FastQCFastQC Report
Sat 31 Dec 2022
SRR5845092.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845092.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177129
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGCCGTCT19311.0901659242698825No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGC14530.8203061045904397No Hit
AAACCAGGGACCCAAAGGGTGACTTAGGAATTTAACTTCTAAATCTATTTAA2700.15243127889842997No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGT2590.14622111568404947No Hit
GCTAATGGGACCTTAATCTTTTATTATGCAAATGGGTTCTTTACCTGGCTGG2510.14170463334631822No Hit
TGTTGAGGGCGATCTACGTGGGGGAGATTTTTGTCTTAATTCAAAACCTGGT2450.13831727159301976No Hit
ACTGAGGGGTATCTTTTAAGTTTCAGAATCTAATTACACTCTTTGCAGAATT2430.13718815100858694No Hit
TCTTCAGGGAGTTTGACTTCCTCCCTTCCTATTCGAATGCATTTTATTTCTT2340.13210710837863932No Hit
CTTCATGGGGGATGAACGTAAGTAAGTGTCTCCTTGAGTTCTGTGAGCTACT2270.12815518633312445No Hit
TCATGAGGGTTCTTACCTAAACAGCATAATTTCTTGCTAAACAGAGAACATG2260.12759062604090804No Hit
CTTAACGGGATCACCAGTTCTACACAAACTCTTCGAGAAAATTGAAAAGGAG2190.12363870399539319No Hit
CACTAGGGGGAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGCCCGGGCG2180.12307414370317678No Hit
AAGTTGGGGCGAATCACCGATCCCAGCAGCAGCAGGAAGGATCACTCGGTGA2150.12138046282652755No Hit
TGCTTGGGGGCATATCTTCTTTCTTTCCTTTTCTTCTTCCTCAAAGGTATCT2110.11912222165766193No Hit
AGTGGTGGGATCTTGTGGTATATAGAGATACATGCTGAAATGTCTACGGATG2090.1179931010732291No Hit
TTATGAGGGAAGCCTGGGAGAAGCTGACCCGGGAAGAGGGGACACCCTTGAC2080.1174285407810127No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCCTGTCTCTTATAC2050.1157348599043635No Hit
GTTTATGGGAACAATTAAAAGAACTAGAGAAGAAAGAGAAAACAAATTCAAA2030.11460573931993066No Hit
CACGTGGGGAGATGGGCCTGTGTGCACCCAACGTGATGCTATGCATGTCTGA2000.11291205844328146No Hit
TATCAAGGGTAACTATGCCCGAGGGCACTACACCATTGGCAAGGAGATCATT1990.11234749815106505No Hit
GTAGTTGGGGTAACATGCCTTTATCAAACGGCTTACAGCTGTATTCCAACAC1980.11178293785884864No Hit
AATGCCGGGATTCATATTGAGGCTTGATCCCCAATGTAATAGTGTTGAGAGG1950.1100892569821994No Hit
CAATACGGGGCTGAGGGGTGTGAGGCTCTAGTTGTGAAGAAGCTACAAGAAA1940.10952469668998299No Hit
ATTGTTGGGGTAGCAACCAATATCATGCTTTGAAGGAGTAATCTTCAGAGCC1930.10896013639776661No Hit
CTGTTGGGGAGTGTGGCTAGCATTTACAAAATCATGCTGAGTGGAGGCAGTG1910.10783101581333379No Hit
TTTAATGGGCCCGGCGGCCGATGGCGGAGCAAGGCTCATCGCCGGTGCCAAA1910.10783101581333379No Hit
CTTCAAGCGGGTCACCATGCTATGTGCTGTTGAATATGTAAAGCAACAAGAA1900.10726645552111738No Hit
CATACTGGGTGTACTTTCAATGGATTACATCTATGAAAACTAACTTGTTTAG1880.10613733493668456No Hit
GTAGACGGGACTGGTAGATGAGGAGCTGCAGAATTCTTAGCCTGGATTAGAG1850.10444365406003535No Hit
GAGGTGGGGGGTTCAGCCCACCGCCCGGCCAGCCGCCCCGTCCGGGAGGGAG1830.10331453347560253No Hit
TTGTAGGGGAATCAACAATTGATTTTTAAAAACTCATTTATTATAGCATCAA1810.10218541289116971No Hit
GGGGCAGGGAACGCAAGCCCTAATCCAGAACACCTGGTCTCCTGGGGATTCT1800.1016208525989533No Hit
ATCGGGGGACCCACATGCCTTGGAGTTCTTGAGAAAGGATTGCGCCAACGTC1800.1016208525989533No Hit
AACTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1800.1016208525989533No Hit
TTGCATGGGGATATGGGAATGTGAAAGAAAATTGGTTGCAATAGTGTTTCTA1800.1016208525989533No Hit
CTGTAAGGGATTCTAGACATGAAAATAAACCGTATGTTCCTAGTTCAGCACA1780.1004917320145205No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)