FastQCFastQC Report
Sat 31 Dec 2022
SRR5845026.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845026.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213073
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC8350.3918844715191507No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT8230.38625259887456415No Hit
TGAAAGGGAATATTCAGATGCAACAAAATTACAGATTGTTAAATTTCACTGA3340.1567537886076603No Hit
GATTACGGGGAGTGATTGGGAGAGAATAGGAAGAAACAACAAATGCAAATAT3290.1544071750057492No Hit
ACATGCGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGCG3200.15018327052230926No Hit
AAATTAGGGCAGTGGAAGCTTTGCAACAGTTGAATGATTTAAGATTATTATG3110.1459593660388693No Hit
CACGCCGGGGCAGAGCTACATGGCTCCTTCATGTTACAAACTTCTAAAGTAA3100.14549004331848708No Hit
AGTTATGGGAGGGAGGAAATTTTGGAGGCAGAAGCTCTGGCCCCTATGGCGG3070.14408207515734045No Hit
TGTAACGGGATTCCTAAGGCAAATAAATGTACAAAAATAACTTACATTCACC2920.1370422343516072No Hit
TAACATGGGTGTGCTAAGATGAATAGAACACAAGGAAATAAAAATCTCATAG2880.13516494347007832No Hit
GGCCCAAAAAGTTATTAGACCCAATAAGATAATTCAGGAAGTCCAAGAGGTC2880.13516494347007832No Hit
TCAGCTGGGTCAGTAAACTATGGTACATTCCCACAATAAAATATAATGCAGT2860.13422629802931388No Hit
TCCAATGGGCACCTCAATGAATTTTCACAAATCAAGCTCATCCATGTAACTA2810.13187968442740283No Hit
GCATAAGGGCAATAGAACAGTGGCTATGGTCAATATTCCTAGGAATGGGAAC2800.13141036170702058No Hit
TTGCCTGGGACAGTTTTGTGGTAACCGAATAGTTAGTGGTTCTGATGACAAC2790.13094103898663836No Hit
TCTCCGGGGGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAAC2760.12953307082549173No Hit
TTTTATGGGGAGCACAAAATAAATGGTGTTTTGGGTGAATATATAGTTTTGA2740.12859442538472732No Hit
TCGCTTGGGAACCCATGTGATAGCAGCCTCGAAAGAAGCATTTTATACCTGG2740.12859442538472732No Hit
GTAGTTGGGCCTAAATAAATGAGCTGTTGGAAGAAAAGAATCACAGTACTTT2730.1281251026643451No Hit
GGTTCAGGGTAGTCTCAAATAGGTGCTTCCCCTGAGGTGCAGGGGAAGACCA2700.12671713450319844No Hit
AGTCCTGGGCCTCCGGTCCCGTAGTCCAAATCAAGTTATTAAATTTAAACCT2680.125778489062434No Hit
CAGCCCGGGAGATAAGATGAATGGCTTTCTAGAAGGTGTTGCTTTTTGTTTT2670.12530916634205178No Hit
AGTCAAGGGATTTTCAGATATGATTGTTTATGTAAACTAAAAAATTACTGCA2660.12483984362166957No Hit
GGAAATGGGCATTTTGGTTTTGTAAAAGCTGTTACTGAACTCAAGAAAATAT2650.12437052090128735No Hit
TGTTAGGGGGTCGCTATTAGTTGGGAGTTGCAATACATTGTTGGCCATAAAC2650.12437052090128735No Hit
TGGCTGGGGGATCTACGATTGGGGAGAGTGCATTCCCCACCCCCATGTTACT2630.12343187546052291No Hit
GCACCTGGGACTACAATTTGAAGTCAGAAATGCCACTTGGGTAGCTAATGAA2620.1229625527401407No Hit
ACTACAGGCAAGATCTTCATAATAATGCAGTGTGTTCTACTGATTGCATTGT2610.12249323001975848No Hit
