FastQCFastQC Report
Sat 31 Dec 2022
SRR5845052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177089
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTCGCATATCTCGTATGCCGTCT12700.7171535216755417No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTCGCATATCTCGTATGC11780.6652022429399906No Hit
CTCATAGGGTACCTGTATTCACTGTAGAGAAGGAAAGACTTTTATCTTTCAC3890.21966355900140608No Hit
TAACAAGGGAGCGTGATGATGTGCCCATTTCTGGTTTGTTCACAGAGGAGGG3170.17900603651271393No Hit
ACATTCGGGACGCTTATCCTCTGTTAGCTTTTATGTTAGTATTGTAGTGAAA2910.16432415339179735No Hit
ATAGCAGGGTACTTTTCTCTTAGCAGATAAATCTCACTGCTGTCATTTGATC2900.1637594655794544No Hit
GAATAAGGGAACATTAGGATGATTTCTTCAATCTAACTGCATGTTTGTATCC2830.15980665089305376No Hit
AAGACTGGGATGGCAAATATGGGGCCCAGAATGACAAAGGGGAGTGGAACGG2730.1541597727696243No Hit
CGACACGGGTTCTGAACCTAGCCTATGTCTCTGTCCCCAAAATAGCTGCCCT2650.14964227027088076No Hit
TTTATGGGGAAGTACAAAGTGAGGAAGATGGAAGATGGTTGAATAGTGCTGA2600.146818831209166No Hit
TTACATGGGGCATTCTCGGGACCGTCCTTATCCCATTTAATTAATTTCTCTG2540.14343070433510832No Hit
GGCTGTGGGAACATCTCAAATACAACCACAGGAAAAATGGCCCAATCTGCCA2530.14286601652276537No Hit
GGTAATGGGTTTGCGAACGGCGAGCAGCGGCGGCGGCGCGGAGAGACGCAGC2440.13778382621167887No Hit
CGCGAAGGGACGGTGGTTTGCGGTGTGGGAACGGCTGGAGGCGCGCGCAGCG2430.13721913839933592No Hit
TGTTAGGGGCATCCCCACCAGCCTCAGATGAGACGGGCCAGGACGCCCAGCC2430.13721913839933592No Hit
CATTAAGGGGTCTCTGATAAATCAGGTAACAAAATGTATTTGATTGATGGAA2430.13721913839933592No Hit
TCTCGCGGGATTGAAAAAACTAGGTTGCTAACTTTTTTCATTCCTTATGCAA2400.1355250749623071No Hit
AAACAAGGGCGATCTCGAGAGGGGAATGGGAGACTTTAACACCCCACTGTCA2400.1355250749623071No Hit
AAAATCGGGCTGTAATTGTGAATATTAACTGAGATAGTGAAACATGGTGTCC2370.13383101152527824No Hit
AAGCTTGGGGGGTCACGAACCCGTTACATTTCAGGACGATCCTTTTTCCTTC2340.1321369480882494No Hit
CGTAAAGGGGAGGAAGTTTACTGTCATGAGTCTCGGCTGGTTGGCGCATGTG2340.1321369480882494No Hit
TGCTCAGGGATGGAGAAGTAATTCAGCTACAGGGTGACCAACGCAAGAACAT2320.1310075724635635No Hit
GTGCACGGTATCTGAAGAAATAATGGCTGAAAATGTCCTAATGTTGATGAAA2290.12931350902653468No Hit
AGCAAGGGATAATTGGCATAGACATAAAGGATAAAAGGAAAATAACCGTCTG2280.12874882121419173No Hit
CCAATTGGGATGCACATTCATCCTCACATGTGGCACGTAAGGTCTCATTTGA2270.12818413340184878No Hit
CTGGGGGGCTACAACCCTGGGCAAGTTACTTCACCTCTGAGCCTCCGATGCT2270.12818413340184878No Hit
GTAAAGGGGCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGG2260.12761944558950586No Hit
CTTAAAGGGGAGATGGGGCGAGGGCAGATGAAGGGCCCAGGAGCTTTGGGGC2240.12649006996481993No Hit
TTACCGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2240.12649006996481993No Hit
