FastQCFastQC Report
Sat 31 Dec 2022
SRR5845003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120555
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT5780.4794492140516776No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC5490.45539380365808135No Hit
AAGCCGGGCGATCTTGCTTAGGGGTATTTTTTTAAGTTTCAAACTCCATTCT1640.1360374932603376No Hit
TTACAAGGGAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTTAA1620.1343784994400896No Hit
AAGTGTGGGAATGGATGCACGGTGAATGCATGAATGATTGAACGACAGAAAA1620.1343784994400896No Hit
TCTAGTGGGGACAAATAGGTTCTAATTAAACTAAAGAGCTTCTGCACAGCAA1610.13354900252996557No Hit
AGCATCGGGGACGCTCTTTGATCTAGCTATGGGGTGAAACATGAGGCAAGTT1600.13271950561984158No Hit
AGGTTCGGGTATATTTAAATCCAAGAGAAACTATGCCGAAAAAAAGTGTTAT1600.13271950561984158No Hit
GGGAACGGGTATGTATGCCTATCTAATTATAAGTATCATTCACACTGTCTCC1600.13271950561984158No Hit
GATCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1580.13106051179959355No Hit
TCACGGGTCTAGAAGCGGGAGATAAGTTTTTTTCTCTTTGAAAGATAGAGAT1530.1269130272489735No Hit
CTTAAGGCGGAGTCACAATCCAGGGACTTGGGTATACTGCAAGCTGAGATTT1530.1269130272489735No Hit
CTGTGTCTGGGATTTTAAAGGTCCAGAAAGAAATTCTAATCTGTGTGCCTAT1530.1269130272489735No Hit
CTAACTGGGCCCCTGAGGTGCAGGGGAAGACCAAAGTTTGCAACTCGAACTG1500.12442453651860146No Hit
ATAAGCGGGCGATCTTGCTTAGGGATCCTAGAGAGAAATCAAAATAATTGAG1490.12359503960847745No Hit
AGGCAGGAGTACACAGAATAGAATTTAGACTGTCCCTTGAGTAGAATCCACT1490.12359503960847745No Hit
GCTGCGGGGAAATTTGGGTGTTTTTAAAACCTTGTTTGGGGTTGTTCCTGTC1490.12359503960847745No Hit
ATCCGAGGGAGAGGAGGAAGCGGCGCTCATGGGGTTGCAAGGATGTAGAGGA1490.12359503960847745No Hit
GATTTTGGGAGAGGTAAGTATAATGAATGTTTTATTGTATCTTCTTTGCAGC1460.12110654887810543No Hit
TATATTGGGGACTTTGGCAGAGACTGACTTTTTAAATCACTAGAGCCTAGCA1460.12110654887810543No Hit
GTAGCCGGGCTAGTGAAGGGATGTCAAGAAATTACATTTTGCATGTCTTATG1450.12027705196798143No Hit
GTCAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1450.12027705196798143No Hit
TTTTCAGGGACAGCTTTCTGTAGCAGAGATCACCAATGCTTGCTTTGAGCCA1450.12027705196798143No Hit
GGAGTGGGGGGTTTCAAAAAACATTTTTCCAAATGTACTATTAGAAATAAAT1450.12027705196798143No Hit
CCTAATGGGGAATCTGGCTGCTGACTTGGCACACAACTTCACTGATGGTCTG1450.12027705196798143No Hit
AAAGATGGGTCTATATGGAAAATCTCAAAGAATCAACATAAACTCATGGAAA1440.1194475550578574No Hit
TCTTTAGGGGCAGTGAGTTATTTCCCAAGTGGTAAGTAAACCTTGGAAGAGA1430.1186180581477334No Hit
CTTCAATCGGGGTGGGAATTCCCAAAGGACAGTGTGAGCAAGAAGAAGAAGT1430.1186180581477334No Hit
