FastQCFastQC Report
Sat 31 Dec 2022
SRR5845009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167561
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC4950.29541480416087273No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT3900.2327510578237179No Hit
CCAACTACTAGGTCTTGATTGTTCCAATTAATGATATATTTGATCTTTTGGA2790.16650652598158283No Hit
ATCAGCGGGGGGAAGAAGAGATTGAAGCTCAAGAAAAAGCCAAACGGGAAAG2740.16352253806076592No Hit
GTAGACGGGGATTTGAAGCTGGAGGTACAAAGTAAAAGTCCGCCAAACCCAT2680.15994175255578563No Hit
GTAAACGGGTAGCTTAAAAATGAGGGCATTTGCATGATTGAGGGATTGTGTA2420.1444250153675378No Hit
GTTGCAGGGGTCCACGTCCATTGCTCAGCTCAAAGTGGTACAGACCAAGTAT2400.14323142019921103No Hit
GCAACAGGGATTTGCAAGTTCAATTTGAATCTTTTTTTAGTCTGCTTGTACT2370.1414410274467209No Hit
TTTACCGGGATGTTCACGATTCTTTGAGCACTTTACTACTTTGTGATGCGGG2330.13905383711006739No Hit
ATACCAGGGTTTGGGAAGAAAAGTAATAACACAGCTACAAGTTAAATTGAAA2290.13666664677341386No Hit
ATATGCGGGAACGTCACAAAATTGGGTAAGGAATTAAAGGAAGATTGCCTGG2270.1354730516050871No Hit
CTTATCGGGGTTTTGATCCTCTTCAAAAGGTGGTTTATAATCAGCTGTAGAA2200.13129546851594345No Hit
AGCTGGGGGGGCGCCGCCATGGCCGACGTGGAAGACGGAGAGGAAACCTGCG2180.1301018733476167No Hit
TGCTCTGGGCTGACTTCTCCTCTCTAGCTGTGGAAGTCCTAGATGGCATATT2170.1295050757634533No Hit
CTGAGGGAGAACTGAATTCCTCTGGAATTGAGTGATGGACTTCACAACAACT2170.1295050757634533No Hit
TTAACTGGGGGCTGTCTTGAATGCTCAAATGCTGATTTTGTTTTCCTTCACA2160.12890827817928993No Hit
AAGTTAGGGTACATTTTGGTTCAGTTTATTTCCATGTTGAATTAGAGTGGAT2150.12831148059512654No Hit
AAACTCGGGGTTGTTGAATTAATTTATCTTTTAGGATTCTGTTCGGTAAGAC2130.1271178854267998No Hit
CTATCAGGGCGTCTTGAAAATAAATGTGTTTAGAATTTTCTAAGAGAGAATG2130.1271178854267998No Hit
GCGTAAGGGAGAAAACGAACATGATGAACACGATGAGAATAATGCTGAAGCT2120.1265210878426364No Hit
GCCGACGGGATGCCAGTTAAATGAATGTATTTTCAGTTTACTTTCCATTGAT2110.12592429025847304No Hit
TACAATGGGGTGGTTTCAATCTAATTTTTTCCCAGACTACTATTTTCTTTTT2100.12532749267430965No Hit
CATTCTGGGGGCGTGTGATGTGTGATTAGGCTTCTTGCTCTTAAATCCAGGT2100.12532749267430965No Hit
TTTGCAGGGTTCACATGTGCCAGTATGTCTAGCACATGTGAACCTTGCAAAA2100.12532749267430965No Hit
GACAATGGGGAAATCTTTTAGAAGTTTGTAGAATGGTAATATCAGAGTGCTG2090.12473069509014627No Hit
TATTGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2090.12473069509014627No Hit
TGTTTTGGGGGGAAAACGGGCAGGGGAAAGTCAGGCCCCACACTGTCTCCAG2090.12473069509014627No Hit
TGCTGTGGGGGTTCTGTAACTGGTATGTTACAACTGAAAAACTTTTAAAATG2080.12413389750598289No Hit
GCTCAGGGAAATGGTACATGTGTTGCTGTCTTGTCTAAAACTTTTAAAGGCA2070.12353709992181951No Hit
GTAGACGGGCAGCCGTGGCCCCGACTCTGGCCTGGTGCCCCTGGCCTACGTG2070.12353709992181951No Hit