ACTTTAGGGTATTGGCTGCTTTGCCTCTACCAGTTGGCAGACAGCCGTTTGT2240.12649006996481993No Hit
TCACAGTGGGGAATGAAGAACAGATAGCTACTGTCTGCCTGTCAGTTCTGAG2240.12649006996481993No Hit
GCCATGGCTTGATGTGAAGACAGCAAGGAAAGAAGCACCAGTCAAGTTGTGA2240.12649006996481993No Hit
TTTAGCACGGGATGGCCTATTTTCTTGGTGTCATTGCCCACTTGGGGCCCTT2230.125925382152477No Hit
GTGACAGGGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCA2230.125925382152477No Hit
AATAGTGGGAAGTTTAAAATTATTTCACAATTAAAAATTGAAAAGATTTGAC2220.12536069434013405No Hit
AAAGGTGGGGGCAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTCG2210.12479600652779112No Hit
CTAGAGGGGGCAGAGATGAAAGACAGGACCCAGGGTCTCAGGATGCCTGTTG2200.12423131871544817No Hit
TTACCTGGGAGCCCAGGATTGCTGAGTCGCTTGTGTGTCCGCTGTTTACAGC2200.12423131871544817No Hit
ATGTTGGGGAAACCTTTCCAACTTAGATGTCCTCAACCAAGTCATTAGAGAG2200.12423131871544817No Hit
TTGTCCAGGGCATTTAAATTGACTTCAGTGGTGAGACTGTATCCTGTTTGCT2190.12366663090310523No Hit
AATCATGGGGTAGACACAAAGTTAAACATTTCGACAGTCGGCTAGTCAAAAT2180.12310194309076228No Hit
GTAGGGGGGTGATCCATTTTCTAAGAAAGATTGGTATGATGTGAAAGCACCT2180.12310194309076228No Hit
CATAAAGGGAACCATGACAAACTTTTATTTTCTCTTTCTGATTTCCTAATGA2180.12310194309076228No Hit
TTTGGCGGGGATGAGTTGAGCTCTATCTTCATGTACTCATCCTGAATCCTTA2170.12253725527841931No Hit
AGCGCCGGAGAGAATTCAAAAAGGGCTGATGGTAGGCTTTGAACATGGGGTT2170.12253725527841931No Hit
CAATTAGGGTGGTACTAATTACCTTTTCCTTGATGTTTGCCAAATTTGAATA2170.12253725527841931No Hit
TCAGTGGGGGACGCTCTTCCGATCTTTCCGTAGGGATGTTGAAAACAGACAG2170.12253725527841931No Hit
CGTAGCGGGAAAGGAAGATTGAAGAAGCACGAAGCCAAGCCAATCAGAAAGC2170.12253725527841931No Hit
GTGTAAGGGATCCTGCTGATGTTGAGACAGTGGCTTTGTATAAGCTTTTATT2160.12197256746607638No Hit
GCTAGGGGGAGCTTTTCCATTTTGCTCTGCTGCAGTAAATTGCAATTGCCAT2150.12140787965373342No Hit
TTATGAGGGTTGCGGCGGGATTTAAATATTTTTAAGAAAATAAAAAGCTTAA2150.12140787965373342No Hit
CTAGCAGGGGGGCAGCTCTTTTTAAGAGAAAAGCCAGAACCGGTGGAGCAGC2140.12084319184139049No Hit
CGTGGGGTGAATCCATGGTGGGCCTTTTGCAAGTAGCGTTAGCTCTTTTTAA2140.12084319184139049No Hit
CTGTCCGGGCATTCTATCTACTGATTTGGGCTGAATGTATGTAAATAGGTTT2130.12027850402904755No Hit
GTTTCCGGGTTAAAGAGCGAGAGGAAGACCTGAGGCGTCTGTCTCGCCCGCT2120.1197138162167046No Hit
TGTGATGGGAATTCCATCTGACTGCTTTGTTAAATTTTCAGTTAGAACGTAG2100.1185844405920187No Hit
GTTCTCGGGCTTAGAAACTGGCTGGAAGAAGACAATGTGACCTGACTTAGGC2100.1185844405920187No Hit
CAAAAAGGGTTCTACCACCAGCCTCCTAAAAATAATTTTATTCAGTAAAAAT2100.1185844405920187No Hit
GTTTGAGGGCTGGGATTATAGGTGTGAGCCACTGCACCCAATCTCTGGTTAT2100.1185844405920187No Hit
GGTTCTGGGGTTACAGACCAGGCAGTTACTCCAGAAATACCTTTAAAAGGAG2090.11801975277967575No Hit
