FastQCFastQC Report
Sat 31 Dec 2022
SRR5845015.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845015.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188019
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC5380.2861412942309022No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT4550.2419968194703727No Hit
TAAGCAGGGAGAAAAGACTCTTGCAATTTGTAACAATAAATATCATGTTAGA2370.12605109058127104No Hit
CACACTTCGGGTGGTTGAAATATATTTGACATACTGCATTAAATGGAATATA2260.12020061802264664No Hit
GAACTAGGGGATTTTGCAGAGTGAGACAAAGCGGAGAACGCTGGTGGGCCTG2240.1191368957392604No Hit
TGCTAAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2220.11807317345587415No Hit
AACACGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2150.11435014546402225No Hit
TTAGGTGGGACAAGATAGCCTGTACAATGATGTCAATTGCACCTTTGCTTGT2150.11435014546402225No Hit
TATGAGGGGATTCTCCAAGAGGAAATGGGATGTGAATGAACATGCTTTTTTT2070.11009525633047725No Hit
GGCTACGGGATTCCAACTGGCCCACCATCCCGCAAGTGTACCTCAATGGCGA2020.1074359506220116No Hit
CGTCATGGGGAGTATAACTTTTTGTTTTCTTCAACAGTGAAAAACCCAGATT2010.10690408948031847No Hit
CGCGAGGGGCAGGCATCTGCCACCATGCCCGGCAAATTTTTGTGTTTTTAGT2010.10690408948031847No Hit
GTCAGAGGGCTGGCCAGGCTGATGGAGCAGTGTGCCTCCCTGGAGGAGCTGA1970.10477664491354596No Hit
CCAATGGGGATGGAAAATGCAGTGGACACCAAACCAGACATGGACAGTACAA1910.1015854780633872No Hit
GCAAACGGGATGACATGATTGTATATCTAGAAAACCCCATTGTCTCAGCCCA1900.10105361692169408No Hit
AATAAGGGAAGGGTTAAGGAATGGTTTGGCCATTCTGACTTGTGCCATCTCT1900.10105361692169408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)