FastQCFastQC Report
Sat 31 Dec 2022
SRR5845014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188574
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC5300.2810567734682406No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT4530.2402239969454962No Hit
GAACTAGGGGATTTTGCAGAGTGAGACAAAGCGGAGAACGCTGGTGGGCCTG2260.11984685057324976No Hit
GTATCAGGGGGAGATAAAACATTTCAGATAGTGGGGTCCAATATTTGAAAAG2240.11878625897525638No Hit
TAACAAGGGCAAGGAGGTGGCTGAAAGTGGTGTCCAGATGTCCTTCCGAGGA2230.11825596317625972No Hit
CAAGCAGGGAAACATATATGTGCCTAACACTGGAGCTCCCAAATTTACAAAA2210.11719537157826634No Hit
CACACTTCGGGTGGTTGAAATATATTTGACATACTGCATTAAATGGAATATA2160.11454389258328296No Hit
GCGCTTGGGGGCAAGATGGTCAGGTCCATCTCTATTAAAGAAATAAATAAAT2150.11401359678428627No Hit
TCACGCGCGGGACGTCTGAATTTTTTGTAGTTACATCCTGTATTCCATGTCC2140.11348330098528958No Hit
ACGCATGGGTACCTTCCCAGGCTCCAGTCCCTGTTCCTCAGCATCCCCCACC2140.11348330098528958No Hit
TGCTAAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2100.11136211778930288No Hit
CGTCATGGGGAGTATAACTTTTTGTTTTCTTCAACAGTGAAAAACCCAGATT2100.11136211778930288No Hit
CGCGAGGGGCAGGCATCTGCCACCATGCCCGGCAAATTTTTGTGTTTTTAGT2100.11136211778930288No Hit
TTAACAGGGTGGGGAAAAGATCTACAACAAGAAAGGATACCCACTTTCACAA2000.10605915979933606No Hit
ATACTAGGGCACGACGCTCTTCCGTTCTGCTAACGGGATTCTTTCTCAGTTT1990.1055288640003394No Hit
TTAGGTGGGACAAGATAGCCTGTACAATGATGTCAATTGCACCTTTGCTTGT1960.10393797660334936No Hit
AACACGGGGCTACCTTTTCTGCTAAAAATTTTAGTCACATTGACTGGAGTAC1950.10340768080435267No Hit
TATGAGGGGATTCTCCAAGAGGAAATGGGATGTGAATGAACATGCTTTTTTT1930.10234708920635929No Hit
ATGCCTGGGGACAACTTGCCATTATCTGATGTTACACAATTCAATAGCAAGC1930.10234708920635929No Hit
TAAGCAGGGAGAAAAGACTCTTGCAATTTGTAACAATAAATATCATGTTAGA1920.10181679340736263No Hit
TTGATTGGGGGGAGTGGTGTATTGAGAACGATTCTGAAGAATTCTGGAGAAA1910.10128649760836594No Hit
CGGGCTGGGATAAAACCCCAGTGAAGTTTATTGAAGAAGGTAGTGTTGGCTT1900.10075620180936926No Hit
ACGCACGGGAGGAGATTGATGGCATTGAGGAAGATTGCAATGGAGATCATGA1900.10075620180936926No Hit
CAGTTGGGGGAATCAATGGCCCTGTTGCCAAGTAAAACCTCCATTGCATTTA1890.1002259060103726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)