TCACCACGGGGATAATCATAAGGTGTTTGTTAGGAGCGCTAGACCACCTGGA2600.12202390729937626No Hit
AATACAGGGGAATACCATGATCGATGATGTCCCATGCTTCGAGGAAAACCAA2600.12202390729937626No Hit
GCCTTCGGGGCGCTCGGTGACCTTTCCGAGTTGGCTGCAGATTTGTGGTGCG2590.12155458457899407No Hit
CGTTAAGGGCAGAGGAATAATTCACAGTGGAGATGGGGTAGATAGTTTTGTA2580.12108526185861185No Hit
GTTACTGGGGTAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTA2580.12108526185861185No Hit
CTACTGACGGGATTCTTCAGATAAATAGTGGTGAAACTAGACTGAGTGATTT2580.12108526185861185No Hit
GTAAATGGGGCAAGTTGTCATTCATTGAAAATGATTCTTACCTTTAGCTGAT2570.12061593913822961No Hit
CACACTGGGATCACATAATGGATTTTCTCCCCAAGTATGTTGCCTATCACTA2570.12061593913822961No Hit
CGTTCAGGGGAGTTGGGAAAACGGATCCTGTCACAAGAGGCATTGAGATCAC2550.1196772936974652No Hit
TACTCCGGGATGTAAAGCCTTTGTATGAAAGGTGGTTGGAGAAAATGATATT2510.11780000281593633No Hit
TATAATGGGGATTATTAAAAAGTCAAGAAACAATAGATACTGGGAAGCTTGG2510.11780000281593633No Hit
GCCACAGGGTTCTATGAAAAATGGCATTGATATTTTGATAGAAATAGCATTG2510.11780000281593633No Hit
AAACCAGGGCAGGACACGGTGGGTGTCAGGCCTGGTCAGGCAAGGGGGCTTT2500.11733068009555411No Hit
CAGCCTGGGGGATCTATAACGGGGATGGCCCACCATAATTACCCCCATACTC2490.11686135737517189No Hit
GGAACGGGAGTGAGTACAGTGTTGAAAATGAGAACTGGACTAAAGGGTCCTT2490.11686135737517189No Hit
CTCGTTGGGGTATATAATAACTATGATCAAAAAGCTATTTGGAATAGTTTGA2480.11639203465478966No Hit
GCCAGAGGGATATCTGTGGACTGAATGCAATCTACGGGCCATCAATTCGTGG2470.11592271193440747No Hit
ATTGATGGGAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCA2460.11545338921402525No Hit
TCGTCAGGGATGTTGGAAGATTTGGGAATGGATGATGAAGGAGATGATGACC2450.11498406649364303No Hit
ACGGCTGGGTCCTTCATTTCTGTCATTCAAGCTCAACGGCTATTGTGGTGAC2450.11498406649364303No Hit
TAACGCGGGGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTT2440.11451474377326079No Hit
ACAAAAGGGTCTCTATGGATTACCCAGACAAGACACTACATCCATCCAACAA2440.11451474377326079No Hit
ACTCCAGGGAGAGGCCAGTGGGTTTAAAAAGCTAAGTCCGAAATTTGTTAAA2440.11451474377326079No Hit
CCAATCGGGTGTTATGGAGATGGCTTTTTTCCTTAAACCTCATGAACCAAGC2430.1140454210528786No Hit
TCAATTGGGACGGGGGGTCAGGCCTGTTGCATTTTACATTGCCAGACTTTTT2430.1140454210528786No Hit
TAGCTTGGGATTTCGGCCAAGTCATTCTTTATAAGCAGAGGCAAATATCATG2430.1140454210528786No Hit
TGCAAGGGGATCATTCATTCATTCTTTTAGCAAACCAAAGACAGTGTATATA2420.11357609833249638No Hit
AAAATCGGGAGTGAAACATTTTTTAAAAGGAAAAATAATAGCCCTTCAAGGT2420.11357609833249638No Hit
AGTCATGGGCCATAAAACTGTCTGTATAACTCCCTGGGTTTACAAGCTCCTG2420.11357609833249638No Hit
TATGCTGGGGACCTTTTCTTCAGGATTACAGGTACACTGGATGCAGCCATGC2420.11357609833249638No Hit
ATTGGCGGGAGAGGCGGACAAGATGGCGGCGGCAGCTGTACAGGGCGGGAGA2420.11357609833249638No Hit
GTTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2410.11310677561211416No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC2410.11310677561211416No Hit
ACATCAGGGTCCTTGCATTTAAAAGTTATTGATACTTACTGTCTTTTTAGAG2410.11310677561211416No Hit
ACAGAAGGGATTAGGGGCAAGATCCAGTCTGAGAGAAGTCTCCTTTGAGAAG2400.11263745289173194No Hit
TTGGATGGGGGTTCATATAAATGTACAAATAGCAGTTAGTATCAGTATAGGT2390.11216813017134973No Hit
AATTGTGGGATGGTTTATTTAGAATTTAAAATACCAGTAAACTAGATCTGAT2390.11216813017134973No Hit
GCTACGGGGATGATTATTTGAAATACACTTGTGTTAGTAACTGCTTCTATAA2380.11169880745096751No Hit
TTCCCTGGGCCTACAGTTGGTATTACCTCTGTGCTCAGTGCCAGGCAAAACA2380.11169880745096751No Hit
ACGCAAGGGAGCCCGAGTAGGGGCTGGTGACAGTCAATTGAAAGGAGTCTCC2370.11122948473058529No Hit
ATTCTAGGGGACCGGGAGTGGGCGGGCCATTTCTTGTTCTCTCTCCCGCTCT2370.11122948473058529No Hit
TTATCTGGGAGTATGTATTAAGTCAAATTTTATAAAAAAGAAAGTTGGTCCT2360.11076016201020308No Hit
TTAATGGGGAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGTAAAG2360.11076016201020308No Hit
ATAATCGGGCAAAAAGATGTGGAAGAAAGGCATATTACCTTTGGTTGACATT2360.11076016201020308No Hit
TCACAAGGGGAATGCTCAATGTCAAATGGGACCGGATTGCCCCTGCTAGCAA2360.11076016201020308No Hit
GTCTAAGGGATAACAAAAAATAGAAAATGTTGATAGTATGATAGATAGTGAA2360.11076016201020308No Hit
TACGGCGGGCATATACAGCCTTATATCTGTTACACTTAATTTGTAATTAAAA2350.11029083928982086No Hit
GATGTCGGGAACAAAATAGAGTTTTAATTCCTGATTAGAGAGCCATAACTGT2350.11029083928982086No Hit
TCAGTAGGGTCTAGTGTTTTGCTTTCTTTTCCTTATAATAAATGACCCTTTT2340.10982151656943864No Hit
CTCCAAGGGGTTGGAGTTCAGTAGAGCATTATAGGTTTTACACAATACCATT2340.10982151656943864No Hit
GATAAAGGGATTCATGTGATGGTAGGATTTGTCCTTTTTACATTCAGGTAGC2330.10935219384905642No Hit
TGTAGCGGGATGTTTCAAAGGGAAAAATGTAGTTGTCTTCTGTACCTGTCAC2330.10935219384905642No Hit
CAGTAGCGGGTAAAATATGTGCTTTCCAACCAGCACAGCTGGCGCTCTTAGC2330.10935219384905642No Hit
TTGAACGGGATCCTTGAAAGACTTAATAGAGATACATGAGACTAAGTTAATC2330.10935219384905642No Hit
AGAACAGGGAACTGGTAAAGAATTGGTTCTCTGTAAATACCTGCTGCTTGAA2320.10888287112867422No Hit
TCGAATGGGAAGACAGGGATGCCTACTTTCACCACTTCTATTCAGTATAGTA2320.10888287112867422No Hit
TTCATCGGGAAAGAACTGAGGAAAAGAAGTGGTAAGCTAATGTTAAGGTGTC2310.108413548408292No Hit
GTCATAGGGACAAGGTGCTGTCGGGAATCAAATAAAATATGTTATCAGAGAT2310.108413548408292No Hit
TGCTATGGGGTTGGTAGGGTGATACTTTATAGAAATAAAGTAAATTGTGTAA2310.108413548408292No Hit
GTGTACGGGGATTCTGCAATTCGAAAGCAGCTTGTTAAAAATGAGAAGGGCA2310.108413548408292No Hit
TGCAAAGGGAAGTTTGGATCTTTTAAAATCAAATACACATATAAAAGCAATT2300.10794422568790978No Hit
