FastQCFastQC Report
Sat 31 Dec 2022
SRR5845039.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845039.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139942
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTCGCATATCTCGTATGC8670.6195423818439068No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTCGCATATCTCGTATGCCGTCT7870.5623758414200168No Hit
CGTACAGGGTAATTTGACATTCTAGAATTGTTAATTTTAGATAAAACCTCCT3290.2350973974932472No Hit
TCAGCGGGTGGGGAGAATGTGACATTATAGAAATAAGAGAAAATCAGAAATT3070.2193765988766775No Hit
TCAGAATGGGAGAAAATATTTGCTTAACACTAAACAAGGCAGTAAAGAAGAA2390.17078503951637106No Hit
TAGCAAGGGCAAGTTTACCTTTTTGAAAGAAGTTAAACCAATAATTTATTTT2360.1686412942504752No Hit
AGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG2150.1536350773892041No Hit
TGAAATGGGGGCATCAAGGTAGACAAGGGCGTGGTCCCCCTGGCAGGGACAA2140.15292049563390547No Hit
CCGCTGGGATTACAGGCGTGAGCCACCGCACCTGGCCTGCCTGTTTTACCTT2110.1507767503680096No Hit
AGCCTGGGTGACAGAGCAAGGTGCTATCTCCAAAATAAATAAATAAATGTTA2050.14648925983621786No Hit
AGATACGGGGAGGCGAAGATGGTGCACATACCCAGATATCTGAGTCAGGGAA2040.14577467808091923No Hit
GCTTGGGGGCGGCCACTTATTCGTGATGCCAACAATTTTGTATTAGATGAAC1990.1422017693044261No Hit
TTGCTCGGGAATATTCTTACTCTTAGTATTAAATAATTTTATGAGTAAAAGA1860.132912206485544No Hit
GAATCTGGGATATTGGGTTTTAAAAAAGTGTATTGTGACTTAAACTGTTATA1840.13148304297494676No Hit
TACACAGGGAGCACAGGCACACTTAATACATTTTTTTTCAAGCGAAAGTGTC1800.12862471595375227No Hit
GAGAATGGGGATGCTAAGAAGAAAATTACTGTTTTTCCAACTGTGTAAACTA1800.12862471595375227No Hit
GCATTGGGGATAGTTACTGAGACTTGACCAGGTAGTTGACAAAAAAAGTTTG1740.12433722542196052No Hit
GAAGTAGGGTGGGGTGTTCGACATTTTTCTTCCACAGCAGTTGGCCCATATT1730.12362264366666191No Hit
ACTACAGGGCCCACCTGCCCAGTTAGCCCGGCCAAGGACACTGATGGGTCCT1730.12362264366666191No Hit
GTACTGGGGAAAGACGAAAATTAGAATGACTCAATACAGGCAGTGGGAGTGC1720.12290806191136328No Hit
AAACACGGGACTATTATTACAATAAGCACTTACTAACATGATGGATATTATG1700.12147889840076603No Hit
TTGGCTGGGATATTAAGAGAATGGGTAGGAGTTCAGAGAAGGAGTTTAGAAT1680.12004973489016879No Hit
TGAAAAGGGAAGCTGGAATGTTTTACTTAGCCATGCAAGTCATTTATGTATA1670.11933515313487017No Hit
CCATAAGGGCATCATCCCTAAAGCTGCTTATGACCCATTTTTTCTATTCTAC1660.11862057137957153No Hit
TGGCCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1660.11862057137957153No Hit
TTCCGTGGGACAGCCTACTGGGAGGAGAAAAACAAGTAACATCTGAGGAAGG1640.11719140786897429No Hit
GTTCAGGGATAATACCTCATGACTAAGTGAGGTCCATCCAAAAAATACAAGG1630.11647682611367566No Hit
TACATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1620.11576224435837705No Hit
TGTGCAGGGGTTCACAGAACTAGAGAAACCTATTTTAAAAGTCATATGGAAC1610.11504766260307842No Hit
AGAATTGGGCAGAGGCACCAAGGAGACTTGGAAAACACTGTGGGCTGGAGCC1610.11504766260307842No Hit
TCACTCGGGGACGCTCTTTCGATCTCTAACAGGGTCAACAAGGGTTTTTTGT1580.11290391733718254No Hit
TTCTGTGGGCGAAGAAAGATTTGAATGGCTATGTAATTTTAACACTATCTAG1570.11218933558188392No Hit
TCTTTAGGGAGCCATGACCGTTATGAGGAAATATCCCCCATTCGAACTTAAC1570.11218933558188392No Hit
GTTGAGGGGGAAGTTGATGTTCTGCATTTTTATGAAACACTTTCTATTCTTT1560.11147475382658531No Hit
ACATTGGGGCTGTGTCCTCCTGGGAATTTCCCTTCAGGGACAGATGTGCTTA1560.11147475382658531No Hit
AACAACGGGTCTCGCACAGGGGCTGCAAAGTGTTTTCTGTGTCCCACTGTCT1550.11076017207128667No Hit
