FastQCFastQC Report
Sat 31 Dec 2022
SRR5845054.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845054.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177588
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTCGCATATCTCGTATGCCGTCT13540.7624389035295178No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTCGCATATCTCGTATGC11800.6644593103137599No Hit
CTCATAGGGTACCTGTATTCACTGTAGAGAAGGAAAGACTTTTATCTTTCAC3830.2156677252967543No Hit
TAACAAGGGAGCGTGATGATGTGCCCATTTCTGGTTTGTTCACAGAGGAGGG2990.16836723202018153No Hit
ACATTCGGGACGCTTATCCTCTGTTAGCTTTTATGTTAGTATTGTAGTGAAA2840.1599207153636507No Hit
AAACAAGGGCGATCTCGAGAGGGGAATGGGAGACTTTAACACCCCACTGTCA2790.15710520981147374No Hit
GGTAATGGGTTTGCGAACGGCGAGCAGCGGCGGCGGCGCGGAGAGACGCAGC2710.15260040092799063No Hit
GAATAAGGGAACATTAGGATGATTTCTTCAATCTAACTGCATGTTTGTATCC2690.15147419870711984No Hit
TTTATGGGGAAGTACAAAGTGAGGAAGATGGAAGATGGTTGAATAGTGCTGA2670.15034799648624908No Hit
GTGCACGGTATCTGAAGAAATAATGGCTGAAAATGTCCTAATGTTGATGAAA2660.1497848953758137No Hit
CGCGAAGGGACGGTGGTTTGCGGTGTGGGAACGGCTGGAGGCGCGCGCAGCG2480.13964907538797666No Hit
GGCTGTGGGAACATCTCAAATACAACCACAGGAAAAATGGCCCAATCTGCCA2470.13908597427754127No Hit
ACAAAGGGGCTCTTGGGCAGAATCCCACAGAAGCAGAGTTACAGGACATGAT2460.13852287316710588No Hit
AAGACTGGGATGGCAAATATGGGGCCCAGAATGACAAAGGGGAGTGGAACGG2440.1373966709462351No Hit
CTTAATGGGATGTTAGGAAAGTCTGCCACCTTTTATCTTTTTTAAGGAATTT2420.13627046872536433No Hit
TGCTCAGGGATGGAGAAGTAATTCAGCTACAGGGTGACCAACGCAAGAACAT2420.13627046872536433No Hit
AAAATCGGGCTGTAATTGTGAATATTAACTGAGATAGTGAAACATGGTGTCC2420.13627046872536433No Hit
GTTTGAGGGCTGGGATTATAGGTGTGAGCCACTGCACCCAATCTCTGGTTAT2410.13570736761492894No Hit
TGTTAGGGGCATCCCCACCAGCCTCAGATGAGACGGGCCAGGACGCCCAGCC2400.13514426650449354No Hit
TTTAGCACGGGATGGCCTATTTTCTTGGTGTCATTGCCCACTTGGGGCCCTT2400.13514426650449354No Hit
TTACATGGGGCATTCTCGGGACCGTCCTTATCCCATTTAATTAATTTCTCTG2380.13401806428362276No Hit
ATAGCAGGGTACTTTTCTCTTAGCAGATAAATCTCACTGCTGTCATTTGATC2370.1334549631731874No Hit
TCACAGTGGGGAATGAAGAACAGATAGCTACTGTCTGCCTGTCAGTTCTGAG2360.132891862062752No Hit
CATTAAGGGGTCTCTGATAAATCAGGTAACAAAATGTATTTGATTGATGGAA2350.1323287609523166No Hit
TTGTCCAGGGCATTTAAATTGACTTCAGTGGTGAGACTGTATCCTGTTTGCT2340.1317656598418812No Hit
CTTAAAGGGGAGATGGGGCGAGGGCAGATGAAGGGCCCAGGAGCTTTGGGGC2330.13120255873144582No Hit
TGCACCGGGTGATGGTAGGCTTTGAACATGGGGTTGGCTGTTTCCCAGTAAA2330.13120255873144582No Hit
AGCGCCGGAGAGAATTCAAAAAGGGCTGATGGTAGGCTTTGAACATGGGGTT2320.13063945762101042No Hit
AATCATGGGGTAGACACAAAGTTAAACATTTCGACAGTCGGCTAGTCAAAAT2310.13007635651057503No Hit
GCTCATGGGGCCTAAAGTAAATTTAGATGTAAGAATATCGTCTTTATAGGTA2310.13007635651057503No Hit
