FastQCFastQC Report
Sat 31 Dec 2022
SRR5845090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266221
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGCCGTCT22330.8387768057365873No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGC16250.6103951228490615No Hit
GATATAGGGATGGTAGCTAGTAGCCACACTTGAACTGTAAGTAGTAAGACTG3690.13860664635772535No Hit
GCTATAGGGATTGCTTAATGAATTTAGAAAACATGTTTTCCTTAACTGACAT3500.13146971876749017No Hit
TGTAATGGGAGGACTCTTGGGAGGATTGTGAAGCTAATGGCACCTCCTTCCT3330.12508404671306922No Hit
GAAATAGGGGAGGCCTGATGCCTCTGGAGCTTTTTCCCTTTATAGCACCATT2920.10968330822887752No Hit
CCCCGTGGGGGGGACAGCAGAGGAGCAGTGTCCGGCCATGGCCGGAGCCCGC2830.10630265831771347No Hit
CGTCTAGGGATTCTATGCAAACTCACCATTCTTGGCAGTATACTTTCTCTCA2820.10592703054980637No Hit
TTTTGAAGGGGGAATTCAGGCTCAGAAGTGAACACTCCAGAACTTGCACCTG2810.10555140278189926No Hit
AATGCTGGGACTGGGAATTAATCCACATGTCTTACAGGTGAAGTTCTAACAC2730.10254638063864233No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)