FastQCFastQC Report
Sat 31 Dec 2022
SRR5845025.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845025.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211419
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT8230.38927437931311754No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC7460.352853811625256No Hit
TGAAAGGGAATATTCAGATGCAACAAAATTACAGATTGTTAAATTTCACTGA3210.15183119776368256No Hit
TTTTATGGGGAGCACAAAATAAATGGTGTTTTGGGTGAATATATAGTTTTGA3030.14331729882366295No Hit
TCAATTGGGACGGGGGGTCAGGCCTGTTGCATTTTACATTGCCAGACTTTTT2990.1414253212814364No Hit
GATTACGGGGAGTGATTGGGAGAGAATAGGAAGAAACAACAAATGCAAATAT2980.14095232689587975No Hit
ACATGCGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGCG2970.1404793325103231No Hit
AAATTAGGGCAGTGGAAGCTTTGCAACAGTTGAATGATTTAAGATTATTATG2960.14000633812476646No Hit
TCAGCTGGGTCAGTAAACTATGGTACATTCCCACAATAAAATATAATGCAGT2900.1371683718114266No Hit
TTGCCTGGGACAGTTTTGTGGTAACCGAATAGTTAGTGGTTCTGATGACAAC2900.1371683718114266No Hit
CACGCCGGGGCAGAGCTACATGGCTCCTTCATGTTACAAACTTCTAAAGTAA2860.13527639426920002No Hit
GCTACGGGGATGATTATTTGAAATACACTTGTGTTAGTAACTGCTTCTATAA2850.1348033998836434No Hit
AGTCAAGGGATTTTCAGATATGATTGTTTATGTAAACTAAAAAATTACTGCA2820.13338441672697346No Hit
GTAAATGGGGCAAGTTGTCATTCATTGAAAATGATTCTTACCTTTAGCTGAT2740.12960046164252031No Hit
GTAGTTGGGCCTAAATAAATGAGCTGTTGGAAGAAAAGAATCACAGTACTTT2730.12912746725696364No Hit
AATACAGGGGAATACCATGATCGATGATGTCCCATGCTTCGAGGAAAACCAA2720.12865447287140702No Hit
ACGGCTGGGTCCTTCATTTCTGTCATTCAAGCTCAACGGCTATTGTGGTGAC2710.12818147848585038No Hit
TGTAACGGGATTCCTAAGGCAAATAAATGTACAAAAATAACTTACATTCACC2710.12818147848585038No Hit
GACGTTGGGTTTGAAAATAAATTACAGATATTAATATACTTCTCCCTGAGTA2700.12770848410029373No Hit
AATTGTGGGATGGTTTATTTAGAATTTAAAATACCAGTAAACTAGATCTGAT2700.12770848410029373No Hit
AGTCCTGGGCCTCCGGTCCCGTAGTCCAAATCAAGTTATTAAATTTAAACCT2690.12723548971473708No Hit
CTCGTTGGGGTATATAATAACTATGATCAAAAAGCTATTTGGAATAGTTTGA2680.12676249532918044No Hit
ACATCAGGGTCCTTGCATTTAAAAGTTATTGATACTTACTGTCTTTTTAGAG2680.12676249532918044No Hit
TATGCTGGGGACCTTTTCTTCAGGATTACAGGTACACTGGATGCAGCCATGC2680.12676249532918044No Hit
AGTCATGGGCCATAAAACTGTCTGTATAACTCCCTGGGTTTACAAGCTCCTG2670.1262895009436238No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC2650.1253435121725105No Hit
GGCCCAAAAAGTTATTAGACCCAATAAGATAATTCAGGAAGTCCAAGAGGTC2650.1253435121725105No Hit
TGTTAGGGGGTCGCTATTAGTTGGGAGTTGCAATACATTGTTGGCCATAAAC2630.12439752340139723No Hit
ACGCAAGGGAGCCCGAGTAGGGGCTGGTGACAGTCAATTGAAAGGAGTCTCC2630.12439752340139723No Hit
TAACATGGGTGTGCTAAGATGAATAGAACACAAGGAAATAAAAATCTCATAG2600.12297854024472729No Hit
ACTACAGGCAAGATCTTCATAATAATGCAGTGTGTTCTACTGATTGCATTGT2600.12297854024472729No Hit