TAGTATGGGAAAGACCTCACAGCATACTTTGTTTTACTACGTTATTAAAAGG1410.11695906432748539No Hit
AAAACTGGGGTGAATAGAAGACATTTAGCAAATGTTTATCTATTTCTTCTTC1410.11695906432748539No Hit
GCAACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1390.11530007050723737No Hit
ATAAGTGGGGCGATGGATTCTGGACTCCACCCTCCAAGTAATCTTTGAGGTG1380.11447057359711334No Hit
TGGCATGGGGGCACCGGCCGCGCGGCGGGAGGAGGTGCCGGCCGAGCACCGC1380.11447057359711334No Hit
TCAGGAGGGATATTATAATGTAAAAATGACAAGGCAGGTACTGGGATTTTGC1370.11364107668698933No Hit
GTAACAGGGAGAAACTGGGTTCATGTTTTCAGATCCTCCAGGACAGAAAGTT1370.11364107668698933No Hit
TTTTTAGGGAAAGCAGACAAAAATAAAGAAAAATGAATGAAAGGAATGAACA1370.11364107668698933No Hit
ATACAGGGATGGAACTTGCAGAGCAATGAGAACCAAAACCATGACCCAGGAG1360.11281157977686533No Hit
GCAAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1360.11281157977686533No Hit
GCGATAGGGATACAAGCTGCAGCCTCTTTCCTCGTTTCTAGTCTCAGAAGGA1360.11281157977686533No Hit
CAGTTAGGGATACTGACCAATGTACACTATTGCTGCTTTTTAACTTTTGTGT1350.11198208286674133No Hit
AATCTTGGGTATTCAAGATGAATTTAAAAGTCAACCCTTCACACAGTTTCCC1350.11198208286674133No Hit
TTAACGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1340.11115258595661731No Hit
ATACTTGGGAGGGAAACCTGGCTCAGATAAGAATGATCCTTGCTGCATGCTC1340.11115258595661731No Hit
TCACCTGGGGGCAGAGGTGGAGGCGCTTTGAAAGCCGTCATTGTGTGGCACT1340.11115258595661731No Hit
CATAATGGGAAATGTTCTATACAAATTTGCATCACTTCTTTTCTTACAGCTT1330.11032308904649331No Hit
GCTAATGGGATTGAGTCCGGGTGATAGTTTCTCCACATTTGATACTCGTATG1320.1094935921363693No Hit
GTTATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1320.1094935921363693No Hit
TTCAGTGGGGAGAGTCGGATTCGCCGCCGCAGCAGCCGCCGCCCCCGGGAGC1320.1094935921363693No Hit
TTACTTGGGACAGTGAAAGAGTAAATTAGAAAGTGAATGGATGACTGTTCAT1320.1094935921363693No Hit
AAGAAGGGGTGATGCAAATGGGAGAGATGGAAGGCCCCTTTAGGTGGTCCAG1310.10866409522624527No Hit
AAGAGAGGGTTGCAATAACTTTCCAAATTTTCTAAATCCATGAATACCCTTT1300.10783459831612127No Hit
TGTCTCCGGGGGCAAGGTGCTCTAGGAGGTCTTCCTGGCCTCCTTGAACTGT1300.10783459831612127No Hit
GCATCCGGAAACTAATTCTTCCCTTTTTAGTCCAAGGGTCCTCTGTATTGAT1300.10783459831612127No Hit
GTTGCCGGGATATTAGTTTAAGAAAAACAAAGTATGGGTGGGAGGAAAGACA1300.10783459831612127No Hit
GCTCTTGGGCTGATTTAAAAAAACAACAACACAGGATTGAATTAAGTAGAGT1300.10783459831612127No Hit
ATCTCAGGGATATGTCTGCTGCCAGCTGCTGGTCTCCACACCCTCAACCGTT1300.10783459831612127No Hit
GGTGTTGGGACTCGTAGTCAGGCGGCGGCGCAGGCGGCGGCGGCGGCATAGC1290.10700510140599725No Hit