CAAACAGGGGGAACATGGCGGCGCCCGTTGTCACGGCCCCGGGCAGAGCTCT2300.12951325540013964No Hit
GTGGCTGGGATATACTGCTTTAAAAAAGAATAAAAGAATATCCTTTTACAAG2290.12895015428970427No Hit
CTAATAGGGCAGGTGTCAGAGCTGAAATTCAAACCCCATCCAGCTGGCCCCG2280.12838705317926888No Hit
CGTAGCGGGAAAGGAAGATTGAAGAAGCACGAAGCCAAGCCAATCAGAAAGC2280.12838705317926888No Hit
TTATGAGGGTTGCGGCGGGATTTAAATATTTTTAAGAAAATAAAAAGCTTAA2260.1272608509583981No Hit
AGCAAGGGATAATTGGCATAGACATAAAGGATAAAAGGAAAATAACCGTCTG2250.1266977498479627No Hit
ACTGTGGGAAGCAAGAAAAAGAAGCCCTCAATCACCGCATCGTGCAGCAGGC2250.1266977498479627No Hit
TCTCGCGGGATTGAAAAAACTAGGTTGCTAACTTTTTTCATTCCTTATGCAA2250.1266977498479627No Hit
TGTCTAGGGATCTGTTGTTTCTAGAAAGTTCAGATCTAATTGTAATACTTGT2230.1255715476270919No Hit
GTTTTTGGGCAGTTGCCAGATGGATGAGGGAGTTTATCGCAAACCATCCTGA2230.1255715476270919No Hit
AAAGGTGGGGGCAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTCG2220.12500844651665652No Hit
CATAAAGGGAACCATGACAAACTTTTATTTTCTCTTTCTGATTTCCTAATGA2220.12500844651665652No Hit
GAATCCGGGGGAGCTTATTTCTTCAGACACCTAAAATTGTGGCAGATAAAGA2220.12500844651665652No Hit
GCCATGGCTTGATGTGAAGACAGCAAGGAAAGAAGCACCAGTCAAGTTGTGA2220.12500844651665652No Hit
GTTTCCGGGTTAAAGAGCGAGAGGAAGACCTGAGGCGTCTGTCTCGCCCGCT2200.12388224429578576No Hit
CAATTAGGGTGGTACTAATTACCTTTTCCTTGATGTTTGCCAAATTTGAATA2200.12388224429578576No Hit
ATTCGCGGGGGGCCATAAAAATATTCTACTAACATGAGGCTGATGTTCATAG2190.12331914318535037No Hit
GGGTACGGGTCCTTACTTTGAGCACATAGAACATCAAGAGGACTTAGGAAGC2180.12275604207491496No Hit
AAGCTTGGGGGGTCACGAACCCGTTACATTTCAGGACGATCCTTTTTCCTTC2180.12275604207491496No Hit
ATGCCAGGGATGAACAGCTCTTTTAATACAATTTCCTTGGTATCCAACTTAA2180.12275604207491496No Hit
TAATTTGGGAGGTACAGTTATTTGATTAGCAATTGTCTCCTCATTAATCTCT2180.12275604207491496No Hit
AACTTGGGGGAGCTGGTTATTGGTGTGGTCCTAGGGAGGGCGGAAAGGGAGG2180.12275604207491496No Hit
ATGCTAGGGCGTATATTGAACCAGCCTTGCATCCCAGGGATGAAGCCCACTT2170.12219294096447958No Hit
CCAATTGGGATGCACATTCATCCTCACATGTGGCACGTAAGGTCTCATTTGA2160.1216298398540442No Hit
CAAGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2160.1216298398540442No Hit
CACCAGGGGGGGCGACGCGCCGGTCTATGGCGTCAACACCGGCTTCGGCAAG2140.1205036376331734No Hit
TGTCTAGGGGTGGCGAGAGAAGCATGTCCGGTCACTCCGGGCCTGGCCACAT2140.1205036376331734No Hit
GCATCAGGGAACCTCTTACCCTGCAAGTTCAGAGACCCCTGGTCTCTGTGAC2130.11994053652273802No Hit
GTTCTCGGGCTTAGAAACTGGCTGGAAGAAGACAATGTGACCTGACTTAGGC2130.11994053652273802No Hit
CGTACTGGGCAGTGACATAGCAAGAATAAGTTAGTGTCTTTTTACTTTGTTA2130.11994053652273802No Hit
GTGTAAGGGATCCTGCTGATGTTGAGACAGTGGCTTTGTATAAGCTTTTATT2130.11994053652273802No Hit
