FastQCFastQC Report
Sat 31 Dec 2022
SRR5845114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23126
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGC9384.056040819856439No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGCCGTCT9274.008475309175819No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGT2170.938337801608579No Hit
TCTTTGGGGAGACGGCAGGAGGCCTGAGCGATCCGTACTCAGAACGTCCAGG1650.7134826602092883No Hit
CAGTACGGGGGCTGGGCGAGGTGGCTCACGCCTGTAATCTCATCACTTTGGG1570.6788895615324743No Hit
CTGCATGGGATTGAAACTAATGAAATTACCAAGATGACAATGTCTTTTCTTT1420.6140275015134481No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTA1240.5361930294906166No Hit
TTGTCTGGGGGGACATCAGGGCCCTTCAGAAGGCACTGGGGATTGGAGCAGT1200.5188964801522096No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGT1130.4886275188099974No Hit
CCACTAGGACTAGGAGCTGTACAGCAGAACCCCTTCCCCTCAGAACTAATGC1090.4713309694715904No Hit
TCTTCGGGGGAGAATGGGCCATGATGACGATGGCGGTTTTGTAGAAAAAAAA1080.4670068321369887No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCCTGTCTCT980.42376545879097116No Hit
CTACCTGGGAAGCATCTCCCATAACAAATGATGGGGAAGATGAATTTGTTCC970.4194413214563694No Hit
GCCTAGGGGGCCCCTGCCACTGGGCCATGGAGACTGTGGCACAGTAGACTGT950.410793046787166No Hit
CTAACGGGGGAGAAACAGCAGTATGAATCTTGACGGTTTCTGCCCGTCCTAA900.38917236011415723No Hit
TACGCTGGGTGTAAGTTGTGAATTTCTCTCTTCAGAAATCTGACTTCCATTT860.37187581077575027No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCCTGTCTCTTATAC850.3675516734411485No Hit
TACATTGGGGATGTATGTGTCTATCTCTCCAGCAATTTCATACAGTCATGAT840.36322753610654673No Hit
ATTCAAGGGGGCTCCCGGGTGTGGTGGTCGCTCCAGCTCTCTGCTCTCCCAG830.358903398771945No Hit
AGTATGGGGCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCA830.358903398771945No Hit
GTAGATGGGAGGGAGAGAGTTTGGAAACCCCAACAGGCCTGTGGCCAGCACC830.358903398771945No Hit
ATTCTAGGGCAAAAAAATTATTTCTACACTGACAGGATTTACTAGTTAAGCA820.35457926143734325No Hit
CATACAAGGGGAAAATAATGCACATATGGTGTTCAAATTTGAACCTTTCTCA780.3372827120989363No Hit
AGATTTGGGAGAAATTTTCATACACTACTCTTAGGATTATAAAGTACAATTA720.3113378880913258No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTAT710.30701375075672405No Hit
AAGGCTGGGCAAGAATATTGTATGTTAAAGCATCAGAGATGGTAATCTACAG700.3026896134221223No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTAT660.2853930640837153No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGT650.28106892674911355No Hit
TGTGGTGGGAGGGGAGAGGCTGCCACGGCCCGACAAATGTCCCTGTGAGGTC620.2680965147453083No Hit
GGCATTGGGACTCACTAGGAAAGTTTTAAATACTGTTGTTTCTGTTACTTCA620.2680965147453083No Hit
TCAGCAAGGGACATGACTTTTTTTCTTGTCTTACTGTACCTTAAAAGGTGAT610.2637723774107066No Hit
TTAATAGGCACATTTGAACAGGGCAAAATGAACTAACTGCCATGTAGGCTAA610.2637723774107066No Hit
ACGCAAGGGGAAGCAGAGGCTTCCTAAGGCTGTTTTCTTAGCCGTGGAGAAG560.24215169073769782No Hit
TGTATGGGGATACCGTGTTCTTGGATAGCAAAGCTCCATATTGTTAAGATGT540.23350341606849434No Hit
CAGTGTGGGTCACTGACGTGGTCAGTTAGACATACGTTGGTTTCCAGGATGG520.22485514139929083No Hit
GCGTATGGGATATTTGAACACAGATTTGAAGGAGGTGAGAGAGTTAGCCAAA510.22053100406468912No Hit
GCCGCTGGGAAAGAGTTTTTAAGAACTATCTTCATCATCTTAGTATCAGATT500.21620686673008735No Hit
TACCTGGGATTTATCAGAGATGATTATTTTGTGCTACATACAGGTTGGCTAA480.20755859206088387No Hit
ATGTCGGGAGAACAATTCAAATAGGAGGAAAAACAAACTGCAGGAAACTGGA450.19458618005707862No Hit
AACCTGGGGACAGATGATTAAAGTTTATCAATTGTAAAGGTGGAGGAATTTG450.19458618005707862No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCG440.19026204272247688No Hit
GTATTTGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG430.18593790538787514No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATG430.18593790538787514No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTAACTCGTATGC390.16864135604946814No Hit
TACGGCGGGCTTGAAGGGAACTGTCACTACCCCCTCTAGGAAGTTATTTTAT380.16431721871486638No Hit
ATACTGGGGGATATCCCACCTGGAGCAGTATTAGAGAACAAGATTGTGTCTG370.15999308138026463No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCT360.1556689440456629No Hit
CTATTAGGGCTATTTTAATTGTTAGGAATTATTGGTTGTATGTGTTGCGAAT330.14269653204185764No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTC330.14269653204185764No Hit
CAGGTAGGGGGAATTGTGGTATTTTTTTCTGTATTGATTTTAACAGAAAATT310.13404825737265416No Hit
TGGAGCGGGACCTTCAAATGAAAAAAAATCTCAAAATTTCTATTTGGTTTTT310.13404825737265416No Hit
TAGCATGGGATTTCAGGTAGATATCAACAGATACTAAAACTAGTAGGAAAGT310.13404825737265416No Hit
TGATTTGGGGAGACTGTCTTTGCTTCATCATCTGAAGGTAAAATTTTCCAGA300.1297241200380524No Hit
TATGGGGGGAGCAGGAAAAGGGGAAGACAGGAATGTAAAACTACTAGATCAT300.1297241200380524No Hit
ACACGCGGGTTTTCCTCTTAGAAGAATATTGTATTGTTAGATGTTTGTTGAG290.12539998270345065No Hit
GAAAGGGGGCAAGAGTTTTCAGTAGAATATTTAAGAACTAATTTTCTGCAGC290.12539998270345065No Hit
ATTCCGGGGAGGGCCACTGAGCACTTGAAATGAGGCTAGTTCGTTTAGGAGG290.12539998270345065No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTC270.11675170803424717No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTACCTCGTATGCCGTCT270.11675170803424717No Hit
ACCCTTGGGGTAGACTCGCTCATCTCGCCTGGGTTTGTCCGCATGTTGTAAT260.11242757069964542No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTAACTCGTATGCCGTCT250.10810343336504367No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTACCTCGTATGC240.10377929603044193No Hit
GGCTATGGGAATATATCAGTAATTTCAGAATCAGATGCATCCTTTCATAAGA240.10377929603044193No Hit
GTCGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTC240.10377929603044193No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)