCAAAGTAAAATCACTGACTTA2010.13074616380347745No Hit
CAGCCTGGGCGACAGAGCAAGACTCTGTCTCAAAAAAACAAATTACTATTTC1970.12814425009594557No Hit
AAAACGGGGAAGCCAACATATGAATATATGTTTTGTCTCGCTATACTGCACT1950.1268432932421796No Hit
GGTCTAGGGAAAGCTAGAGCCTCGCCCCACTCAAAAGAAGTACCATAAAGAG1860.12098898740023287No Hit
AAGTATGGGGGGGTTCATTAAACAGCAGAGAGGGAGTGAGTTCATGATCAGA1860.12098898740023287No Hit
AAGGCAGGAAGAGAAGTGTCAGGGAGTCAAAGATAGAGCTAAGGAAAGGCAA1840.11968803054646691No Hit
AAACAAGGGAGACAGTTTAGTGCTATATTATTTATTCAAGTACTTTTTATCA1820.11838707369270098No Hit
ATCAAAGGGGACAGAGCCGCGGCTTGTTTTCAGGACACGGGTCGCTGGCGGG1810.117736595265818No Hit
TTAACAGGGATTGCTCAGGTAACGAGAAAATAATAAAAGATGGAGAGAACAG1810.117736595265818No Hit
GAACCCAGGGAGAGAGAGAGAGATAGAGGAGAAAGGAGAGAAGAGAATGAGA1810.117736595265818No Hit
TTTAATGGGAATAAGAAACATTTTACAACCTGCTCTCTCTGAAGTCTGCTAT1800.11708611683893505No Hit
TAAAAAGGGAGATCGAAGGAAGCTATTTTTATCATATGAAAGCAAGTTTCAC1780.1157851599851691No Hit
ATTGTTGGGAAGAACTGTCAGGATTAGTTGGATGATAATTGACTAGTGTCTG1780.1157851599851691No Hit
ACTGCAGGGGCCCAACAAACTCCTGCGGATCACAGAGGATGGCACCTTGCTG1780.1157851599851691No Hit
TTCAAGGGGGTGACTGGGCATACCACATAATGTTTTTTAATTGATTTTGAAC1750.11383372470452016No Hit
TTGTCGGGGGGCCCGCAGCGCCGGAGTCAAAGCCGGTTCCCGGCCCAGTCCC1750.11383372470452016No Hit
GGTACGGGCTAATAAAATAGTGACAAGTAGAAATCTGGAGAATAATTCATAA1740.1131832462776372No Hit
TAGTGTGGGGGAGGAACAGTACCTTGGCTTGGCTCTTGACGTGGACAGAACT1710.11123181099698827No Hit
ATGCCGGGGAGCTTGACAGGCTCTGTTCCCTAGAGGTGTCCTCCTCTTTACT1700.11058133257010531No Hit
ACACATGGGGTGAACAATGGACCCCAAGTCATGTCAGTGTGGCCCCTGCTAC1690.10993085414322235No Hit
TGTAACGGGAGTTGACAATCTTCATGTGAATAATCCACTGAGTGGACTGATT1680.10928037571633936No Hit
CTGTTAGGGGTTTGTATTGGCTTGTTGCCTTTTCAGAGTTGTTGATGAGACT1630.10602798358192451No Hit
TTCGTAGGGGGAAGTGGTGAGAGAAGCTGGACTAGGCTTTGCTGTCAGTGTG1630.10602798358192451No Hit
GCTTTCCTTTCACTGGAATGTGCTCTTCGCTTTAGCCCTTTTTTTTTTTTTT1620.10537750515504153No Hit
AATTTTGGGAATTATACAGATGACATGTGCTTTTTTTTCTTTTTTCAACCTC1620.10537750515504153No Hit
ACACTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1620.10537750515504153No Hit
CCTGAGGGGGGAGAATAGCTTGGGCCTGGGAGGTGGAGGTTGCAGTGAGCCG1620.10537750515504153No Hit
TATGTTGGGGATGATTTCTAGTGACAACGACAGCATTTCTGAAGGGGTTATT1610.10472702672815856No Hit
