FastQCFastQC Report
Sat 31 Dec 2022
SRR5845027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214365
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT8610.4016513889860751No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC7710.3596669232384018No Hit
TCAGCTGGGTCAGTAAACTATGGTACATTCCCACAATAAAATATAATGCAGT3280.15301005294707626No Hit
AAATTAGGGCAGTGGAAGCTTTGCAACAGTTGAATGATTTAAGATTATTATG3210.149744594500035No Hit
GATTACGGGGAGTGATTGGGAGAGAATAGGAAGAAACAACAAATGCAAATAT3170.14787861824458284No Hit
CACGCCGGGGCAGAGCTACATGGCTCCTTCATGTTACAAACTTCTAAAGTAA3090.14414666573367854No Hit
TGAAAGGGAATATTCAGATGCAACAAAATTACAGATTGTTAAATTTCACTGA3030.14134770135050032No Hit
TTGCCTGGGACAGTTTTGTGGTAACCGAATAGTTAGTGGTTCTGATGACAAC3000.1399482191589112No Hit
GCTACGGGGATGATTATTTGAAATACACTTGTGTTAGTAACTGCTTCTATAA2960.13808224290345905No Hit
AATTGTGGGATGGTTTATTTAGAATTTAAAATACCAGTAAACTAGATCTGAT2820.13155132600937652No Hit
ACATGCGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGCG2810.13108483194551349No Hit
GGTTCAGGGTAGTCTCAAATAGGTGCTTCCCCTGAGGTGCAGGGGAAGACCA2810.13108483194551349No Hit
AATACAGGGGAATACCATGATCGATGATGTCCCATGCTTCGAGGAAAACCAA2800.13061833788165045No Hit
TCCAATGGGCACCTCAATGAATTTTCACAAATCAAGCTCATCCATGTAACTA2800.13061833788165045No Hit
GGCCCAAAAAGTTATTAGACCCAATAAGATAATTCAGGAAGTCCAAGAGGTC2790.1301518438177874No Hit
GCAAATGGGGTCATTTGTACATTTTAACCTGAACATAGCAGTTACCTCTATG2730.12735287943460918No Hit
GTAGTTGGGCCTAAATAAATGAGCTGTTGGAAGAAAAGAATCACAGTACTTT2720.12688638537074615No Hit
AGTCAAGGGATTTTCAGATATGATTGTTTATGTAAACTAAAAAATTACTGCA2710.12641989130688314No Hit
TAACATGGGTGTGCTAAGATGAATAGAACACAAGGAAATAAAAATCTCATAG2680.125020409115294No Hit
GTAAATGGGGCAAGTTGTCATTCATTGAAAATGATTCTTACCTTTAGCTGAT2680.125020409115294No Hit
TTTTATGGGGAGCACAAAATAAATGGTGTTTTGGGTGAATATATAGTTTTGA2660.12408742098756795No Hit
TCTCCGGGGGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAAC2660.12408742098756795No Hit
TTCATCGGGAAAGAACTGAGGAAAAGAAGTGGTAAGCTAATGTTAAGGTGTC2650.1236209269237049No Hit
CTCGTTGGGGTATATAATAACTATGATCAAAAAGCTATTTGGAATAGTTTGA2640.12315443285984187No Hit
GGTATTGGGATGGAGTGTCCGAAATATGCAGATTCATGAACAGAAAGTAGAT2610.12175495066825273No Hit
TTCCCTGGGCCTACAGTTGGTATTACCTCTGTGCTCAGTGCCAGGCAAAACA2610.12175495066825273No Hit
TATGCTGGGGACCTTTTCTTCAGGATTACAGGTACACTGGATGCAGCCATGC2600.12128845660438971No Hit
ACATCAGGGTCCTTGCATTTAAAAGTTATTGATACTTACTGTCTTTTTAGAG2570.11988897441280061No Hit
TTATCTGGGAGTATGTATTAAGTCAAATTTTATAAAAAAGAAAGTTGGTCCT2560.11942248034893756No Hit
ACTACAGGCAAGATCTTCATAATAATGCAGTGTGTTCTACTGATTGCATTGT2560.11942248034893756No Hit
CACTCTGGGCGATCTTTGTGAGGGAATGATCATAAGTTGGACAATGATTACT2560.11942248034893756No Hit
TTAATGGGGAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGTAAAG2550.11895598628507452No Hit
