FastQCFastQC Report
Sat 31 Dec 2022
SRR5845093.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845093.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177155
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGCCGTCT21161.1944342524907567No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGC15730.887923005277864No Hit
AAGTTGGGGCGAATCACCGATCCCAGCAGCAGCAGGAAGGATCACTCGGTGA2600.14676413310377917No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGT2540.14337726849369198No Hit
TGTTGAGGGCGATCTACGTGGGGGAGATTTTTGTCTTAATTCAAAACCTGGT2510.14168383618864835No Hit
AAACCAGGGACCCAAAGGGTGACTTAGGAATTTAACTTCTAAATCTATTTAA2400.13547458440348847No Hit
TGCTTGGGGGCATATCTTCTTTCTTTCCTTTTCTTCTTCCTCAAAGGTATCT2360.13321667466343032No Hit
TTATGAGGGAAGCCTGGGAGAAGCTGACCCGGGAAGAGGGGACACCCTTGAC2340.13208771979340125No Hit
TCTTCAGGGAGTTTGACTTCCTCCCTTCCTATTCGAATGCATTTTATTTCTT2310.13039428748835766No Hit
TCATGAGGGTTCTTACCTAAACAGCATAATTTCTTGCTAAACAGAGAACATG2310.13039428748835766No Hit
AGTGGTGGGATCTTGTGGTATATAGAGATACATGCTGAAATGTCTACGGATG2300.12982981005334313No Hit
GCTAATGGGACCTTAATCTTTTATTATGCAAATGGGTTCTTTACCTGGCTGG2270.1281363777482995No Hit
TTTAATGGGCCCGGCGGCCGATGGCGGAGCAAGGCTCATCGCCGGTGCCAAA2180.12305608083316869No Hit
GGGGCAGGGAACGCAAGCCCTAATCCAGAACACCTGGTCTCCTGGGGATTCT2160.12192712596313963No Hit
CTTCATGGGGGATGAACGTAAGTAAGTGTCTCCTTGAGTTCTGTGAGCTACT2150.12136264852812509No Hit
CTTAACGGGATCACCAGTTCTACACAAACTCTTCGAGAAAATTGAAAAGGAG2110.11910473878806695No Hit
GTTTATGGGAACAATTAAAAGAACTAGAGAAGAAAGAGAAAACAAATTCAAA2100.1185402613530524No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCCTGTCTCTTATAC2070.1168468290480088No Hit
ACTGAGGGGTATCTTTTAAGTTTCAGAATCTAATTACACTCTTTGCAGAATT2070.1168468290480088No Hit
CAATACGGGGCTGAGGGGTGTGAGGCTCTAGTTGTGAAGAAGCTACAAGAAA2010.1134599644379216No Hit
TTGTAGGGGAATCAACAATTGATTTTTAAAAACTCATTTATTATAGCATCAA1970.11120205469786346No Hit
AATATGGGGGCCTGTGCTTATCAGAAAAGTGGTCTGCCTAAGGTCGTGCAGC1960.11063757726284891No Hit
GTAGACGGGACTGGTAGATGAGGAGCTGCAGAATTCTTAGCCTGGATTAGAG1960.11063757726284891No Hit
ATTGTTGGGGAGTTTTCCTAGAGACTCCTTAAATGGGGTCTGCGGCTTGGAG1940.10950862239281986No Hit
CACGTGGGGAGATGGGCCTGTGTGCACCCAACGTGATGCTATGCATGTCTGA1920.10837966752279078No Hit
GGTCCAGGGTGCGGTGGCCTCTGCTGGCTCTAGTCTTGTCTTCCTTGGCTCT1900.1072507126527617No Hit
TTGCATGGGGATATGGGAATGTGAAAGAAAATTGGTTGCAATAGTGTTTCTA1870.1055572803477181No Hit
GTAGTTGGGGTAACATGCCTTTATCAAACGGCTTACAGCTGTATTCCAACAC1840.1038638480426745No Hit
TGAAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1840.1038638480426745No Hit
CTTCCTGGGCGATCTATCGAAGGGAGTTATGGCTGGGGTGTGTGTGCGTGTG1840.1038638480426745No Hit
CACTAGGGGGAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGCCCGGGCG1820.10273489317264542No Hit
TTCAGGGGGGATTTGATGGAGCCCTGAATGTTGACCTGACAGAATTCCAGAC1790.10104146086760181No Hit

[WARN]Adapter Content

Adapter graph