FastQCFastQC Report
Sat 31 Dec 2022
SRR5845013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188320
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC4760.2527612574341546No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT4280.22727272727272727No Hit
CACACTTCGGGTGGTTGAAATATATTTGACATACTGCATTAAATGGAATATA2290.12160152931180969No Hit
AACACGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2200.11682242990654204No Hit
CGTCATGGGGAGTATAACTTTTTGTTTTCTTCAACAGTGAAAAACCCAGATT2180.11576040781648257No Hit
GGCTACGGGATTCCAACTGGCCCACCATCCCGCAAGTGTACCTCAATGGCGA2140.11363636363636363No Hit
CTTCATTATTGCAGCCCTAGCAGCACTCCACCTCCTATTCTTGCACGAAACG2110.11204333050127444No Hit
ACGCATGGGTACCTTCCCAGGCTCCAGTCCCTGTTCCTCAGCATCCCCCACC2110.11204333050127444No Hit
CGATTGGGGGTAGTCAATCACTAACCTGACATACTTTGCCTACTCATTGATG2070.1099192863211555No Hit
TTAGGTGGGACAAGATAGCCTGTACAATGATGTCAATTGCACCTTTGCTTGT2060.10938827527612574No Hit
TTGGTCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2020.1072642310960068No Hit
ACACAAGGCCTTTTGAAAGATCCCAAACTACTTTCCAAGCCTTTATTTTGCT2020.1072642310960068No Hit
ACGGTTGGGAGACAATCACAGATGAAAGAAAACGAAAGTAATTTGTTACCAG2010.10673322005097705No Hit
GTCAGAGGGCTGGCCAGGCTGATGGAGCAGTGTGCCTCCCTGGAGGAGCTGA2010.10673322005097705No Hit
AATACGGGGACCGTCAGAGTGATTGATGTGTTTGCTATGCCACAGTCAGGAA1970.1046091758708581No Hit
TAAGCAGGGAGAAAAGACTCTTGCAATTTGTAACAATAAATATCATGTTAGA1930.10248513169073917No Hit
CAAGCAGGGAAACATATATGTGCCTAACACTGGAGCTCCCAAATTTACAAAA1920.10195412064570944No Hit
TGTCAGGGGCAGCAGAGTTGGGGCTGCCACAACAGACTGGTCAGCCTTCTCA1900.10089209855564997No Hit
ACTTTCGGGCATTTTCTAGATCCACACCTGGATACTGCCCATGTTGACGAGA1890.10036108751062021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)