GCTCATGGGGCCTAAAGTAAATTTAGATGTAAGAATATCGTCTTTATAGGTA2090.11801975277967575No Hit
GTCACCGGGGCCAACCAAGAAAATGAAGAAAAGGAGCAAGTTGCTAATAAAG2080.11745506496733281No Hit
AGTCAGAGGGATGCATGAAAAGTAAACATCAGAGACCTGAAGAAGCTTATAA2080.11745506496733281No Hit
ACAAAGGGGCTCTTGGGCAGAATCCCACAGAAGCAGAGTTACAGGACATGAT2080.11745506496733281No Hit
AGAGCTGGGGGATCGAAGGCGAGATGGCGGACGTGCTAGATCTTCACGAGGC2070.11689037715498986No Hit
ATTCGCGGGGGGCCATAAAAATATTCTACTAACATGAGGCTGATGTTCATAG2060.11632568934264692No Hit
TAATTTGGGAGGTACAGTTATTTGATTAGCAATTGTCTCCTCATTAATCTCT2060.11632568934264692No Hit
ATGCCAGGGATGAACAGCTCTTTTAATACAATTTCCTTGGTATCCAACTTAA2050.11576100153030397No Hit
GTCCGAGGGATTTGACTAGTCGAGTGATTTGCCCCAAGTTATACAACTTTAA2050.11576100153030397No Hit
ATTGCCGGGACATCCTGATACATATCGATCAGTTGGTGCTGGGATTACAGGT2050.11576100153030397No Hit
TGTCTAGGGATCTGTTGTTTCTAGAAAGTTCAGATCTAATTGTAATACTTGT2040.11519631371796103No Hit
AACTTGGGGGAGCTGGTTATTGGTGTGGTCCTAGGGAGGGCGGAAAGGGAGG2040.11519631371796103No Hit
CAAGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2030.1146316259056181No Hit
AACGGCGGGATCATGGAGATTGAAGAGGAAAAGAAAAGAACTGCCCGAACAG2030.1146316259056181No Hit
GTTGCAGGGAAAATTGTGCCAGCGAGCACCGTGAACGACTGCAATGCAAGCG2030.1146316259056181No Hit
GCATATGGGAGGGTTTTACTGTATTATTTTGAGGAGACACAATTCAACCCAT2030.1146316259056181No Hit
AAAACAGGGATACACCTCTGGATGAGAAGAGCCTTGCTTTATTACTCAATTA2030.1146316259056181No Hit
GGGTACGGGTCCTTACTTTGAGCACATAGAACATCAAGAGGACTTAGGAAGC2020.11406693809327513No Hit
AGCCTAGGGGCATGGATCTTTGCCTAATACTCAGCTTGAACATTTTTATATA2020.11406693809327513No Hit
CAGATAGGGATTCCAAGTGTCATGTTGCTTTGTGGCAAGATTGTTGTGTGAC2010.11350225028093218No Hit
AAGTTAGGGACAGTTTTACAAGGATCTCATGGCAGAACAGCGGAAGACTTGG2010.11350225028093218No Hit
TGCACCGGGTGATGGTAGGCTTTGAACATGGGGTTGGCTGTTTCCCAGTAAA2010.11350225028093218No Hit
AATCAAGGGCTTGTGTTCAGTGGATGAATACCTGTGGGCTCTTGTAATGTGT2000.11293756246858924No Hit
ACTGTGGGAAGCAAGAAAAAGAAGCCCTCAATCACCGCATCGTGCAGCAGGC2000.11293756246858924No Hit
AAAATGGGGAGCCATGGTCGTCTCCATTCCAGAGAGGTCATGACAAGACCAT2000.11293756246858924No Hit
TGTCCTGGGGGACTCATGTCATCCAGCGACAGTTCCACCAGCATCTCTTGTG1990.11237287465624629No Hit
TCAACCGGGTCTATCACCAGCAACAGAAGGTGCAGACTGTGTTGGTGGCGGT1990.11237287465624629No Hit
GCTTCTGGGGATGAAAAAGACCTTTGGAGCAAAGCTGATGACAACTTCCCAG1990.11237287465624629No Hit
ATAAGAGGGTGGGGCTCATCTCACAAGTTTCCTTACACTTAGGGATCAGTCT1980.11180818684390335No Hit
CTGACCGGGAGTTTTGTTCTTCTATGTATAGCTAGCTAGTTTTCCCAGCACC1980.11180818684390335No Hit
TAGGATGGGGATGTCATATGTGAACTAGAAGTACGTGTTACTGGCCAAGGCT1980.11180818684390335No Hit