GCGCGGGGAGTAATTGGCAAAGTAAGTAAGAGAGAAAGCTGAAACTTAAACA2300.10794422568790978No Hit
AGCCAAGGGAGAGGGGAGGCGCAGCCGGGCGGAGGGCCCACGAGGGCTCAGC2300.10794422568790978No Hit
CGGTGCGGGCATGAATTATTGAAAAATAGTCTATGTTCCAAGTCCATGATAT2290.10747490296752756No Hit
GACACGGGGCCCGCTTGCACCTCGGCGATCCCCGACTCCCTTCTTTATGGCG2290.10747490296752756No Hit
GTTTGAGGGGCAAGGTGTAAATAATATTTTATTTCAAGGATATTTTTGAGGT2290.10747490296752756No Hit
GTGGCGGGGATACAAAGCTATGTAATGTTACCTCTTGACTCGCTTTTGAAAG2280.10700558024714535No Hit
GCAAATGGGGTCATTTGTACATTTTAACCTGAACATAGCAGTTACCTCTATG2280.10700558024714535No Hit
ATGAGGGCTCACACGGGGGGAGTCGATGGATGCTTGAGCTCCACATTGGAGC2280.10700558024714535No Hit
TACATAGGGGATGATCTCAAATGCTCCTCTCAGTGCTAAGATGTTTGATGTT2270.10653625752676313No Hit
CTAGTAGCGGGTATCTACAAATGCAAGTAAAATTTCCTGTAACAGCATTTCT2270.10653625752676313No Hit
ATTATGGGGATCCCTTATGTGAAATTTTGTTTTTCCTAAGTTGAAAAATGAT2260.10606693480638091No Hit
GTATGAAGGGAGCCACTCTATGAGTTGGACTTCAGTCTTGCCTAGGCGATTT2260.10606693480638091No Hit
GGTATTGGGATGGAGTGTCCGAAATATGCAGATTCATGAACAGAAAGTAGAT2260.10606693480638091No Hit
ACTTCGGGGTGCTATTTCAATTTGAAAATAGGTTCAGGAACTATCACTGTAT2250.10559761208599869No Hit
GACGTTGGGTTTGAAAATAAATTACAGATATTAATATACTTCTCCCTGAGTA2240.10512828936561648No Hit
ATGCTAGGGACACTTAGATGTTACTTAATCTTTCTGAGGTCTACTTTCTTCA2240.10512828936561648No Hit
GCCTCTGGGTTAAGAATGCTTTCCCAAATTCCATTATTTGACATTGAGTCTG2240.10512828936561648No Hit
AGATATGGGATAGCGATGAATCACCTGAAGCTGGTAAAATGCTTGAAGATGA2240.10512828936561648No Hit
AGCGCAGGGCATCCTGCAACAGCAGTCATAGGAGAATATGCCTCAATCAAAA2240.10512828936561648No Hit
TTGGCTGGGGCTGATTTGGATTTCAGGGTTGTGCCACTGATAAATAATTTCA2230.10465896664523426No Hit
GCATGCCGGGGAGGTCAAACTGGCATTTTTCCAGCCAACTACGTAACCATGA2220.10418964392485204No Hit
AAGCAAGGGGGAAGAAGTAGACTAATCTCTGGCTGAGGGATGACTTACCTGT2220.10418964392485204No Hit
GCGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2220.10418964392485204No Hit
CATCCTGGGGAGTGAGACTGACTGCGTCGGGGTTGAGACTGGGTGGATGAGG2220.10418964392485204No Hit
CAGCGAGGGGAAATTGTAAAAAAGAATATGAATATTTTACCTTTATAACTAC2220.10418964392485204No Hit
ACGCATGGGGAAGAGTACCTTTATGTCAGTTAATAAAAATAAATAATCTTAA2220.10418964392485204No Hit
GCCAATGGGAGTCTAAAAAACCAGATGAAATTGAAGGTTGGGAGCCTCCAAA2220.10418964392485204No Hit
TTCCACGGGATTCTTTTAATTCTTGCTATTTTGAGTTAAAGAGATTTATTGA2210.10372032120446982No Hit
ACAGCTGGGCCTGGCCTTGAATCAGCTGCCTCAAGTCTTTTCCCGACTCTTT2210.10372032120446982No Hit
CGAACTGGGAAGACAGCTTTACTTCTAATACAGAATGGTTGAATTTCCATTA2210.10372032120446982No Hit
TACACCGGGACAGAGCAAACTCTGCATGAACAAGGAAGGAAGGAATACATTC2210.10372032120446982No Hit