ACAACTGGGATGTACTGGCTGGTGGCAGGGCTTCTCTCCAGCAGAAAACAAC1540.11004559031598807No Hit
CTGCACGGGTCCTACTGAGCCTTTAGGACTGGAATCCAAGGAAGAAGGGATT1540.11004559031598807No Hit
GCAGGCGGGTCACCACCAAAATTGAGTAAGAATTATCACTTATAACATTGCC1540.11004559031598807No Hit
CACACCGGGTGAAATGTTTTAGTTCCAAATGCAGAAAGTGATTTTATTCCTC1540.11004559031598807No Hit
AATGCCGGGATTGCTCACTTCAGGGAGCAAAGAGGCCATATTAAGGAAGCCA1520.1086164268053908No Hit
ATTAGCGGGATGTTAAGTCATGGAATATCTTAATTGTTTAAGTGATTCTCAC1510.10790184505009219No Hit
GCATCCGGGGAAGGTTTGAGGGCCCTTGCAACCCCCTCCCAGCAGTGGCTGG1510.10790184505009219No Hit
ATTATTGGGATAAAATACAATTTTAATAACTACAATTGGGAAAACTCATCAA1500.10718726329479356No Hit
GCCCTGGGGAGGTCACTGGTTGAACCATGGGAAGGAACACTGCTTGGTGAGC1490.10647268153949492No Hit
TACTGTGGGGATTTACCATCTTACAAAATTAAATTTTGTTTCATAAACTAAA1490.10647268153949492No Hit
AGCGAGGGAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGCCAATGGCTT1490.10647268153949492No Hit
TATACAGGGATGGAAATTAAAATAATTATTTGAACTGAACAATAATAATGAC1470.10504351802889768No Hit
TTCAACGGGACAATTACACTGTTTTGCTTGTTGCCTTTTCTTGGTTTAACAA1470.10504351802889768No Hit
ATGAAGGGCAAGAGGATTGAGTACTTCTAAAATTAAATTTTTAATGAAAGTC1460.10432893627359906No Hit
TGTGCAGGGATAACTTCCTGAGGGAAGCTCTGGAAGTGGCAGTAGCTGGAAC1460.10432893627359906No Hit
GCGCATGGGCAAGTAAAATTTCCTGTAACAGCATTTCTCCTGTATCTGAATT1450.10361435451830045No Hit
GACCATTGGGCTGGAAGGAATGGCCGGAAAGGATGTTACCTGGGAAATACTC1450.10361435451830045No Hit
TGGCTTGGGATTTGCAGGCTTTTCTGAATACCAAATCTGCTTTTTGTAAAGC1440.10289977276300181No Hit
GTACTGGGGCTGTTACTCCGTAGGCTTCTTAATCTTCCAGGCATTACAGCAG1440.10289977276300181No Hit
TCAGTTGGGATCTCTGTGGTTTTTGTCCTGCGGAATTGTTCACAAATACACG1440.10289977276300181No Hit
TTGCCTGGGAAGGCCAGATGTGGTGGCTTACACCTATAATCCCAGCACTTTG1440.10289977276300181No Hit
ATTTCAGGGGTCTTAATTATTTAAAGTCCAGTTTTTTTCCTGCAGCAAACCT1440.10289977276300181No Hit
TCAATCGGGCACAGTGGCTACAGAGATTTCCAGCTGGGGAAGTAGTACCAAA1440.10289977276300181No Hit
GACCAAGGGAAAGCACTGGGTTTATTATGTATTAGTGCTGTAGTTATACTAA1440.10289977276300181No Hit
GACTAAGGGATGTATAACATCTTTTAATGTGATGTAAGTATGTAGGTATAAA1440.10289977276300181No Hit
TACAATGGGCATCTTTTCATGGAAAGAAAATGAGGATTCAATGTAATTTTCT1430.10218519100770321No Hit
ATAGCCGGGTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCA1430.10218519100770321No Hit
ACATCAGGGACCTGAGGAAGGTGAGTGAGTGCAGACAGATGGGGCCTGGTGC1430.10218519100770321No Hit
AAGCAGGGGCAAACTGACTAGTTCAACAAGCAGAATCATAGCCTAGTGGGCA1430.10218519100770321No Hit
GATAATGGGGTTGAGTAAGGCGAGGAAGGCTTCTCTGAGGAGGTGACCATCA1420.10147060925240457No Hit
GTTTCCGGGGGAATTCCGTAAACTATATTTTAAGCTTATTTCTTCAAAATTA1420.10147060925240457No Hit
GTGCTCGGGTGTTCTCTCAAGAAAGAGGGAATAATTATAAGGTCTACTGGAG1420.10147060925240457No Hit
TCTATAGGGAAAGAGATCCTGTTTGGCCACTTATAGGAGGTCCCTAAAGCTG1410.10075602749710594No Hit
GTTTGCGGGACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTCACCA1410.10075602749710594No Hit
ATCATTGGGCAATTCTTCAGTTTAGTCACTTGAGTGAAGCCCGAATGCTATA1400.10004144574180733No Hit
TATCAGGGACCTATGTCTCAATTGTGTATCATAGAGAAACGAACCCGAACAG1400.10004144574180733No Hit
TATAACGGGAATGAAGAAATTTTTAGTATTTTCTTGGAAAAGTGTAGATTCT1400.10004144574180733No Hit
CAGAGAAGGGGATTCCCTAGACACCGAATGCCTTGTTTGTATGAGTGAATAA1400.10004144574180733No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)