GGTTGTGGGGGTTCATGGGATAAACAGCTGGTATTTGTATCTAAAACTCAGA1610.10472702672815856No Hit
CGAAGTGGGACCAAAGGACCTCATGGCTAATGCCACTTGCTTCAGGAGTGCC1600.10407654830127558No Hit
TGTCTCCCAGGCCATGCCACTGCTCTGCCACATGTCAGCAAATTTCTTTCCA1600.10407654830127558No Hit
TACTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1600.10407654830127558No Hit
TTGTTTGGGATGACTTGTTTTTATGAGTTTCAAGGTTTTCTTTTGTCTTTAA1600.10407654830127558No Hit
CGACTAGGGGACGCTCTTCCGATCTAAGGCTGGGGTTTTTCATTCTAAGATT1590.10342606987439262No Hit
GCCACAAATCCACTTTTTCTAAACCTATTAAGAACTAAGTATGTGTTGCATG1590.10342606987439262No Hit
TGCGCTGGGGACACTTATTTTTGATAGAGGTTCTTTGTTGCTTGTAAAGGGT1590.10342606987439262No Hit
GTTGTGGGGTAGCCTGGTGGGGTAGAAGGCAGAGAAGACTACCCAGCCAGTG1580.10277559144750964No Hit
GCAATTGGGAGAGGTTATTCTTCAGCATAGTGCTGCTGACATTGCCAGAGCT1580.10277559144750964No Hit
ATCGCAGGTACCATCGGGGTTTATGAATTTTTGCATTCTTTTGTTTATTGTA1580.10277559144750964No Hit
TCTACAGGGAGAATATATAGATTTTTCCATCATTCAACTAGTAAATTTTTAC1580.10277559144750964No Hit
ATATCTGGGAGTAGATTTAAGATTATAGAATATCAACAGGGATAAAAAAAGT1570.10212511302062667No Hit
CACCATGGGCTGAACTCTCGCTTTCTTTTTAATCCCCTGCATCGGATCACCG1570.10212511302062667No Hit
TACAGAGAAAGACTATGAAATACTTTTCAAATCTATTAATGGAATCCTTTTC1560.1014746345937437No Hit
TGAACTGGGGGGGAGGCTGCGGCGGCTGTAGCTGAGGTTTCACCCCTTTGGG1560.1014746345937437No Hit
TTCATGGGGCAGATGTAATTCACAAGCACATGGTGACAAATTGCCAGCTTTT1560.1014746345937437No Hit
AAATCAGGGATACTCTACTGATCCCAAATTGAAAGACGCACGAGAGATTTTA1550.10082415616686072No Hit
TCCATGGGGCTAGCCGTGGGTTCATGAATCTTGCTGTCAAGGAAGAAAGGTA1550.10082415616686072No Hit
AGATATGGGGACTCTCTTCCGATCTGATAATGGGAGCTTTCACCAGTTTTTA1550.10082415616686072No Hit
TTGAGAGGGTCTGGTGCAGGGGTGGAGGAAGATGGCTGCCGCCTGGGGGTCG1540.10017367773997776No Hit
ACTACGGGGGTATCAGGAAACTCAGAAGCAGTTTGCCTTGTCAAATTCAATC1540.10017367773997776No Hit
GCTACTGGGGGAAGACAGGGGCGATACAGCCCCTATGCCATTAATTGTAATA1540.10017367773997776No Hit
CATAGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1540.10017367773997776No Hit
GCACACGGGCCAGGTGCTGTTTATGTTGTAGGCACCTAAGTACTTATACAAG1540.10017367773997776No Hit
TTAGGGGGGAGGTGTCCTTTGACACACAGGCAGGTCAACCGCCCAGGACATC1540.10017367773997776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)