FastQCFastQC Report
Sat 31 Dec 2022
SRR5844980.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844980.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23446
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGC3121.3307173931587477No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCTCGTATGCCGTCT3051.3008615542096733No Hit
GTAGGCGGGGCTTCTAGCATCCTTCATCCTTCAGGTACCAGCCATCCAGACA2290.9767124456197219No Hit
GCTGAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCC2220.9468566066706475No Hit
TTCTACGGGCTGGAATATCTGGCTTGATAGTTTTCTCCTTTTCAACATTTTG2200.9383263669709119No Hit
GACTCAGGGGAGAAACACCACTTCCAGGAAGATTTTTAGAATGTATTACAAG2160.9212658875714408No Hit
CGTATAGGGACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT2150.917000767721573No Hit
GTATGTAGGGGCAACTGTTGGAATTCACTTTATTGTAGAAAAACCCAAACTG2090.8914100486223663No Hit
CACTGCGGGAGCTGATGGTGAGCTGACAGTGTCCACAGGTTCCAGGACCATT1790.7634564531263328No Hit
AGCCCGGGGGAGCTGACTCCCGTAGAGGAAGACACTGTGGAGGCCAGTTCTG1730.7378657340271262No Hit
TTTCGAGGGACCTGCCGTCTAGAAAAACCTGCCAAATATGATGACATCAAGA1690.720805254627655No Hit
GACTGGGGAGACAGAAAGATGCAGGACAGCATTTCATATGTAACCATTTGAA1650.7037447752281839No Hit
TCAAAAGGGAAGATACTTGATGCACTTATTTTTTAATGTGAGACAGCAAGTT1610.6866842958287128No Hit
TATCGTGGGTGTGTGCCCAAGGGAGGGCCCCCCAGGGTGGCCCCCAACCCGA1560.6653586965793739No Hit
TCCTGGGGGCTGTTCCTTGTACTCTTAGGATATTTGAGTCACTTGTGTCAAC1530.6525633370297705No Hit
TAACGGGAGCAGTACATATGTGATACTCAGTTTTGTAGACTGCAGACCATAA1460.622707498080696No Hit
TAATGCTGGGGAGACCCCATCTCCATTCTCACCCAGTTCCTCTCTCCTTCCC1440.6141772583809605No Hit
GGAGTTGGGCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAAGTAAAA1420.605647018681225No Hit
AGACTGGGGGTTCTGCAAGACGCTGTCCTCAGGGACCATTAGGGGACCATTC1400.5971167789814893No Hit
TGCTATGGGTGTCAAGATGCTACAATGGAAACCATTTCTGGTTTTATCTTCA1390.5928516591316215No Hit
GTGACTGGGATTCCCTGGTCACCCCAGCCGGCAGTTGCCCAAGATCTGATAC1360.5800562995820183No Hit
TTCACTGGGGAGGCTTAATTGGCTTATGGTTCTATAGGCTGTACAAGCAGCA1360.5800562995820183No Hit
GCGTCAGGGAGGGTAGCGCGCGCCTGTCTCTCTTCGGGTCTCGGGCCCTTGG1330.5672609400324149No Hit
TGAATGGGGGAGGCGAAGAACGCAAAGCTGAGAACATGGACGTTAATATCGC1320.5629958201825471No Hit
GGGTTTCTGGGCACCCTCGAAGTTTTCTGGATGTCTTTTTATCTTTCTCGTG1280.5459353407830759No Hit
TTGTCCAGGGAGGCGTCCGCGTCGCTAGCTAGTCGTTCTGAAGCGGCGGCCA1270.5416702209332082No Hit
TATAGAGGGGAAATGAAATTGTATGTGTAAAAATGTACGATTTTTCACTGTG1230.5246097415337371No Hit
TGCTAAGGGGAGGACCCCAGGTTCAAAAGTAAAAGCAGTGGCCCTGCTTTGG1170.4990190224345304No Hit