TTTGAACGGGAAGCTAGGGGATGTCTATGTCAATGATGCTTTTGGCACTGCT1980.11180818684390335No Hit
GCTCTGGGGGGGTTGCATAAACAAGGCCTCACCATTCAAAAAACCTTGTATT1980.11180818684390335No Hit
TCAATGGGGCTTCCCTTCCCTGCCCCTTTACAAATCCACCAGTCAGCAAGAG1980.11180818684390335No Hit
GCTTATGGGCCATTCTGACTGGTGTGAGACAGTATCTAATTGTGGTTTTGAT1960.11067881121921747No Hit
GACGTGGGTAACCAGGTAGACCAAATTCCGCTTTCAGTAATTCATCCATACA1960.11067881121921747No Hit
GTTTAAGGGGCCTTCTCCAGCAACCCCTTACTGCTGTATAACTTGCATAAGC1950.1101141234068745No Hit
TGTACAGGGGATCTTGATTGGGGTCTGCTTCAAGAGAAGCTGCACCTTTAAT1940.10954943559453156No Hit
TTTCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1930.10898474778218861No Hit
TCTTACCGGGGGGGAGGCGTGCGGCTCAGCGGTCGGCTCGACATGGGACGTC1930.10898474778218861No Hit
ACTGTCGGGAGACTTTAAGAGCTGAACGTTCTGGTTCTGGAAGCCATGTGAC1920.10842005996984568No Hit
TCTTTAGGGGAGAACCTGTTTAAAATATTCTTAAATACTCTTCATTTTTACA1920.10842005996984568No Hit
GAATCCGGGGGAGCTTATTTCTTCAGACACCTAAAATTGTGGCAGATAAAGA1920.10842005996984568No Hit
GTTTTTGGGCAGTTGCCAGATGGATGAGGGAGTTTATCGCAAACCATCCTGA1910.10785537215750272No Hit
CTCACAGGGTGGAAGGATGCCAGAGGAAGAAAAGGAAATGTGCAGAAGAAAA1910.10785537215750272No Hit
AGCTTTGGGATAGGAAGTGAGATATAGGGGATAGTACTGTGCAATAAAATTG1910.10785537215750272No Hit
CTAATAGGGCAGGTGTCAGAGCTGAAATTCAAACCCCATCCAGCTGGCCCCG1910.10785537215750272No Hit
AGTTGAGGGTATGGATGTTGTAGTGCTCAACTGATGCTTCCATTCTATACCC1900.10729068434515979No Hit
CTTAATGGGATGTTAGGAAAGTCTGCCACCTTTTATCTTTTTTAAGGAATTT1900.10729068434515979No Hit
TGCAGCGGGAACCACATAAATGAATTAAATAAACATTTTTAAATTTTCTTCA1890.10672599653281684No Hit
TCGTATGGGGAATTTTTTGGTGTCCCTTTTATCATAGGTGTTTAATGTATAT1880.10616130872047387No Hit
CACTATGGGCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAA1880.10616130872047387No Hit
AAGTGTGGAGGAATAGATCTGAATAAAGCAAATCTGCATAAATGGTAACCAG1880.10616130872047387No Hit
GAATACGCGGGAAACCAAATCCAGAAAGCTTAAACAATAGAGCTGCATAATA1870.10559662090813093No Hit
TGTACGCGGGGCAGGAAGAAGTATTTCGAAATTTAGATGCTCAGTATGAAAT1870.10559662090813093No Hit
GCATTTGGGAGTGGAGCCTTTCAGTGAAATAGGTGACTATTAAAAAAAGTAA1870.10559662090813093No Hit
CGTCAGGGGTGGTTGAGCCCTGCTTTACTTATGTACTTGCTATCAGTATTTA1870.10559662090813093No Hit
AGCGTAGGGGGATTAATCTGGGGTTCACCTTCAAATATATAGAATAACACTT1860.10503193309578798No Hit
AGTCCTGGGATGATCTGAAAAAGCAAATGCAGTTCATTTTTATGTATTTTTA1860.10503193309578798No Hit
AGCCTGGGGAAGAGACAGAATAAGCATCAGAACCAGACTCATATATGGCAGT1860.10503193309578798No Hit
AATCCAGGGATTTTCTCCAAGTTACCTATTCATGTATTAACAATTGATGCAA1860.10503193309578798No Hit
TCTGTTGGGAGAGGATGATTGCCCTGATTGACCTGGATGATTCTATTAAATA1850.10446724528344505No Hit