CAGCCTGGGGGATCTATAACGGGGATGGCCCACCATAATTACCCCCATACTC2590.12250554585917066No Hit
CTACTGACGGGATTCTTCAGATAAATAGTGGTGAAACTAGACTGAGTGATTT2570.12155955708805735No Hit
GCGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2570.12155955708805735No Hit
TCCAATGGGCACCTCAATGAATTTTCACAAATCAAGCTCATCCATGTAACTA2570.12155955708805735No Hit
CGTTAAGGGCAGAGGAATAATTCACAGTGGAGATGGGGTAGATAGTTTTGTA2560.12108656270250072No Hit
CAGCCCGGGAGATAAGATGAATGGCTTTCTAGAAGGTGTTGCTTTTTGTTTT2560.12108656270250072No Hit
ACAAAAGGGTCTCTATGGATTACCCAGACAAGACACTACATCCATCCAACAA2560.12108656270250072No Hit
TTAATGGGTGATTTGCAAAATCACAAAACTAAGTTGTCAGTTATCACTGTAG2560.12108656270250072No Hit
TCAGTAGGGTCTAGTGTTTTGCTTTCTTTTCCTTATAATAAATGACCCTTTT2550.12061356831694409No Hit
TCTCCGGGGGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAAC2540.12014057393138744No Hit
TTCCCTGGGCCTACAGTTGGTATTACCTCTGTGCTCAGTGCCAGGCAAAACA2530.11966757954583078No Hit
AACTAAGGGGCTTTCCAGGGCAACCGTACTTTCCTAAACTTCATTTGTCCCA2500.11824859638916085No Hit
GGTTCAGGGTAGTCTCAAATAGGTGCTTCCCCTGAGGTGCAGGGGAAGACCA2500.11824859638916085No Hit
TCGTCAGGGATGTTGGAAGATTTGGGAATGGATGATGAAGGAGATGATGACC2500.11824859638916085No Hit
GGTATTGGGATGGAGTGTCCGAAATATGCAGATTCATGAACAGAAAGTAGAT2490.11777560200360421No Hit
GTGGCGGGGATACAAAGCTATGTAATGTTACCTCTTGACTCGCTTTTGAAAG2480.11730260761804757No Hit
GGAACGGGAGTGAGTACAGTGTTGAAAATGAGAACTGGACTAAAGGGTCCTT2470.11682961323249094No Hit
GCCACAGGGTTCTATGAAAAATGGCATTGATATTTTGATAGAAATAGCATTG2470.11682961323249094No Hit
AGAACAGGGAACTGGTAAAGAATTGGTTCTCTGTAAATACCTGCTGCTTGAA2450.11588362446137764No Hit
CTAGTAGCGGGTATCTACAAATGCAAGTAAAATTTCCTGTAACAGCATTTCT2450.11588362446137764No Hit
CGTTCAGGGGAGTTGGGAAAACGGATCCTGTCACAAGAGGCATTGAGATCAC2450.11588362446137764No Hit
AGTTCAGGGATTAAATGGAATGGAATAGAGATTTAATAGAAGTGTGGTTCGT2430.11493763569026437No Hit
ATTATGGGGATCCCTTATGTGAAATTTTGTTTTTCCTAAGTTGAAAAATGAT2430.11493763569026437No Hit
TTGAACGGGATCCTTGAAAGACTTAATAGAGATACATGAGACTAAGTTAATC2420.11446464130470771No Hit
ATTTAAGGGTTTCCACTCTTGAAATTTACCTGTTCTATGTGTTTTATCTTCT2420.11446464130470771No Hit
ATTGATGGGAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCA2410.11399164691915106No Hit
AGCCAAGGGAGAGGGGAGGCGCAGCCGGGCGGAGGGCCCACGAGGGCTCAGC2410.11399164691915106No Hit
TCAAGTGGGATTTTGGTTTGTGGAGGAACTCAAGTCATTAATGAGGTGTCAG2400.11351865253359443No Hit
TGTTAGGGACCATTGTATGATACCCACTGAAAGCAACAAAAACTAAGACTGA2390.1130456581480378No Hit
GCCAGAGGGATATCTGTGGACTGAATGCAATCTACGGGCCATCAATTCGTGG2380.11257266376248114No Hit
TACATAGGGGATGATCTCAAATGCTCCTCTCAGTGCTAAGATGTTTGATGTT2360.11162667499136786No Hit
TCGCTTGGGAACCCATGTGATAGCAGCCTCGAAAGAAGCATTTTATACCTGG2340.11068068622025456No Hit
