FastQCFastQC Report
Sat 31 Dec 2022
SRR5845038.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845038.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137877
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTCGCATATCTCGTATGC8560.6208432153296054No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTCGCATATCTCGTATGCCGTCT7460.5410619610232309No Hit
CGTACAGGGTAATTTGACATTCTAGAATTGTTAATTTTAGATAAAACCTCCT3500.25384944552028255No Hit
TCAGCGGGTGGGGAGAATGTGACATTATAGAAATAAGAGAAAATCAGAAATT2880.20888182945668965No Hit
TGAAATGGGGGCATCAAGGTAGACAAGGGCGTGGTCCCCCTGGCAGGGACAA2420.17551875947402396No Hit
GCTTGGGGGCGGCCACTTATTCGTGATGCCAACAATTTTGTATTAGATGAAC2360.17116705469367627No Hit
AGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG2300.16681534991332853No Hit
TAGCAAGGGCAAGTTTACCTTTTTGAAAGAAGTTAAACCAATAATTTATTTT2240.16246364513298084No Hit
TCAGAATGGGAGAAAATATTTGCTTAACACTAAACAAGGCAGTAAAGAAGAA2160.15666137209251726No Hit
GAGAATGGGGATGCTAAGAAGAAAATTACTGTTTTTCCAACTGTGTAAACTA2090.1515843831821116No Hit
CCGCTGGGATTACAGGCGTGAGCCACCGCACCTGGCCTGCCTGTTTTACCTT2050.1486832466618798No Hit
GAATCTGGGATATTGGGTTTTAAAAAAGTGTATTGTGACTTAAACTGTTATA2000.14505682601159003No Hit
TACACAGGGAGCACAGGCACACTTAATACATTTTTTTTCAAGCGAAAGTGTC1960.14215568949135823No Hit
ACAACTGGGATGTACTGGCTGGTGGCAGGGCTTCTCTCCAGCAGAAAACAAC1940.14070512123124235No Hit
AGATACGGGGAGGCGAAGATGGTGCACATACCCAGATATCTGAGTCAGGGAA1920.13925455297112643No Hit
TTGCTCGGGAATATTCTTACTCTTAGTATTAAATAATTTTATGAGTAAAAGA1840.13345227993066283No Hit
ACTACAGGGCCCACCTGCCCAGTTAGCCCGGCCAAGGACACTGATGGGTCCT1840.13345227993066283No Hit
TACATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1830.1327269958006049No Hit
AGCCTGGGTGACAGAGCAAGGTGCTATCTCCAAAATAAATAAATAAATGTTA1830.1327269958006049No Hit
GCATCCGGGGAAGGTTTGAGGGCCCTTGCAACCCCCTCCCAGCAGTGGCTGG1790.12982585928037307No Hit
CTGCACGGGTCCTACTGAGCCTTTAGGACTGGAATCCAAGGAAGAAGGGATT1760.12765000689019923No Hit
TGCAGCGAGTAATAAATGGTGAGGAAAAGAAAGAGGATGGATACAGCAACTG1750.12692472276014127No Hit
TGTGCAGGGGTTCACAGAACTAGAGAAACCTATTTTAAAAGTCATATGGAAC1750.12692472276014127No Hit
AACAACGGGTCTCGCACAGGGGCTGCAAAGTGTTTTCTGTGTCCCACTGTCT1720.12474887036996744No Hit
AGCGAGGGAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGCCAATGGCTT1720.12474887036996744No Hit
GTACTGGGGAAAGACGAAAATTAGAATGACTCAATACAGGCAGTGGGAGTGC1710.12402358623990947No Hit
TGGCCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1710.12402358623990947No Hit
GCGCATGGGCAAGTAAAATTTCCTGTAACAGCATTTCTCCTGTATCTGAATT1690.12257301797979359No Hit
TATAACGGGAATGAAGAAATTTTTAGTATTTTCTTGGAAAAGTGTAGATTCT1690.12257301797979359No Hit
TGAAAAGGGAAGCTGGAATGTTTTACTTAGCCATGCAAGTCATTTATGTATA1690.12257301797979359No Hit
GACCCAGGGATATTTGGTTAAATGTTTGATTGATAGAAAGTTTTTTATGGGT1680.12184773384973564No Hit
TCAATCGGGCACAGTGGCTACAGAGATTTCCAGCTGGGGAAGTAGTACCAAA1670.12112244971967769No Hit
TTTGTGGGGAGTAAGTTACAGTAACAAAATAACCTTTCATGTTTGGGATCTG1660.12039716558961973No Hit
GATAATGGGGTTGAGTAAGGCGAGGAAGGCTTCTCTGAGGAGGTGACCATCA1650.11967188145956179No Hit
TGTGCAGGGATAACTTCCTGAGGGAAGCTCTGGAAGTGGCAGTAGCTGGAAC1650.11967188145956179No Hit
CCATAAGGGCATCATCCCTAAAGCTGCTTATGACCCATTTTTTCTATTCTAC1640.11894659732950383No Hit
ATAAGGGGGGCTGCCAAGAATGTGGCCTTGCTGGCTTACAACCGCTGGTACC1620.11749602906938794No Hit
AAACGTGGGACCTCAAGATCTGTCCCTTGGTTTACATGCTATAATTTGTCTT1610.11677074493932997No Hit
GTTTCCGGGGGAATTCCGTAAACTATATTTTAAGCTTATTTCTTCAAAATTA1610.11677074493932997No Hit
ACATTGGGGCTGTGTCCTCCTGGGAATTTCCCTTCAGGGACAGATGTGCTTA1600.11604546080927203No Hit
GCATTGGGGATAGTTACTGAGACTTGACCAGGTAGTTGACAAAAAAAGTTTG1590.11532017667921408No Hit
GCAGGCGGGTCACCACCAAAATTGAGTAAGAATTATCACTTATAACATTGCC1590.11532017667921408No Hit
ATTTCAGGGGTCTTAATTATTTAAAGTCCAGTTTTTTTCCTGCAGCAAACCT1560.11314432428904023No Hit
ACATCAGGGACCTGAGGAAGGTGAGTGAGTGCAGACAGATGGGGCCTGGTGC1560.11314432428904023No Hit
ATCATTGGGCAATTCTTCAGTTTAGTCACTTGAGTGAAGCCCGAATGCTATA1550.11241904015898227No Hit
TATAGAGGGGGGGTTGTCATGGGCCAAATTAACATTTTGAGATTCTGTTAGG1550.11241904015898227No Hit
GTTTGCGGGACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTCACCA1550.11241904015898227No Hit
GATCACGGGTCCCAGTGGGATGACCCTCACTGAGAGTAGCAGCAAGGGAACC1540.11169375602892433No Hit
AAAAGAGGGAGCATTGATATGAACCATGGACAAGTATATTCTGCTGCCACAA1540.11169375602892433No Hit
ATTAGCGGGATGTTAAGTCATGGAATATCTTAATTGTTTAAGTGATTCTCAC1530.11096847189886638No Hit
AAACACGGGACTATTATTACAATAAGCACTTACTAACATGATGGATATTATG1530.11096847189886638No Hit
TGAACTGGGCTGCCATTATTGATTTTGATGCTGAAATGTGGCTTCTGTGTTC1520.11024318776880844No Hit
GTACTGGGGCTGTTACTCCGTAGGCTTCTTAATCTTCCAGGCATTACAGCAG1510.10951790363875047No Hit
TACTGTGGGGATTTACCATCTTACAAAATTAAATTTTGTTTCATAAACTAAA1500.10879261950869253No Hit
