FastQCFastQC Report
Sat 31 Dec 2022
SRR5845024.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845024.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181342
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT5120.2823394470117237No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC5100.28123655854683416No Hit
GGCATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG2210.12186917537029479No Hit
CGGGATGGGAATGAAGCAGCTAGTAGACAACTGTCTCTGAGACAGCAAAAAT2100.1158032888134023No Hit
GGCCTAGGGGGCAGTTGCCCCCCGCGCTGTGCGCCCGCCTCGCTGCGGGGCC2070.11414895611606798No Hit
AAATGAGGGAGATGTGGCCTTGAAAACACATCATTGTAAAGGAGTTTAGATT1870.10312007146717253No Hit
CGACGCGGGTGTCCTACATGAGGCGGCAGGCAGCCCTGGGGGTCAAGAGGAA1870.10312007146717253No Hit
AGTGCTGGGAATATTTTTCCTGTTCCAAATGTTCTTTTTATGTTGATTTTTG1820.10036285030494865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)