AGCTTTGGGATAGGAAGTGAGATATAGGGGATAGTACTGTGCAATAAAATTG2130.11994053652273802No Hit
TTTGTTGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA2130.11994053652273802No Hit
TGCAGCGGGAACCACATAAATGAATTAAATAAACATTTTTAAATTTTCTTCA2130.11994053652273802No Hit
CTAGAGGGGGCAGAGATGAAAGACAGGACCCAGGGTCTCAGGATGCCTGTTG2110.11881433430186725No Hit
TCTGTTGGGAGAGGATGATTGCCCTGATTGACCTGGATGATTCTATTAAATA2110.11881433430186725No Hit
CTGGGGGGCTACAACCCTGGGCAAGTTACTTCACCTCTGAGCCTCCGATGCT2110.11881433430186725No Hit
TGATTAGGGGCTGTTTCCCAGTAAAACTGGAATTCCTGTCGTTACTGTTTCC2110.11881433430186725No Hit
ACTTTAGGGTATTGGCTGCTTTGCCTCTACCAGTTGGCAGACAGCCGTTTGT2100.11825123319143184No Hit
CGTGGGGTGAATCCATGGTGGGCCTTTTGCAAGTAGCGTTAGCTCTTTTTAA2100.11825123319143184No Hit
CTGACCGGGAGTTTTGTTCTTCTATGTATAGCTAGCTAGTTTTCCCAGCACC2080.11712503097056108No Hit
TCTTAAGGGTGTTGAAAGATTTGAAAGTGGTGCATTTTTACTTTTAAGAGCA2080.11712503097056108No Hit
TTTGAACGGGAAGCTAGGGGATGTCTATGTCAATGATGCTTTTGGCACTGCT2070.11656192986012569No Hit
GCATATGGGAGGGTTTTACTGTATTATTTTGAGGAGACACAATTCAACCCAT2070.11656192986012569No Hit
GTTCTAGGGGTGAAGAATCTCGTGGCACCAAGGTTAACTGCTTTCAAAAACT2060.11599882874969028No Hit
GCTATCGGGAGTATAGAAAATCCAAAGTTCAAATGGAACCTTGAGTCTTTTA2060.11599882874969028No Hit
ATAAGAGGGTGGGGCTCATCTCACAAGTTTCCTTACACTTAGGGATCAGTCT2050.1154357276392549No Hit
CGACACGGGTTCTGAACCTAGCCTATGTCTCTGTCCCCAAAATAGCTGCCCT2050.1154357276392549No Hit
ATCATGGGGTGTGAGTCAGTCAGCGAGTGCTTGGGATCCGCATCCAGCCGTG2050.1154357276392549No Hit
CAGATAGGGATTCCAAGTGTCATGTTGCTTTGTGGCAAGATTGTTGTGTGAC2040.11487262652881952No Hit
TCGTATGGGGAATTTTTTGGTGTCCCTTTTATCATAGGTGTTTAATGTATAT2040.11487262652881952No Hit
TCAGTGGGGGACGCTCTTCCGATCTTTCCGTAGGGATGTTGAAAACAGACAG2040.11487262652881952No Hit
AGAGCTGGGGGATCGAAGGCGAGATGGCGGACGTGCTAGATCTTCACGAGGC2040.11487262652881952No Hit
TTACCGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2040.11487262652881952No Hit
GCTAGGGGGAGCTTTTCCATTTTGCTCTGCTGCAGTAAATTGCAATTGCCAT2040.11487262652881952No Hit
TGTACAGGGGATCTTGATTGGGGTCTGCTTCAAGAGAAGCTGCACCTTTAAT2020.11374642430794875No Hit
CCAATTGGGATACTATTTTTTAAGATAGAAGATAATTTGTGGGCAGGGGTGG2020.11374642430794875No Hit
ATGTTGGGGAAACCTTTCCAACTTAGATGTCCTCAACCAAGTCATTAGAGAG2010.11318332319751334No Hit
AATCAAGGGCTTGTGTTCAGTGGATGAATACCTGTGGGCTCTTGTAATGTGT2000.11262022208707795No Hit
GTAAAAGGGACAACCACATGCAGAAGCACAAGCCCTACCCGTCCATCGAGGA2000.11262022208707795No Hit
CTGTCCGGGCATTCTATCTACTGATTTGGGCTGAATGTATGTAAATAGGTTT1990.11205712097664257No Hit
ACTGTCGGGAGACTTTAAGAGCTGAACGTTCTGGTTCTGGAAGCCATGTGAC1990.11205712097664257No Hit
AGCACAGGGGACAGTCTGAGTAAAATACCCAGTGATGTAGAAGTGTGCCTGT1980.11149401986620719No Hit