CGGTGCGGGCATGAATTATTGAAAAATAGTCTATGTTCCAAGTCCATGATAT2340.11068068622025456No Hit
TATAATGGGGATTATTAAAAAGTCAAGAAACAATAGATACTGGGAAGCTTGG2330.11020769183469792No Hit
TCACCACGGGGATAATCATAAGGTGTTTGTTAGGAGCGCTAGACCACCTGGA2330.11020769183469792No Hit
TGCAAAGGGAAGTTTGGATCTTTTAAAATCAAATACACATATAAAAGCAATT2300.10878870867802798No Hit
AGTTATGGGAGGGAGGAAATTTTGGAGGCAGAAGCTCTGGCCCCTATGGCGG2300.10878870867802798No Hit
CATATACGGGAGTTTATGATGCAAATCTGCCAAGAGAGATGTATGTGTCACT2290.10831571429247135No Hit
CTTTGACAGGGAGTCCACTTGGCTGTACATAATAATGATCCATCCTGCTTTG2290.10831571429247135No Hit
TAACGCGGGGGACCTTTCCGAGTTGGCTGCAGATTTGTGGTGCGTTCTGAGC2290.10831571429247135No Hit
GGAAATGGGCATTTTGGTTTTGTAAAAGCTGTTACTGAACTCAAGAAAATAT2280.10784271990691471No Hit
AATTCAGGGGCAAGAGCCCAGGAATATGCGGAACTAGGTCCACAGGAGAATG2270.10736972552135807No Hit
TGGTTAGGGAAGGAGAAGATACCCTTTCTCTCATAAAAACACTTAGAGCAAA2260.1068967311358014No Hit
TTATCTGGGAGTATGTATTAAGTCAAATTTTATAAAAAAGAAAGTTGGTCCT2250.10642373675024477No Hit
GGCGTGGGCCTACACACAAGCGTTAAACGGTTTCTCTGGCATGCTCATTTTC2250.10642373675024477No Hit
AGATATGGGATAGCGATGAATCACCTGAAGCTGGTAAAATGCTTGAAGATGA2250.10642373675024477No Hit
CCACCCGGGAGGTGTGTTATAGTTTTCTTCTGAAAGATAGTTTTTCTGTCTC2250.10642373675024477No Hit
AAAATCGGGAGTGAAACATTTTTTAAAAGGAAAAATAATAGCCCTTCAAGGT2240.10595074236468814No Hit
GCATAAGGGCAATAGAACAGTGGCTATGGTCAATATTCCTAGGAATGGGAAC2240.10595074236468814No Hit
GATGTCGGGAACAAAATAGAGTTTTAATTCCTGATTAGAGAGCCATAACTGT2240.10595074236468814No Hit
GCCAATGGGAGTCTAAAAAACCAGATGAAATTGAAGGTTGGGAGCCTCCAAA2240.10595074236468814No Hit
TTGGATGGGGGTTCATATAAATGTACAAATAGCAGTTAGTATCAGTATAGGT2230.1054777479791315No Hit
GCACCTGGGACTACAATTTGAAGTCAGAAATGCCACTTGGGTAGCTAATGAA2230.1054777479791315No Hit
CATGCTGGGATATGTTCTGTCATAGAAATTGTTTTCAAGCCAGTTTCCTTTT2230.1054777479791315No Hit
TGTAGCGGGATGTTTCAAAGGGAAAAATGTAGTTGTCTTCTGTACCTGTCAC2230.1054777479791315No Hit
AGTACTGGGGTGGGCAGTTTCAGAAAAGAAAACAAACAAACAAAAATCGACC2230.1054777479791315No Hit
TTAATGGGGAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGTAAAG2230.1054777479791315No Hit
TGCCATAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG2230.1054777479791315No Hit
CAAGAAGGGCAACAGAAATAACAAAGTAGCACAGACTGTGGCTTAAACAACA2220.10500475359357483No Hit
ACCATAGGGTGGAGAAGTCAATGGTACCTACTATCAATTTGGCAAACATTAT2210.1045317592080182No Hit
ATTCTAGGGGACCGGGAGTGGGCGGGCCATTTCTTGTTCTCTCTCCCGCTCT2210.1045317592080182No Hit
AATAATGGGTAAGCCTTTTCGATGTGCTGCTGGATTTGGTTTGGCAGTATTT2200.10405876482246157No Hit
TGCTATGGGGTTGGTAGGGTGATACTTTATAGAAATAAAGTAAATTGTGTAA2200.10405876482246157No Hit
GCATGTGGGCTGGACAGAAGGAATGTGTCAAACCGTGGTCTGCCAAGAAATA2200.10405876482246157No Hit
TGCTAAGGGCTATATGGATAGATAGTGTAACATTAGTCTCCTGACACAACAT2200.10405876482246157No Hit