CGTGCTGGGTTCCTGAGTTACTTCATATAGAATAATGGCCTCCAGCTCCATC2200.10325099848408761No Hit
GAAAGCCTGCCCCTGGATGAGATCCTTATCCTGGCTGTGATGGCAGTGGCAG2200.10325099848408761No Hit
ATCAGAGGGAAGGTGAATTCGTGATTATAAAATCTAGGCAAAACAACTCTGT2200.10325099848408761No Hit
CATTCAGGGTACAAAATACCCAATAAATGTTTGGAAAAAGTTACATGCATGA2200.10325099848408761No Hit
TTAATGGGTGATTTGCAAAATCACAAAACTAAGTTGTCAGTTATCACTGTAG2200.10325099848408761No Hit
CTCCCAGGGCAGAAGCCTTCCATCTGGAGGATTAGGTGACCTTAAGGTCCCT2200.10325099848408761No Hit
AGAATTGGGATCATACTGGATTTTTTTCAGCTTGTTGCATACAATGAAAGGT2200.10325099848408761No Hit
ACACGTGGGCACTTTTACACTGTTGGTGGGAGTGTAAACTAGTTCAACCATT2190.1027816757637054No Hit
ATACTCGGGTAATTACATATTTATTTACCTGATAAATATGTATCTAGTTCTA2190.1027816757637054No Hit
AGCTTAGGGATGGAACTGATGGAAAAGAAACTTATGGATTACATTGATCAGC2180.10231235304332317No Hit
ATCTACGGGCATGGTGAATTAACTTTCTGATTTGCTGCTGGATTCAATGTGT2170.10184303032294095No Hit
CATACTGGGATCCCTACCCCCTTACTTGAGCCACATCACAGTTTCCTGAACT2170.10184303032294095No Hit
AATTACGGGGATCTTACAAAGGGCACATATAATCAGGGAGACAGGATAGACA2170.10184303032294095No Hit
TATTATGGGGGACTTCTGCATCCAGATTTTAGAATCATTTTTTAAATGCCTT2160.10137370760255876No Hit
GGCGGTGGGGATCCCTGAAAACGCCAACGTCTTCTATGCCATGAACTCTACC2160.10137370760255876No Hit
CGCTTTGGGATAAGTGGTATTTTTGAGAGATTTATTCTCTCTCCTCTGAAGC2160.10137370760255876No Hit
TCAAGTGGGATTTTGGTTTGTGGAGGAACTCAAGTCATTAATGAGGTGTCAG2160.10137370760255876No Hit
GTTTGCGGGGTACAGGCTGAGAGGCTGAGAAATCATCCCCTGAATAACTTTT2160.10137370760255876No Hit
CTGAGTGGGGGGGGACAAAGAGTTTTTATACACTTCTAAATTGCTTGCATTT2160.10137370760255876No Hit
TGTTAGGGACCATTGTATGATACCCACTGAAAGCAACAAAAACTAAGACTGA2150.10090438488217653No Hit
CACTTAGGGCAATCTAAGCTTATTTCTTCCTGTATCTCCTTTTGCAATAGAT2150.10090438488217653No Hit
TGACACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2150.10090438488217653No Hit
CACTCTGGGCGATCTTTGTGAGGGAATGATCATAAGTTGGACAATGATTACT2150.10090438488217653No Hit
GCAAAAGGGTATTTCCTCTCATATATGCTTAGGTTTAAGTCAAATAGCCTGA2150.10090438488217653No Hit
TTTTCCGGGGACACCCCAGCGGTGATGAGCACACCTCACACCCAGATCTTGG2150.10090438488217653No Hit
ACTTACGGGATCCCTGCATAAAATAGGAACTCTCAGAGGACTGTATTCTATG2140.1004350621617943No Hit
CAAGAAGGGAAGTATCTCAGAGCTCATAGAAGTTGACAGTTGATTAAAGTAC2140.1004350621617943No Hit
TGCTAAGGGCACATTCATAGTATTTTGGTAACTAAGGTATCCATAGGCTCAA2140.1004350621617943No Hit
CCAACTGGGGTAAATGAAAAATCCCTAATTCAGTGAATTCACCTAGTCACTG2140.1004350621617943No Hit
TGTTTAGGGGTCCCTGAAAACCACCAGTAATTTGAATTTGACTACTCTGGGT2140.1004350621617943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)