TTGTGAGGGACAGCGGACACCAGGACTCCAAAATGGCGTCAGTTGTACCAGT1150.4904887827347948No Hit
TGCTGGGGTGTGACTAAAAGGGAGGCAAATTATTGGAACGGATTATTCAAAT1140.48622366288492713No Hit
AGTCCAGGGTCACCTGCAGACCTCTTCAAGTGACACTATGTAGGAATCCTTC1130.4819585430350593No Hit
TGTACTGGGGAGCATAAAGAACCGACGGGAATCTGGGCTTTCCCACTCTGGA1090.46489806363558817No Hit
GTGTGGGGGAAGGTTGGGAAACTTATATTCTCCTTCAAATTAGGATTCTAGC1070.45636782393585257No Hit
CTTCATGGCTCCTATGACATCGTGCTGGAGAAGGACGAGACTCTGGATGTGG1050.447837584236117No Hit
CTTCTGGGGAAGATAAAGAAAGCAATGATTCGGAAGTCCAGTAGGGCCCATT1040.4435724643862492No Hit
GCCCAGCCTCTGAGTTTTCTGTTCTATTTTTTTTTTAACCCCAATTATCCTT1010.43077710483664594No Hit
AAACATGGGCTTTGGGGAGTCAGATGAGCTGATTGGGCAGAAAGTGGCCCAT1000.42651198498677817No Hit
GCGGGCGGGCCAGCCTTTTTTTTGAAATTTCTCAAACTCATTTACTAGTTCT980.41798174528704257No Hit
TAAGCAGGGGAAATGAACTGGAACCCTGATAAACTGTAAATTGAAATGATAA960.40945150558730703No Hit
CTGCAGGGGAACCAAGGTGAAAAACAAAAGCAAGAAAAAGAAGCCAAAGGAT950.4051863857374392No Hit
GCACCGGGGCTGTGGCCAGGCCTGCAGACAGACCTCAAGGGACAGGGAATGC950.4051863857374392No Hit
GACCATGGGGGCCTCTGCCGCCGCGGAGCTTCCCGAACCTCTTCAGCCGCCC940.40092126588757143No Hit
AGACTGGGGGAAACATATTGAAAATCAACGCAGATTCAATAGAACCTGCAGA870.371065426938497No Hit
TTACGGGGGAAGAAAATACATGTGGGATCTTGCCAAGGTGCTGCCTTTAGGA850.36253518723876144No Hit
TACTGGGACTAAAGTGAGATGCATTTTTTCTCATTTTCAGCAAGACTCCTCT840.3582700673888936No Hit
CAAGATGGGCTAGAAATGGCTGCAAGTGGAAGCGGATAATATGTAGGCACTT840.3582700673888936No Hit
AATATTGGGTAAGTGCAAGCAGTCACGTGAAATGAGCCTGATAGTCTGCGAA820.34973982768915807No Hit
TTTATGGGGGATCTGTCGAGGGGGGCCCGAAACTGATGATCCCGCCTGCGTC810.3454747078392903No Hit
TACAGAAGGGAGATCCTTTGATTTTAGAAGAAAATGATGTAGACAATATGGC800.3412095879894225No Hit
ATGTCAGGGGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAA790.33694446813955475No Hit
TCAAGGCCGGGATTGCCGAGAACTCCACAAATGCTCCCTATGTGCTCATCGG750.3198839887400836No Hit
CATAATGGGATAAATATATATATATAAAATATTCAGCAGCACCAAGTTTTAT750.3198839887400836No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAG690.29429326964087693No Hit
AGTCTCGGGGCCACCCTCCCTGTAAGTCTATTTAAAAACATCGACGATACAT690.29429326964087693No Hit
AGCTATGGGAGGATGGCTGTGCTGTATCCTCAAGTTATTGTCGACCATCCAT680.29002814979100916No Hit
GCAATTGGGATGGAATCTGGGCTGTACTTGAGTATGGAAATTCTCTTATAGA680.29002814979100916No Hit
ATACAAGGGACAGCAGGAGGTGAGTGGCAGTGGTGAGCAAGCATTACCACCT670.28576302994114133No Hit
ATAGATGGGAGCGTTTCTTGTCTCAGCCATTCCAGGTTGCTGAGGTCTTCAC620.2644374306918025No Hit