CAGATAGGGAGTTGCTTTAGGGTCCATTTTTTTATGTAATCACTTACTCAGT2200.10405876482246157No Hit
GCCTTGGGGCATCCTAACGTGTTGGATTTTTTGTTTGTTTCACATTTCCTGA2200.10405876482246157No Hit
TTTAGCGGGATTGCAACACTATCTTGTCCTCCTGATGTCAACTGACAAAGGA2200.10405876482246157No Hit
ATCTACGGGCATGGTGAATTAACTTTCTGATTTGCTGCTGGATTCAATGTGT2190.10358577043690491No Hit
TGGCTGGGGGATCTACGATTGGGGAGAGTGCATTCCCCACCCCCATGTTACT2190.10358577043690491No Hit
AAATATGGGATTCTATAATTGGTAGCATATCATAATTTAATTGGCAACCAGT2190.10358577043690491No Hit
CTCCCAGGGCAGAAGCCTTCCATCTGGAGGATTAGGTGACCTTAAGGTCCCT2180.10311277605134826No Hit
AATTACGGGGATCTTACAAAGGGCACATATAATCAGGGAGACAGGATAGACA2180.10311277605134826No Hit
GCAAAAGGGTATTTCCTCTCATATATGCTTAGGTTTAAGTCAAATAGCCTGA2180.10311277605134826No Hit
AAACCAGGGCAGGACACGGTGGGTGTCAGGCCTGGTCAGGCAAGGGGGCTTT2170.10263978166579163No Hit
TGCAAGGGAGCTGAGACCTGAAGAATAAGGGACAATTTTACCCTGACTGCTG2170.10263978166579163No Hit
TCATGCGGGTGCGTTGGGGTCCCAAATCCTTTGAATACTGTGCCAGGATTTT2170.10263978166579163No Hit
CTGAAAAGGGGAATCTAGTCCATGAAATATTTCTTAAACAATTCAAGGATCA2170.10263978166579163No Hit
ATTGATGGGGGCTTTTCATAATTGTCAAGTTCATTTAGTTCAGACGGATTCC2170.10263978166579163No Hit
AAACTTGGGAGAGACCCTGCCGCACCACAGTGCCAATGCTTGTCAGACACAT2170.10263978166579163No Hit
ATACTCGGGTAATTACATATTTATTTACCTGATAAATATGTATCTAGTTCTA2160.10216678728023498No Hit
CGTGCTGGGTTCCTGAGTTACTTCATATAGAATAATGGCCTCCAGCTCCATC2150.10169379289467834No Hit
TTTCGCGGGTCTTTCTTCCTGGAGAAGAGGAGATTTTCTCTTTTGAGAGTGG2150.10169379289467834No Hit
CAGGTTGGGATATTTGACTTATTTTCATATTTATTGTCTACTTCCTTATGAA2150.10169379289467834No Hit
GCATGCCGGGGAGGTCAAACTGGCATTTTTCCAGCCAACTACGTAACCATGA2140.10122079850912169No Hit
TACACCGGGACAGAGCAAACTCTGCATGAACAAGGAAGGAAGGAATACATTC2140.10122079850912169No Hit
GATTAGCGGGCTGACTCATTTCCTGTACTTTCTCTTTCTGTCCCTTCATAGC2140.10122079850912169No Hit
GTCTAAGGGATAACAAAAAATAGAAAATGTTGATAGTATGATAGATAGTGAA2140.10122079850912169No Hit
AGACGGGTAGATATAGAATAATGTCTTCTGCAAACAGGGATAGTTTGACTTT2130.10074780412356506No Hit
TGCAAGGGGATCATTCATTCATTCTTTTAGCAAACCAAAGACAGTGTATATA2130.10074780412356506No Hit
CAGCGAGGGGAAATTGTAAAAAAGAATATGAATATTTTACCTTTATAACTAC2130.10074780412356506No Hit
TAGCTTGGGATTTCGGCCAAGTCATTCTTTATAAGCAGAGGCAAATATCATG2130.10074780412356506No Hit
CTGAGTGGGGGGGGACAAAGAGTTTTTATACACTTCTAAATTGCTTGCATTT2120.10027480973800841No Hit
AGCTTAGGGATGGAACTGATGGAAAAGAAACTTATGGATTACATTGATCAGC2120.10027480973800841No Hit
TGACACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2120.10027480973800841No Hit
TACGTCGGGATGGCTGAGGCCCACAAAGAGGCCATGAGCCGCAAACTGGGTC2120.10027480973800841No Hit
ATTGGCGGGAGAGGCGGACAAGATGGCGGCGGCAGCTGTACAGGGCGGGAGA2120.10027480973800841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)