ACTATAGGGAGGAGTCCTCACCCCTCAGACTGCTGCAGGACATTGCCAGGCC1970.11093091875577178No Hit
CCATCGAGGGATGCACAGTCTACATGAAATCTTTGATCTCAATCTGTATATA1970.11093091875577178No Hit
CTCACAGGGTGGAAGGATGCCAGAGGAAGAAAAGGAAATGTGCAGAAGAAAA1970.11093091875577178No Hit
GGTAACGGGGCATCATGGCCTGAAAGCTCCACCAGCCTCTCCTGCCCTCCTC1960.11036781764533639No Hit
TCATCCGGGTACTGGAGAGAAGCCTTATGAATGTAGAGAATGTGGGAAAGCT1950.10980471653490101No Hit
CAGTCCGGGACTGTTTTTGTGGTAAGAACACTTTACATCCACTCTTAGTATT1950.10980471653490101No Hit
ATTGCCGGGACATCCTGATACATATCGATCAGTTGGTGCTGGGATTACAGGT1950.10980471653490101No Hit
TCTATTGGGGGGATAGTGCTTTTGTGAAATTTTTCAAATTTGAGTAATAGAT1940.10924161542446563No Hit
GACATAGGGAGGTATGGAGAAAATGAAAAATTACCAGACTACATCAAACAGA1930.10867851431403022No Hit
TCAATGGGGCTTCCCTTCCCTGCCCCTTTACAAATCCACCAGTCAGCAAGAG1930.10867851431403022No Hit
CGTCAGGGGTGGTTGAGCCCTGCTTTACTTATGTACTTGCTATCAGTATTTA1930.10867851431403022No Hit
AAAATGGGGAGCCATGGTCGTCTCCATTCCAGAGAGGTCATGACAAGACCAT1930.10867851431403022No Hit
GTCACCGGGGCCAACCAAGAAAATGAAGAAAAGGAGCAAGTTGCTAATAAAG1920.10811541320359483No Hit
TGTCGGGGGGAGTTGTTCGTTTTCCACTTGTATTTCCATTTTAATTATCATT1920.10811541320359483No Hit
AAAACAGGGATACACCTCTGGATGAGAAGAGCCTTGCTTTATTACTCAATTA1920.10811541320359483No Hit
GTCCGAGGGATTTGACTAGTCGAGTGATTTGCCCCAAGTTATACAACTTTAA1920.10811541320359483No Hit
TGTGATGGGAATTCCATCTGACTGCTTTGTTAAATTTTCAGTTAGAACGTAG1910.10755231209315945No Hit
TTACCTGGGAGCCCAGGATTGCTGAGTCGCTTGTGTGTCCGCTGTTTACAGC1910.10755231209315945No Hit
GTTGCAGGGAAAATTGTGCCAGCGAGCACCGTGAACGACTGCAATGCAAGCG1910.10755231209315945No Hit
AATAGTGGGAAGTTTAAAATTATTTCACAATTAAAAATTGAAAAGATTTGAC1910.10755231209315945No Hit
GTCATAGGGATAATATAATTTTAGTGCATTTCTCCTCATCAGGAATGCTGGA1890.10642610987228866No Hit
AAGTTAGGGACAGTTTTACAAGGATCTCATGGCAGAACAGCGGAAGACTTGG1890.10642610987228866No Hit
GTGACAGGGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCA1890.10642610987228866No Hit
TCAACCGGGTCTATCACCAGCAACAGAAGGTGCAGACTGTGTTGGTGGCGGT1890.10642610987228866No Hit
CGTAAAGGGGAGGAAGTTTACTGTCATGAGTCTCGGCTGGTTGGCGCATGTG1890.10642610987228866No Hit
GGTTCTGGGGTTACAGACCAGGCAGTTACTCCAGAAATACCTTTAAAAGGAG1880.10586300876185327No Hit
GTCTCAGGGCCTCCCAAGTATCTGGGATTACAGGCATGTGCCACCACGCCCA1880.10586300876185327No Hit
GGTGTGGGGGTTTATTTAAAGAAAAAATATGGTGCATTATCTTGTATCATTT1880.10586300876185327No Hit
ATTGTTGGGACTCCAGCCTGGGCGACAGAACGAGACCCTGTCTCCAAAGGAA1880.10586300876185327No Hit
AGTCCTGGGATGATCTGAAAAAGCAAATGCAGTTCATTTTTATGTATTTTTA1880.10586300876185327No Hit
ACCCGTGGGAAGGAACGCACTGTTCAAACGCACTGTTTACTTTAAGCGCACG1880.10586300876185327No Hit
GCTCAAAGGGGTAAATTATGGGCTATTCAAATGTAGACCATTTATAGTCTTT1880.10586300876185327No Hit