TCTTTAGGGAGCCATGACCGTTATGAGGAAATATCCCCCATTCGAACTTAAC1500.10879261950869253No Hit
CTTCTTGGGAATGTGCCTTGGAATTACCTCTGGTACTTGTTAAATTACATCA1480.10734205124857664No Hit
TCATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1480.10734205124857664No Hit
TGGCTTGGGATTTGCAGGCTTTTCTGAATACCAAATCTGCTTTTTGTAAAGC1470.10661676711851868No Hit
GAAATAGGGGAGGCCGTCATGGCGGTGCTCAGGGAGCTGCCGCGGCCCATGC1460.10589148298846073No Hit
TAAATAGGGGTTGTGATGAGCTGGGATCCTCCTGCTCAATCTCAGCTTGAAG1460.10589148298846073No Hit
GTTCAGGGATAATACCTCATGACTAAGTGAGGTCCATCCAAAAAATACAAGG1460.10589148298846073No Hit
TTTTAGGGGGGATAGTAAATTGCAGGGCCATCCTATCTATAAGGGACTCTAA1460.10589148298846073No Hit
TTCAACGGGACAATTACACTGTTTTGCTTGTTGCCTTTTCTTGGTTTAACAA1450.10516619885840278No Hit
TACAATGGGCATCTTTTCATGGAAAGAAAATGAGGATTCAATGTAATTTTCT1450.10516619885840278No Hit
GAAGTAGGGTGGGGTGTTCGACATTTTTCTTCCACAGCAGTTGGCCCATATT1450.10516619885840278No Hit
AATGCCGGGATTGCTCACTTCAGGGAGCAAAGAGGCCATATTAAGGAAGCCA1440.10444091472834482No Hit
GTTGGCGGGATAGTGGACTATTGTGTTCGTTTTGTGACAAGTAGAGAAGAGA1440.10444091472834482No Hit
AAGAACGGGGTGGTGAGGGAGATTAGAATTTCTGACTGAAGGAAGACTTTGG1440.10444091472834482No Hit
CAAAGGGGGTGTGTAGTTATATATTCATTTAAATTAATGATGGTTGTAATTC1430.10371563059828688No Hit
TTCCGTGGGACAGCCTACTGGGAGGAGAAAAACAAGTAACATCTGAGGAAGG1420.10299034646822894No Hit
AGAATTGGGCAGAGGCACCAAGGAGACTTGGAAAACACTGTGGGCTGGAGCC1420.10299034646822894No Hit
CACACCGGGTGAAATGTTTTAGTTCCAAATGCAGAAAGTGATTTTATTCCTC1420.10299034646822894No Hit
GCCCTGGGGAGGTCACTGGTTGAACCATGGGAAGGAACACTGCTTGGTGAGC1410.10226506233817097No Hit
TATTATGGGAAGGCATTGCTAATATCAATCAACACTGAAATTTTAAAAATGT1410.10226506233817097No Hit
GCACAAGGGCAGTCTTTAGGATTTGAACTCAAATCTTAATCCCCATAGCTAC1400.10153977820811302No Hit
TCAATTGGGAAACTAAATGTGGTCTGGTCCTACATAAAATATTTTAAAGGCA1400.10153977820811302No Hit
CTGCATGGGATTATCTATAATGTATAAAGAATACCTATAAGTCATTAAGAAA1390.10081449407805508No Hit
TCAGTAGGGTTAGGGTGGGGGATTGGTTTGGCTTTCTTTTGTTGTGGCTTTT1390.10081449407805508No Hit
GACACTGGAGAGTATTTTATTCCAATAGAATAAACCAGGAATCTCGGACTGT1390.10081449407805508No Hit
TCACTCGGGGACGCTCTTTCGATCTCTAACAGGGTCAACAAGGGTTTTTTGT1390.10081449407805508No Hit
TGACCTGGGGCAGACTATTTCAAGACCCTAAAAACATCCCTCATGGCCTCCC1390.10081449407805508No Hit
GTAGGCGGGTCTTCGGCAGGGATTTATTGAAAAGTGTATCTTTTCTTTATTG1380.10008920994799714No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)