FastQCFastQC Report
Sat 31 Dec 2022
SRR5845022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180671
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT5120.2833880368183051No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC4620.2557134238477675No Hit
GGCATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG2070.11457289769802569No Hit
CGGGATGGGAATGAAGCAGCTAGTAGACAACTGTCTCTGAGACAGCAAAAAT2010.11125194414156117No Hit
TTTAATGGGTTCAGAAGTTAATTAAGTCAGAGAATTATCTATGAATTGGTTT1920.10627051380686442No Hit
AAATGAGGGAGATGTGGCCTTGAAAACACATCATTGTAAAGGAGTTTAGATT1880.10405654476922141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)