FastQCFastQC Report
Sat 31 Dec 2022
SRR5844982.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844982.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23518
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT3081.3096351730589337No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC2891.2288459903052982No Hit
GTAGGCGGGGCTTCTAGCATCCTTCATCCTTCAGGTACCAGCCATCCAGACA2431.0332511267964963No Hit
GCTGAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCC2060.8759248235394167No Hit
GACTGGGGAGACAGAAAGATGCAGGACAGCATTTCATATGTAACCATTTGAA1980.841908325537886No Hit
GTATGTAGGGGCAACTGTTGGAATTCACTTTATTGTAGAAAAACCCAAACTG1960.8334042010375032No Hit
TTCTACGGGCTGGAATATCTGGCTTGATAGTTTTCTCCTTTTCAACATTTTG1930.8206480142869291No Hit
GACTCAGGGGAGAAACACCACTTCCAGGAAGATTTTTAGAATGTATTACAAG1930.8206480142869291No Hit
TATCGTGGGTGTGTGCCCAAGGGAGGGCCCCCCAGGGTGGCCCCCAACCCGA1890.8036397652861639No Hit
TCAAAAGGGAAGATACTTGATGCACTTATTTTTTAATGTGAGACAGCAAGTT1800.7653712050344417No Hit
CGTATAGGGACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT1790.7611191427842503No Hit
TTTCGAGGGACCTGCCGTCTAGAAAAACCTGCCAAATATGATGACATCAAGA1720.731354707032911No Hit
CACTGCGGGAGCTGATGGTGAGCTGACAGTGTCCACAGGTTCCAGGACCATT1670.7100943957819542No Hit
TAATGCTGGGGAGACCCCATCTCCATTCTCACCCAGTTCCTCTCTCCTTCCC1650.7015902712815716No Hit
TCCTGGGGGCTGTTCCTTGTACTCTTAGGATATTTGAGTCACTTGTGTCAAC1630.6930861467811888No Hit
GCGTCAGGGAGGGTAGCGCGCGCCTGTCTCTCTTCGGGTCTCGGGCCCTTGG1600.6803299600306149No Hit
TGCTATGGGTGTCAAGATGCTACAATGGAAACCATTTCTGGTTTTATCTTCA1600.6803299600306149No Hit
TGCTAAGGGGAGGACCCCAGGTTCAAAAGTAAAAGCAGTGGCCCTGCTTTGG1560.6633217110298495No Hit
TAACGGGAGCAGTACATATGTGATACTCAGTTTTGTAGACTGCAGACCATAA1530.6505655242792755No Hit
TTGTCCAGGGAGGCGTCCGCGTCGCTAGCTAGTCGTTCTGAAGCGGCGGCCA1510.6420613997788928No Hit
AGACTGGGGGTTCTGCAAGACGCTGTCCTCAGGGACCATTAGGGGACCATTC1490.6335572752785101No Hit
GGAGTTGGGCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAAGTAAAA1470.6250531507781274No Hit
AGTCCAGGGTCACCTGCAGACCTCTTCAAGTGACACTATGTAGGAATCCTTC1460.620801088527936No Hit
CTTCTGGGGAAGATAAAGAAAGCAATGATTCGGAAGTCCAGTAGGGCCCATT1420.6037928395271707No Hit
AGCCCGGGGGAGCTGACTCCCGTAGAGGAAGACACTGTGGAGGCCAGTTCTG1420.6037928395271707No Hit
TGAATGGGGGAGGCGAAGAACGCAAAGCTGAGAACATGGACGTTAATATCGC1410.5995407772769793No Hit
TGCTGGGGTGTGACTAAAAGGGAGGCAAATTATTGGAACGGATTATTCAAAT1390.5910366527765967No Hit
TTCACTGGGGAGGCTTAATTGGCTTATGGTTCTATAGGCTGTACAAGCAGCA1320.5612722170252572No Hit