CTAGCAGGGGGGCAGCTCTTTTTAAGAGAAAAGCCAGAACCGGTGGAGCAGC1870.10529990765141789No Hit
AGTCAGAGGGATGCATGAAAAGTAAACATCAGAGACCTGAAGAAGCTTATAA1870.10529990765141789No Hit
GTGCTCGGGAATGATTTCATTAATAACACTTGTGATTTACTACCTTTTGATA1870.10529990765141789No Hit
GGCTGAGGGGATCTCTGAGTCGGAAGGGACTTACAAAGACCACCTGGTCCAC1870.10529990765141789No Hit
CAAATAGGGAGGGCAGAGAACCTGCCCTCCAGAATGTTATTGAGAGGAGCTG1860.1047368065409825No Hit
TGTTTTGGGGCTCTTCCGGTTCTAGGCGCTTCGGGAGCCGCGGCTTATGGTG1860.1047368065409825No Hit
AGACGCGGGCAGGATGGCATTGAGCTACAGGCAGGAGATGAGGTGGAGTTCT1860.1047368065409825No Hit
TTTCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1860.1047368065409825No Hit
GCTTATGGGCCATTCTGACTGGTGTGAGACAGTATCTAATTGTGGTTTTGAT1860.1047368065409825No Hit
GTAGGGGGGTGATCCATTTTCTAAGAAAGATTGGTATGATGTGAAAGCACCT1850.10417370543054712No Hit
ATCATTGGGTACGCTCAGACACTACAGCTGGGTTGGAATGGGCTTCTGGTGT1850.10417370543054712No Hit
GCTCTGGGGGGGTTGCATAAACAAGGCCTCACCATTCAAAAAACCTTGTATT1850.10417370543054712No Hit
AATCCAGGGATTTTCTCCAAGTTACCTATTCATGTATTAACAATTGATGCAA1850.10417370543054712No Hit
TAGCAGGGGAACTTCTTAATTTCTACATTCCCTCCCTTACTCTTCGGGGGTT1840.10361060432011171No Hit
CTACGAGGGCACATGAGCCTGAATATACTGTTGGGGTTCCCTGGTTGGATTC1840.10361060432011171No Hit
AAGAAAGGGGAACAAACTGGAGAATAAAATGGAAGGAATAGGTTTGAAGAGG1840.10361060432011171No Hit
TATACTGGGCAACCAGCGCTGCAAGAAGAGGATGAGGAAAATATAAAAGGAA1840.10361060432011171No Hit
AACGGCGGGATCATGGAGATTGAAGAGGAAAAGAAAAGAACTGCCCGAACAG1830.10304750320967633No Hit
TGTCCTGGGGGACTCATGTCATCCAGCGACAGTTCCACCAGCATCTCTTGTG1830.10304750320967633No Hit
GAATAGGGGTGTTCTTGGGTGCATACATGTTAAGGATAGTCATGTCTTCTTG1820.10248440209924094No Hit
TGACATGGGCAGCGTCTGCACATCCCTACAATGGCTAAAACAGCAATGGCCT1820.10248440209924094No Hit
GCTTCTGGGGATGAAAAAGACCTTTGGAGCAAAGCTGATGACAACTTCCCAG1820.10248440209924094No Hit
TCTTTAGGGGAGAACCTGTTTAAAATATTCTTAAATACTCTTCATTTTTACA1820.10248440209924094No Hit
AGCGTAGGGGGATTAATCTGGGGTTCACCTTCAAATATATAGAATAACACTT1810.10192130098880556No Hit
GACGTGGGTAACCAGGTAGACCAAATTCCGCTTTCAGTAATTCATCCATACA1810.10192130098880556No Hit
GTTTAAGGGGCCTTCTCCAGCAACCCCTTACTGCTGTATAACTTGCATAAGC1810.10192130098880556No Hit
GTTAATGGGATCAAGAAAGGCGCCAATGTCTCACCTGTGCTTGGGGTCAGGC1810.10192130098880556No Hit
AGTCGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1800.10135819987837015No Hit
GTAAGAGGGAGTTGCTGCTGCAGCTGAGGTACAGCGGCGGTTTCTGAGGTTC1790.10079509876793477No Hit
AATTAGGGGCGATCTGGTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCT1790.10079509876793477No Hit
AGCTCGGGGTAGTTTGTCCAGGGATGTTCATCTGTATACATGTGTATATGCA1780.10023199765749938No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)