ATCATGGGGTGTGAGTCAGTCAGCGAGTGCTTGGGATCCGCATCCAGCCGTG1850.10446724528344505No Hit
TCTTAAGGGTGTTGAAAGATTTGAAAGTGGTGCATTTTTACTTTTAAGAGCA1850.10446724528344505No Hit
GTGGCTGGGATATACTGCTTTAAAAAAGAATAAAAGAATATCCTTTTACAAG1840.10390255747110211No Hit
TAGCAGGGGAACTTCTTAATTTCTACATTCCCTCCCTTACTCTTCGGGGGTT1840.10390255747110211No Hit
TACGCCGGGATAGAAGTAGAAGTAGGAGCCGAGATAAATCCCATAGGCATAA1830.10333786965875916No Hit
ACCCTTGGGTCTTTCCAAAGAGAGCAGGACAAACAGGACAATAAACTGCAGC1830.10333786965875916No Hit
AGACGAGGGATGATAATAGAATAATTTGCACTAATTATTGTAAATATGTCTA1830.10333786965875916No Hit
GTAAAAGGGACAACCACATGCAGAAGCACAAGCCCTACCCGTCCATCGAGGA1830.10333786965875916No Hit
ATGCTAGGGCGTATATTGAACCAGCCTTGCATCCCAGGGATGAAGCCCACTT1830.10333786965875916No Hit
ATTGTTGGGACTCCAGCCTGGGCGACAGAACGAGACCCTGTCTCCAAAGGAA1820.10277318184641622No Hit
CTAGAAGGGGATCAACAGAGTGACAAGACAACTTGCAGAATCAGAGAAAATA1820.10277318184641622No Hit
GATTATGGGGATCAAAAGGAAATGAGCAAAATGTAAATAGAGGGGATATGTA1820.10277318184641622No Hit
GCTTTAGGGCTTTACACTAATAACCTCAACTGTTTCTTCTTCTCAGAGCCTA1820.10277318184641622No Hit
TGATTAGGGGCTGTTTCCCAGTAAAACTGGAATTCCTGTCGTTACTGTTTCC1820.10277318184641622No Hit
ACTATAGGGAGGAGTCCTCACCCCTCAGACTGCTGCAGGACATTGCCAGGCC1810.10220849403407327No Hit
TGTGTCGGGCTAAATGAGACAACAAAGCACAATGTTCACTGTTTACAACCAA1810.10220849403407327No Hit
TCTATTGGGGGGATAGTGCTTTTGTGAAATTTTTCAAATTTGAGTAATAGAT1810.10220849403407327No Hit
CTTGATGGGTGATAACTCTTGTATCCTGGGTTAAAGCCCTCTGTATTTAGTT1810.10220849403407327No Hit
CAAATAGGGAGGGCAGAGAACCTGCCCTCCAGAATGTTATTGAGAGGAGCTG1800.1016438062217303No Hit
TGTCGGGGGGAGTTGTTCGTTTTCCACTTGTATTTCCATTTTAATTATCATT1800.1016438062217303No Hit
TTTGTTGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1800.1016438062217303No Hit
GAATTCGGGACAAGTGAAAGCAGATTTTGTCATTTTATTTGGCCTTTGCTTG1800.1016438062217303No Hit
CCGTTCGGGCAGATGGTGCTGAAATCTATTACCTACATGTTTTCTAGTTGTT1800.1016438062217303No Hit
GGTAACGGGGCATCATGGCCTGAAAGCTCCACCAGCCTCTCCTGCCCTCCTC1800.1016438062217303No Hit
ACGTCAGGGTACGTGTTACTGGCCAAGGCTATTTTTCAGAACTGTTAAAGGT1800.1016438062217303No Hit
GCATCAGGGAACCTCTTACCCTGCAAGTTCAGAGACCCCTGGTCTCTGTGAC1790.10107911840938737No Hit
GCAAAAGGGGTTAACAACAGAATTCCACACAGTGCTGCAATTCAATTTTACA1790.10107911840938737No Hit
GTTTTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1780.10051443059704442No Hit
CAGTCCGGGACTGTTTTTGTGGTAAGAACACTTTACATCCACTCTTAGTATT1780.10051443059704442No Hit
GGCTGAGGGGATCTCTGAGTCGGAAGGGACTTACAAAGACCACCTGGTCCAC1780.10051443059704442No Hit
CGTTACGGGGTGAATTTAGTCATTTTACAATATGTTTGTATTTGGCCATTTA1780.10051443059704442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)