FastQCFastQC Report
Sat 31 Dec 2022
SRR5845037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139073
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGTCGCATATCTCGTATGC8740.6284469307485997No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTCGCATATCTCGTATGCCGTCT7310.5256232338412201No Hit
CGTACAGGGTAATTTGACATTCTAGAATTGTTAATTTTAGATAAAACCTCCT3220.2315330797494841No Hit
TCAGCGGGTGGGGAGAATGTGACATTATAGAAATAAGAGAAAATCAGAAATT2800.20133311282563834No Hit
TAGCAAGGGCAAGTTTACCTTTTTGAAAGAAGTTAAACCAATAATTTATTTT2380.17113314590179257No Hit
GCTTGGGGGCGGCCACTTATTCGTGATGCCAACAATTTTGTATTAGATGAAC2110.15171888145074888No Hit
AGATACGGGGAGGCGAAGATGGTGCACATACCCAGATATCTGAGTCAGGGAA2090.15028078778770862No Hit
TGAAATGGGGGCATCAAGGTAGACAAGGGCGTGGTCCCCCTGGCAGGGACAA1990.14309031947250725No Hit
AGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG1930.13877603848338643No Hit
GAGAATGGGGATGCTAAGAAGAAAATTACTGTTTTTCCAACTGTGTAAACTA1930.13877603848338643No Hit
CCATAAGGGCATCATCCCTAAAGCTGCTTATGACCCATTTTTTCTATTCTAC1840.1323046169997052No Hit
TGGCCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1820.13086652333666493No Hit
AAACACGGGACTATTATTACAATAAGCACTTACTAACATGATGGATATTATG1790.1287093828421045No Hit
TACACAGGGAGCACAGGCACACTTAATACATTTTTTTTCAAGCGAAAGTGTC1780.12799033601058438No Hit
CCGCTGGGATTACAGGCGTGAGCCACCGCACCTGGCCTGCCTGTTTTACCTT1770.12727128917906422No Hit
GACCCAGGGATATTTGGTTAAATGTTTGATTGATAGAAAGTTTTTTATGGGT1730.12439510185298368No Hit
AATGCCGGGATTGCTCACTTCAGGGAGCAAAGAGGCCATATTAAGGAAGCCA1720.12367605502146355No Hit
GATAATGGGGTTGAGTAAGGCGAGGAAGGCTTCTCTGAGGAGGTGACCATCA1700.12223796135842327No Hit
ATAAGGGGGGCTGCCAAGAATGTGGCCTTGCTGGCTTACAACCGCTGGTACC1700.12223796135842327No Hit
GAATCTGGGATATTGGGTTTTAAAAAAGTGTATTGTGACTTAAACTGTTATA1700.12223796135842327No Hit
CGCCATGGGACAACTTTCAAAAAAAGTCCTCATGGAACCTGATAAATAAGAA1670.12008082086386286No Hit
AGCCTGGGTGACAGAGCAAGGTGCTATCTCCAAAATAAATAAATAAATGTTA1670.12008082086386286No Hit
TATAACGGGAATGAAGAAATTTTTAGTATTTTCTTGGAAAAGTGTAGATTCT1660.11936177403234273No Hit
TCAGAATGGGAGAAAATATTTGCTTAACACTAAACAAGGCAGTAAAGAAGAA1640.11792368036930247No Hit
AGCGAGGGAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGCCAATGGCTT1640.11792368036930247No Hit
AGAATTGGGCAGAGGCACCAAGGAGACTTGGAAAACACTGTGGGCTGGAGCC1630.11720463353778231No Hit
ACAACTGGGATGTACTGGCTGGTGGCAGGGCTTCTCTCCAGCAGAAAACAAC1610.11576653987474204No Hit
GCGCATGGGCAAGTAAAATTTCCTGTAACAGCATTTCTCCTGTATCTGAATT1600.1150474930432219No Hit
TACTGTGGGGATTTACCATCTTACAAAATTAAATTTTGTTTCATAAACTAAA1600.1150474930432219No Hit
GTTGAGGGGGAAGTTGATGTTCTGCATTTTTATGAAACACTTTCTATTCTTT1590.11432844621170177No Hit
AAGAACGGGGTGGTGAGGGAGATTAGAATTTCTGACTGAAGGAAGACTTTGG1590.11432844621170177No Hit
CTGCACGGGTCCTACTGAGCCTTTAGGACTGGAATCCAAGGAAGAAGGGATT1580.11360939938018164No Hit
TCATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1580.11360939938018164No Hit
