FastQCFastQC Report
Sat 31 Dec 2022
SRR5845089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269530
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGCCGTCT21030.7802470967981301No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTAGTATCTCGTATGC16030.5947389900938671No Hit
GATATAGGGATGGTAGCTAGTAGCCACACTTGAACTGTAAGTAGTAAGACTG3540.1313397395466182No Hit
GCTATAGGGATTGCTTAATGAATTTAGAAAACATGTTTTCCTTAACTGACAT3530.13096872333320966No Hit
TTTTGAAGGGGGAATTCAGGCTCAGAAGTGAACACTCCAGAACTTGCACCTG3110.11538604237005157No Hit
CGTCTAGGGATTCTATGCAAACTCACCATTCTTGGCAGTATACTTTCTCTCA3030.11241791266278335No Hit
CCAGTTGGGAATACAGACAGCATAACATTGATTCCAAAAATGCCAGTATTGC2830.10499758839461285No Hit
TGTAATGGGAGGACTCTTGGGAGGATTGTGAAGCTAATGGCACCTCCTTCCT2830.10499758839461285No Hit
CCTGTTGGGAAACAGATCGCATATTATGATCTTGCTGCAGCCACAGTGCAGC2710.10054539383371054No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)