CTACGTGGGGGATGAAAAGTTTAATCCAACAAGAAGATATAACAATTTAAAC1290.10700510140599725No Hit
CTCCCCGGGGTCTGCCTATGCAAATTTCTATAGCATTTGGGGCCCAACCTAA1290.10700510140599725No Hit
GTCAGCGGGGTCAGCAGCCATTGCCACGAAGATGGCGCAATTTCATGGCATG1290.10700510140599725No Hit
CCCTAGGGGACGCTCTTCCGATCTCGTCAAGGGACCATTGCCCCCTTCGTGA1280.10617560449587325No Hit
ATCAATGGGTGTACAGGTCAGTAGCTGCATTAAGTACATTCACATTATTGTG1280.10617560449587325No Hit
TAACTTGGGCAGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGCAATTCTCC1270.10534610758574925No Hit
GCCCTGGGGCACAAGTGGCATTGATACTTTTAACATGCGTTGTGGAAATTAG1270.10534610758574925No Hit
TCATACGGCAAGATTTTGAGTATATAAATAGAAATAAAATACATTAACTAAA1270.10534610758574925No Hit
ACAGCGGGAGGCTTGAGCCACCAGGATATGGGTTTTTGAGATGATGAAAATG1270.10534610758574925No Hit
TATCAGGGTCACTCTGTAATTCTCAAAGACTATTTTCAAAATTTGGTCAAAT1260.10451661067562525No Hit
ATATCGGGCTCAGGAATGTTGTCTGAATACTTTTCAGTATGAAAATACAAGA1260.10451661067562525No Hit
GCGCATGGGTGGGCACTGGGATATGTTACAGGTGTGGGTCCACAGAGCACGA1260.10451661067562525No Hit
GGCACTGGGGTGTAGGTATTATATGACAGGAAAGAATCACATCTTAAGATTT1260.10451661067562525No Hit
TATTGGGGGTCCACTAATTTTTTTATTTTGATATTAGTCTTTTTAAAAAAAT1260.10451661067562525No Hit
ACATAGGGGAGCCCCGGATGGTCAGTTAAGAGCTGTTAACCACAGACCTGAG1260.10451661067562525No Hit
ATTATTGGGAAGTGTGAGTTGCTTGATTTCTTTCTGCTATGCTTCAGCTCAG1250.10368711376550122No Hit
ACTGCTGGGTGGTCGTCCCAGTAACTTTTGCTCCCATTTTCTACAAGTATTT1250.10368711376550122No Hit
GCACAGGGGGGAAAATAAAGAAGAAATGGTCAAATTCCTAGACACATACAAC1250.10368711376550122No Hit
TGCATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1240.10285761685537721No Hit
GGATTTGGGGAGATCAGTCTGTAAAATGGTTTCACAAGAATGTGAAACTATT1240.10285761685537721No Hit
GTGCTTGGGAATCTTAAGAAATTATCAAGTTGGAACCAAGAAATAGCAAGGA1230.1020281199452532No Hit
AATGAGGGGTATTTTAAGATCCTTACATTATGAATGAAGCAGTATATTATTT1230.1020281199452532No Hit
TATTTTGGGAGGCCACTTCTGACCTAGGATAAGGATGTGGCTGAAGAATAGA1230.1020281199452532No Hit
GATAACGGGCACCTGCCTTTTCACCGATAGAAGCTGGGATAGGATTTGCTTT1220.10119862303512919No Hit
TAGCCTGATTTCCTTCTTTCTGTATCTGTTGTCTTCTCTCCTCAGCTCTTTG1220.10119862303512919No Hit
TATAGGGGGACTCTCTCAAAACGTTACTGAAAATCAAAATCCTAAAACTCAT1220.10119862303512919No Hit
ATGCAGGGGCGGCGGCGGAAGTGGCCGGCGAGCCCGGTCCCCGCCGGCACCA1210.10036912612500519No Hit
ACACATTCAATGCAATTCCCATCAAAATTCACTATTCTTCACAGAATTAGAA1210.10036912612500519No Hit
TGACATGGGCTTGTAGATGTTTTTACAAACTCACATTTGAAATCTAAATAAG1210.10036912612500519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)