TTCACTGGGACTTCAGCGATTCCATTGTGATAAGCGCACAAACAGCACTGTC610.26017231084193465No Hit
GCGCGGGGGGGAGCCTTCTGCGAAGTGACTGGCAGCGCTGGCCCAGTGTTAT610.26017231084193465No Hit
AAAGCTGGGCAGATGGCAGAGTGACCGCGCATACCTGCTTCCAAGAATAAAA600.2559071909920669No Hit
GTCATGGGGAGAAGGAGGGGATCAAAGATAGCTGATCTCACATGCTTCCCAG590.2516420711421991No Hit
GAACCAGGGATGTTGGAAATCTAAGAGTCTTTGGGATATTACGTTATAGTAA580.2473769512923313No Hit
TAAGGCGGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCT560.23884671159259577No Hit
TCAGGCGGGAGCGCCGGACTGTGGTGGACAGTGAGGGCCGGACAGAGACTAC550.23458159174272797No Hit
CCCGCTGCTTGCCACCCCCAACCCTGCCCTCTGGCCCACACAGAAAGGTGGC540.2303164718928602No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCCTGTCTCT530.2260513520429924No Hit
TTATCCTGGGTGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGT520.2217862321931246No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGATCCTGTCTCTTATAC510.21752111234325686No Hit
GCTGATGGGGAGGCTCGGCCGCCTGAGCCGCGGACGGTTTGCTGAGCCCGTT500.21325599249338908No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGA500.21325599249338908No Hit
CGAAAAGGGGATCTTTTCTCTTCCAATCAAATGTCTAGGCTTGTTTGACTTC460.19619551309391792No Hit
GCTTCTGGGAGCAAACTGCTTTTCCATTAATGGAGAATTCATGCCTTTCAAG430.1834001535443146No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGAACTCGTATGC410.17486991384457903No Hit
GCCAAAGGGGGGGGGCGGGAGGAGCAAACGCAGGAGGCCATGGCCGCCGCCG410.17486991384457903No Hit
ACCATTGGGACACAGGATGAATTTGAACCTTTGGTCTCATTTATGGAAAAAC410.17486991384457903No Hit
ACTGAAGGGACCCAGTTGCTTCAGCGAGTCGAACTACAGTTTTAACCTCATC400.17060479399471126No Hit
TGGTGTGGGCAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATC390.16633967414484346No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCGTAGACTGTCTCTTATACAC330.14074895504563678No Hit
GGTACAGGGCTTTCACATTCGGGAAGCGTCGGGATTAGGTGAAAGAAGCTGA280.11942335579629788No Hit
CATTTTGGGGGGAACCTTCCAACATCTGGGAACATTTCAGGGTTTATACGAA280.11942335579629788No Hit
AAAGCTGGGGAAATACCTGTTATACTAAAATTATGATACATAATATCTGAGC270.1151582359464301No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCGTAGACTGTCTCTTA270.1151582359464301No Hit
CACGGGCGGGAGTCTGTAACTGAGCAAGGAGCTGAATTATCCAATGAGGAGA270.1151582359464301No Hit
TTGTAGGGGGGGCGCTGGTGCTTGAGCTGTGCTGTAACGACGAGAGCGGCGA270.1151582359464301No Hit
GCCCAGCCTCTGAGTTTTCTGTTCTATTTTTTTTTTTAACCCCAATTATCCT240.10236287639682676No Hit
ATGCTGGGAGAGTGAGACCCTGTCTTGAAAATAATAATAGGCTGGTCACAGT240.10236287639682676No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)