FastQCFastQC Report
Sat 31 Dec 2022
SRR5844981.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844981.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23881
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT3221.3483522465558393No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC2891.2101670784305514No Hit
GTAGGCGGGGCTTCTAGCATCCTTCATCCTTCAGGTACCAGCCATCCAGACA2481.038482475608224No Hit
CGTATAGGGACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT2401.0049830409111846No Hit
GCGTCAGGGAGGGTAGCGCGCGCCTGTCTCTCTTCGGGTCTCGGGCCCTTGG2040.854235584774507No Hit
GTATGTAGGGGCAACTGTTGGAATTCACTTTATTGTAGAAAAACCCAAACTG2040.854235584774507No Hit
GCTGAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCC2000.8374858674259873No Hit
TTCTACGGGCTGGAATATCTGGCTTGATAGTTTTCTCCTTTTCAACATTTTG1960.8207361500774675No Hit
GGAGTTGGGCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAAGTAAAA1940.8123612914032077No Hit
GACTCAGGGGAGAAACACCACTTCCAGGAAGATTTTTAGAATGTATTACAAG1900.7956115740546879No Hit
TTTCGAGGGACCTGCCGTCTAGAAAAACCTGCCAAATATGATGACATCAAGA1870.7830492860432979No Hit
GACTGGGGAGACAGAAAGATGCAGGACAGCATTTCATATGTAACCATTTGAA1850.7746744273690381No Hit
CACTGCGGGAGCTGATGGTGAGCTGACAGTGTCCACAGGTTCCAGGACCATT1800.7537372806833885No Hit
TGCTATGGGTGTCAAGATGCTACAATGGAAACCATTTCTGGTTTTATCTTCA1780.7453624220091286No Hit
AGACTGGGGGTTCTGCAAGACGCTGTCCTCAGGGACCATTAGGGGACCATTC1750.7328001339977388No Hit
TCAAAAGGGAAGATACTTGATGCACTTATTTTTTAATGTGAGACAGCAAGTT1730.7244252753234789No Hit
TATCGTGGGTGTGTGCCCAAGGGAGGGCCCCCCAGGGTGGCCCCCAACCCGA1570.6574264059293999No Hit
TCCTGGGGGCTGTTCCTTGTACTCTTAGGATATTTGAGTCACTTGTGTCAAC1530.6406766885808801No Hit
TAACGGGAGCAGTACATATGTGATACTCAGTTTTGTAGACTGCAGACCATAA1470.6155521125581005No Hit
AGCCCGGGGGAGCTGACTCCCGTAGAGGAAGACACTGTGGAGGCCAGTTCTG1460.6113646832209707No Hit
TAATGCTGGGGAGACCCCATCTCCATTCTCACCCAGTTCCTCTCTCCTTCCC1450.6071772538838407No Hit
TGCTAAGGGGAGGACCCCAGGTTCAAAAGTAAAAGCAGTGGCCCTGCTTTGG1380.5778652485239312No Hit
AGTCCAGGGTCACCTGCAGACCTCTTCAAGTGACACTATGTAGGAATCCTTC1330.5569281018382815No Hit
TTCACTGGGGAGGCTTAATTGGCTTATGGTTCTATAGGCTGTACAAGCAGCA1330.5569281018382815No Hit
TGCTGGGGTGTGACTAAAAGGGAGGCAAATTATTGGAACGGATTATTCAAAT1320.5527406725011516No Hit
TATAGAGGGGAAATGAAATTGTATGTGTAAAAATGTACGATTTTTCACTGTG1230.5150538084669821No Hit
GACCATGGGGGCCTCTGCCGCCGCGGAGCTTCCCGAACCTCTTCAGCCGCCC1220.5108663791298522No Hit
TTACGGGGGAAGAAAATACATGTGGGATCTTGCCAAGGTGCTGCCTTTAGGA1210.5066789497927222No Hit
TTGTCCAGGGAGGCGTCCGCGTCGCTAGCTAGTCGTTCTGAAGCGGCGGCCA1200.5024915204555923No Hit
CTTCTGGGGAAGATAAAGAAAGCAATGATTCGGAAGTCCAGTAGGGCCCATT1170.4899292324442025No Hit
TGAATGGGGGAGGCGAAGAACGCAAAGCTGAGAACATGGACGTTAATATCGC1170.4899292324442025No Hit
AAACATGGGCTTTGGGGAGTCAGATGAGCTGATTGGGCAGAAAGTGGCCCAT1160.48574180310707255No Hit
CTGCAGGGGAACCAAGGTGAAAAACAAAAGCAAGAAAAAGAAGCCAAAGGAT1160.48574180310707255No Hit
GGGTTTCTGGGCACCCTCGAAGTTTTCTGGATGTCTTTTTATCTTTCTCGTG1120.4689920857585528No Hit
GTGACTGGGATTCCCTGGTCACCCCAGCCGGCAGTTGCCCAAGATCTGATAC1100.4606172270842929No Hit
GCACCGGGGCTGTGGCCAGGCCTGCAGACAGACCTCAAGGGACAGGGAATGC1090.45642979774716297No Hit
CTTCATGGCTCCTATGACATCGTGCTGGAGAAGGACGAGACTCTGGATGTGG1030.43130522172438346No Hit
AATATTGGGTAAGTGCAAGCAGTCACGTGAAATGAGCCTGATAGTCTGCGAA1000.41874293371299365No Hit
GTGTGGGGGAAGGTTGGGAAACTTATATTCTCCTTCAAATTAGGATTCTAGC1000.41874293371299365No Hit
GCGGGCGGGCCAGCCTTTTTTTTGAAATTTCTCAAACTCATTTACTAGTTCT980.41036807503873374No Hit
GCCCAGCCTCTGAGTTTTCTGTTCTATTTTTTTTTTAACCCCAATTATCCTT970.40618064570160384No Hit
TCAAGGCCGGGATTGCCGAGAACTCCACAAATGCTCCCTATGTGCTCATCGG960.4019932163644739No Hit
TTTATGGGGGATCTGTCGAGGGGGGCCCGAAACTGATGATCCCGCCTGCGTC950.3978057870273439No Hit
