FastQCFastQC Report
Sat 31 Dec 2022
SRR5845023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181019
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC4830.266822819704009No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT4700.25964125312812464No Hit
GGCCTAGGGGGCAGTTGCCCCCCGCGCTGTGCGCCCGCCTCGCTGCGGGGCC2050.11324778061971394No Hit
ACTACTGGGAAAAGGCAGCCTGTTTTTTCTGCTTTTATTGTATTAACAGCTG2030.11214292422342406No Hit
CGGGATGGGAATGAAGCAGCTAGTAGACAACTGTCTCTGAGACAGCAAAAAT1990.10993321143084427No Hit
AAATGAGGGAGATGTGGCCTTGAAAACACATCATTGTAAAGGAGTTTAGATT1960.10827592683640944No Hit
AGCGATGGGATTGCTGCATTTATATAGTGACAGCTGCACTGTACCATAACTC1870.10330407305310493No Hit
TGTCAGGGGGAATTTCAGAACTCAAAGACAAGACTTTTGAATCAGCCCAGTC1860.10275164485495998No Hit
CGACGCGGGTGTCCTACATGAGGCGGCAGGCAGCCCTGGGGGTCAAGAGGAA1820.10054193206238018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)