FastQCFastQC Report
Sat 31 Dec 2022
SRR5845091.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845091.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173663
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGCCGTCT20581.1850538111169333No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGC15280.8798650259410467No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGT2500.14395697413956918No Hit
TCATGAGGGTTCTTACCTAAACAGCATAATTTCTTGCTAAACAGAGAACATG2410.1387745230705447No Hit
GCTAATGGGACCTTAATCTTTTATTATGCAAATGGGTTCTTTACCTGGCTGG2360.1358953835877533No Hit
AAACCAGGGACCCAAAGGGTGACTTAGGAATTTAACTTCTAAATCTATTTAA2330.13416789989807845No Hit
GTTTATGGGAACAATTAAAAGAACTAGAGAAGAAAGAGAAAACAAATTCAAA2320.1335920720015202No Hit
TGTTGAGGGCGATCTACGTGGGGGAGATTTTTGTCTTAATTCAAAACCTGGT2230.1284096209324957No Hit
GGGGCAGGGAACGCAAGCCCTAATCCAGAACACCTGGTCTCCTGGGGATTCT2200.12668213724282087No Hit
AAGTTGGGGCGAATCACCGATCCCAGCAGCAGCAGGAAGGATCACTCGGTGA2200.12668213724282087No Hit
TGCTTGGGGGCATATCTTCTTTCTTTCCTTTTCTTCTTCCTCAAAGGTATCT2190.12610630934626257No Hit
GTAGTTGGGGTAACATGCCTTTATCAAACGGCTTACAGCTGTATTCCAACAC2000.11516557931165533No Hit
AGTGGTGGGATCTTGTGGTATATAGAGATACATGCTGAAATGTCTACGGATG2000.11516557931165533No Hit
TTTAATGGGCCCGGCGGCCGATGGCGGAGCAAGGCTCATCGCCGGTGCCAAA1990.11458975141509706No Hit
CTTCATGGGGGATGAACGTAAGTAAGTGTCTCCTTGAGTTCTGTGAGCTACT1980.11401392351853877No Hit
CTTAACGGGATCACCAGTTCTACACAAACTCTTCGAGAAAATTGAAAAGGAG1960.11286226772542222No Hit
TTATGAGGGAAGCCTGGGAGAAGCTGACCCGGGAAGAGGGGACACCCTTGAC1950.11228643982886397No Hit
CACTAGGGGGAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGCCCGGGCG1940.11171061193230568No Hit
CACGTGGGGAGATGGGCCTGTGTGCACCCAACGTGATGCTATGCATGTCTGA1930.11113478403574739No Hit
TTGCTAGGGATGTGGAATGGTTTAAAAGAAGTGTAAGGTACTGGTTGATTAA1920.11055895613918912No Hit
TTGCATGGGGATATGGGAATGTGAAAGAAAATTGGTTGCAATAGTGTTTCTA1890.10883147244951429No Hit
TCAATCGGGCGATCTACACTTGGGAGAGATTTGAAGGTGGATCCAAGCAGAA1880.10825564455295603No Hit
GCGCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1860.10710398875983945No Hit
ACTGAGGGGTATCTTTTAAGTTTCAGAATCTAATTACACTCTTTGCAGAATT1840.1059523329667229No Hit
TCTTCAGGGAGTTTGACTTCCTCCCTTCCTATTCGAATGCATTTTATTTCTT1830.10537650507016463No Hit
AATATGGGGGCCTGTGCTTATCAGAAAAGTGGTCTGCCTAAGGTCGTGCAGC1800.1036490213804898No Hit
CATACTGGGTGTACTTTCAATGGATTACATCTATGAAAACTAACTTGTTTAG1780.10249736558737325No Hit
CGTTAGGGGCTAGCATCCCAACCCTGGAGCTGTGCAGATTCTCAACAGCCTC1750.10076988189769841No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)