AGTCATGGGCCATAAAACTGTCTGTATAACTCCCTGGGTTTACAAGCTCCTG2550.11895598628507452No Hit
ATTGATGGGGGCTTTTCATAATTGTCAAGTTCATTTAGTTCAGACGGATTCC2550.11895598628507452No Hit
TCACAAGGGGAATGCTCAATGTCAAATGGGACCGGATTGCCCCTGCTAGCAA2550.11895598628507452No Hit
GACGTTGGGTTTGAAAATAAATTACAGATATTAATATACTTCTCCCTGAGTA2540.11848949222121148No Hit
TCAGTAGGGTCTAGTGTTTTGCTTTCTTTTCCTTATAATAAATGACCCTTTT2540.11848949222121148No Hit
CGTTAAGGGCAGAGGAATAATTCACAGTGGAGATGGGGTAGATAGTTTTGTA2530.11802299815734844No Hit
TGTAACGGGATTCCTAAGGCAAATAAATGTACAAAAATAACTTACATTCACC2530.11802299815734844No Hit
CGTTCAGGGGAGTTGGGAAAACGGATCCTGTCACAAGAGGCATTGAGATCAC2530.11802299815734844No Hit
ATCTACGGGCATGGTGAATTAACTTTCTGATTTGCTGCTGGATTCAATGTGT2520.11755650409348542No Hit
ACTCCAGGGAGAGGCCAGTGGGTTTAAAAAGCTAAGTCCGAAATTTGTTAAA2520.11755650409348542No Hit
GCCACAGGGTTCTATGAAAAATGGCATTGATATTTTGATAGAAATAGCATTG2520.11755650409348542No Hit
CTTTGACAGGGAGTCCACTTGGCTGTACATAATAATGATCCATCCTGCTTTG2510.11709001002962237No Hit
TCGCTTGGGAACCCATGTGATAGCAGCCTCGAAAGAAGCATTTTATACCTGG2500.11662351596575934No Hit
TCGTCAGGGATGTTGGAAGATTTGGGAATGGATGATGAAGGAGATGATGACC2500.11662351596575934No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC2500.11662351596575934No Hit
TCAATTGGGACGGGGGGTCAGGCCTGTTGCATTTTACATTGCCAGACTTTTT2490.11615702190189629No Hit
TCACCACGGGGATAATCATAAGGTGTTTGTTAGGAGCGCTAGACCACCTGGA2490.11615702190189629No Hit
ACGGCTGGGTCCTTCATTTCTGTCATTCAAGCTCAACGGCTATTGTGGTGAC2490.11615702190189629No Hit
GGAACGGGAGTGAGTACAGTGTTGAAAATGAGAACTGGACTAAAGGGTCCTT2490.11615702190189629No Hit
GATGTCGGGAACAAAATAGAGTTTTAATTCCTGATTAGAGAGCCATAACTGT2480.11569052783803327No Hit
AGCCAAGGGAGAGGGGAGGCGCAGCCGGGCGGAGGGCCCACGAGGGCTCAGC2480.11569052783803327No Hit
GGCGTGGGCCTACACACAAGCGTTAAACGGTTTCTCTGGCATGCTCATTTTC2480.11569052783803327No Hit
ATAATCGGGCAAAAAGATGTGGAAGAAAGGCATATTACCTTTGGTTGACATT2470.11522403377417022No Hit
GCCAGAGGGATATCTGTGGACTGAATGCAATCTACGGGCCATCAATTCGTGG2470.11522403377417022No Hit
AGTTATGGGAGGGAGGAAATTTTGGAGGCAGAAGCTCTGGCCCCTATGGCGG2470.11522403377417022No Hit
GCACCTGGGACTACAATTTGAAGTCAGAAATGCCACTTGGGTAGCTAATGAA2460.11475753971030718No Hit
ATTATGGGGATCCCTTATGTGAAATTTTGTTTTTCCTAAGTTGAAAAATGAT2460.11475753971030718No Hit
GCATAAGGGCAATAGAACAGTGGCTATGGTCAATATTCCTAGGAATGGGAAC2450.11429104564644416No Hit
TAAAGCGGGAGACACAGGTCTTGCTCACAGGGTTCTTGCCATAAAGTAGACA2450.11429104564644416No Hit
ATTGATGGGAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCA2450.11429104564644416No Hit
TGTTAGGGGGTCGCTATTAGTTGGGAGTTGCAATACATTGTTGGCCATAAAC2440.1138245515825811No Hit
CTACTGACGGGATTCTTCAGATAAATAGTGGTGAAACTAGACTGAGTGATTT2440.1138245515825811No Hit
CAGCCTGGGGGATCTATAACGGGGATGGCCCACCATAATTACCCCCATACTC2440.1138245515825811No Hit
TACGGCGGGCATATACAGCCTTATATCTGTTACACTTAATTTGTAATTAAAA2430.11335805751871808No Hit
CAAGAAGGGCAACAGAAATAACAAAGTAGCACAGACTGTGGCTTAAACAACA2430.11335805751871808No Hit