GCAACTGGGGTTGGGGTGAAGCACACTTATTATCTTCAGTTGCAGTGATTTC2070.12353709992181951No Hit
TTGCAACCAAGCAAACAAACAAAAACCAAAAAACAAATAACAAACCAAGAAA2070.12353709992181951No Hit
CTGGCAGGGCATAGTGATATTTGCAAATTTGGACAACTTAAAAAGACTTGCA2060.12294030233765613No Hit
GTTGCTGGGATACAAACCAAAATTTTAATCCATACAGGAACCAGAGTCATTA2060.12294030233765613No Hit
AGCTAAGGGGCAGCAGCCCTAGGGGGTTGTCAGAATCACATTCTGGTTAAGT2050.12234350475349276No Hit
GCAATGGAAATTTGTTTTGTCATATTTGCTTTCACTGTCTATTATCTGTTTA2050.12234350475349276No Hit
GCTGTGGGGGCTACATTGTTTGGATTTCTATAGCTTTGCAAAATAGTTTAAA2040.12174670716932938No Hit
ATTTTTGGGCAATGTATTGTCTTGAAAATACAAAACTATTGTAGTATGTATG2040.12174670716932938No Hit
TTTCCCTGCAGTAGAAGATACTTTTGAATGTGAAATCAGTATTGAGTATTTC2010.11995631441683924No Hit
AGCTTTGGGGCCTCCTGGTGTCGCACATCCATGGGAATAATGAATTTATTTG2010.11995631441683924No Hit
TAGACTGGGACTATGAAAAAAGCTGCAGACTGTGTAGACATATGTTTTCATT2010.11995631441683924No Hit
GTAAGCGGATGAACAAGAGATGTCTTTCTGTTTATTTGGATCTTTTTTCATT2000.11935951683267586No Hit
TATCTGGGGGGCATCTTCCCGGAATCAGATTCAAAGGATCCTGGAGGTGATG2000.11935951683267586No Hit
TTGATGGGGATAGCAATCTTCTCAACAGAAGTAGGCATGGAAAAGACTTCTT1980.1181659216643491No Hit
AATATAGGGAGCTCACTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCT1980.1181659216643491No Hit
TACTGCGGGGAGCAGACTGCTGCGGTTCCTGGGTGGCGGCGGCATGGAGGCG1980.1181659216643491No Hit
TTGCACGGGACCCTTTGTTTCACCCTGTCAGTCTCCAACCTGACGAGCAGAG1980.1181659216643491No Hit
CACCCTGGATGAAAGGATGTTAAATATTTATTTTAGAAATGAGGAACTTTGA1960.11697232649602234No Hit
GTCGCGGGGGTGTGCAATTTTCTGCAACATTCCAGGTTAGAAAACTGCAATC1960.11697232649602234No Hit
TTAAAGGGGTTATAATCAAGCTACAAATAGGTTTTCTTAAACCAGAGATGCA1960.11697232649602234No Hit
GCACTTGGGAGTGTATTTGTACACTGATAAACTGATCTTCCGAAGAGACTGT1960.11697232649602234No Hit
TTAGCAGGGTGGATTAGAGCTGAGAAAGCAGGGAGGATAGGATTTGAGTAGT1960.11697232649602234No Hit
TAGCTTAGGGGGTCCTTCATTTGAGCAGCTTCCTTTATGACTCATCTTTCTA1960.11697232649602234No Hit
GTGCATAAGGGGGGCTGATTTGGGTCTATTTTGCACTTGTGTGGTTCAGGGG1950.11637552891185896No Hit
CATTGCGGGCCGTGTTGGCCAGGCTGGTCTCGAACTCCTGACGTCAGGTGAT1950.11637552891185896No Hit
GTCGTTGGGATGATTAATCAAAAATTGTAGAATATAACAAGTTCGCGTAAAT1940.11577873132769557No Hit
TCAGATGGGTCCATGGCACTGATTTGGGCCTCTGCTTGCTTCTCTCTGCAGA1930.1151819337435322No Hit
TTGGTGGGGCTTATTTAACTCTAGGCATGTTGACTTTTCAAAGCGGTTTCCT1930.1151819337435322No Hit
ATTGCAGGGATAGTTTTAGTTGTCACACTCATCAGAGATTGCACACAACTAT1930.1151819337435322No Hit
TTGCTTGGGACATCTATCTTTACTAGAGGGCAGGATTTGCTATCTCTCTGGA1920.11458513615936881No Hit
TGTTGTGGGATTAATTGTGTTAAGTATCACTCTTTAACAAAGACCAGACCTA1910.11398833857520543No Hit
TACTTCGGGCAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAG1910.11398833857520543No Hit
GTAATGGGGTGCTGCTTGTTGCAGTGACTAACCACCAATGTTAACATCTTCA1900.11339154099104205No Hit
TTTCCAGGGGGAGGGTGAAATTTAGCCATCGGTGTGTGGCAGGGTCATGAAA1900.11339154099104205No Hit
GCCTCAGGGAAATACAAATCAAAACCACAGTGAGATACCATCTCACAACAGT1890.11279474340687867No Hit
CTGATAGGGATCACATGGCAGTTCTCTTTTTAGTATTTTGAGGAACTTGTAT1890.11279474340687867No Hit
GCTCTTGGGATCCTATTATATAATGGAGATGTAGACATGGCCTGCAATTTCA1890.11279474340687867No Hit
GCTACGGGGGAGAACTTTTTTTTAAGAACATGGGAAACTGAAAGATCATTTT1890.11279474340687867No Hit
GTTCTTGGGGCCTCGGAGCTGAAGCCCGTACTCAAGATGGCGGCTCCGGGCG1890.11279474340687867No Hit
GTCGATGGGCTGTGCGATTCTGTATCTTGGGAAAAGAGTCCTTTTATGACAC1890.11279474340687867No Hit
TGCAATGGGGGAACTAGGTAATTCACTCTTATGATAAAGAGCTATTAAAATG1880.1121979458227153No Hit
TGTCAAGGGGTAAAATAAGTAAGTTGTGGTAAATTCATTAATAAAATGAGAA1880.1121979458227153No Hit
CTTACTGGGAGACCACACTTTGAATAGCAAAGCCTATGTGAATATTACTGGT1880.1121979458227153No Hit
TGATGAGGGAAGTTGGTCACCCTAAATTCATATAATATTAGTCTTCTACTCA1870.11160114823855193No Hit
TCGGGTGGGCGATCTAGTGTCGGGATCAGGAAATGTTTTCTTAAGCTTTTCC1870.11160114823855193No Hit
GTAAAGGGGGAACCTCCGAGCAACACCTAAACAAAGGGAGTTGATGCCTCCG1870.11160114823855193No Hit
TATGGCGGGCTGCAACCGGGAGGGAAGGTAAAAACAGGGACCAGCAATGTCA1860.11100435065438854No Hit
TAGGCTGGGGGCTAGATCCTTCCCATAGTATCATCTGTCCTCTGGAATGACT1860.11100435065438854No Hit
CACATTGGGTGACTCTACACATGATGTAAATATTATTTGAAGATGAACTCAA1850.11040755307022516No Hit
GAATGCGGGATTTTGGAATATTTGGACACAGTTTCCAGAAGGATGAGAGACC1850.11040755307022516No Hit
CTATCAAGGGGGGAATATGACACAAAGGTGGAATAAACATTAATTTTGTGCA1850.11040755307022516No Hit
TACTTAGGGTATGGTACTGGTGAAAGGATAGAAACATAGATCAATGGAACAC1850.11040755307022516No Hit
CTTGCTGGGGTTGAATTAGTGAATAAACAAACTAGGTCAAAAACAAATGCAG1850.11040755307022516No Hit
CAGCTAGGGTATCACAATATTTCTACTTGGTTATTAGTGATATAGAAAAGTT1850.11040755307022516No Hit
GCACAAGGGCAGTTTCTAATACAACTGACCCTTGAACAACATGGGTTTGAAC1840.1098107554860618No Hit
TTGTCAGGGGGAAATGATGGCGACTGGAACGCCAGAGTCTCAAGCGCGGTTC1840.1098107554860618No Hit
TACTGCGGGTTGATACTTACCATAGATTGAGATTTTTGCAGTTCGGGTTGCT1840.1098107554860618No Hit
TATTGCGGGGCCTCCTCCCTGTGCTCTGGGGATGCTGTTTTTTAAACACTTT1820.10861716031773505No Hit
CTGTTTGGGATAGGTAGTAATTACCAGTGTAACTAAGCATTTGTGTTCTGAT1820.10861716031773505No Hit
GCCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1820.10861716031773505No Hit
GTGCAAGGGAACCTCAAAGCTAAAAAGCCCAAGAAGGGGAAGCCCCATTGCA1810.10802036273357167No Hit
