FastQCFastQC Report
Sat 31 Dec 2022
SRR5845113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22837
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGC8913.901563252616368No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGCCGTCT8573.7526820510574947No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGT2060.9020449270919998No Hit
TCTTTGGGGAGACGGCAGGAGGCCTGAGCGATCCGTACTCAGAACGTCCAGG1700.7444060077943687No Hit
CTGCATGGGATTGAAACTAATGAAATTACCAAGATGACAATGTCTTTTCTTT1330.5823882296273591No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGT1330.5823882296273591No Hit
GCCTAGGGGGCCCCTGCCACTGGGCCATGGAGACTGTGGCACAGTAGACTGT1260.5517362175417087No Hit
CAGTACGGGGGCTGGGCGAGGTGGCTCACGCCTGTAATCTCATCACTTTGGG1260.5517362175417087No Hit
TTGTCTGGGGGGACATCAGGGCCCTTCAGAAGGCACTGGGGATTGGAGCAGT1090.4772956167622718No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTA1030.4510224635459999No Hit
TACGCTGGGTGTAAGTTGTGAATTTCTCTCTTCAGAAATCTGACTTCCATTT1020.44664360467662134No Hit
CCACTAGGACTAGGAGCTGTACAGCAGAACCCCTTCCCCTCAGAACTAATGC1020.44664360467662134No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCCTGTCTCTTATAC1010.4422647458072426No Hit
CTAACGGGGGAGAAACAGCAGTATGAATCTTGACGGTTTCTGCCCGTCCTAA980.4291281691991067No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCCTGTCTCT950.41599159259097074No Hit
AGTATGGGGCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCA910.39847615711345624No Hit
CTACCTGGGAAGCATCTCCCATAACAAATGATGGGGAAGATGAATTTGTTCC900.39409729824407763No Hit
TCTTCGGGGGAGAATGGGCCATGATGACGATGGCGGTTTTGTAGAAAAAAAA880.3853395805053203No Hit
TACCTGGGATTTATCAGAGATGATTATTTTGTGCTACATACAGGTTGGCTAA860.376581862766563No Hit
ATTCAAGGGGGCTCCCGGGTGTGGTGGTCGCTCCAGCTCTCTGCTCTCCCAG830.36344528615842714No Hit
AGATTTGGGAGAAATTTTCATACACTACTCTTAGGATTATAAAGTACAATTA810.35468756841966986No Hit
GGCATTGGGACTCACTAGGAAAGTTTTAAATACTGTTGTTTCTGTTACTTCA800.3503087095502912No Hit
ATTCTAGGGCAAAAAAATTATTTCTACACTGACAGGATTTACTAGTTAAGCA800.3503087095502912No Hit
CATACAAGGGGAAAATAATGCACATATGGTGTTCAAATTTGAACCTTTCTCA800.3503087095502912No Hit
TACATTGGGGATGTATGTGTCTATCTCTCCAGCAATTTCATACAGTCATGAT770.33717213294215526No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTAT750.32841441520339804No Hit
GTAGATGGGAGGGAGAGAGTTTGGAAACCCCAACAGGCCTGTGGCCAGCACC730.3196566974646407No Hit
TGTGGTGGGAGGGGAGAGGCTGCCACGGCCCGACAAATGTCCCTGTGAGGTC710.31089897972588343No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGT710.31089897972588343No Hit
AAGGCTGGGCAAGAATATTGTATGTTAAAGCATCAGAGATGGTAATCTACAG670.2933835442483689No Hit
TCAGCAAGGGACATGACTTTTTTTCTTGTCTTACTGTACCTTAAAAGGTGAT650.2846258265096116No Hit
GCGTATGGGATATTTGAACACAGATTTGAAGGAGGTGAGAGAGTTAGCCAAA630.2758681087708543No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTAT620.27148924990147566No Hit
GCCGCTGGGAAAGAGTTTTTAAGAACTATCTTCATCATCTTAGTATCAGATT610.26711039103209705No Hit
GAAAGGGGGCAAGAGTTTTCAGTAGAATATTTAAGAACTAATTTTCTGCAGC580.2539738144239611No Hit
TGTATGGGGATACCGTGTTCTTGGATAGCAAAGCTCCATATTGTTAAGATGT570.2495949555545825No Hit
GTATTTGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG490.21456408459955334No Hit
TTAATAGGCACATTTGAACAGGGCAAAATGAACTAACTGCCATGTAGGCTAA490.21456408459955334No Hit
CAGTGTGGGTCACTGACGTGGTCAGTTAGACATACGTTGGTTTCCAGGATGG430.1882909313832815No Hit
AACCTGGGGACAGATGATTAAAGTTTATCAATTGTAAAGGTGGAGGAATTTG430.1882909313832815No Hit
ACGCAAGGGGAAGCAGAGGCTTCCTAAGGCTGTTTTCTTAGCCGTGGAGAAG410.17953321364452424No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTAACTCGTATGC410.17953321364452424No Hit
TAGCATGGGATTTCAGGTAGATATCAACAGATACTAAAACTAGTAGGAAAGT410.17953321364452424No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTC400.1751543547751456No Hit
ATGTCGGGAGAACAATTCAAATAGGAGGAAAAACAAACTGCAGGAAACTGGA390.17077549590576696No Hit
TACGGCGGGCTTGAAGGGAACTGTCACTACCCCCTCTAGGAAGTTATTTTAT390.17077549590576696No Hit
GACTTCGGGGGGATATCTGAGGCCATTACATTCCTGGGAATAACATGGTGAG350.1532600604282524No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATG350.1532600604282524No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCG330.1445023426894951No Hit
TGGAGCGGGACCTTCAAATGAAAAAAAATCTCAAAATTTCTATTTGGTTTTT320.1401234838201165No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCT320.1401234838201165No Hit
GTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCG300.1313657660813592No Hit
CAGGTAGGGGGAATTGTGGTATTTTTTTCTGTATTGATTTTAACAGAAAATT290.12698690721198055No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGCC290.12698690721198055No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTC290.12698690721198055No Hit
TGATTTGGGGAGACTGTCTTTGCTTCATCATCTGAAGGTAAAATTTTCCAGA280.12260804834260192No Hit
GCTTAAGGGTAGGAATGAAATCACACAGTGTCTTCTGTTTGCAGTATGTGAA270.11822918947322328No Hit
ACCCTTGGGGTAGACTCGCTCATCTCGCCTGGGTTTGTCCGCATGTTGTAAT260.11385033060384464No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTAT250.10947147173446599No Hit
ACACGCGGGTTTTCCTCTTAGAAGAATATTGTATTGTTAGATGTTTGTTGAG240.10509261286508736No Hit
ATACTGGGGGATATCCCACCTGGAGCAGTATTAGAGAACAAGATTGTGTCTG240.10509261286508736No Hit
TTATTCGGGGGTGCAGTCTTTGAGATAATTTGTTTTTACCTGTATTGCCCTT240.10509261286508736No Hit
TATGGGGGGAGCAGGAAAAGGGGAAGACAGGAATGTAAAACTACTAGATCAT240.10509261286508736No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)