FastQCFastQC Report
Sat 31 Dec 2022
SRR5845112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22982
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGC9183.994430423809938No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGCCGTCT8983.907405795840223No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGT2361.026890610042642No Hit
TCTTTGGGGAGACGGCAGGAGGCCTGAGCGATCCGTACTCAGAACGTCCAGG1740.7571142633365242No Hit
TTGTCTGGGGGGACATCAGGGCCCTTCAGAAGGCACTGGGGATTGGAGCAGT1310.570011313201636No Hit
CAGTACGGGGGCTGGGCGAGGTGGCTCACGCCTGTAATCTCATCACTTTGGG1190.5177965364198068No Hit
GCCTAGGGGGCCCCTGCCACTGGGCCATGGAGACTGTGGCACAGTAGACTGT1180.513445305021321No Hit
TCTTCGGGGGAGAATGGGCCATGATGACGATGGCGGTTTTGTAGAAAAAAAA1160.5047428422243495No Hit
ATTCTAGGGCAAAAAAATTATTTCTACACTGACAGGATTTACTAGTTAAGCA1140.4960403794273779No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTA1110.4829866852319206No Hit
CTAACGGGGGAGAAACAGCAGTATGAATCTTGACGGTTTCTGCCCGTCCTAA1090.4742842224349491No Hit
CTACCTGGGAAGCATCTCCCATAACAAATGATGGGGAAGATGAATTTGTTCC1080.4699329910364633No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGT1080.4699329910364633No Hit
CCACTAGGACTAGGAGCTGTACAGCAGAACCCCTTCCCCTCAGAACTAATGC1020.4438256026455487No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCCTGTCTCT1010.4394743712470629No Hit
CTGCATGGGATTGAAACTAATGAAATTACCAAGATGACAATGTCTTTTCTTT1010.4394743712470629No Hit
CATACAAGGGGAAAATAATGCACATATGGTGTTCAAATTTGAACCTTTCTCA970.42206944565311977No Hit
ATTCAAGGGGGCTCCCGGGTGTGGTGGTCGCTCCAGCTCTCTGCTCTCCCAG890.38725959446523367No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCCTGTCTCTTATAC860.37420590026977635No Hit
GGCATTGGGACTCACTAGGAAAGTTTTAAATACTGTTGTTTCTGTTACTTCA840.36550343747280484No Hit
TACGCTGGGTGTAAGTTGTGAATTTCTCTCTTCAGAAATCTGACTTCCATTT790.34374728048037595No Hit
GTAGATGGGAGGGAGAGAGTTTGGAAACCCCAACAGGCCTGTGGCCAGCACC760.33069358628491863No Hit
TGTGGTGGGAGGGGAGAGGCTGCCACGGCCCGACAAATGTCCCTGTGAGGTC750.3263423548864329No Hit
TACATTGGGGATGTATGTGTCTATCTCTCCAGCAATTTCATACAGTCATGAT750.3263423548864329No Hit
AGATTTGGGAGAAATTTTCATACACTACTCTTAGGATTATAAAGTACAATTA710.30893742929248974No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTAT670.2915325036985467No Hit
AAGGCTGGGCAAGAATATTGTATGTTAAAGCATCAGAGATGGTAATCTACAG660.2871812723000609No Hit
TACCTGGGATTTATCAGAGATGATTATTTTGTGCTACATACAGGTTGGCTAA650.28283004090157515No Hit
GCGTATGGGATATTTGAACACAGATTTGAAGGAGGTGAGAGAGTTAGCCAAA620.26977634670611783No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGT610.2654251153076321No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTAACTCGTATGC570.24802018971368894No Hit
AGTATGGGGCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCA560.2436689583152032No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTAT560.2436689583152032No Hit
TCAGCAAGGGACATGACTTTTTTTCTTGTCTTACTGTACCTTAAAAGGTGAT550.23931772691671743No Hit
ACGCAAGGGGAAGCAGAGGCTTCCTAAGGCTGTTTTCTTAGCCGTGGAGAAG540.23496649551823165No Hit
TTAATAGGCACATTTGAACAGGGCAAAATGAACTAACTGCCATGTAGGCTAA520.2262640327212601No Hit
CAGTGTGGGTCACTGACGTGGTCAGTTAGACATACGTTGGTTTCCAGGATGG510.22191280132277436No Hit
TGTATGGGGATACCGTGTTCTTGGATAGCAAAGCTCCATATTGTTAAGATGT500.2175615699242886No Hit
ATGTCGGGAGAACAATTCAAATAGGAGGAAAAACAAACTGCAGGAAACTGGA500.2175615699242886No Hit
GCCGCTGGGAAAGAGTTTTTAAGAACTATCTTCATCATCTTAGTATCAGATT480.20885910712731703No Hit
TGGAGCGGGACCTTCAAATGAAAAAAAATCTCAAAATTTCTATTTGGTTTTT430.18710295013488817No Hit
TACGGCGGGCTTGAAGGGAACTGTCACTACCCCCTCTAGGAAGTTATTTTAT430.18710295013488817No Hit
GAAAGGGGGCAAGAGTTTTCAGTAGAATATTTAAGAACTAATTTTCTGCAGC410.17840048733791664No Hit
AACCTGGGGACAGATGATTAAAGTTTATCAATTGTAAAGGTGGAGGAATTTG410.17840048733791664No Hit
GTATTTGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG400.17404925593943085No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCG390.1696980245409451No Hit
CAGGTAGGGGGAATTGTGGTATTTTTTTCTGTATTGATTTTAACAGAAAATT380.16534679314245931No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGCC370.16099556174397353No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTC340.14794186754851624No Hit
TGATTTGGGGAGACTGTCTTTGCTTCATCATCTGAAGGTAAAATTTTCCAGA340.14794186754851624No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATG330.14359063615003045No Hit
ACACGCGGGTTTTCCTCTTAGAAGAATATTGTATTGTTAGATGTTTGTTGAG310.13488817335305892No Hit
GACTTCGGGGGGATATCTGAGGCCATTACATTCCTGGGAATAACATGGTGAG310.13488817335305892No Hit
GTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCG300.13053694195457316No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCT290.12618571055608738No Hit
CTATTAGGGCTATTTTAATTGTTAGGAATTATTGGTTGTATGTGTTGCGAAT280.1218344791576016No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTACTGTCTCTTATACAC250.1087807849621443No Hit
ACCCTTGGGGTAGACTCGCTCATCTCGCCTGGGTTTGTCCGCATGTTGTAAT240.10442955356365852No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTC230.10007832216517275No Hit
GCTTAAGGGTAGGAATGAAATCACACAGTGTCTTCTGTTTGCAGTATGTGAA230.10007832216517275No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)