FastQCFastQC Report
Sat 31 Dec 2022
SRR5845118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223401
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGCCGTCT8650.38719611819105554No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGC8590.38451036477007716No Hit
GAACAGGGGAGACAATTAGAGTTTTCTAATTACACTTCCAGAATAAATCTTA2870.1284685386368011No Hit
GATTCGGGGACACCATTTTGTTCTACAGTCATTTACCTTAGCACTCCTATTG2710.12130652951419198No Hit
TTGTTTGGGCAAACACTGGATGCAGAAATTTTGGATTTTTCATCACTTTCTC2550.11414452039158285No Hit
CCTTACGGGGGGTTAGGGCTTCACTGAGTGAATTTTGGTGGAAAGTGGGGCA2430.10877301354962601No Hit
TTCGTTGGGGGAAGAAAATCCTGCCATTTGCAACAACATGGATGAATCTGAA2430.10877301354962601No Hit
CAAATAGGCCATTTCTATCTGTATCAATTGTATTAATTAGATAATGGTTAAA2420.10832538797946295No Hit
ACAGACGGGAGCAGAGTTTGGGTTGGTAAAAATAATGTTTGCTCTATTACTG2410.10787776240929986No Hit
TCCGCAGGGAAAAAAAGGGAATATCTGGTGTATGTACATGATGGAATACTAT2400.1074301368391368No Hit
TGAACTGGGGCCTGAAAGAAAGCATAAAGAAGTTCTAACTCTGAAACTAACT2380.10653488569881066No Hit
TAAAACGGGGGAAAGAGCTTGAGTTAGATTGAAGTAGAATCAGTGATAGAAA2370.1060872601286476No Hit
TAAGACCGGGGCTGAGGTTGAGTAAATACAGGTTTTTTACAACTCCTAAAGA2370.1060872601286476No Hit
AACATCGGGAATTCTTTTTGAGACAACCAGGGAAAATTGAAGACAGGTTAGG2330.1042967578479953No Hit
TTTGCGAGGGCAGACATAGAAGAACTCAAGAAAGAGACCTTTTGTGTTGTAG2320.10384913227783224No Hit
CGTTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2290.10250625556734302No Hit
TCAGCTGGGCAAAGAACAGCAGAGCTTGCACCCTGACTCAGCAGGCCTGAGA2290.10250625556734302No Hit
AATTGTGGGCTTGAATGAAGGGAAGTATAGCTGCCACAGCCCACGTATCTAT2280.10205862999717996No Hit
CTTTTGGGGAGAGCTGGGTCACGCACTTCCTAAGTTGTAGGATACCAGGCCA2270.10161100442701688No Hit
GCAGACAGCTTGATTTTAGCTAAGTGAAACCCATGGTACCAAAGTAGTTCAG2260.10116337885685382No Hit
AGTCTTGGGCTTTTCAGCCCAAATGTTTTTAACTTGATGGAATACAATTTAT2250.10071575328669076No Hit
TTGCTCCGGGTAAGTAGGATTTTCAAAACAAGGATCTTGAAGAACTAATCTG2240.10026812771652767No Hit
GAAAGGGGTGTAATACCGCATGTTAAACTCACAGAAGGTTTTTCTTGGAGAA2240.10026812771652767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)