ATGCTAGGGACACTTAGATGTTACTTAATCTTTCTGAGGTCTACTTTCTTCA2420.11289156345485503No Hit
ACAAAAGGGTCTCTATGGATTACCCAGACAAGACACTACATCCATCCAACAA2420.11289156345485503No Hit
CTGAGTGGGGGGGGACAAAGAGTTTTTATACACTTCTAAATTGCTTGCATTT2420.11289156345485503No Hit
GCATGCCGGGGAGGTCAAACTGGCATTTTTCCAGCCAACTACGTAACCATGA2390.11149208126326593No Hit
AATAATGGGTAAGCCTTTTCGATGTGCTGCTGGATTTGGTTTGGCAGTATTT2380.11102558719940289No Hit
AGACGCGGGATATGCAGTACAATATTATGAAAAATATTTTAAAAGGACAAAG2380.11102558719940289No Hit
ATCAGAGGGAAGGTGAATTCGTGATTATAAAATCTAGGCAAAACAACTCTGT2370.11055909313553984No Hit
GCATGTGGGCTGGACAGAAGGAATGTGTCAAACCGTGGTCTGCCAAGAAATA2370.11055909313553984No Hit
GCCAATGGGAGTCTAAAAAACCAGATGAAATTGAAGGTTGGGAGCCTCCAAA2370.11055909313553984No Hit
CTCCCAGGGCAGAAGCCTTCCATCTGGAGGATTAGGTGACCTTAAGGTCCCT2360.11009259907167682No Hit
CAGGTTGGGATATTTGACTTATTTTCATATTTATTGTCTACTTCCTTATGAA2360.11009259907167682No Hit
TGTAGCGGGATGTTTCAAAGGGAAAAATGTAGTTGTCTTCTGTACCTGTCAC2350.10962610500781376No Hit
TGGCTGGGGGATCTACGATTGGGGAGAGTGCATTCCCCACCCCCATGTTACT2350.10962610500781376No Hit
TACTCCGGGATGTAAAGCCTTTGTATGAAAGGTGGTTGGAGAAAATGATATT2340.10915961094395074No Hit
CAGCGAGGGGAAATTGTAAAAAAGAATATGAATATTTTACCTTTATAACTAC2340.10915961094395074No Hit
CAGCCCGGGAGATAAGATGAATGGCTTTCTAGAAGGTGTTGCTTTTTGTTTT2330.1086931168800877No Hit
TTAATGGGTGATTTGCAAAATCACAAAACTAAGTTGTCAGTTATCACTGTAG2330.1086931168800877No Hit
GACAAGGGGTAATTGTGAATATTAACTGAGACAGTGAAACATGGTGGCCGGG2330.1086931168800877No Hit
AATTACGGGGATCTTACAAAGGGCACATATAATCAGGGAGACAGGATAGACA2320.10822662281622467No Hit
GGAAATGGGCATTTTGGTTTTGTAAAAGCTGTTACTGAACTCAAGAAAATAT2320.10822662281622467No Hit
AGAACAGGGAACTGGTAAAGAATTGGTTCTCTGTAAATACCTGCTGCTTGAA2310.10776012875236163No Hit
TGCAAGGGGATCATTCATTCATTCTTTTAGCAAACCAAAGACAGTGTATATA2300.10729363468849858No Hit
GAACTAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC2300.10729363468849858No Hit
CAAGAAGGGAAGTATCTCAGAGCTCATAGAAGTTGACAGTTGATTAAAGTAC2300.10729363468849858No Hit
CCAATCGGGTGTTATGGAGATGGCTTTTTTCCTTAAACCTCATGAACCAAGC2300.10729363468849858No Hit
TACATAGGGGATGATCTCAAATGCTCCTCTCAGTGCTAAGATGTTTGATGTT2300.10729363468849858No Hit
CACACTGGGATCACATAATGGATTTTCTCCCCAAGTATGTTGCCTATCACTA2290.10682714062463555No Hit
ACGCAAGGGAGCCCGAGTAGGGGCTGGTGACAGTCAATTGAAAGGAGTCTCC2280.1063606465607725No Hit
AGATATGGGATAGCGATGAATCACCTGAAGCTGGTAAAATGCTTGAAGATGA2280.1063606465607725No Hit
CGGTGCGGGCATGAATTATTGAAAAATAGTCTATGTTCCAAGTCCATGATAT2270.10589415249690948No Hit
ATGCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2270.10589415249690948No Hit
AAATATGGGATTCTATAATTGGTAGCATATCATAATTTAATTGGCAACCAGT2270.10589415249690948No Hit
CAACAGGGGAGATGCTTTAAAAATATTCCCTTTTGTATAGTGAAACAACTTT2270.10589415249690948No Hit
TTTCGCGGGTCTTTCTTCCTGGAGAAGAGGAGATTTTCTCTTTTGAGAGTGG2270.10589415249690948No Hit