AGCTATGGGAGGATGGCTGTGCTGTATCCTCAAGTTATTGTCGACCATCCAT940.393618357690214No Hit
AGACTGGGGGAAACATATTGAAAATCAACGCAGATTCAATAGAACCTGCAGA920.3852434990159541No Hit
ATGTCAGGGGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAA890.3726812110045643No Hit
TTGTGAGGGACAGCGGACACCAGGACTCCAAAATGGCGTCAGTTGTACCAGT880.36849378166743435No Hit
TAAGCAGGGGAAATGAACTGGAACCCTGATAAACTGTAAATTGAAATGATAA880.36849378166743435No Hit
TGTACTGGGGAGCATAAAGAACCGACGGGAATCTGGGCTTTCCCACTCTGGA870.36430635233030445No Hit
TACTGGGACTAAAGTGAGATGCATTTTTTCTCATTTTCAGCAAGACTCCTCT860.3601189229931745No Hit
GTCATGGGGAGAAGGAGGGGATCAAAGATAGCTGATCTCACATGCTTCCCAG820.34336920564465473No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAG810.33918177630752483No Hit
TAAGGCGGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCT790.3308069176332649No Hit
CAAGATGGGCTAGAAATGGCTGCAAGTGGAAGCGGATAATATGTAGGCACTT770.32243205895900506No Hit
GAACCAGGGATGTTGGAAATCTAAGAGTCTTTGGGATATTACGTTATAGTAA740.30986977094761525No Hit
TACAGAAGGGAGATCCTTTGATTTTAGAAGAAAATGATGTAGACAATATGGC740.30986977094761525No Hit
AGTCTCGGGGCCACCCTCCCTGTAAGTCTATTTAAAAACATCGACGATACAT680.28474519492483563No Hit
CATAATGGGATAAATATATATATATAAAATATTCAGCAGCACCAAGTTTTAT660.2763703362505758No Hit
GCGCGGGGGGGAGCCTTCTGCGAAGTGACTGGCAGCGCTGGCCCAGTGTTAT640.2679954775763159No Hit
GCAATTGGGATGGAATCTGGGCTGTACTTGAGTATGGAAATTCTCTTATAGA630.26380804823918597No Hit
ATAGATGGGAGCGTTTCTTGTCTCAGCCATTCCAGGTTGCTGAGGTCTTCAC580.24287090155353627No Hit
TCAGGCGGGAGCGCCGGACTGTGGTGGACAGTGAGGGCCGGACAGAGACTAC570.23868347221640634No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGA570.23868347221640634No Hit
TTCACTGGGACTTCAGCGATTCCATTGTGATAAGCGCACAAACAGCACTGTC530.22193375486788658No Hit
CGAAAAGGGGATCTTTTCTCTTCCAATCAAATGTCTAGGCTTGTTTGACTTC530.22193375486788658No Hit
ATACAAGGGACAGCAGGAGGTGAGTGGCAGTGGTGAGCAAGCATTACCACCT530.22193375486788658No Hit
AAAGCTGGGCAGATGGCAGAGTGACCGCGCATACCTGCTTCCAAGAATAAAA520.21774632553075668No Hit
GCCAAAGGGGGGGGGCGGGAGGAGCAAACGCAGGAGGCCATGGCCGCCGCCG500.20937146685649682No Hit
GCTGATGGGGAGGCTCGGCCGCCTGAGCCGCGGACGGTTTGCTGAGCCCGTT490.20518403751936687No Hit
CCCGCTGCTTGCCACCCCCAACCCTGCCCTCTGGCCCACACAGAAAGGTGGC440.18424689083371718No Hit
GGTACAGGGCTTTCACATTCGGGAAGCGTCGGGATTAGGTGAAAGAAGCTGA400.16749717348519744No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGACTGTCTCTTA390.16330974414806748No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCCTGTCTCT380.15912231481093755No Hit
TGCTAAGGGAGCTATGACTTGAAACAGAGTCTGTATGGTTAATATTTTTCCT370.15493488547380763No Hit
TGGTGTGGGCAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATC350.14656002679954777No Hit
TTATCCTGGGTGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGT330.1381851681252879No Hit
CACGGGCGGGAGTCTGTAACTGAGCAAGGAGCTGAATTATCCAATGAGGAGA330.1381851681252879No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCCTGTCTCTTATAC330.1381851681252879No Hit
GCTTCTGGGAGCAAACTGCTTTTCCATTAATGGAGAATTCATGCCTTTCAAG310.129810309451028No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGAT280.1172480214396382No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGAACTCGTATGC270.11306059210250827No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGACTGTCTCTTATACAC270.11306059210250827No Hit
AAAGCTGGGGAAATACCTGTTATACTAAAATTATGATACATAATATCTGAGC260.10887316276537834No Hit
ACCATTGGGACACAGGATGAATTTGAACCTTTGGTCTCATTTATGGAAAAAC260.10887316276537834No Hit
ACTGAAGGGACCCAGTTGCTTCAGCGAGTCGAACTACAGTTTTAACCTCATC240.10049830409111847No Hit
ATGCTGGGAGAGTGAGACCCTGTCTTGAAAATAATAATAGGCTGGTCACAGT240.10049830409111847No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)