CTGCAGGGGAACCAAGGTGAAAAACAAAAGCAAGAAAAAGAAGCCAAAGGAT1320.5612722170252572No Hit
TATAGAGGGGAAATGAAATTGTATGTGTAAAAATGTACGATTTTTCACTGTG1280.5442639680244918No Hit
GTGACTGGGATTCCCTGGTCACCCCAGCCGGCAGTTGCCCAAGATCTGATAC1180.5017433455225784No Hit
AAACATGGGCTTTGGGGAGTCAGATGAGCTGATTGGGCAGAAAGTGGCCCAT1170.4974912832723871No Hit
GGGTTTCTGGGCACCCTCGAAGTTTTCTGGATGTCTTTTTATCTTTCTCGTG1110.4719789097712391No Hit
GCACCGGGGCTGTGGCCAGGCCTGCAGACAGACCTCAAGGGACAGGGAATGC1110.4719789097712391No Hit
AATATTGGGTAAGTGCAAGCAGTCACGTGAAATGAGCCTGATAGTCTGCGAA1040.4422144740198996No Hit
CATAATGGGATAAATATATATATATAAAATATTCAGCAGCACCAAGTTTTAT1030.43796241176970835No Hit
TTGTGAGGGACAGCGGACACCAGGACTCCAAAATGGCGTCAGTTGTACCAGT1020.433710349519517No Hit
GTGTGGGGGAAGGTTGGGAAACTTATATTCTCCTTCAAATTAGGATTCTAGC1020.433710349519517No Hit
TACAGAAGGGAGATCCTTTGATTTTAGAAGAAAATGATGTAGACAATATGGC980.4167021005187516No Hit
TTACGGGGGAAGAAAATACATGTGGGATCTTGCCAAGGTGCTGCCTTTAGGA970.41245003826856025No Hit
GACCATGGGGGCCTCTGCCGCCGCGGAGCTTCCCGAACCTCTTCAGCCGCCC950.4039459137681775No Hit
TAAGCAGGGGAAATGAACTGGAACCCTGATAAACTGTAAATTGAAATGATAA930.3954417892677949No Hit
GCCCAGCCTCTGAGTTTTCTGTTCTATTTTTTTTTTAACCCCAATTATCCTT930.3954417892677949No Hit
CTTCATGGCTCCTATGACATCGTGCTGGAGAAGGACGAGACTCTGGATGTGG930.3954417892677949No Hit
TGTACTGGGGAGCATAAAGAACCGACGGGAATCTGGGCTTTCCCACTCTGGA920.3911897270176035No Hit
TAAGGCGGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCT920.3911897270176035No Hit
CAAGATGGGCTAGAAATGGCTGCAAGTGGAAGCGGATAATATGTAGGCACTT910.3869376647674122No Hit
GCGGGCGGGCCAGCCTTTTTTTTGAAATTTCTCAAACTCATTTACTAGTTCT860.3656773535164555No Hit
TTTATGGGGGATCTGTCGAGGGGGGCCCGAAACTGATGATCCCGCCTGCGTC850.3614252912662641No Hit
GTCATGGGGAGAAGGAGGGGATCAAAGATAGCTGATCTCACATGCTTCCCAG840.3571732290160728No Hit
TACTGGGACTAAAGTGAGATGCATTTTTTCTCATTTTCAGCAAGACTCCTCT830.3529211667658815No Hit
ATGTCAGGGGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAA800.3401649800153074No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAG780.33166085551492475No Hit
AGCTATGGGAGGATGGCTGTGCTGTATCCTCAAGTTATTGTCGACCATCCAT770.32740879326473343No Hit
AGACTGGGGGAAACATATTGAAAATCAACGCAGATTCAATAGAACCTGCAGA740.3146526065141594No Hit
TCAAGGCCGGGATTGCCGAGAACTCCACAAATGCTCCCTATGTGCTCATCGG680.28914023301301134No Hit
AGTCTCGGGGCCACCCTCCCTGTAAGTCTATTTAAAAACATCGACGATACAT680.28914023301301134No Hit
ATAGATGGGAGCGTTTCTTGTCTCAGCCATTCCAGGTTGCTGAGGTCTTCAC650.2763840462624373No Hit