AAATGAGGGGTGAAGGCAGCCAAGAGTGAGGCTGTACCCTGAAAAGCCACAG1800.10742356514940829No Hit
GTTTAAGGGAGCGCCCCCAGGATTGAACGTGGGGAGCCCCAGGGCAGAAGCG1800.10742356514940829No Hit
AAGAATGGGTCTCTTTGGTCTGGCTGAGGGGAAGGAGGCAGAATCCCGATGT1790.10682676756524491No Hit
CTTTCGGGGTAGTCCTGAGACCCTTCGCCCCCTTTTCCTGCACCTAGTGGCC1790.10682676756524491No Hit
CTAGGCGGGCAGAGCTGATAGAGCATGGAAAAGTCTGTCCAGCGATCAGTTG1790.10682676756524491No Hit
AATGCAGGGCGTTTGATTATTCTTATTTTTAAACCATGTGCAGTATCCCTTA1780.10622996998108153No Hit
AAAAATGGGATAATGATTCATGTATTGTTCGGTTGTCTCAAAATTCTGTTTT1780.10622996998108153No Hit
AGTCATGGGGGGGAAGCAGAGTTTGAAGACGAGCCCCCTCCGCGGGAGGCTG1770.10563317239691815No Hit
CTTGCCGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG1770.10563317239691815No Hit
TAAACGCGGGATGTACTATAATGATTTTGTTAAGTTTTCCCCATTATAGGGA1770.10563317239691815No Hit
GACAGTGGGGAAAAGATTCTGGGTAATTGAGAGGCAGGTACTAGGAATAAAC1760.10503637481275477No Hit
CTCAATGTGGGCAGCCAATGGGCTATAAATTAGTGCTTCTAGAACTTTAATG1760.10503637481275477No Hit
AACGCAGGGTTAAAGGGAAATATTTTTGAAATGTTGATTGAATATTTATGAG1760.10503637481275477No Hit
CAGTTAGGGAGGTGTTTTGAGGTGTTGGCATTCTTCGCTGATTTGGCTGTTC1760.10503637481275477No Hit
CTGTACGGGGCAGTGGCACCTCGTGCAAAATAATAAACTCATCTAAGATCCT1760.10503637481275477No Hit
TCCAGTGGGGTTTTCTATGACGTATTTGGTTTTCTTCCAACCCAGTGGTGTT1760.10503637481275477No Hit
TGCCCCAGGTCCCATTGCTAGGAAACCCTTAAATTGTAGCAGCTCTTCTCTT1760.10503637481275477No Hit
GATACTGGGGATTCTAAAAGGAGCTTGGAAACCTTTCTTCTTTTTCATTTAA1750.10443957722859139No Hit
TAGCAAGGCATGGACACTGCCAAAGACACCCTGGAGACCATCGTGAGAGAAG1750.10443957722859139No Hit
TGTAGGGATTATTAGTTTTATGTGGTTTATATTTCAGGTAACATAAATTAAT1750.10443957722859139No Hit
AGACGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1750.10443957722859139No Hit
GTTGATGGGACAAACTGACTTCCAACTAATGTATAAACGATTGGGGGAAAAC1750.10443957722859139No Hit
CAACCTGGGCTACAATTTAAATTGGGATCTTCTATATTCTAGTTATTATTTG1750.10443957722859139No Hit
CGCGGGAGGGGGCTAAATGACTGGGATATGAAGTAGGAATGGGAGAGGAAAG1750.10443957722859139No Hit
TTAGTAGGGCCCGTGTCTTTGCCAGCATTTGGTGTTGTGTTTTGAATTTAGC1750.10443957722859139No Hit
AATGAATGCTTAGAAGATATAACCGTAAAAGATGGACTTTCTCTCCAGTTTA1740.10384277964442801No Hit
GCGAATGGGTGATAACGGAATGGTTGCAATTAAGGACATTTTTTTTCCTCTT1740.10384277964442801No Hit
GGCAATGGGATGTCAGATTCAAACATATTGAAATATTTTTTAGAGTATAAAA1730.10324598206026463No Hit
GCTTTGGGGTACTAACAGTGCTTTCATGAAGACTCATTATTTTTCATCTGGA1730.10324598206026463No Hit
AGAGTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1730.10324598206026463No Hit
ATGCTAGGGTAACTCATTAGACGTACTGAGGCAGCACTTCTGCAGGGTGCAT1720.10264918447610125No Hit
CAACCCGGGCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAACATCGCTGTG1720.10264918447610125No Hit
ACTGCAGGGGAGGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1720.10264918447610125No Hit
TGCTAAGGGGGAGTATTTTCATATTCCAATACCGATAAATCTTTGAGGTTTC1720.10264918447610125No Hit
GCAGCAGGGTATTTTCAGGTTTCTTTATTACAACCGTAGACTCTTTTTTACA1710.10205238689193787No Hit
GTGCGGGGGAAGGTGGTTTATTACAAATAAGCACACTAACCCCTCGGAAGTT1710.10205238689193787No Hit
TGCAATTGGGGGGGCACGCTCCAGTATGCCAGTCCTGCGGGATTACGTCCAG1710.10205238689193787No Hit
AGCTCAGGGGAGATGGAGTCTTGCTCTGTCTCCCAGGCTGCAGTGCAATGGT1710.10205238689193787No Hit
TACGCAGGGAGCTGGGTTCTAAGCTTCACCATGCAAATCAGAAATTTTATCT1710.10205238689193787No Hit
TACGAGGGGAACTCTGGAGAAAAAATAATTTGGTTGGTTGAGAATGTCTTAC1710.10205238689193787No Hit
GCGACGGGGTATCTGTTATCTTCTTGACTGTCTAACCAAAGTGATCCAAAGA1710.10205238689193787No Hit
AAGCCATGGGAGCAGACATGATCCTAGTGGATGATGATTTTCAAACCATAAT1710.10205238689193787No Hit
TTGGTGGGGACATTTATCTAGAGGAGTGATTCTCAACCTTGACTGTACATAA1700.10145558930777449No Hit
CCAGCGCGGGATTTGCCCAGCTGGTCCATGTGGTCTGTGTTCCTTTTTCTCT1700.10145558930777449No Hit
GGACATGGGCAGAATGGTGGGGCAAGTACTTGGTGCAGTCGGAGCTTTACCT1700.10145558930777449No Hit
GCATATGGGGTCTTCTAGCATCTGAGCCTCAGTAGAATCTATTCATTATTGG1700.10145558930777449No Hit
TGATGTGGGACATCACTCTTGGTCTGGGACAATAGATCAATACAGTGGGTTA1700.10145558930777449No Hit
TTGCTGGGGTCTGCATTAGGGGGGTACCATCACATAATAATCACCAATAGTT1700.10145558930777449No Hit
GCAGTTGGGCATGTGATATTTTCACATCTTAATTAAGCTCCTTCAGTTTGAA1700.10145558930777449No Hit
ATGATGGGGAACCAACTGGCAGTTTGCTAAGCATATCTACTGGTGTTGTTTC1700.10145558930777449No Hit
ACAGTGAGGGGCGCTTCTGCCCGGCCGCCCCTACTGGGAAGTGAGGAGCCCC1690.1008587917236111No Hit
CTATCAGGGATTGCAGATGCGATTCTCATTTAGCTGTCAGCAGCAGCCTGGG1690.1008587917236111No Hit
TAACCAGGGCATTTGGCAACCAAAATTACTCTAAGAATCATCACCCAAGTCA1690.1008587917236111No Hit
TTTGCAGGGCATATATATATTTGAAATAGTATAAGCTAATTACTGTTGCAGA1690.1008587917236111No Hit
ATTACAGGGCTCCCTCTGATGGTAAAAATATTGAACTTCAGACAGACACCTA1690.1008587917236111No Hit
TAACGAGGGAAGCTTCTGAAGTACAAAGATATTCCTAGAATATGACGTCATA1690.1008587917236111No Hit
GCCATGAGGAGGTTTGGCATAGAGCTGTGGTTTGCTTCCTGCATGCATTTGT1690.1008587917236111No Hit
TAATTGGGGGAAGGAAAGTAATAGCTGCTTTTAATAGGTGGTTTGAACTAGC1690.1008587917236111No Hit
TTTCGCGGGTTACTTACTATATTAAATGTGGAGAATTTGAAGATGAATCTAA1680.10026199413944772No Hit
GTTCTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1680.10026199413944772No Hit
GATACTGCATGGGAGAGAGTTCTTCCCAAACGTTGATTCAGGAATTTTCCCC1680.10026199413944772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)