FastQCFastQC Report
Sat 31 Dec 2022
SRR5845121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153184
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGCCGTCT6540.42693753916858157No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGC6310.4119229162314602No Hit
AATGCCGGGGATGTACACAGCTGTGGGCTCTGAATGGCGTCCCTTTGGCTAT2350.1534102778358053No Hit
AAACAAGGGAGACAGTTTAGTGCTATATTATTTATTCAAGTACTTTTTATCA2200.1436181324420305No Hit
TAAGCTGGGCTTCTGATGGGACTGCACTTACGCTATCCAGTTGCAGGTAATT2060.13447879674117402No Hit
TTTAATGGGAATAAGAAACATTTTACAACCTGCTCTCTCTGAAGTCTGCTAT1900.12403384165448089No Hit
CGAAGTGGGACCAAAGGACCTCATGGCTAATGCCACTTGCTTCAGGAGTGCC1890.12338103196156258No Hit
CAGCCTGGGGCCAAGAGCGAGAGCCTGTCTCAACAATACCAACACCACCACC1880.12272822226864426No Hit
GGTACGGGCTAATAAAATAGTGACAAGTAGAAATCTGGAGAATAATTCATAA1860.12142260288280761No Hit
AATTTTGGGAATTATACAGATGACATGTGCTTTTTTTTCTTTTTTCAACCTC1860.12142260288280761No Hit
TGTAACGGGAGTTGACAATCTTCATGTGAATAATCCACTGAGTGGACTGATT1860.12142260288280761No Hit
TTAACAGGGATTGCTCAGGTAACGAGAAAATAATAAAAGATGGAGAGAACAG1860.12142260288280761No Hit
TGCGATGGGCACGTGAAGATGGATTTACTGTACGGTCTCGTGTGGCTGCTGA1840.12011698349697096No Hit
AAAACGGGGAAGCCAACATATGAATATATGTTTTGTCTCGCTATACTGCACT1830.11946417380405265No Hit
TAAAAAGGGAGATCGAAGGAAGCTATTTTTATCATATGAAAGCAAGTTTCAC1820.11881136411113433No Hit
TACCGGGGATCTATGTATTCAAGTAATTATTTTTTAGATAATAACTGTCTTC1820.11881136411113433No Hit
CACCATGGGCTGAACTCTCGCTTTCTTTTTAATCCCCTGCATCGGATCACCG1820.11881136411113433No Hit
AATGCTGGGGTGCATCTGATGTCCCTAATTTCAAAGTAAAATCACTGACTTA1810.118158554418216No Hit
AAGTATGGGGGGGTTCATTAAACAGCAGAGAGGGAGTGAGTTCATGATCAGA1800.11750574472529768No Hit
CAGCCTGGGCGACAGAGCAAGACTCTGTCTCAAAAAAACAAATTACTATTTC1780.11620012533946104No Hit
TGTCTCCCAGGCCATGCCACTGCTCTGCCACATGTCAGCAAATTTCTTTCCA1750.11424169626070607No Hit
AGAAGAGGGAGCGCTGTATTCATTTGAAGGACAGCAGCCTGGGGATTTGAAT1750.11424169626070607No Hit
ATGCCGGGGAGCTTGACAGGCTCTGTTCCCTAGAGGTGTCCTCCTCTTTACT1740.11358888656778775No Hit
ATTGTTGGGAAGAACTGTCAGGATTAGTTGGATGATAATTGACTAGTGTCTG1720.11228326718195111No Hit
ATCAAAGGGGACAGAGCCGCGGCTTGTTTTCAGGACACGGGTCGCTGGCGGG1720.11228326718195111No Hit
ACTGCAGGGGCCCAACAAACTCCTGCGGATCACAGAGGATGGCACCTTGCTG1720.11228326718195111No Hit
ACACATGGGGTGAACAATGGACCCCAAGTCATGTCAGTGTGGCCCCTGCTAC1700.11097764779611449No Hit
TTCGTAGGGGGAAGTGGTGAGAGAAGCTGGACTAGGCTTTGCTGTCAGTGTG1690.11032483810319617No Hit
ATAAGCGGGACACGAGCGCACACACACACTTAAGTGACTGTTAGTTCAGTGC1660.1083664090244412No Hit
TTGTTTGGGATGACTTGTTTTTATGAGTTTCAAGGTTTTCTTTTGTCTTTAA1660.1083664090244412No Hit
GTTGTGGGGTAGCCTGGTGGGGTAGAAGGCAGAGAAGACTACCCAGCCAGTG1650.10771359933152289No Hit
GAACCCAGGGAGAGAGAGAGAGATAGAGGAGAAAGGAGAGAAGAGAATGAGA1640.10706078963860456No Hit
ACTCATGGGATGTACTATATTACCAAGACATCCTTGCCACTTTTATGTCAAA1630.10640797994568624No Hit
TACATCGGGCTTTAAGCCTGAAAATAACTTTCCTGGTCATGGAAGAACTGGA1620.10575517025276791No Hit
CTTACAGGGCACACCAGTTATTCAATTGTCTGCTACCATGTATTGTGCACAT1610.10510236055984959No Hit
ATAGCAGGGAATCCCAATTATAAAATTCCACCTAAAGGAGTTTCATTTTGTA1600.10444955086693128No Hit
CTGTTAGGGGTTTGTATTGGCTTGTTGCCTTTTCAGAGTTGTTGATGAGACT1590.10379674117401295No Hit
TCGTTAGGGGCTGTTTGTAATTTCTCTCTTCTTGTCAAATTTAGATAAAGCT1590.10379674117401295No Hit
CGACTAGGGGACGCTCTTCCGATCTAAGGCTGGGGTTTTTCATTCTAAGATT1590.10379674117401295No Hit
GTCTCAGGGACCTTTCCGGAGGACAATTTAACTGATTTATTTAAAGCCCGAA1590.10379674117401295No Hit
CATAGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1580.10314393148109463No Hit
GCACACGGGCCAGGTGCTGTTTATGTTGTAGGCACCTAAGTACTTATACAAG1580.10314393148109463No Hit
TTGAGAGGGTCTGGTGCAGGGGTGGAGGAAGATGGCTGCCGCCTGGGGGTCG1570.1024911217881763No Hit
TTGTCGGGGGGCCCGCAGCGCCGGAGTCAAAGCCGGTTCCCGGCCCAGTCCC1560.10183831209525798No Hit
GATTAAGGGCAAATCATTGTGAAATATTAACACATCACTTATTTTTATAGCA1550.10118550240233966No Hit
TAGTGTGGGGGAGGAACAGTACCTTGGCTTGGCTCTTGACGTGGACAGAACT1550.10118550240233966No Hit
ATCATGGGGACAAATAGTTCAAATAACTATATATGACAATAATTGTGATAAA1540.10053269270942135No Hit
TGTTTGGGGCTGTCAACAACGTCAGTGTGGCATATTAACAAGACTAACTGCT1540.10053269270942135No Hit
TTGTACGGGATTTCATTCCGCTGGAACTAAAGGATAACTGCAGTGCTCATTT1540.10053269270942135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)