TCAATCGGGCACAGTGGCTACAGAGATTTCCAGCTGGGGAAGTAGTACCAAA1580.11360939938018164No Hit
GCCCTGGGGAGGTCACTGGTTGAACCATGGGAAGGAACACTGCTTGGTGAGC1560.11217130571714136No Hit
TTCCGTGGGACAGCCTACTGGGAGGAGAAAAACAAGTAACATCTGAGGAAGG1560.11217130571714136No Hit
GTACTGGGGAAAGACGAAAATTAGAATGACTCAATACAGGCAGTGGGAGTGC1540.1107332120541011No Hit
TGGCTTGGGATTTGCAGGCTTTTCTGAATACCAAATCTGCTTTTTGTAAAGC1530.11001416522258094No Hit
TTGCTCGGGAATATTCTTACTCTTAGTATTAAATAATTTTATGAGTAAAAGA1520.1092951183910608No Hit
TCACTCGGGGACGCTCTTTCGATCTCTAACAGGGTCAACAAGGGTTTTTTGT1520.1092951183910608No Hit
GCGACTGGGATCTATTTCTGAGTTGAATACTCAGCTCCCACGAGAAAGAAAG1520.1092951183910608No Hit
TAAATAGGGGTTGTGATGAGCTGGGATCCTCCTGCTCAATCTCAGCTTGAAG1510.10857607155954067No Hit
GTTCAGGGATAATACCTCATGACTAAGTGAGGTCCATCCAAAAAATACAAGG1510.10857607155954067No Hit
GTTTCCGGGGGAATTCCGTAAACTATATTTTAAGCTTATTTCTTCAAAATTA1510.10857607155954067No Hit
ACTACAGGGCCCACCTGCCCAGTTAGCCCGGCCAAGGACACTGATGGGTCCT1510.10857607155954067No Hit
GTTTGCGGGACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTCACCA1490.1071379778965004No Hit
ATTATTGGGATAAAATACAATTTTAATAACTACAATTGGGAAAACTCATCAA1490.1071379778965004No Hit
TGCAGCGAGTAATAAATGGTGAGGAAAAGAAAGAGGATGGATACAGCAACTG1480.10641893106498027No Hit
TTTGTGGGGAGTAAGTTACAGTAACAAAATAACCTTTCATGTTTGGGATCTG1480.10641893106498027No Hit
TGAAAAGGGAAGCTGGAATGTTTTACTTAGCCATGCAAGTCATTTATGTATA1480.10641893106498027No Hit
CACACCGGGTGAAATGTTTTAGTTCCAAATGCAGAAAGTGATTTTATTCCTC1480.10641893106498027No Hit
ACCCAAGGGAAGAAGGGAATTCTTGCTTTCGAAGGCAGCAATTGAGAGATGA1470.10569988423346013No Hit
TTCCTAGGGAACAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGA1470.10569988423346013No Hit
GCAGGCGGGTCACCACCAAAATTGAGTAAGAATTATCACTTATAACATTGCC1470.10569988423346013No Hit
TACATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1460.10498083740193997No Hit
AAGCAGGGGCAAACTGACTAGTTCAACAAGCAGAATCATAGCCTAGTGGGCA1460.10498083740193997No Hit
GCATCCGGGGAAGGTTTGAGGGCCCTTGCAACCCCCTCCCAGCAGTGGCTGG1460.10498083740193997No Hit
TACAATGGGCATCTTTTCATGGAAAGAAAATGAGGATTCAATGTAATTTTCT1450.10426179057041986No Hit
TCTATAGGGAAAGAGATCCTGTTTGGCCACTTATAGGAGGTCCCTAAAGCTG1440.10354274373889973No Hit
GCATTGGGGATAGTTACTGAGACTTGACCAGGTAGTTGACAAAAAAAGTTTG1440.10354274373889973No Hit
ACATTGGGGCTGTGTCCTCCTGGGAATTTCCCTTCAGGGACAGATGTGCTTA1440.10354274373889973No Hit
TTCAACGGGACAATTACACTGTTTTGCTTGTTGCCTTTTCTTGGTTTAACAA1430.10282369690737957No Hit
GTACTGGGGCTGTTACTCCGTAGGCTTCTTAATCTTCCAGGCATTACAGCAG1430.10282369690737957No Hit
GACTAAGGGATGTATAACATCTTTTAATGTGATGTAAGTATGTAGGTATAAA1430.10282369690737957No Hit
GATCACGGGTCCCAGTGGGATGACCCTCACTGAGAGTAGCAGCAAGGGAACC1410.1013856032443393No Hit
TTAAAACGGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGT1410.1013856032443393No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)