GGCGGTGGGGATCCCTGAAAACGCCAACGTCTTCTATGCCATGAACTCTACC2260.10542765843304645No Hit
TGCAAAGGGAAGTTTGGATCTTTTAAAATCAAATACACATATAAAAGCAATT2260.10542765843304645No Hit
CTAAGTGGGGATGAGAACCGTGCATTCAAGACAGATAACACACGTTTGGAAG2260.10542765843304645No Hit
AAACCAGGGCAGGACACGGTGGGTGTCAGGCCTGGTCAGGCAAGGGGGCTTT2250.1049611643691834No Hit
CTACTGGGGAACAGATGGAAGAAGAAAAGCAGCAAACAGAAAGGGTTACAAA2250.1049611643691834No Hit
TTAGATGGGGTCATTAGTAGTGAGCAAAAAAAGCAGGTTGCAGAAGGATACA2240.10449467030532036No Hit
AGTACTGGGGTGGGCAGTTTCAGAAAAGAAAACAAACAAACAAAAATCGACC2240.10449467030532036No Hit
TTGGATGGGGGTTCATATAAATGTACAAATAGCAGTTAGTATCAGTATAGGT2240.10449467030532036No Hit
CATGCTGGGATATGTTCTGTCATAGAAATTGTTTTCAAGCCAGTTTCCTTTT2240.10449467030532036No Hit
TGTTATGGGAAAATGATCTGGATTTCTATAGCAAGGCCCTTGGGGAGGGCAC2240.10449467030532036No Hit
TGCTATGGGGTTGGTAGGGTGATACTTTATAGAAATAAAGTAAATTGTGTAA2220.10356168217759429No Hit
ATTCTAGGGGACCGGGAGTGGGCGGGCCATTTCTTGTTCTCTCTCCCGCTCT2220.10356168217759429No Hit
ATTACTGGGATTCTGTTCTTTTATTTGCAGCTGGAAGCAGAAAAAAGTTTTG2210.10309518811373124No Hit
CGCTTTGGGATAAGTGGTATTTTTGAGAGATTTATTCTCTCTCCTCTGAAGC2210.10309518811373124No Hit
GTGGCGGGGATACAAAGCTATGTAATGTTACCTCTTGACTCGCTTTTGAAAG2200.10262869404986821No Hit
CTGGATGGGTGGCATTTATACTTTTTATTTCATTATTAAGAAAAATTTTGTC2200.10262869404986821No Hit
GCGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2200.10262869404986821No Hit
CATACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2200.10262869404986821No Hit
CCACCCGGGAGGTGTGTTATAGTTTTCTTCTGAAAGATAGTTTTTCTGTCTC2200.10262869404986821No Hit
ACAATGTGGGGAAATGGAAATGCACATGGTCAACATTCAGCACAAAATTGAG2190.10216219998600519No Hit
ATACTCGGGTAATTACATATTTATTTACCTGATAAATATGTATCTAGTTCTA2190.10216219998600519No Hit
AACTAAGGGGCTTTCCAGGGCAACCGTACTTTCCTAAACTTCATTTGTCCCA2180.10169570592214214No Hit
GCAAAAGGGTATTTCCTCTCATATATGCTTAGGTTTAAGTCAAATAGCCTGA2180.10169570592214214No Hit
ATGCAAGGGGATCTATTAGCGGGTTATGTACATGAGTAATTCTTTAGTGGAT2170.10122921185827911No Hit
CGTGCGGGGTGAAAATAGTTACTAAGCAACAGAAACTGATCTCAAATGACAG2170.10122921185827911No Hit
TGCAAGGGAGCTGAGACCTGAAGAATAAGGGACAATTTTACCCTGACTGCTG2170.10122921185827911No Hit
TTGAACGGGATCCTTGAAAGACTTAATAGAGATACATGAGACTAAGTTAATC2170.10122921185827911No Hit
GTGTACGGGGATTCTGCAATTCGAAAGCAGCTTGTTAAAAATGAGAAGGGCA2170.10122921185827911No Hit
ACTGATGGGGGAGATAAGAAGAAAGGCAAGGGGGTAAAAGGAGAAACGGAAT2160.10076271779441606No Hit
TGCCATAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG2160.10076271779441606No Hit
GTACTAGGGAGCTAGACAACAGTCCATGTTAATGGGAGACATTATCAACTGG2160.10076271779441606No Hit
CTAGTAGCGGGTATCTACAAATGCAAGTAAAATTTCCTGTAACAGCATTTCT2150.10029622373055302No Hit
AATAACGGGGTACATGCTGATGGTAGCCTTTGTGAAATTTTCCTAATTGGGC2150.10029622373055302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)