GCAATTGGGATGGAATCTGGGCTGTACTTGAGTATGGAAATTCTCTTATAGA640.2721319840122459No Hit
GCGCGGGGGGGAGCCTTCTGCGAAGTGACTGGCAGCGCTGGCCCAGTGTTAT570.24236754826090653No Hit
TTATCCTGGGTGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGT500.21260311250956712No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGA500.21260311250956712No Hit
CCCGCTGCTTGCCACCCCCAACCCTGCCCTCTGGCCCACACAGAAAGGTGGC500.21260311250956712No Hit
ATACAAGGGACAGCAGGAGGTGAGTGGCAGTGGTGAGCAAGCATTACCACCT500.21260311250956712No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCCTGTCTCT490.2083510502593758No Hit
AAAGCTGGGCAGATGGCAGAGTGACCGCGCATACCTGCTTCCAAGAATAAAA490.2083510502593758No Hit
TCAGGCGGGAGCGCCGGACTGTGGTGGACAGTGAGGGCCGGACAGAGACTAC470.19984692575899313No Hit
GCTGATGGGGAGGCTCGGCCGCCTGAGCCGCGGACGGTTTGCTGAGCCCGTT460.19559486350880176No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGACTGTCTCTTATACAC460.19559486350880176No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCCTGTCTCTTATAC440.18709073900841908No Hit
CGAAAAGGGGATCTTTTCTCTTCCAATCAAATGTCTAGGCTTGTTTGACTTC410.17433455225784505No Hit
TTCACTGGGACTTCAGCGATTCCATTGTGATAAGCGCACAAACAGCACTGTC380.16157836550727103No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGACTGTCTCTTA380.16157836550727103No Hit
CACGGGCGGGAGTCTGTAACTGAGCAAGGAGCTGAATTATCCAATGAGGAGA380.16157836550727103No Hit
GAACCAGGGATGTTGGAAATCTAAGAGTCTTTGGGATATTACGTTATAGTAA360.15307424100688835No Hit
ACTGAAGGGACCCAGTTGCTTCAGCGAGTCGAACTACAGTTTTAACCTCATC360.15307424100688835No Hit
GCTTCTGGGAGCAAACTGCTTTTCCATTAATGGAGAATTCATGCCTTTCAAG350.148822178756697No Hit
TGGTGTGGGCAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATC340.14457011650650567No Hit
ATGCTGGGAGAGTGAGACCCTGTCTTGAAAATAATAATAGGCTGGTCACAGT340.14457011650650567No Hit
ACCATTGGGACACAGGATGAATTTGAACCTTTGGTCTCATTTATGGAAAAAC320.13606599200612296No Hit
TGCTAAGGGAGCTATGACTTGAAACAGAGTCTGTATGGTTAATATTTTTCCT320.13606599200612296No Hit
GATCAAGGGAGGAACAGTGCAGAAACTAGAATCAAGCCTTATAATATCTTAT300.12756186750574028No Hit
GCCAAAGGGGGGGGGCGGGAGGAGCAAACGCAGGAGGCCATGGCCGCCGCCG300.12756186750574028No Hit
TGCAGTAGGGCTGTGAAGTGAGACCTTCCTTTTACTTTTCTTCTATTGCCTC280.1190577430053576No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGAACTCGTATGC260.1105536185049749No Hit
GGTACAGGGCTTTCACATTCGGGAAGCGTCGGGATTAGGTGAAAGAAGCTGA250.10630155625478356No Hit
TATGCCGGGTTGACTTGAAGATTACAAAATTTAAGGTTTTTTGGCATGTGTG240.10204949400459222No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)